Long-term Evaluation of the Effect of Platelet-rich Fibrin on Cartilage Tissue Regeneration: An Animal Model Study
Regeneration, Reconstruction & Restoration (Triple R),
Vol. 5 (2020),
24 March 2020
,
Page e27
https://doi.org/10.22037/rrr.v5i.33067
Abstract
Introduction: Autologous cartilage graft in rhinoplasty is associated with common challenges, such as resorption, inflammation, and bone metaplasia. This study aimed to assess platelet-rich fibrin's long-term effect, as a superphysiological source of biomolecules, on cartilage regeneration in an animal model.
Material and Methods: Cartilage and fascia were excised autologously from the ear and leg of thirty-three Wistar rats. They then transplanted with cartilage alone (control group), cartilage wrapped with fascia (fascia group), and cartilage wrapped with PRF (fibrin group) autologously and subcutaneously.
Result: The histological results demonstrated no significant difference in chondrocyte viability, fibrosis, and resorption between three groups after six months. However, other parameters, including calcification, bone metaplasia, infection, and basophilia, were lower in the fibrin group.
Conclusion: This study concluded that PRF could be an appropriate autologous cell source for cartilage regeneration and nasal augmentation in the long term.
Keywords:
- Cartilage
- Regeneration
- PRF
- Rhinoplasty
How to Cite
Goljanian Tabrizi, A., Hashemi , H. ., Mohsenifar , Z. ., & Bohlouli, M. (2021). Long-term Evaluation of the Effect of Platelet-rich Fibrin on Cartilage Tissue Regeneration: An Animal Model Study. Regeneration, Reconstruction & Restoration (Triple R), 5, e27. https://doi.org/10.22037/rrr.v5i.33067
References
1. Kreutzer C, Hoehne J, Gubisch W, Rezaeian F, Haack S. Free diced cartilage: a new application of diced cartilage grafts in primary and secondary rhinoplasty. Plast Reconstr Surg. 2017;140(3):461-70.
2. Brenner KA, McConnell MP, Evans GR, Calvert JW. Survival of diced cartilage grafts: an experimental study. Plast Reconstr Surg. 2006;117(1):105-15.
3. Gordon CR, Alghoul M, Goldberg JS, Habal MB, Papay F. Diced cartilage grafts wrapped in AlloDerm for dorsal nasal augmentation. J Craniofac Surg. 2011;22(4):1196-9.
4. Göral A, Aslan C, Bolat Küçükzeybek B, Işık D, Hoşnuter M, Durgun M. Platelet-rich fibrin improves the viability of diced cartilage grafts in a rabbit model. Aesthetic Surg J. 2016;36(4):NP153-NP62.
5. Kazikdas KC, Ergur B, Tugyan K, Guneli E, Kaya D, Sahan M. Viability of crushed and diced cartilage grafts wrapped in oxidized regenerated cellulose and esterified hyaluronic acid: an experimental study. Laryngoscope. 2007;117(10):1728-34.
6. Firat C, Gurlek A, Aydn NE. Viability of cartilage grafts in various forms. J Craniofac Surg. 2011;22(5):1666-70.
7. Kemaloğlu CA, Tekin Y. A comparison of diced cartilage grafts wrapped in perichondrium versus fascia. Aesthetic Plast Surg. 2014;38(6):1164-8.
8. Liao J-L, Chen J, He B, Chen Y, Xu J-Q, Xie H-J, et al. Viability and biomechanics of diced cartilage blended with platelet-rich plasma and wrapped with poly (lactic-co-glycolic) acid membrane. J Craniofac Surg. 2017;28(6):1418-24.
9. Singh S, Sood T, Sabarigirish K, Ramakrishnan N, Rajguru R, Kumar KM. A New Era of diced cartilage rhinoplasty: Our experience. INDIAN J OTOLARYNGOL. 2015;67(4):338-40.
10. Tasman A-J. Advances in nasal dorsal augmentation with diced cartilage. Curr Opin Otolaryngol Head Neck Surg. 2013;21(4):365-71.
11. Choukroun J, Diss A, Simonpieri A, Girard M-O, Schoeffler C, Dohan SL, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 2006;101(3):e56-e60.
12. Habesoglu M, Oysu C, Sahin S, Sahin-Yilmaz A, Korkmaz D, Tosun A, et al. Platelet-rich fibrin plays a role on healing of acute-traumatic ear drum perforation. J Craniofac Surg. 2014;25(6):2056-8.
13. Bastami F, Khojasteh A. Use of leukocyte-and platelet-rich fibrin for bone regeneration: a systematic review. Regeneration, Reconstruction & Restoration. 2016;1(2):47-68.
14. Abd El Raouf M, Wang X, Miusi S, Chai J, Mohamed AbdEl-Aal AB, Nefissa Helmy MM, et al. Injectable-platelet rich fibrin using the low speed centrifugation concept improves cartilage regeneration when compared to platelet-rich plasma. Platelets. 2019;30(2):213-21.
