Simple and Sensitive High Performance Liquid Chromatographic (HPLC) Method for the Determination of the Apigenin from Dried Powder of Cosmos Bipinnatus, Apium Graveolens and Petroselinum Crispum Determination of Apigenin in dried powder of plants
Iranian Journal of Pharmaceutical Sciences,
Vol. 12 No. 2 (2016),
1 April 2016
,
Page 21-32
https://doi.org/10.22037/ijps.v12.40774
Abstract
Apigenin is best known as an irreversible inhibitor of monoamine oxidase (MAO), an intracellular enzyme associated with the outer membrane of mitochondria. The purpose of this study is to establish a reliable and quick method for the assignment of apigenin in Cosmos bipinnatus, Apium graveolens, and Petroselinum crispum by HPLC. A rapid and sensitive HPLC method has been developed for determination of apigenin. Mobile phase was composed water-acetonitrile (55:45 v/v) with a flow rate of 1 ml/min and the eluted peaks were detected by a UV detector set at wavelength of 340 nm. The method was validated in the range of apigenin concentrations from 0.01 to 500 μg/ml and the limits of detection (LOD) and quantitation (LOQ) of the method were 0.005 and 0.01 μg/ml, respectively. The average recovery throughout the linear concentration range was 97.28 percent and the averages for within-run and between-run accuracy values were 99.32 and 96.79 respectively. The method is quick, simple, sensitive, and precise for the screen, assignment, and evaluation of apigenin in plants by HPLC.
Keywords:
- Apigenin
- HPLC
- Cosmos bipinnatus
- Apium graveolens
- Petroselinum crispum
How to Cite
Ali Sharafi, Soroush Bijani, Hamidreza Kheiri-Manjili, Alireza Ahmadnia, & Hossein Danafar. (2016). Simple and Sensitive High Performance Liquid Chromatographic (HPLC) Method for the Determination of the Apigenin from Dried Powder of Cosmos Bipinnatus, Apium Graveolens and Petroselinum Crispum: Determination of Apigenin in dried powder of plants. Iranian Journal of Pharmaceutical Sciences, 12(2), 21–32. https://doi.org/10.22037/ijps.v12.40774
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[28] Danafar H. Method validation of colonidine in human plasma by LC-MS. Pharm Biomed Res (2015) 1(4): 48-58
[29] Svenningsen A.B, Madsen K.D, Liljefors T. Biflavones from Rhus species with affinity for the GABA A/benzodiazepine receptor. Journal of ethnopharmacology (2006) 103: 276-280.
[30] Wang C.-L, Li H.-Q, Meng W.-D. Trifluoromethylation of flavonoids and anti-tumor activity of the trifluoromethylated flavonoid derivatives. Bioorganic & medicinal chemistry letters (2005) 15: 4456-4458.
[31] Danafar H. A quick and easy high performance liquid chromatography method for evaluation of cefixime in human plasma. Pharm Biomed Res (2015) 1: 29-39.
[32] Danafar H, Hamidi M. Pharmacokinetics and Bioequivalence of Methotrexate in Human Plasma Studied by Liquid Chromatography-Mass Spectrometry (LC-MS). Jundishapur J Nat Pharm Prod. 11(2):e36758.
[33] Danafar H. Simple and sensitive high performance liquid chromatographic (HPLC) method for the determination of the selegiline in human plasma. Cogent Medicine 2016 3: 1179244.
[2] Danafar H, Sharafi A, Kheiri Manjili H, Andalib S. Sulforaphane delivery using mPEG–PCL co-polymer nanoparticles to breast cancer cells. Pharmaceutical development and technology. (2016) 1-10.
[3] Porbarkhordari E, Foladsaz K, Hoseini S.H, Danafar H, Kheiri Manjili H.R. Ramazani A. The Hypoglycemic Effects of an Ethanol Extract of Peganum harmala in Streptozotocin-Induced Diabetic Rats. Iranian Journal of Pharmaceutical Sciences. (2014) 10: 47-54.
[4] Škerget M, Kotnik P. Hadolin M, Hraš A.R. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food chemistry. (2005) 89: 191-198.
[5] Danafar H, Hamidi M. Liquid chromatography–tandem mass spectrometry (LC-MS) method for the assignment of enalapril and enalaprilat in human plasma. Pharm Biomed Res. (2015) 1: 47-58
[6] Hougee S, Sanders A, Faber J. Decreased pro-inflammatory cytokine production by LPS-stimulated PBMC upon in vitro incubation with the flavonoids apigenin, luteolin or chrysin, due to selective elimination of monocytes/macrophages. Biochemical pharmacology . (2005) 69: 241-248.
[7] Kim D.-I, Kim C.-H. Regulation of IGF-I production and proliferation of human leiomyomal smooth muscle cells by Scutellaria barbata D. Don in vitro: isolation of flavonoids of apigenin and luteolin as acting compounds. Toxicology and applied pharmacology. (2005) 205: 213-224.
[8] Martini N, Katerere D, Eloff J. Biological activity of five antibacterial flavonoids from Combretum erythrophyllum (Combretaceae). Journal of ethnopharmacology (2004) 93: 207-212.
[9] Danafar H, Hamidi, M. A quick and sensitive liquid chromatography–tandem mass spectrometry (LC-MS) method for the determination of enalapril and enalaprilat in human plasma: Application to a Bioequivalence Study. Iranian journal of pharmaceutical sciences. (2014) 10(3): 21-34.
