Evaluation of immune responses induced by hydroalcoholic extract of Allium ampeloprasum. L subsp iranicum in mice Allium iranicum as a powerful immune system booster
Iranian Journal of Pharmaceutical Sciences,
Vol. 18 No. 3 (2022),
1 Tir 2022
,
Page 176-182
https://doi.org/10.22037/ijps.v18.42759
Abstract
The extract of some plants can play a role in modulating the function of the human immune system. Several studies have shown that Allium species can stimulate the immune system through different cellular mechanisms. In this study the effect of the hydroalcoholic extract of Allium ampeloprasum. L subsp iranicum on the immune responses of NMRI mice was investigated. Mice were randomly divided into three groups and each group was divided into four subgroups: normal saline, levamisole (20 kg/mg), A. Iranicum (150 kg/mg), and A. Iranicum (300 kg/mg). To evaluate immune responses, body and spleen weight, white blood cell count, delayed hypersensitivity response (DTH), antibody titer (HA), and lymphocyte proliferation test were used.
A. Iranicum provoked several parameters including spleen weight, spleen cellularity, proliferation response to lipopolysaccharide (LPS) and phytohemagglutinin-A (PHA), HA titer, and DTH response. While A. Iranicum did not affect body weight and white blood cell counts. Considering the obtained results, it seems that the A. Iranicum can play an effective role in activating the immune system. To better understand the effect of the A. Iranicum on different cellular mechanisms of the immune system, further research is needed.
Keywords:
- Allium iranicum
- Cellular immune system
- Herbal extract
- Humoral immune system
- Levamisole
- NMRI mice
How to Cite
Shiehzadeh , F. ., Vakili, P. ., & Shirani , K. . (2022). Evaluation of immune responses induced by hydroalcoholic extract of Allium ampeloprasum. L subsp iranicum in mice: Allium iranicum as a powerful immune system booster. Iranian Journal of Pharmaceutical Sciences, 18(3), 176–182. https://doi.org/10.22037/ijps.v18.42759
References
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[28] Iciek M, Kwiecień I, Włodek L. Biological properties of garlic and garlic‐derived organosulfur compounds. Environ. Mol. Mutagen. (2009) 50(3): 247-265.
[29] Aryakia E, Karimi HR, Naghavi MR, Yazdanfar N, Fazeli SAS. Evaluating essential oil composition of four Allium species (Subgen. & Sect. Allium). Iranian J. Field. Crop. Sci (2018) 49(1):114- 123 .
[2] Walach H., Jonas WB, Lewith GT. The role of outcomes research in evaluating complementary and alternative medicine. Altern Ther Health Med (2002) 8(3): 88-95.
[3] Chaplin DD. Overview of the immune response. J Allergy. Clin. Immunol (2010) 125 (2 Suppl 2): S3-23.
[4] Zipfel PF. Complement and immune defense: from innate immunity to human diseases. Immunol. Lett. (2009) 126 (1-2): 1-7.
[5] Babich O, Sukhikh S, Prosekov A, Asyakina L, Ivanova S. Medicinal Plants to Strengthen Immunity during a Pandemic, Pharmaceuticals (Basel, Switzerland) (2020) 13 (10):313.
[6] Sultan MT, Butt M.S, Qayyum MM, Suleria HA. Immunity: plants as effective mediators. Crit. Rev. Food. Sci. Nutr (2014 (54(10): 1298-1308.
[7] de Vahl E, Svanberg I. Traditional uses and practices of edible cultivated Allium species (fam. Amaryllidaceae) in Sweden. Metab. Brain Dis (2022) 18 (1): 14.
[8] Miri SM, Roughani A. Allium species growing in Iran: Botany and distribution, 2019.
[9] Saeedi M, Yeganegi M, Alizadeh BB, Vasiee A, Tabatabaei Y.F. Antimicrobial effects of leek (Allium ampeloprasum l. Subsp. Iranicum) extract on some food-borne pathogens in vitro. Food Sci. Technol (2017) 14 (7): 73-82.
[10] Fatoorechi V, Rismanchi M, Nasrollahzadeh J. Effects of Persian leek (Allium ampeloprasum) on hepatic lipids and the expression of proinflammatory gene in hamsters fed a high-fat/high-cholesterol diet, Avicenna J. Phytomed (2016) 6(4): 418–424.
[11] Shirani K, Riahi-Zanjani B, Omidkhoda SN., Barangi S, Karimi G. The hematopoietic potential of methanolic and aqueous extracts of Portulaca oleracea in a phenylhydrazine model of anemia, Avicenna J. Phytomed (2023) 13(1): 85-96.
