Antibacterial and anticancer activity of a bioflavonoid fractionated from Allium Ascalonicum
Archives of Advances in Biosciences,
Vol. 9 No. 3 (2018),
3 July 2018
,
Page 1-8
https://doi.org/10.22037/jps.v9i3.19408
Abstract
Allium ascalonicum is a part of the diet of many populations of the world due to their long-held beliefs. A. ascalonicum extracts have been reported have antibacterial properties and prevent cancer cell proliferation. This study was conducted for the purpse of evaluating the anticancer and antibacterial activity of a flavonoid fraction isolated from A. ascalonicum bulbs. The HeLa and HUVEC cells were used as target cell line and some gram negative and positive bacteria were also targeted for antimicrobial activity. The A. ascalonicum plant was collected from the Zagros Mountains in the north of Dezful city- Iran, in September 2016 and confirmed by School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. The water extract of bulbs of this plant was extracted and the flavonoid fraction was isolated from aquous extract by ethyl acetate. The antibacterial and anticancer effects of isolated falavonoid were determined using MIC and MTT respectively. The best antibacterial effect of falvonoid extracted from A.ascalonicum was found against C. diphtheria. Furthermore, gentamicin resistant P.aeruginosa was the most resistant pathogenic bacterium. The MTT method showed that this fraction had a concentration-dependent anti-proliferative activity on HeLa cell lines and there was no cytotoxic effect against HUVEC cells. The inhibitory concentration 50% (IC50) values of the A. ascalonicum extract for Hela cell was 3 mg/mL but the treatment of HUVECs with the A. ascalonicum showed no considerable effect. The flavonoid fraction of A.ascalonicum bulbs had remarkable antibacterial and anticancer properties. Therefore, it could be used as an antibacterial and anticancer agent for control of cancers and infectious diseases.
- Anticancer
- Allium ascalonicum
- Allium sativum
- flavonoid
How to Cite
References
Hanelt P, Schultze Motel J, Fritsch R, Kruse J, Maass H, Ohle H, et al. Infrageneric grouping of Allium-the Gatersleben approach. The genus Allium-taxonomic problems and genetic resources Inst Pflanzengenetik & Kulturpflanzenforschung. Gatersleben 1992:107-123.
Takahama U, Hirota S. Deglucosidation of quercetin glucosides to the Anglican and the formation of antifungal agents by peroxidase-dependent oxidation of quercetin on browning of onion scales. Plant Cell Physiol 2000;41(9):1021-9.
Amin M , Kapandis B . Heat stable antimicrobial activity of Allium ascalonicum against bacteria and fungi. Indian J Exp Biol 2005;43:751-754.
Amiri MS, Joharchi MR. Ethnobotanical investigation of traditional medicinal plants commercialized in the markets of Mashhad, Iran. Avicenna J Phytomed 2013;3(3): 254-271.
Hakemi- Vala M, Mehrara M, Pourramezan M, Asgarpanah J, Rahimifard N, Khoshnood S et al. Comparison the antimicrobial effect of methanolic total extracts and petroleum ether fractions of flowering aerial parts of Glaucium vitellinum Boiss. & Buhse and Gaillonia aucheri Jaub. & Spach. Novelty Biomed 2017;5(1):249.
Hakemi-Vala M, Eslamzadeh A, Bejestani FB,
Asgarpanah J, Heidary M, Khoshnood S. Preliminary Evaluation of the Antimicrobial Activity of Total Extract and Fractions of Chloroform, Methanol, and Aqueous from the Aerial Parts of Salvia aegyptiaca. Avicenna Journal of Clinical Microbiology and Infection. 2017;4(3).
Razyfard H, Zarre S, Fritsch RM, Maroofi H. Four new species of Allium (Alliaceae) from Iran. Ann Bot Fenn 2011;48(4):352-360.
Heidary M, Hashemi A, Goudarzi H, Khoshnood S, Roshani M, Azimi H, et al. The antibacterial activity of Iranian plants extracts against metallo beta-lactamase producing Pseudomonas aeruginosa strains. J Paramed Sci 2016;7(1):13-19.
