Archives of Advances in Biosciences

Vol. 17 No. 1 (2026)

Effect of Vitamin C antioxidant, Ag and TiO2 Nano-Particles on Homo sapiens Circular RNA 0001518 Expression in BALB/c Mice with Brain Tumor

Archives of Advances in Biosciences, Vol. 17 No. 1 (2026), 28 April 2026 , Page 1-8
https://doi.org/10.22037/aab.v17i1.51693

Abstract

Background and Aim: Brain cancer is a leading cause of mortality, posing significant treatment challenges. CircRNAs are emerging as innovative biomarkers and therapeutic targets for various diseases, including cancer. This study evaluates the half-maximal inhibitory concentration (IC50) of Vitamin C, Ag, and TiO2 nanoparticles (NPs) against the GL-261 tumor cell line and their impact on expression of hsa_circ_0001518 in cancerous and healthy mice.

This investigation aims to determine the impact of Vitamin C antioxidants and Ag and TiO2 NPs, individually and in combination, on the gene expression patterns of CircRNAs in brain cancer.

Methods: The GL-261 cell line was cultured and treated with Vitamin C (25, 50, 75, and 100 µg/ml), Ag (11, 24, 42.24, and 65 µg/ml), and TiO2 NPs (5, 7, 10.5, and 17.5 µg/ml). The MTT test was performed to determine cell viability. The expression of investigated circular RNA after treatment with the IC50 of Vitamin C in healthy and brain tumor-bearing mice was assessed by qRT-PCR.

Results: The IC50 values after 48h were 81.25 µg/ml for Vitamin C, 45.47 µg/ml for Ag NPs, and 8.73 µg/ml for TiO2 NPs. qRT-PCR results indicated that the combination of the antioxidant and NP significantly decreased circ0001518 expression in brain tumor-bearing animals more than any single treatment.

Conclusion: The combination of TiO2 and Vitamin C, as well as Ag and Vitamin C, significantly alters the expression of the investigated circ and shows potential as a novel therapeutic approach for brain cancer.

Keywords:
  • Brain Cancer
  • Vitamin C antioxidant
  • TiO2 nanoparticles
  • Ag Nanoparticles
  • hsa circ 0001518

How to Cite

Khojastehkiakola, S., Heshmati, M., Hashemi, M., & Entezari, M. (2026). Effect of Vitamin C antioxidant, Ag and TiO2 Nano-Particles on Homo sapiens Circular RNA 0001518 Expression in BALB/c Mice with Brain Tumor. Archives of Advances in Biosciences, 17(1), 1–8. https://doi.org/10.22037/aab.v17i1.51693

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