The Effect of Fe203 Nanoparticles on Sperm Parameters and the Expression of Mitochondrial Apoptotic Pathway Genes in Rats With Scrotal Hyperthermia
Archives of Advances in Biosciences,
Vol. 13 No. 1 (2022),
1 January 2022
,
Page 1-8
https://doi.org/10.22037/aab.v13i.36734
Abstract
Introduction: Hyperthermia can cause infertility in men following an increase in testicular
temperature. Oxidative stress has been found to be one of its major causes. In the present study,
the effects of iron superoxide nanoparticles on the expression of Bcl-2 and Bax family genes
were studied.
Materials and Methods: A total of 48 adult rats were purchased from the Pasteur Institute
of Iran. The rats were later divided into 4 groups: control group, control group receiving
superoxide nanoparticles (Fe2O3), hyperthermia group, h’:yperthermia group receiving
superoxide nanoparticles (Fe2O3). After RNA extraction, evaluating the sperm parameters and
the expression of Bax and Bcl-2 genes was examined using RT-PCR technique.
Results: Exposure to iron superoxide nanoparticles (Fe2O3) decreased sperm parameters,
increased proapoptotic BAX gene and decreased expression of BCL2 anti-apoptotic gene.
Conclusion: Exposure to nanoparticles by reducing sperm parameters and increasing apoptosis
has a negative effect on fertility. The association between infertility and testicular hyperthermia
is becoming increasingly apparent; administration of iron superoxide (Fe2O3) nanoparticles
can have significant effects on male infertility. Moreover, green synthesis of nanoparticles is
also recommended in this field.
- Rat, Bax, Bcl-2, Scrotal Hyperthermia.
How to Cite
References
Precone V, Cannarella R, Paolacci S, Busetto GM, Beccari T, Stuppia L, et al. Male infertility diagnosis: Improvement of genetic analysis performance by the introduction of pre-diagnostic genes in a next-generation sequencing custom-made panel. Front Endocrinol (Lausanne). 2021; 11:605237. [DOI:10.3389/fendo.2020.605237] [PMID] [PMCID]
Jungwirth A, Giwercman A, Tournaye H, Diemer T, Kopa Z, Dohle G, et al. European Association of Urology guidelines on Male Infertility: The 2012 update. Eur Urol. 2012; 62(2):324-32. [DOI:10.1016/j.eururo.2012.04.048] [PMID]
World Health Organization. [Laboratory manual of the WHO for the examination of human semen and sperm-cervical mucus interaction (Italian)]. Ann Ist Super Sanita. 2001; 37(1):I-XII, 1-123. [PMID]
de Kretser DM. Male infertility. lancet. 1997; 349(9054):787-90. [DOI:10.1016/S0140-6736(96)08341-9]
Hamada A, Esteves SC, Agarwal A, Androfert B, Campinas B. Unexplained male infertility: Potential causes and management. Hum Androl. 2011; 1(1):2-16. [DOI:10.1097/01.XHA.0000397686.82729.09]
Mieusset R. Scrotal hyperthermia; etiologic factors: Facts and hypotheses. In: Zorgniotti AW, editor. Temperature and environmental effects on the testis. Advances in experimental medicine and biology. Boston: Springer; 1991. [DOI:10.1007/978-1-4684-5913-5_22 ]
Laan M, Kasak L, Punab M. Translational aspects of novel findings in genetics of male infertility-status quo 2021. Br Med Bull. 2021; 140(1):5-22. [DOI:10.1093/bmb/ldab025] [PMID] [PMCID]
Paul C, Teng S, Saunders PT. A single, mild, transient scrotal heat stress causes hypoxia and oxidative stress in mouse testes, which induces germ cell death. Biol Reprod. 2009; 80(5):913-9. [DOI:10.1095/biolreprod.108.071779] [PMID] [PMCID]
Shiraishi K, Matsuyama H, Takihara H. Pathophysiology of varicocele in male infertility in the era of assisted reproductive technology. Int J Urol. 2012; 19(6):538-50. [DOI:10.1111/j.1442-2042.2012.02982.x] [PMID]
Qari SA, Alahmadi AA, Ali SS, Mohammedsaleh ZM, Ibrahim RFA, El-Shitany NA. Effect of prolonged whole-body hyperthermia on adult male rat testes and the protective role of vitamin C and E: A histological and biochemical study. Andrologia. 2021; 53(7):e14075. [DOI:10.1111/and.14075] [PMID]
Khan I, Saeed K, Khan I. Nanoparticles: Properties, applications and toxicities. Arab J Chem. 2019; 12(7):908-31. [DOI:10.1016/j.arabjc.2017.05.011]
Iftikhar M, Noureen A, Uzair M, Jabeen F, Abdel Daim M, Cappello T. Perspectives of nanoparticles in male infertility: Evidence for induced abnormalities in sperm production. Int J Environ Res Public Health. 2021; 18(4):1758. [DOI:10.3390/ijerph18041758] [PMID] [PMCID]
Verma A, Stellacci F. Effect of surface properties on nanoparticle-cell interactions. Small. 2010; 6(1):12-21. [DOI:10.1002/smll.200901158] [PMID]
