ISSN: 2345-5357
Summer
Vol. 3 No. 3 (2016)
Effect of Fatty Acids on Hydrophobicity of the Cell Membrane of Lactobacillus Species
Applied Food Biotechnology,
Vol. 3 No. 3 (2016),
,
Page 194-200
https://doi.org/10.22037/afb.v3i3.12375
Abstract
Background and Objectives: Probiotic bacteria are able to absorb fatty acids present in the culture medium and convert them into intracellular fatty acids, which may affect the physicochemical properties of probiotics. Subsequently, changing the composition of cellular fatty acids of probiotics improves the electron acceptance capacity of these microorganisms, and results in an increased adhesion to the intestinal mucus. In the present study, the effect of fatty acids on the physicochemical and adhesion properties of Lactobacillus species was investigated.
Materials and Methods: Seven fatty acids including palmitic, stearic, α-linolenic, γ-linolenic, oleic, linoleic and arachidonic acids were used for the enrichment of MRS medium. Afterwards, fatty acid content and adhesion property were measured using GC and spectrophotometer, respectively.
Results and Conclusion: The results showed that the type of microorganism and fatty acid had a significant effect (p≤0.05) on the adhesion property of probiotics. According to the results, the highest membrane fatty acid content was found for myristic and elaidic acid, and the lowest content for α-linoleic acid.
Conflict of interests: The authors declare no conflict of interest.
- Adhesion property
- Fatty acids
- Gas chromatography
- Hydrophobicity
- Probiotic
How to Cite
References
Salminen S, von Wright A, Morelli L, Marteau P, Brassart D, de Vos WM, et al. Demonstration of safety of probiotics -- a review. Int J Food Microbiol. 1998; 44(1-2):93-106.
Finlay BB, Falkow S. Common themes in microbial pathogenicity revisited. Microbiol Mol Biol Rev. 1997; 61(2):136-69.
Perea-Velez M, Hermans K, Verhoeven TL, Lebeer SE, Vanderleyden J, De Keersmaecker SC. Identification and characterization of starter lactic acid bacteria and probiotics from Columbian dairy products. J Appl Microbiol. 2007; 103(3):666-74.
Kankaanpaa P, Yang B, Kallio H, Isolauri E, Salminen S. Effects of polyunsaturated fatty acids in growth medium on lipid composition and on physicochemical surface properties of lactobacilli. Appl Environ Microbiol. 2004; 70(1):129-36.
Ouwehand AC, Isolauri E, Kirjavainen PV, Salminen SJ. Adhesion of four Bifidobacterium strains to human intestinal mucus from subjects in different age groups. FEMS Microbiol Lett. 1999; 172(1):61-4.
Kankaanpaa PE, Salminen SJ, Isolauri E, Lee YK. The influence of polyunsaturated fatty acids on probiotic growth and adhesion. FEMS Microbiol Lett. 2001; 194(2):149-53.
Wadstrom T, Andersson K, Sydow M, Axelsson L, Lindgren S, Gullmar B. Surface properties of lactobacilli isolated from the small intestine of pigs. J Appl Bacteriol. 1987; 62(6):513-20.
Partanen L, Marttinen N, Alatossava T. Fats and fatty acids as growth factors for Lactobacillus delbrueckii. Syst Appl Microbiol. 2001; 24(4):500-6.
Microbial ID. Microbial identification system operational manual. Microbial ID, Newark, Del, (1992).
Briandet R, Meylheuc T, Maher C, Bellon-Fontaine MN. Listeria monocytogenes Scott A: cell surface charge, hydrophobicity, and electron donor and acceptor characteristics under different environmental growth conditions. Appl Environ Microbiol. 1999; 65(12):5328-33.
Sanford P. A. Digestive system physiology. London, Edward Arnold, 1992.
Famularo G., Moretti S., Marcellini S., & De Simone C. Stimulation of immunity by probiotics. In R. Fuller, Probiotics. 2. Applications and practical aspects. London: Chapman& Hall, 1997:133-161.
O'Halloran S., Feeney M., Morrissey D., Murphy L., Thornton G.,Shanahan F., O'Sullivan G. C., & Collins J. K. Adhesion of potential probiotic bacteria to human epithelial cell lines. PBS 35.Functional foods: designer foods for the future. Ireland: Cork, 1997.
Elliott SN, Buret A, McKnight W, Miller MJ, Wallace JL. Bacteria rapidly colonize and modulate healing of gastric ulcers in rats. Am J Physiol. 1998; 275(3 Pt 1):G425-32.
Rementzis J, Samelis J. Rapid GC analysis of cellular fatty acids for characterizing Lactobacillus sake and Lact. curvatus strains of meat origin. Lett Appl Microbiol. 1996; 23(6):379-84.
Dionisi F, Golay PA, Elli M, Fay LB. Stability of cyclopropane and conjugated linoleic acids during fatty acid quantification in lactic acid bacteria. Lipids. 1999; 34(10):1107-15.
Murga ML, Cabrera GM, De Valdez GF, Disalvo A, Seldes AM. Influence of growth temperature on cryotolerance and lipid composition of Lactobacillus acidophilus. J Appl Microbiol. 2000; 88(2):342-8.
Guerzoni ME, Lanciotti R, Cocconcelli PS. Alteration in cellular fatty acid composition as a response to salt, acid, oxidative and thermal stresses in Lactobacillus helveticus. Microbiology. 2001; 147(Pt 8):2255-64.
Johnsson T, Nikkila P, Toivonen L, Rosenqvist H, Laakso S. Cellular Fatty Acid profiles of lactobacillus and lactococcus strains in relation to the oleic Acid content of the cultivation medium. Appl Environ Microbiol. 1995; 61(12):4497-9.
Kirjavainen PV, Ouwehand AC, Isolauri E, Salminen SJ.The ability of probiotic bacteria to bind to human intestinal mucus. FEMS Microbiol Lett. 1998; 167(2):185-9.
Suutari M, Laakso S. Temperature adaptation in Lactobacillus fermentum: interconversions of oleic, vaccenic and dihydrosterulic acids. J Gen Microbiol. 1992; 138(3):445-50.
- Abstract Viewed: 855 times
- PDF Downloaded: 677 times