[12] Soodi M, Saeidnia S, Sharifzadeh M, Hajimehdipoor H, Dashti A, Sepand M.R, Moradi S. Satureja bachtiarica ameliorate beta-amyloid induced memory impairment, oxidative stress and cholinergic deficit in animal model of Alzheimer’s diseaseMetab. Brain Dis (2016) 31(2): 395-404.
[13] Khalaji S, Eidi M, Eidi A, Shahmohammadi P, Arab M. Hypoglycaemic effect of ethanolic extract leaves of Allium ampeloprasum L. subsp. iranicum in healthy and diabetic NMARI mice. Iran J Biological Sci (2009) 4: 9-14.
[14] Abbas MA. Analgesic effect of Allium ampeloprasum: Evidence for the involvement of beta-adrenergic system. J. Funct. Foods (2019) 57: 1-6.
[15] Ahmad W, Jantan I, Kumolosasi E, Bukhari SNA. Immunostimulatory effects of the standardized extract of Tinospora crispa on innate immune responses in Wistar Kyoto rats. Drug Des Devel Ther (2015) 9: 2961-2973
[16] Shirani K, Riahi Zanjani B, Mehri S, Razavi-Azarkhiavi K, Badiee A, Hayes AW, Giesy JP, Karimi G. miR-155 influences cell-mediated immunity in Balb/c mice treated with aflatoxin M1. Drug Chem Toxicol (2021) 44(1): 39-46.
[17] Shirani K, Riahi Zanjani B, Mehri, Mahmoudi M, Jafarian AH, Hassani VF, Giesy JP, Karimi G Immunotoxicity of aflatoxin M1: as a potent suppressor of innate and acquired immune systems in a subacute study, J Sci Food Agric (2018) 98(15): 5884-5892.
[18] Shirani K, Hassani FV, Razavi-Azarkhiavi K, Heidari S, Riahi-Zanjani B, Karimi G. Phytotrapy of cyclophosphamide-induced immunosuppression. Environ. Toxicol. Pharmacol (2015). 39 (3): 1262-1275. [19] Mirabeau TY, Samson ES. Effect of Allium cepa and Allium sativum on some immunological cells in rats, African journal of traditional, complementary, and alternative medicines: Afr. J. Tradit. Complement. Altern. Med (2012) 9(3): 374-379.
[20] Najeebullah S, Shinwari ZK, Jan SA, Khan I, Ali M. Ethno medicinal and phytochemical properties of genus Allium: A review of recent advances. Pak. J. Bot (2021) 53(1) 135-144.
[21] Mohamed EH, Baiomy AAA, Ibrahim ZS, Soliman MM. Modulatory effects of levamisole and garlic oil on the immune response of Wistar rats: Biochemical, immunohistochemical, molecular and immunological study. Mol. Med. Rep (2016) 14(3): 2755-2763.
[22] Farhadi L, Mohammadi-Motlagh HR, Seyfi P, Mostafaie A. Low concentrations of flavonoid-rich fraction of shallot extract induce delayed-type hypersensitivity and TH1 cytokine IFNγ expression in Balb/c Mice. J. Cell. Mol. Med (2014) 3(1): 16-25.
[23] Radjabian T, Hosseinpur Yektaei Z, Ghazanfari T, Nasiri Z, Fotovvat M. The immunoregulatory effects of four Allium species on macrophages and lymphocytes viability. Immunoregulation (2019) 2(1): 25-34.
[24] Hanieh H, Narabara K, Piao M, Gerile C, Abe A, Kondo Y. Modulatory effects of two levels of dietary Alliums on immune response and certain immunological variables, following immunization, in White Leghorn chickens. Anim. Sci. J (2010) 81(6): 673-680.
[25] Chowdhury RR and Ghosh SK. Phytol-derived novel isoprenoid immunostimulants. Front. Immunol. (2012) 3: 49:1-11.
[26] Aachoui Y, Chowdhury R.R, Fitch R.W, Ghosh S.K. Molecular signatures of phytol-derived immunostimulants in the context of chemokine–cytokine microenvironment and enhanced immune response. Cell. Immunol. (2011) 271(2): 227-238.
[27] Arsenijevic D, Stojanovic B, Milovanovic J, Arsenijevic A, Simic M, Pergal M, Kodranov I, Cvetkovic O, Vojvodic D. Ristanovic E. Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin A-induced hepatitis. Nutrients (2021) 13(3): 1022.
[28] Iciek M, Kwiecień I, Włodek L. Biological properties of garlic and garlic‐derived organosulfur compounds. Environ. Mol. Mutagen. (2009) 50(3): 247-265.
[29] Aryakia E, Karimi HR, Naghavi MR, Yazdanfar N, Fazeli SAS. Evaluating essential oil composition of four Allium species (Subgen. & Sect. Allium). Iranian J. Field. Crop. Sci (2018) 49(1):114- 123 .
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