Production of Medium-Chain Length Polyhydroxyalkanoates by Pseudomonas citronellolis Grown in Apple Pulp Waste
Applied Food Biotechnology,
Vol. 6 No. 1 (2019),
2 January 2019
,
Page 71-82
https://doi.org/10.22037/afb.v6i1.21793
Abstract
Background and objective: Apple pulp waste generated by the fruit processing industry is a sugar-rich material with great potential to be used as a feedstock for production of value added microbial products. The aim of this work was to use this feedstock for the cultivation of Pseudomonas citronellolis and production of medium-chain-length polyhydroxyalkanoates, a natural elastomer.
Material and methods: The solid fraction of the apple pulp waste was discarded and the soluble fraction, rich in fructose (17.7 g l-1), glucose (7.5 g l-1) and sucrose (1.2 g l-1), was used for the batch bioreactor cultivation of Pseudomonas citronellolis NRRL B-2504.
Results and conclusion: Pseudomonas citronellolis reached a polymer content in the biomass of 30% wt and a volumetric productivity of 0.025 g l-1 h-1. The polymer was mainly composed of 3-hydroxydecanoate (68% mol) and 3-hydroxyoctanoate (22% mol), with minor contents of 3-hydroxydodecanoate (5% mol), 3-hydroxytetradecanoate (4% mol) and 3-hydroxyhexanoate (1% mol), and had a molecular weight of 3.7×105 Da. It presented glass transition and melting temperatures of -12 and 53°C, respectively, and a thermal degradation temperature of 296°C. The polymer’s films were dense, ductile and permeable to oxygen and carbon dioxide. These results demonstrated that apple pulp waste is a suitable feedstock for the production of a biopolymer with properties that render it a promising alternative to some synthetic petrochemical-derived polyesters.
Conflict of interest: The authors declare no conflict of interest.
- ▪ Apple pulp waste ▪ Medium-chain-length polyhydroxyalkanoates (mcl- PHA) ▪ Pseudomonas citronellolis ▪ Waste valorization
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