Thrombolytic, Membrane stabilizing, Antidiarrhoeal, and Antimicrobial Properties of Bioactive Compounds Isolated from leaves of Sesbania grandiflora Naturally Growing in Bangladesh Properties of Bioactive Compounds Isolated from leaves of Sesbania grandiflora
Iranian Journal of Pharmaceutical Sciences,
卷 12 编号 3 (2016),
1 July 2016
,
第 31-46 页
https://doi.org/10.22037/ijps.v12.40720
摘要
Sesbania grandiflora leaves are extensively used locally since ancient time as in traditional systems. Based on mainly folkloric use, we investigated to know the potential of Sesbania grandiflora leaves extracts through biological works in vivo and in vitro. The ethanolic extract of S. grandiflora leaves was partitioned into ethyl acetate soluble fraction (ESF), petroleum ether soluble fraction (PSF), carbon tetrachloride soluble fraction (CTSF), chloroform soluble fraction (CSF), and aqueous soluble fraction (AQSF). The extracts were evaluated for their thrombolytic, membrane stabilizing, antimicrobial and antidiarrhoeal activities and the results were compared with standard drugs; streptokinase for thrombolytic, acetyl salicylic acid for anti-inflammatory, kanamycin for antimicrobial activities and loperamide against diarrhoea. In thrombolytic investigation, among all partitionates, the ESF showed highest % of clot lysis (59.57%) as compared to (69.23%) and (3.07%) exhibited by the standard streptokinase and water. In case of membrane stabilizing study, ESF also significantly inhibited the haemolysis of human erythrocyte membrane both induced by hypotonic solution (64.30 ± 0.64%) and by heat (57.21 ± 0.69%), respectively as compared to (70.12 ± 26%) and (73.90 ± 0.29%) demonstrated by acetyl salicylic acid. In the antidiarrhoeal assay, the ethanolic extract (EE) inhibited the mean number of defecation by 24.97% and 41.05% at 200 mg/kg and 400 mg/kg body weight, respectively. In antibacterial activity, it was exhibited by the extracts being comparatively more prominent on the gram negative bacteria than the gram positive bacteria. Our study revealed that satisfactory amount of flavonoid and tannin presence showed a significant and positive correlation between total phenolic compound contents with pharmacological activities of S. grandiflora.
- antimicrobial and antidiarrhoeal activities
- membrane stabilizing
- Sesbania grandiflora
- thrombolytic
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参考
[2] Zengin G, Aktumsek A, Guler GO, Cakmak YS, Kan Y. Composition of essential oil and antioxidant capacity of Centaurea drabifolia Sm. subsp. Detonsa (Bornm.) Wagenitz, endemic to Turkey. Nat. Prod. Res. (2012) 26(1): 1–10.
[3] Zhang YJ, Tanaka T, Iwamoto Y, Yang CR, Kouno I. Novel sesquiterpenoids from the roots of Phyllanthus emblica. J. Nat. Prod. (2001) 64(7):870- 873.
[4] Anonymous. The Wealth of India. A Dictionary of Raw Materials and Industrial Products, Raw Materials, Publications and Information Directorate, New Delhi (1972) 275 -299.
[5] Gupta C, Amar P, Ramesh G, Uniyal C, Kumari A. Antimicrobial activity of some herbal oils against common food-borne pathogens. African J. Microbial. Research (2008) 2(4): 258-261.
[6] Narayan AK, Kolammal. Pharmacognosy of Ayurvedic Drugs, Kerala, Published by Department of Pharmacognosy, University of Kerala, Trivandrum (1964), Series 1: 5-8.
[7] Siddhinandan M. Ayurvedic Rasa Sastra 7th edition (Published by Choukhambh Orientalia, Varanasi (1997), 453.
[8] Gopalan C, Ramasastri BV, Balasubramanian SC. Nutritive Value of Indian Foods National Institute of Nutrition (Indian council of Medical Research) Hyderabad, India (2007).
[9] Ghani A. Medicinal Plants of Bangladesh with chemical constituents and uses. 2nd edition, Asiatic Society of Bangladesh (2002). 5 old Secretariate road, Nimtali, Dhaka, Bangladesh.
[10] Goun E, Cunningham G, Chu D, Nguyen C, Miles D. Antibacterial and antifungal activity of Indonesian ethnomedical plants. Fitoterapia (2003) 76(5):592–596.
[11] Ramesh T, Begum VH. Effect of Sesbania grandiflora on lung antioxidant defense system in cigarette smoke exposed rats. Int. J. Biol. Chem (2007) 1(4):141–148.
[12] Ramesh T, Begum VH. Protective effect of Sesbania grandiflora against cigarettes smoke-induced oxidative damage in rats. J. Med. Food (2008) 11(2):369–375.
[13] Doddola S, Pasupulati H., Koganti B., Prasad K.V.S.R.G. Evaluation of Sesbania grandiflora for antiurolithiatic and antioxidant properties. J. Nat. Med (2008) 62(3):300–307.
[14] Kasture VS, Deshmukh VK, Chopde CT. Anxiolytic and anticonvulsive activity of Sesbania grandiflora leaves in experimental animals. Phytother Res (2002) 16(5):455-60
[15] Pari L, Uma A. Protective effect of Sesbania grandiflora against erythromycin estolate-induces hepatotoxicity. Therapie (2003) 58(5):439–443.
