Study of Aptamer-Attached Juglone in Different pH Ranges and Ionic Concentrations of Buffers Electrochemical aptamer-based sensors
Iranian Journal of Pharmaceutical Sciences,
Vol. 8 No. 2 (2012),
1 April 2012
,
Page 121-128
https://doi.org/10.22037/ijps.v8.40962
Abstract
Electrochemical aptamer-based sensors attract a lot of interest as useful methods because of their low cost, accuracy, sensitivity, and simplicity. An electro-active redox molecule comprises the main part of the electrochemical-based sensors. Ferrocene is one of the most popular redox molecule used in biosensor fabrication. But, instability of ferrocenium ion in strong nucleophilic reagents and chloride containing
solutions is one of the main problems of this redox molecule. In this study, Juglone is used as an effective quinone redox molecule for aptasensor designing in different pH ranges and different concentrations of chloride ion. The voltammetric studies showed that the electrochemical response of sensor increased by raising the buffer ionic concentration and the sensor accuracy in 7.0 to 8.0 pH range as well. According
to the findings, Juglone could be used as an effective redox molecule in high concentrations of chloride containing solutions in the 7.0 pH.
Keywords:
- Aptamer
- Aptasensor
- Juglone
- pH
- Redox
How to Cite
Mehdi Saberian, Davoud Asgari, Yadollah Omidi, & Hossein Hamzeiy. (2012). Study of Aptamer-Attached Juglone in Different pH Ranges and Ionic Concentrations of Buffers: Electrochemical aptamer-based sensors. Iranian Journal of Pharmaceutical Sciences, 8(2), 121–128. https://doi.org/10.22037/ijps.v8.40962
References
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[34] Ellington AD, Szostak JW. In vitro selection of RNA molecules that bind specific ligands. Nat 1990; 346: 818-22.
[35] Ellington AD, Szostak JW. Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures. Nature 1992;355: 850-2.
[36] Hermann T, Patel DJ. Adaptive recognition by nucleic acid aptamers. Sci 2000; 287: 820-5.
[37] Tuerk C, Gold L. Systemic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 1990;249:505-10.
[38] Toulme JJ, Daguer JP, Dausse E. Aptamers:ligands for all reasons. In: Mascini M, (editor).Aptamers in bioanalysis. New Jersey: John Willy & Sons INC 2009; pp. 3-30.
[39] Berg JM, Tymoczko JL, Stryer L. Biochemistry.New York: W.H. Freeman and Company 2002;117-42.
[40] Strehlitz B, Stoltenburg G. SELEX and its recent optimisations. In: Mascini M, (editor). Aptamers in bioanalysis. New Jersey: John Wiley & Sons,INC 2009;31-61.
[41] Stoltenburg R, Reinemann C, Strehlitz B. SELEXA (r)evolutionary method to generate high-affinity nucleic acid ligands. Biomol Eng 2007; 24: 381-403.
[2] Neyraud E, Bult JHF, Dransfield E. Continuous analysis of parotid saliva during resting and shortduration simulated chewing. Arch Oral Biol 2009; 54: 449-56.
[3] Koff SG, Paquette EL, Cullen J, Gancarczyk KK, Tucciarone PR, Schenkman NS. Comparison between lemonade and potassium citrate and impact on urine pH and 24-hour urine parameters in patients with kidney stone formation. Urol 2007; 69: 1013-6.
[4] Eisner BH, Porten SP, Bechis SK, Stoller ML.Diabetic kidney stone formers excrete more oxalate and have lower urine pH than nondiabetic stone formers. J Urol 2010; 183: 2244-8.
[5] Chang IH, Lee YT, Lee DM, Kim TH, Myung SC, Kim YS. Metabolic syndrome, urine pH, and timedependent risk of nephrolithiasis in Korean men without hypertension and diabetes. Urol 2011; 78: 753-8.
[6] Guyton AC, Hall JE. Textbook of medical physiology. Philadelphia: W.B. Saunders Company, 2000; pp. 738-53.
[7] Song S, Wang L, Li J, Fan C, Zhao J. Aptamerbased biosensors. TrAC-Trend Anal Chem 2008; 27: 108-17.
[8] Lee JO, So HM, Jeon EK, Chang H, Won K, Kim YH. Aptamers as molecular recognition elements for electrical nanobiosensors. Analyt Bioanal Chem 2008; 390: 1023-32.
[9] Tombelli S, Minunni M, Mascini M. Analytical applications of aptamers. Biosens Bioelectro 2005; 20: 2424-34.
[10] Velasco-Garcia MN. Optical biosensors for probing at the cellular level: a review of recent progress and future prospects. Semin Cell Dev Biol 2009; 20: 27-33.
[11] Electrochemical biosensors: recommended definitions and classification (Technical Report).Pure Appl Chem 1999; 71: 2332-48.
[12] Wang J. Electrochemical biosensors: towards point-of-care cancer diagnostics. Biosens Bioelectron 2006; 21: 1887-92.
[13] Odenthal KJ, Gooding JJ. An introduction to electrochemical DNA biosensors. Analyst 2007; 132: 603-10.
[14] Xiao Y, Lai RY, Plaxco KW. Preparation of electrode-immobilized, redox-modified oligonucleotides for electrochemical DNA and aptamer-based sensing. Nat Protoc 2007; 2: 2875-80.