[10] Danafar H, Hamidi M.LC-MS Method for Studying the Pharmacokinetics and Bioequivalence of Clonidine Hydrochloride in Healthy Male Volunteers. Avicenna J Med Biotech (2016) 8(2): 91-98
[11] Wang C.-L, Li H.-Q, Trifluoromethylation of flavonoids and anti-tumor activity of the trifluoromethylated flavonoid derivatives. Bioorganic & medicinal chemistry letters (2005)15: 4456-4458.
[12] Abate A, Yang G, Wong R.J.. Apigenin decreases hemin-mediated heme oxygenase-1 induction. Free Radical Biology and Medicine (2005) 39: 711-718.
[13] Choi E.J, Kim G.-H. Apigenin induces apoptosis through a mitochondria/caspase-pathway in human breast cancer MDA-MB-453 cells. Journal of clinical biochemistry and nutrition (2009) 44: 260-265.
[14] Danafar H, Hamidi M. A Rapid and Sensitive LC–MS Method for Determination of ezetimibe Concentration in Human Plasma: Application to a Bioequivalence Study. Chromatographia (2013) 76: 1667-1675.
[15] Hirano T, Higa S, Arimitsu J. Luteolin J. a flavonoid, inhibits AP-1 activation by basophils. Biochemical and biophysical research communications ( 2006) 340: 1-7.
[16] Hougee S, Sanders A. Decreased pro-inflammatory cytokine production by LPS-stimulated PBMC upon in vitro incubation with the flavonoids apigenin, luteolin or chrysin, due to selective elimination of monocytes/macrophages. Biochemical pharmacology (2005) 69: 241-248.
[17] Danafar H, Hamidi M. Pharmacokinetics and Bioequivalence Study of Amlodipine and Atorvastatin in Healthy Male Volunteers by LC-MS. Pharmaceutical Sciences (2015) 21: 167-174
[18] Jang I.-C, Park J.-H, Park E. Antioxidative and antigenotoxic activity of extracts from cosmos (Cosmos bipinnatus) flowers. Plant foods for human nutrition (2008) 63: 205-210.
[19] Khan T.H, Sultana S. Apigenin induces apoptosis in Hep G2 cells: possible role of TNF-α and IFN-γ. Toxicology (2006) 217: 206-212.
[20] Danafar H, Hamidi M. Simple and sensitive high performance liquid chromatographic method for the simultaneous quantitation of the phenylalanine in human plasma. Pharm Biomed Res (2015) 1: 12-20
[21] Kim, D.-I., Lee, T.-K., Lim, I.-S. Regulation of IGF-I production and proliferation of human leiomyomal smooth muscle cells by Scutellaria barbata D. Don in vitro: isolation of flavonoids of apigenin and luteolin as acting compounds. Toxicology and applied pharmacology (2005) 205: 213-224.
[22] Kuo M.-L, Lee K.-C, Lin J.-K. Genotoxicities of nitropyrenes and their modulation by apigenin, tannic acid, ellagic acid and indole-3-carbinol in the Salmonella and CHO systems. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis (1992) 270: 87-95.
[23] Li L.-P, Jiang H.-D. Determination and assay validation of luteolin and apigenin in human urine after oral administration of tablet of Chrysanthemum morifolium extract by HPLC. Journal of pharmaceutical and biomedical analysis (2006) 41: 261-265.
[24] Martini N, Katerere D, Eloff J. Biological activity of five antibacterial flavonoids from Combretum erythrophyllum (Combretaceae). Journal of ethnopharmacology (2004) 93: 207-212.
[25] Rithidech K.N, Tungjai M, Whorton E.B. Protective effect of apigenin on radiation-induced chromosomal damage in human lymphocytes. Mutation Research/Genetic Toxicology and Environmental Mutagenesis (2005) 585: 96-104.
[26] Danafar H, Hamidi M. Simple and Sensitive High-Performance Liquid Chromatography (HPLC) Method with UV Detection for Mycophenolic Acid Assay in Human Plasma. Application to a Bioequivalence Study. Adv Pharm Bull (2015) 5: 563-568.
[27] Sohn S.-H, Yun B.-S, Kim S.-Y. Anti-inflammatory activity of the active components from the roots of Cosmos bipinnatus in lipopolysaccharide-stimulated RAW 264.7 macrophages. Natural product research (2013) 27: 1037-1040.
[28] Danafar H. Method validation of colonidine in human plasma by LC-MS. Pharm Biomed Res (2015) 1(4): 48-58
[29] Svenningsen A.B, Madsen K.D, Liljefors T. Biflavones from Rhus species with affinity for the GABA A/benzodiazepine receptor. Journal of ethnopharmacology (2006) 103: 276-280.
[30] Wang C.-L, Li H.-Q, Meng W.-D. Trifluoromethylation of flavonoids and anti-tumor activity of the trifluoromethylated flavonoid derivatives. Bioorganic & medicinal chemistry letters (2005) 15: 4456-4458.
[31] Danafar H. A quick and easy high performance liquid chromatography method for evaluation of cefixime in human plasma. Pharm Biomed Res (2015) 1: 29-39.
[32] Danafar H, Hamidi M. Pharmacokinetics and Bioequivalence of Methotrexate in Human Plasma Studied by Liquid Chromatography-Mass Spectrometry (LC-MS). Jundishapur J Nat Pharm Prod. 11(2):e36758.
[33] Danafar H. Simple and sensitive high performance liquid chromatographic (HPLC) method for the determination of the selegiline in human plasma. Cogent Medicine 2016 3: 1179244.
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