Hakemi-Vala M, Makhmor M, Kobarfar F, Kamalinejad M, Heidary M, Khoshnood S. Investigation of antimicrobial effect of Tribulus terrestris L. Against some gram positive and negative bacteria and Candida spp. Novelty Biomed 2014;2(3):85-90.
Block E, Birringer M, Jiang W, Nakahodo T, Thompson HJ, Toscano PJ, Uzar H, Zhang X, Zhu Z. Allium chemistry: synthesis, natural occurrence, biological activity, and chemistry of Se-alk (en) ylselenocysteines and their γ-glutamyl derivatives and oxidation products. J Agric Food Chem 2001;49(1):458-70.
Pandurangan V, Amanulla SSD, Ramanathan K. Anticancer efficacy of dry and fresh Allium ascalonicum (shallot) against HepG2 cell line. Natl J Physiol Pharm Pharmacol. 2016;6(3):196-199.
Azadi HG, Riazi GH, Ghaffari SM, Ahmadian S, Khalife TJ. Effects of Allium hirtifolium (Iranian shallot) and its allicin on microtubule and cancer cell lines. Afr J Biotechnol 2009; 8(19): 5030-5037.
Hamta A, Shariatzadeh S, Soleimani M, Tajali AM. Cytotoxicity effect of aquous and alcoholic total extract of shallot (Allium Ascalunicum) on cancer cell Derived from mammary Tumors in rat and cell line (4T1) in mouse, and comparison with taxol and Carboplatin Chemotherapy drugs. J cell tissue 2014;5(3): 253-61
Amin M, Montazeri EA, Mashhadizadeh MA, Sheikh AF. Characterization of shallot, an antimicrobial extract of Allium ascalonicum. Pak
J Med Sci 2009;25(6):948-52.
Amin M, Choghakabodi PM, Hamidi MA, Najafian M, Sheikh AF. In vitro antimicrobial activities of metabolites from vaginal
Lactobacillus strains against Clostridium perfringens isolated from a woman's vagina. J Chin Med Assoc 2017;80(1):29-33.
composition, antioxidative activity and cell viability effects of a Siberian pine (Pinus sibirica Du Tour) extract. Food Chem 2009;112(4):936-43.
Solowey E, Lichtenstein M, Sallon S, Paavilainen H, Solowey E, Lorberboum-Galski H. Evaluating medicinal plants for anticancer activity. Sci World J 2014.
Wang H, NG, T. Ascalin, a new anti-fungal peptide with human immunodeficiency virus type 1 reverse transcriptase-inhibiting activity from shallot bulbs. Peptides 2002;23(6):1025-29.
Ehsani A, Mahmoudi R, Zare P, Hasany A. Biochemical Properties and Antimicrobial effect of Allium ascalonicum and Pimpinella anisum
Essential Oils against Listeria Monocytogenes in White Brined Cheese. J Food Ind 2011;21(3):318-328
Negi PS. Plant extracts for the control of bacterial growth: Efficacy, stability and safety issues for food application. Int J Food Microbiol 2012;156(1):7-17.
Amin M, Segatoleslami S, Hashemzadeh M. Antimycobacterial activity of partial purified extract of Allium ascalonicum. Jundishapur J Microbiol 2009;4(2):144-147.
Sengupeta A, Ghosh S, Bhattacharjee S. Allium vegetables in cancer prevention: an overview. Asian Pac J Cancer Prev 2004;5(3):237-245.
Leelarungrayub N, Rattanapanone V, Chanarat N, Gebicki JM. Quantitative evaluation of the antioxidant properties of garlic and shallot preparations. Nutrition 2006;22(3):266-74.
Trakranrungsie N, Chatchawanchonteera A, Khunkitti W. Ethnoveterinary study for anti dermatophytic activity of Piper betle, Alpinia galanga and Allium ascalonicum extracts in vitro. Res Vet Sci 2008;84(1):80-84.
Mohammadi Motlagh HR, Mostafaie A, Mansouri K. Anticancer and anti-inflammatory activities of shallot. Arch Med Sci 2011;7(1):38-44.
- Abstract Viewed: 542 times
- PDF Downloaded: 227 times