[15] Ferapontova EE, Olsen EM, Gothelf KV. An RNA aptamer-based electrochemical biosensor for detection of theophylline in serum. J Am Chem Soc 2008; 130: 4256-8.
[16] Baker BR, Lai RY, Wood MS, Doctor EH, Heeger AJ, Plaxco KW. An electronic, aptamer-based small-molecule sensor for the rapid, label-free detection of cocaine in adulterated samples and biological fluids. J Am Chem Soc 2006; 128:3138-9.
[17] Wang X, Dong P, Yun W, Xu Y, He P, Fang Y. A solid-state electrochemiluminescence biosensing switch for detection of thrombin based on ferrocene-labeled molecular beacon aptamer.Biosens Bioelectron 2009; 24: 3288-92.
[18] Li Y, Qi H, Peng Y, Gao Q, Zhang C. Electrogenerated chemiluminescence aptamer-based method for the determination of thrombin incorporating quenching of tris(2,2'-bipyridine)ruthenium by
ferrocene. Electrochem Commun 2008; 10: 1322-5.
[19] Hurvois JP, Moinet C. Reactivity of ferrocenium cations with molecular oxygen in polar organic solvents: decomposition, redox reactions and stabilization. J Organomet Chem 2005; 690: 1829-39.
[20] Prins R, Korswagen AR, Kortbeek AGTG. Decomposition of the ferricenium cation by nucleophilic reagents. J Organomet Chem 1972; 39: 335-44.
[21] Chatterjee M, Rokita SE. The role of a quinone methide in the sequence specific alkylation of DNA. J Am Chem Soc 1994; 116: 1690-7.
[22] Ngameni E, Tonle IK, Nanseu CP, Wandji R.Voltammetry study of 2-Hydroxy-3-isopropenyl-1,4-naphthoquinone using a carbone paste electrode. Electroanal 2000; 12: 847-52.
[23] Jahan D, Raoof B, Golabi SM. Electrochemical properties of carbon-paste electrodes spiked with some 1,4-naphtoquinone derivatives. Bull Chem Soc Jpn 1995; 68: 2253-61.
[24] Paulsen MT, Ljungman M. The natural toxin juglone causes degradation of p53 and induces rapid H2AX phosphorylation and cell death in human fibroblasts. Toxicol Appl Pharm 2005;209: 1-9.
[25] March G, Nool V, Piro B, Reisberg S, Pham MC.Nanometric layers for direct, signal-on, selective, and sensitive electrochemical detection of oligonucleotides hybridization. J Am Chem Soc 2008;130: 15752-3.
[26] Win MN, Klein JS, Smolke CD. Codeine-binding RNA aptamers and rapid determination of their binding constants using a direct coupling surface plasmon resonance assay. Nucleic Acids Res 2006;
34: 5670-82.
[27] Online Source: http://psiweb.unl.edu/cahoon/files/phosphate%20buffer.pdf. Available at 1/28/2012.
[28] Online Source: http://en.wikipedia.org/wiki /Henderson%E2%80%93Hasselbalch_equation.Available at 1/28/2012.
[29] Po HN, Senozan NM. The Henderson-Hasselbalch equation: Its history and limitations.J Chem Educ 2001; 78: 1499-503.
[30] El-Deab MS, Ohsaka T. Molecular-level design of binary self-assembled monolayers on polycrystalline gold electrodes. Electrochim Acta 2004; 49: 2189-94.
[31] Merrill DR, Stefan IC, Scherson DA, Mortimer JT. Electrochemistry of gold in aqueous sulfuric acid solutions under neural stimulation conditions.J Electrochem Soc 2005; 152: 212-21.
[32] Wink T, Van-Zuilen SJ, Bult A, Van-Bennekom WP. Self-assembled monolayers for biosensors. Analyst 1997; 122; 43-50.
[33] Saberian M, Hamzeiy H, Aghanejad A, Asgari D.Aptamer-based nanosensors: juglone as an attached-redox molecule for detection of small molecules. Bioimpacts 2011; 1: 31-6.
[34] Ellington AD, Szostak JW. In vitro selection of RNA molecules that bind specific ligands. Nat 1990; 346: 818-22.
[35] Ellington AD, Szostak JW. Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures. Nature 1992;355: 850-2.
[36] Hermann T, Patel DJ. Adaptive recognition by nucleic acid aptamers. Sci 2000; 287: 820-5.
[37] Tuerk C, Gold L. Systemic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 1990;249:505-10.
[38] Toulme JJ, Daguer JP, Dausse E. Aptamers:ligands for all reasons. In: Mascini M, (editor).Aptamers in bioanalysis. New Jersey: John Willy & Sons INC 2009; pp. 3-30.
[39] Berg JM, Tymoczko JL, Stryer L. Biochemistry.New York: W.H. Freeman and Company 2002;117-42.
[40] Strehlitz B, Stoltenburg G. SELEX and its recent optimisations. In: Mascini M, (editor). Aptamers in bioanalysis. New Jersey: John Wiley & Sons,INC 2009;31-61.
[41] Stoltenburg R, Reinemann C, Strehlitz B. SELEXA (r)evolutionary method to generate high-affinity nucleic acid ligands. Biomol Eng 2007; 24: 381-403.
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