[16] Fingel E. Cathartics and laxatives. In The Pharmacological Basis of Therapeutics. L. S. Goodman and A. Gilmani, editors. Macmillan Publishing Co., New York. (1970) 1020- 1024.
[17] Kupchan SM, Gray AH, Grove MD. J Med Chemistry (1967) 10:337–340
[18] Sofowara A. Medicinal Plants and Traditional Medicinal in Africa. 2nd Ed. Sunshine House, Ibadan, Nigeria: Spectrum Books Ltd (1993). Screening Plants for Bioactive Agents; pp. 134–156.
[19] Kumaran A, Karunakaran RJ. In vitro antioxidant properties of methanol extracts of five Phyllanthus species from India LWT-Food Sci Technol (2007) 40: 344–352
[20] Kim DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem (2003) 81:321–326.
[21] Daginawala H.F, Prasad S, Kashyap RS, Deopujari JW, Purothi HJ. Development of an in vitro model to study clot lysis activity of thrombolytic drugs. Thrombosis J (2006) 2(9): 1-4
[22] Omale J, Okafor PN. Comparative antioxidant capacity, membrane stabilization, polyphenol composition and cytotoxicity of the leaf and stem of Cissus multistriata. African Journal of Biotechnology (2008) 7(17):3129-3133
[23] Shinde UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, Sarsf MN. Membrane stabilization activity- a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia (1989) 70(3): 251–257.
[24] Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain. (1983) 16:109
[25] Zubair KL, Farhina RL, Mir AM, Md SH. Antidiarrhoeal Activity and Total Tannin Content of Ethanolic Leaf Extract of Codiaeum variegatum. Dhaka Univ. J. Pharm. Science (2015) 14(1): 87-90.
[26] Shoba FG, Thomas M. Study of antidiarrheal activity of four medicinal plants in castor oil-induced diarrhea. J. Ethnopharmacol (2001) 76(1): 73-76.
[27] Hilaly JE, Israili ZH, Lyoussi B. Acute and chronic toxicological studies of Ajugaiva in experimental animals. J Ethnopharmacol (2004) 9(1):43–50.
[28] Ali-Shtayeh MS, Al-Nuri MA, Yaghmour RM, Faidi YR. Antimicrobial activity of Micromeria nervosa from the Palestine area. J. Ethnopharm. (1997) 58(3):143–147
[29] Cos P, Vlietinck AJ, Vanden Berghe D, Maes L. Anti-infective potential of natural products: How to develop a stronger in vitro ‘proof-of-concept’. J Ethnopharmacol (2006) 106(3):290–302.
[30] Sainath RS, Prathiba J, Malathi R. Antimicrobial properties of the stem bark of Saraca indica (Caesalpiniaceae). Eur Rev MedPharmacol Sci. (2009) 13: 371-374.
[31] Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods, Journal of Food and Drug Analysis. (2002) 10(3): 178-182
[32] Okoli CO, Akah PA, Onuoha NJ, Okoye TC, Nwoye AC, Nworu CS. Acanthus montanus. An experimental evaluation of the antimicrobial, anti-inflammatory and immunological properties of a traditional remedy for furuncles. BMC Complement Altern Medicine (2008) 8:27. Doi: 10.1186/1472-6882-8-27
[33] Nikhil S, Amit B. "A history of streptokinase use in acute myocardial infarction". Texas Heart Inst Journal (2007) 34(3): 318–327.
[34] Meneveau N, Schiele F, Vuillemenot A. "Streptokinase vs alteplase in massive pulmonary embolism. A randomized trial assessing right heart haemodynamics and pulmonary vascular obstruction". Eur. Heart J. (1997) 18(7): 1141–1148.
[35] Mundada L, Prorok M. "Structure-Function Analysis of Streptokinase Amino Terminus". Journal of Biological Chemistry (2003) 278(3): 24421–24427.
[36] Pantzar M, Ljungh A, Wadström T. Plasminogen binding and activation at the surface of Helicobacter pylori CCUG 17874. Infect Immun. (1998) 66(10):4976–4980.
[37] Igbinosa OO, Igbinosa EO, Aiyegoro OA. Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn). Afr. J. Pharmacy Pharmacol (2009) 3(2): 058-062.
[38] Meyer H. Ueber den wirksamen Bestandtheil des Ricinusöls. [On the active component of castor oil) Arch Exp Path Pharmak (1890) 28:145–152. German.
[39] Watson WC, Gordon RS, Jr. Studies on the digestion, absorption and metabolism of castor oil. Biochem Pharmacol (1962) 11:229 –236.
[40] Burdock GA, Carabin IG, Griffiths JC. Toxicology and pharmacology of sodium ricinoleate. Food Chem Toxicol (2006) 44(10):1689–1698.
[41] Ahmadiani A, Hosseiny J, Semnanian S, Javan M, Saeedi F, Kamalinejad M. Antinociceptive and anti-inflammatory effects of Elaeagnus angustifolia fruit extract. J Ethnopharmacol (2000) 72(1-2):287–292
[42] Zubair KL, Jalal U, