Effect of Gold Nanoparticles and Hydroxypropylmethylcellulose on the Activity of Two Novel Designed Antimicrobial Peptides Effect of formulation components on the Antimicrobial Activity of AMP
Iranian Journal of Pharmaceutical Sciences,
Vol. 18 No. 4 (2022),
1 October 2022
,
Page 297-307
https://doi.org/10.22037/ijps.v18.41989
Abstract
Infectious wounds are one of the issues that significantly burden population lives and finances each year. Even though there have been significant breakthroughs in wound healing dressing, wound infections continue to be an issue. One of the latest techniques is using contemporary dressings containing antimicrobial agents to expedite wound healing and prevent infection. In the present study, the effect of gold nanoparticles (AuNPs) and hydroxypropylmethylcellulose (HPMC) as the main expedient of a hydrogel formulation on the bioactivity of two newly designed antimicrobial peptide (AMPs) was investigated. AuNPs were produced using two methods of chemical and biological synthesis. AuNPs were characterized individually and in the presence of AMPs in a stepwise manner. The antibacterial activity of these combinations against Staphylococcus aureus and Acinetobacter baumanii was evaluated. The results demonstrated that the stability of green-synthesized AuNPs was significantly superior to that of chemically synthesized AuNPs in presence of AMPs. In addition, the antibacterial activity of AMPs changed when combined with AuNPs and HPMC compared to its free state. This alteration was different based on the AMP identity and the combination composition. In the case of AMP1, designed based on regenerating islet-derived protein 3-alpha (REG3A), addition of AuNPs could enhance the antimicrobial activity. However, in the presence of another AMP (designed based on Cathelicidin-2), activity variations did not adopt with a distinct pattern. In general, the best antimicrobial activity was observed on the A. baumanii when a combination of green synthesized AuNPs, AMP derived from Cathelicidin-2 and HPMC was applied. In conclusion, since the inclusion of hydrogel and nanoparticles in the most combination conditions resulted in the efficacy reduction of AMPs, further efforts in selecting a suitable polymeric component should be made to develop an effective and inexpensive wound dressing formulation for this designed AMP.
- Peptide
- Hydrogel
- Nanoparticle
- Formulation
- Staphylococcus aureus
- Acinetobacter baumanii
How to Cite
References
2. Puthia M, Butrym M, Petrlova J, Strömdahl A-C, Andersson MÅ, Kjellström S, Schmidtchen A (2020) A dual-action peptide-containing hydrogel targets wound infection and inflammation. Science Translational Medicine 12:eaax6601
3. Lu Z, Gao J, He Q, Wu J, Liang D, Yang H, Chen R (2017) Enhanced antibacterial and wound healing activities of microporous chitosan-Ag/ZnO composite dressing. Carbohydrate polymers 156:460-469
4. Fan Y, Lu Q, Liang W, Wang Y, Zhou Y, Lang M (2021) Preparation and characterization of antibacterial polyvinyl alcohol/chitosan sponge and potential applied for wound dressing. European Polymer Journal 157:110619
5. Chin JS, Madden L, Chew SY, Becker DL (2019) Drug therapies and delivery mechanisms to treat perturbed skin wound healing. Advanced Drug Delivery Reviews 149:2-18
6. Nayab S, Aslam MA, Sajid S, Zafar N, Razaq M, Kanwar R (2022) A Review of Antimicrobial Peptides: Its Function, Mode of Action and Therapeutic Potential. International Journal of Peptide Research and Therapeutics 28:1-15
7. Chen WY, Chang HY, Lu JK, Huang YC, Harroun SG, Tseng YT, Li YJ, Huang CC, Chang HT (2015) Self‐assembly of antimicrobial peptides on gold nanodots: against multidrug‐resistant bacteria and wound‐healing application. Advanced Functional Materials 25:7189-7199
8. de Alteriis E, Maselli V, Falanga A, Galdiero S, Di Lella FM, Gesuele R, Guida M, Galdiero E (2018) Efficiency of gold nanoparticles coated with the antimicrobial peptide indolicidin against biofilm formation and development of Candida spp. clinical isolates. Infection and drug resistance 11:915
9. Otari SV, Patel SK, Jeong J-H, Lee JH, Lee J-K (2016) A green chemistry approach for synthesizing thermostable antimicrobial peptide-coated gold nanoparticles immobilized in an alginate biohydrogel. RSC advances 6:86808-86816
10. Casciaro B, Moros M, Rivera-Fernandez S, Bellelli A, Jesús M, Mangoni ML (2017) Gold-nanoparticles coated with the antimicrobial peptide esculentin-1a (1-21) NH2 as a reliable strategy for antipseudomonal drugs. Acta biomaterialia 47:170-181
