Altered Immune Responses in Mice After Receiving Nicotine-pulsed Mesenchymal Stem Cell-conditioned Medium
Archives of Advances in Biosciences,
Vol. 12 No. 4 (2021),
12 Mehr 2021
,
Page 1-11
https://doi.org/10.22037/aab.v12i4.35358
Abstract
Introduction: Previous investigations have documented that nicotine-pulsed mesenchymal
stem cells (MSCs) can induce an anti-inflammatory phenotype in some immune cells in vitro.
This study aimed to assess the effects of nicotine-pulsed MSCS in the function of immune
cells, macrophages, and lymphocytes of mice receiving these cels.
Materials and Methods: Bone marrow-derived MSCs (1.5×106) were seeded in a T75flask
and incubated with 0, .1, .5, or 1 μM nicotine until the cells reached 90% confluency.
Afterwards, immunophenotyping change, vitality, concentration of TGF-β, IL-10, and IDO
levels of the MSC-conditioned medium were examined. Correspondent to in vitro results, the
C57BL/6 mice intravenously received 400 μL of the conditioned medium of MSCs (CM),
conditioned medium of nicotine (.5 μM)-pulsed MSCs (CMN), or medium. After 12 h, the
lymphocytes, neutrophils, and peritoneal macrophages of the mice were isolated and their
function was evaluated ex vivo.
Results: The least effective dose concentration of nicotine that led to an anti-inflammatory
environment by the MSC-conditioned medium was 0.5 μM. Nicotine at this concentration
prompted a higher level of TGF-β, IDO concentration in the conditioned medium. However,
this concentration did not affect the MScs’ markers expressions or MScs’ vitality. T lymphocytes
isolated from the mice receiving CMN showed a significant decrease in proliferation rate. The
ratio of the IFN-γ gene expression to IL-4 gene expression in splenocytes was significantly
reduced in the mice receiving CMN compared to the mice receiving CM. The neutral red
uptake, respiratory burst, and nitric oxide production of the peritoneal macrophage only
decreased in the mice treated with CMN. These factors also decreased in neutrophils isolated
from mice receiving CM or CMN. However, these decreases were more prominent in the mice
treated with CMN.
Conclusion: Treatment of MSCs by nicotine may be a promising strategy to enhance the
immunomodulatory properties of these cells.
- Nicotine, Mesenchymal stem cells, Immunomodulation
How to Cite
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