Immunomics Approach to Develop an Immunogenic Polypeptide from Brucella abortus: Design and Recombinant Expression Develop an immunogenic polypeptide from Brucella abortus
Trends in Peptide and Protein Sciences,
Vol. 8 No. 1 (2023),
24 Dey 2023
,
Page 1-7 (e7)
https://doi.org/10.22037/tpps.v8i1.43825
Abstract
Brucellosis in livestock and its transmission to humans through the consumption of contaminated dairy products is an important issue. In this study, five immunogenic proteins were obtained from a 2DE gel analysis of Brucella abortus proteome after interaction with infected cattle's serum antibodies. These five proteins, MOXR family ATPase-α2, T9SS C-terminal Target domain-containing protein, Cobyric acid synthase, Hypothetical Protein, and Vir-B11 type IV Secretion Protein were introduced to the Brucella abortus proteome by MALDI-TOF MS/MS spectroscopy and then, analyzed with Mascot. Bioinformatics was applied to predict B and T cell epitopes, which were then randomly linked together to design a novel recombinant multi-epitope protein. The synthesized construct was transferred to E. coli BL21 and the expressed protein (ABOR) contained 549aa was confirmed with specific antibodies in infected cattle's serum.
HIGHLIGHTS
- Immunomics results of Brucella abortus demonstrated five immunogenic proteins.
- Antigenic epitopes were predicted and cloned in the pET22b expression vector.
- The expressed polypeptide was confirmed by interaction with antibodies in infected cattle's sera.
- Brucella abotus
- Immunoproteomics
- Recombinant proteins
How to Cite
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