11. Thapa RK, Diep DB, Tønnesen HH (2020) Topical antimicrobial peptide formulations for wound healing: Current developments and future prospects. Acta biomaterialia 103:52-67
12. Fathi F, Ghobeh M, Mahboubi A, Tabarzad M (2022) Controversy Between In Vitro Biological Activities of a Novel Designed Antimicrobial Peptide and Its In Silico Predicted Activities: Novel in silico designed AMP. Trends in Peptide and Protein Sciences 7:1-12 (e14). https://doi.org/10.22037/tpps.v7i.38346
13. Dong J, Carpinone PL, Pyrgiotakis G, Demokritou P, Moudgil BM (2020) Synthesis of precision gold nanoparticles using Turkevich method. KONA Powder and Particle Journal 37:224-232
14. Dananjaya S, Thao NT, Wijerathna H, Lee J, Edussuriya M, Choi D, Kumar RS (2020) In vitro and in vivo anticandidal efficacy of green synthesized gold nanoparticles using Spirulina maxima polysaccharide. Process Biochemistry 92:138-148
15. de Matos Fonseca J, Valencia GA, Soares LS, Dotto MER, Campos CEM, Moreira RdFPM, Fritz ARM (2020) Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films: Physicochemical and structural properties. International journal of biological macromolecules 151:944-956
16. Vieira ACF, de Matos Fonseca J, Menezes NMC, Monteiro AR, Valencia GA (2020) Active coatings based on hydroxypropyl methylcellulose and silver nanoparticles to extend the papaya (Carica papaya L.) shelf life. International Journal of Biological Macromolecules 164:489-498
17. Wadhwani P, Heidenreich N, Podeyn B, Bürck J, Ulrich AS (2017) Antibiotic gold: tethering of antimicrobial peptides to gold nanoparticles maintains conformational flexibility of peptides and improves trypsin susceptibility. Biomaterials science 5:817-827
18. Shah M, Badwaik V, Kherde Y, Waghwani HK, Modi T, Aguilar ZP, Rodgers H, Hamilton W, Marutharaj T, Webb C (2014) Gold nanoparticles: various methods of synthesis and antibacterial applications. Frontiers in Bioscience-Landmark 19:1320-1344
19. Shamaila S, Zafar N, Riaz S, Sharif R, Nazir J, Naseem S (2016) Gold nanoparticles: an efficient antimicrobial agent against enteric bacterial human pathogen. Nanomaterials 6:71
20. Hosny M, Fawzy M, Abdelfatah AM, Fawzy EE, Eltaweil AS (2021) Comparative study on the potentialities of two halophytic species in the green synthesis of gold nanoparticles and their anticancer, antioxidant and catalytic efficiencies. Advanced Powder Technology 32:3220-3233
21. Tao C (2018) Antimicrobial activity and toxicity of gold nanoparticles: Research progress, challenges and prospects. Letters in applied microbiology 67:537-543
22. Wang M, Meng Y, Zhu H, Hu Y, Xu C-P, Chao X, Li W, Li C, Pan C (2021) Green synthesized gold nanoparticles using Viola betonicifolia leaves extract: Characterization, antimicrobial, antioxidant, and cytobiocompatible activities. International Journal of Nanomedicine 16:7319
23. Rai A, Pinto S, Velho TR, Ferreira AF, Moita C, Trivedi U, Evangelista M, Comune M, Rumbaugh KP, Simões PN (2016) One-step synthesis of high-density peptide-conjugated gold nanoparticles with antimicrobial efficacy in a systemic infection model. Biomaterials 85:99-110
24. Dharmalingam K, Anandalakshmi R (2019) Fabrication, characterization and drug loading efficiency of citric acid crosslinked NaCMC-HPMC hydrogel films for wound healing drug delivery applications. International journal of biological macromolecules 134:815-829
25. Mohebian Z, Tajmohammadi I, Yavari Maroufi L, Ramezani S, Ghorbani M (2022) A novel aloe vera-loaded ethylcellulose/hydroxypropyl methylcellulose nanofibrous mat designed for wound healing application. Journal of Polymers and the Environment 30:867-877
26. kumar Kesavan S, Selvaraj D, Perumal S, Arunachalakasi A, Ganesan N, Chinnaiyan SK, Balaraman M (2022) Fabrication of hybrid povidone-iodine impregnated collagen-hydroxypropyl methylcellulose composite scaffolds for wound-healing application. Journal of Drug Delivery Science and Technology 70:103247
27. Howard A, O’Donoghue M, Feeney A, Sleator RD (2012) Acinetobacter baumannii: an emerging opportunistic pathogen. Virulence 3:243-250
28. Rajchakit U, Sarojini V (2017) Recent developments in antimicrobial-peptide-conjugated gold nanoparticles. Bioconjugate chemistry 28:2673-2686
29. Wang S, Zeng X, Yang Q, Qiao S (2016) Antimicrobial peptides as potential alternatives to antibiotics in food animal industry. International journal of molecular sciences 17:603
30. Allahverdiyev AM, Kon KV, Abamor ES, Bagirova M, Rafailovich M (2011) Coping with antibiotic resistance: combining nanoparticles with antibiotics and other antimicrobial agents. Expert review of anti-infective therapy 9:1035-1052
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