Evaluation of correlation of serum vitamin B12 with proteinuria in type 2 Diabetes Mellitus patients
School of Medicine Students' Journal,
Vol. 3 No. 1 (2021),
1 March 2021
Background and aims: Diabetic nephropathy is one of the leading causes of end stage renal disease (ESRD) in the world. This study was conducted to investigate the correlation of B12 deficiency with presence of proteinuria in patients with Diabetes Mellitus.
Materials and Methods: This study was performed on patients with type 2 Diabetes Mellitus. The inclusion criteria were: HbA1C ≤8%, absence of hypertension or controlled blood pressure (BP<140/90) in hypertensive patients, no smoking, absence of any recent stressful illness such as MI, CVA and causes of proteinuria other than diabetic nephropathy. The serum level of B12 and the amount of urine protein–to-creatinine ratio (UPCR) were measured, and correlation between b12 deficiency and presence of proteinuria was assessed using Spearman correlation test.
Results: In this study 78 patients (44.87% women and 55.13% men) were examined. There was an inverse and weak correlation between the serum B12 deficiency and presence of proteinuria (r = -0 / 104), which was not statistically significant (p = 0.254). Due to the statistically significant difference between two groups with and without proteinuria in antiproteinuric drugs, as well as Metformin use and chronic kidney disease (CKD) stage, a subgroup analysis was performed in each of these subgroups. There was no correlation between the B12 deficiency and presence of proteinuria.
Conclusion: The findings revealed no statistically significant correlation between the serum B12 deficiency and presence of proteinuria.
- serum B12 level
- Diabetes mellitus
How to Cite
2. Pflipsen MC, Oh RC, Saguil A, Seehusen DA, Seaquist D, Topolski R. The prevalence of vitamin B(12) deficiency in patients with type 2 diabetes: a cross-sectional study. J Am Board Fam Med. 2009;22(5):528-34.
3. Valdés-Ramos R, Ana Laura G-L, Beatriz Elina M-C, Alejandra Donaji B-A. Vitamins and type 2 diabetes mellitus. Endocrine, Metabolic & Immune Disorders-Drug Targets (Formerly Current Drug Targets-Immune, Endocrine & Metabolic Disorders). 2015;15(1):54-63.
4. Sato Y, Ouchi K, Funase Y, Yamauchi K, Aizawa T. Relationship between metformin use, vitamin B12 deficiency, hyperhomocysteinemia and vascular complications in patients with type 2 diabetes. Endocrine journal. 2013;60(12):1275-80.
5. Nix WA, Zirwes R, Bangert V, Kaiser RP, Schilling M, Hostalek U, et al. Vitamin B status in patients with type 2 diabetes mellitus with and without incipient nephropathy. Diabetes Res Clin Pract. 2015;107(1):157-65.
6. Ahmed MA, Muntingh G, Rheeder P. Vitamin B12 deficiency in metformin-treated type-2 diabetes patients, prevalence and association with peripheral neuropathy. BMC Pharmacol Toxicol. 2016;17(1):44.
7. Chen CH, Yang WC, Hsiao YH, Huang SC, Huang YC. High homocysteine, low vitamin B-6, and increased oxidative stress are independently associated with the risk of chronic kidney disease. Nutrition. 2016;32(2):236-41.
8. O'Leary F, Samman S. Vitamin B12 in health and disease. Nutrients. 2010;2(3):299-316.
9. Amin HK, El-Sayed MI, Leheta OF. Homocysteine as a predictive biomarker in early diagnosis of renal failure susceptibility and prognostic diagnosis for end stages renal disease. Ren Fail. 2016;38(8):1267-75.
10. Bherwani S, Ahirwar AK, Saumya AS, Sandhya AS, Prajapat B, Patel S, et al. The study of association of Vitamin B12 deficiency in type 2 diabetes mellitus with and without diabetic nephropathy in North Indian Population. Diabetes Metab Syndr. 2017;11 Suppl 1:S365-S8.
11. McMahon GM, Hwang S-J, Tanner RM, Jacques PF, Selhub J, Muntner P, et al. The association between vitamin B12, albuminuria and reduced kidney function: an observational cohort study. BMC nephrology. 2015;16(1):7.
12. House AA, Eliasziw M, Cattran DC, et al. Effect of b-vitamin therapy on progression of diabetic nephropathy: A randomized controlled trial. JAMA. 2010;303(16):1603-9.
13. Song Y, Cook NR, Albert CM, Van Denburgh M, Manson JE. Effect of homocysteine-lowering treatment with folic Acid and B vitamins on risk of type 2 diabetes in women: a randomized, controlled trial. Diabetes. 2009;58(8):1921-8.
- Abstract Viewed: 6 times
- PDF Downloaded: 6 times