15. Tarhan E, Cakmak O, Ozdemir BH, Akdogan V, Suren D. Comparison of AlloDerm, fat, fascia, cartilage, and dermal grafts in rabbits. Archives of Facial Plastic Surgery. 2008;10(3):187-93.
16. Güler İ, Billur D, Aydin S, Kocatürk S. Efficacy of platelet‐rich fibrin matrix on viability of diced cartilage grafts in a rabbit model. Laryngoscope. 2015;125(3):E104-E11.
17. Saluja H, Dehane V, Mahindra U. Platelet-Rich fibrin: A second generation platelet concentrate and a new friend of oral and maxillofacial surgeons. Ann Maxillofac Surg. 2011;1(1):53.
18. Manafi A. Effects of platelet-rich plasma on cartilage grafts in rabbits as an animal model. WJPS. 2012;1(2):91.
19. Liu Q, Huang Y, Lan Y, Zuo Q, Li C, Zhang Y, et al. Acceleration of skin regeneration in full‐thickness burns by incorporation of bFGF‐loaded alginate microspheres into a CMCS–PVA hydrogel. J TISSUE ENG REGEN M. 2017;11(5):1562-73.
20. Mehendale HM. Role of Tissue Repair and Death Proteins in Liver Injury. Drug-Induced Liver Disease: Elsevier; 2013. p. 195-213.
21. Xu F, Yang Y, Yang T, Dai T, Shao X, Xu H, et al. The use of allogenic adipose-derived stem cells in combination with platelet-rich fibrin for the treatment of cartilage defects in rabbit ear. Am J Transl Res. 2018;10(6):1900.
22. Wong C-C, Chen C-H, Chan WP, Chiu L-H, Ho W-P, Hsieh F-J, et al. Single-stage cartilage repair using platelet-rich fibrin scaffolds with autologous cartilaginous grafts. Am J Sports Med. 2017;45(13):3128-42.
23. Bahmanpour S, Ghasemi M, Sadeghi-Naini M, Kashani IR. Effects of platelet-rich plasma & platelet-rich fibrin with and without stromal cell-derived factor-1 on repairing full-thickness cartilage defects in knees of rabbits. IJMS. 2016;41(6):507.
24. Kemaloğlu CA, Tekin Y. The effect of sildenafil on fascia‐wrapped diced cartilage grafts. Laryngoscope. 2015;125(5):E168-E72.
25. Topkara A, Özkan A, Özcan RH, Öksüz M, Akbulut M. Effect of concentrated growth factor on survival of diced cartilage graft. Aesthetic Surg J. 2016;36(10):1176-87.
26. Dubay DA, Wang X, Kirk S, Adamson B, Robson MC, Franz MG. Fascial fibroblast kinetic activity is increased during abdominal wall repair compared to dermal fibroblasts. WOUND REPAIR REGEN. 2004;12(5):539-45.
27. Food, Administration D. Regulatory considerations for human cells, tissues, and cellular and tissue‐based products: Minimal manipulation and homologous use; guidance for industry and food and drug administration staff; availability. FDA. 2017;82(221):54290-2.
28. Brandl A, Angele P, Roll C, Prantl L, Kujat R, Kinner B. Influence of the growth factors PDGF‐BB, TGF‐β1 and bFGF on the replicative aging of human articular chondrocytes during in vitro expansion. Journal of Orthopaedic Research. 2010;28(3):354-60.
2. Brenner KA, McConnell MP, Evans GR, Calvert JW. Survival of diced cartilage grafts: an experimental study. Plast Reconstr Surg. 2006;117(1):105-15.
3. Gordon CR, Alghoul M, Goldberg JS, Habal MB, Papay F. Diced cartilage grafts wrapped in AlloDerm for dorsal nasal augmentation. J Craniofac Surg. 2011;22(4):1196-9.
4. Göral A, Aslan C, Bolat Küçükzeybek B, Işık D, Hoşnuter M, Durgun M. Platelet-rich fibrin improves the viability of diced cartilage grafts in a rabbit model. Aesthetic Surg J. 2016;36(4):NP153-NP62.
5. Kazikdas KC, Ergur B, Tugyan K, Guneli E, Kaya D, Sahan M. Viability of crushed and diced cartilage grafts wrapped in oxidized regenerated cellulose and esterified hyaluronic acid: an experimental study. Laryngoscope. 2007;117(10):1728-34.
6. Firat C, Gurlek A, Aydn NE. Viability of cartilage grafts in various forms. J Craniofac Surg. 2011;22(5):1666-70.
7. Kemaloğlu CA, Tekin Y. A comparison of diced cartilage grafts wrapped in perichondrium versus fascia. Aesthetic Plast Surg. 2014;38(6):1164-8.
8. Liao J-L, Chen J, He B, Chen Y, Xu J-Q, Xie H-J, et al. Viability and biomechanics of diced cartilage blended with platelet-rich plasma and wrapped with poly (lactic-co-glycolic) acid membrane. J Craniofac Surg. 2017;28(6):1418-24.