Iftikhar M, Noureen A, Uzair M, Jabeen F, Abdel Daim M, Cappello T. Perspectives of nanoparticles in male infertility: Evidence for induced abnormalities in sperm production. Int J Environ Res Public Health. 2021; 18(4):1758. [DOI:10.3390/ijerph18041758] [PMID] [PMCID]
Sharma A, Minhas S, Dhillo WS, Jayasena CN. Male infertility due to testicular disorders. J Clin Endocrinol Metab. 2021; 106(2):e442-59. [DOI:10.1210/clinem/dgaa781] [PMID] [PMCID]
Walczak-Jedrzejowska R, Wolski JK, Slowikowska-Hilczer J. The role of oxidative stress and antioxidants in male fertility. Cent European J Urol. 2013; 66(1):60-7. [DOI:10.5173/ceju.2013.01.art19] [PMID] [PMCID]
Agarwal A, Baskaran S, Parekh N, Cho CL, Henkel R, Vij S, et al. Male infertility. Lancet. 2021; 397(10271):319-33. [DOI:10.1016/S0140-6736(20)32667-2]
Wu YQ, Rao M, Hu SF, Ke DD, Zhu CH, Xia W. Effect of transient scrotal hyperthermia on human sperm: An iTRAQ-based proteomic analysis. Reprod Biol Endocrinol. 2020; 18(1):83. [DOI:10.1186/s12958-020-00640-w] [PMID] [PMCID]
Vera Y, Diaz-Romero M, Rodriguez S, Lue Y, Wang C, Swerdloff RS, et al. Mitochondria-dependent pathway is involved in heat-induced male germ cell death: Lessons from mutant mice. Biol Reprod. 2004; 70(5):1534-40. [DOI:10.1095/biolreprod.103.024661] [PMID]
Cai H, Ren Y, Li XX, Yang JL, Zhang CP, Chen M, et al. Scrotal heat stress causes a transient alteration in tight junctions and induction of TGF-ß expression. Int J Androl. 2011; 34(4):352-62. [DOI:10.1111/j.1365-2605.2010.01089.x] [PMID]
Sun X, Chen X, Wang S, Zhang J, Wu B, Qin G. Protective effect of ursolic acid in prunella vulgaris L. on LPS-induced asthenozoospermia via Bcl-2/Bax apoptosis signaling pathway. Curr Pharm Biotechnol. 2021; 22(14):1953-9. [DOI:10.2174/1389201021666201027155413] [PMID]
Rao M, Xia W, Yang J, Hu LX, Hu SF, Lei H, et al. Transient scrotal hyperthermia affects human sperm DNA integrity, sperm apoptosis, and sperm protein expression. Andrology. 2016; 4(6):1054-63. [DOI:10.1111/andr.12228] [PMID]
Li XX, Chen SR, Shen B, Yang JL, Ji SY, Wen Q, et al. The heat-induced reversible change in the blood-testis barrier (BTB) is regulated by the androgen receptor (AR) via the partitioning-defective protein (Par) polarity complex in the mouse. Biol Reprod. 2013; 89(1):12. [DOI:10.1095/biolreprod.113.109405]
Rockett JC, Mapp FL, Garges JB, Luft JC, Mori C, Dix DJ. Effects of hyperthermia on spermatogenesis, apoptosis, gene expression, and fertility in adult male mice. Biol Reprod. 2001; 65(1):229-39. [DOI:10.1095/biolreprod65.1.229] [PMID]
Wahajuddin, Arora S. Superparamagnetic iron oxide nanoparticles: Magnetic nanoplatforms as drug carriers. Int J Nanomedicine. 2012; 7:3445-71. [DOI:10.2147/IJN.S30320] [PMID] [PMCID]
Rugiel M, Drozdz A, Matusiak K, Setkowicz Z, Klodowski K, Chwiej J. Organ metallome processed with chemometric methods enable the determination of elements that may serve as markers of exposure to iron oxide nanoparticles in male rats. Biol Trace Elem Res. 2020; 198(2):602-16. [DOI:10.1007/s12011-020-02104-z] [PMID] [PMCID]
Rao M, Zhao XL, Yang J, Hu SF, Lei H, Xia W, et al. Effect of transient scrotal hyperthermia on sperm parameters, seminal plasma biochemical markers, and oxidative stress in men. Asian J Androl. 2015; 17(4):668-75. [DOI:10.4103/1008-682X.146967] [PMID] [PMCID]
Karimi S, Tabatabaei SN, Gutleb AC, Ghaffari Novin M, Ebrahimzadeh-Bideskan A, Shams Mofarahe Z. The effect of PEGylated iron oxide nanoparticles on sheep ovarian tissue: An ex-vivo nanosafety study. Heliyon. 2020; 6(9):e04862. [DOI:10.1016/j.heliyon.2020.e04862] [PMID] [PMCID]
Moridian M, Khorsandi L, Talebi AR. Morphometric and stereological assessment of the effects of zinc oxide nanoparticles on the mouse testicular tissue. Bratisl Lek Listy. 2015; 116(5):321-5. [DOI:10.4149/BLL_2015_060] [PMID]
Yoo E, Cheng HA, Nardacci LE, Beaman DJ, Drinnan CT, Lee C, et al. Activatable interpolymer complex-superparamagnetic iron oxide nanoparticles as magnetic resonance contrast agents sensitive to oxidative stress. Colloids Surf B Biointerfaces. 2017; 158:578-88. [DOI:10.1016/j.colsurfb.2017.07.025] [PMID] [PMCID]
Arjaghi SK, Alasl MK, Sajjadi N, Fataei E, Rajaei GE. Green synthesis of iron oxide nanoparticles by RS lichen extract and its application in removing heavy metals of lead and cadmium. Biol Trace Elem Res. 2021; 199(2):763-8. [DOI:10.1007/s12011-020-02170-3] [PMID]
Yadwade R, Kirtiwar S, Ankamwar B. A review on green synthesis and applications of iron oxide nanoparticles. J Nanosci Nanotechnol. 2021; 21(12):5812-34. [DOI:10.1166/jnn.2021.19285] [PMID]
- Abstract Viewed: 388 times
- PDF Downloaded: 39 times
- MP3 Downloaded: 9 times