Radiolabeling and Bio-distribution study of ICD-85 with Technetium-99m as a cancer treatment agent in mice
Archives of Advances in Biosciences,
Vol. 9 No. 2 (2018),
13 March 2018
,
Page 1-6
https://doi.org/10.22037/jps.v9i2.18792
Abstract
ICD-85 is a combination of three poly-peptides, ranging from 10,000 to 30,000 Dalton, derived from the venoms of an Iranian brown snake (Agkistrodon halys) and a yellow scorpion (Hemiscorpius lepturus). Labeling of this ICD-85 was successfully achieved with 99mTc, through direct method using SnF2 as reducing agent. Labeled ICD-85 was injected into mice to determine the excretion pathway. The results show that the maximum labeling yield (>75%) was obtained by using 30 μg of ICD-85 in phosphate buffer (60 μl, pH 7.1) at room temperature. Bio-distribution studies with radiolabeled ICD-85 shows moderate clearance of the complex from blood. The improvement of the immunotherapeutic treatment of cancer requires a better knowledge of the biological actions of the ICD-85 since tissue distribution studies are very important for clinical purpose.
- Radiolabeling
- Tc-99m
- Bio-distribution
- Venom
- ICD-85
How to Cite
References
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