9. Singh S, Sood T, Sabarigirish K, Ramakrishnan N, Rajguru R, Kumar KM. A New Era of diced cartilage rhinoplasty: Our experience. INDIAN J OTOLARYNGOL. 2015;67(4):338-40.
10. Tasman A-J. Advances in nasal dorsal augmentation with diced cartilage. Curr Opin Otolaryngol Head Neck Surg. 2013;21(4):365-71.
11. Choukroun J, Diss A, Simonpieri A, Girard M-O, Schoeffler C, Dohan SL, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 2006;101(3):e56-e60.
12. Habesoglu M, Oysu C, Sahin S, Sahin-Yilmaz A, Korkmaz D, Tosun A, et al. Platelet-rich fibrin plays a role on healing of acute-traumatic ear drum perforation. J Craniofac Surg. 2014;25(6):2056-8.
13. Bastami F, Khojasteh A. Use of leukocyte-and platelet-rich fibrin for bone regeneration: a systematic review. Regeneration, Reconstruction & Restoration. 2016;1(2):47-68.
14. Abd El Raouf M, Wang X, Miusi S, Chai J, Mohamed AbdEl-Aal AB, Nefissa Helmy MM, et al. Injectable-platelet rich fibrin using the low speed centrifugation concept improves cartilage regeneration when compared to platelet-rich plasma. Platelets. 2019;30(2):213-21.
15. Tarhan E, Cakmak O, Ozdemir BH, Akdogan V, Suren D. Comparison of AlloDerm, fat, fascia, cartilage, and dermal grafts in rabbits. Archives of Facial Plastic Surgery. 2008;10(3):187-93.
16. Güler İ, Billur D, Aydin S, Kocatürk S. Efficacy of platelet‐rich fibrin matrix on viability of diced cartilage grafts in a rabbit model. Laryngoscope. 2015;125(3):E104-E11.
17. Saluja H, Dehane V, Mahindra U. Platelet-Rich fibrin: A second generation platelet concentrate and a new friend of oral and maxillofacial surgeons. Ann Maxillofac Surg. 2011;1(1):53.
18. Manafi A. Effects of platelet-rich plasma on cartilage grafts in rabbits as an animal model. WJPS. 2012;1(2):91.
19. Liu Q, Huang Y, Lan Y, Zuo Q, Li C, Zhang Y, et al. Acceleration of skin regeneration in full‐thickness burns by incorporation of bFGF‐loaded alginate microspheres into a CMCS–PVA hydrogel. J TISSUE ENG REGEN M. 2017;11(5):1562-73.
20. Mehendale HM. Role of Tissue Repair and Death Proteins in Liver Injury. Drug-Induced Liver Disease: Elsevier; 2013. p. 195-213.
21. Xu F, Yang Y, Yang T, Dai T, Shao X, Xu H, et al. The use of allogenic adipose-derived stem cells in combination with platelet-rich fibrin for the treatment of cartilage defects in rabbit ear. Am J Transl Res. 2018;10(6):1900.
22. Wong C-C, Chen C-H, Chan WP, Chiu L-H, Ho W-P, Hsieh F-J, et al. Single-stage cartilage repair using platelet-rich fibrin scaffolds with autologous cartilaginous grafts. Am J Sports Med. 2017;45(13):3128-42.
23. Bahmanpour S, Ghasemi M, Sadeghi-Naini M, Kashani IR. Effects of platelet-rich plasma & platelet-rich fibrin with and without stromal cell-derived factor-1 on repairing full-thickness cartilage defects in knees of rabbits. IJMS. 2016;41(6):507.
24. Kemaloğlu CA, Tekin Y. The effect of sildenafil on fascia‐wrapped diced cartilage grafts. Laryngoscope. 2015;125(5):E168-E72.
25. Topkara A, Özkan A, Özcan RH, Öksüz M, Akbulut M. Effect of concentrated growth factor on survival of diced cartilage graft. Aesthetic Surg J. 2016;36(10):1176-87.
26. Dubay DA, Wang X, Kirk S, Adamson B, Robson MC, Franz MG. Fascial fibroblast kinetic activity is increased during abdominal wall repair compared to dermal fibroblasts. WOUND REPAIR REGEN. 2004;12(5):539-45.
27. Food, Administration D. Regulatory considerations for human cells, tissues, and cellular and tissue‐based products: Minimal manipulation and homologous use; guidance for industry and food and drug administration staff; availability. FDA. 2017;82(221):54290-2.
28. Brandl A, Angele P, Roll C, Prantl L, Kujat R, Kinner B. Influence of the growth factors PDGF‐BB, TGF‐β1 and bFGF on the replicative aging of human articular chondrocytes during in vitro expansion. Journal of Orthopaedic Research. 2010;28(3):354-60.
- Abstract Viewed: 209 times
- PDF Downloaded: 109 times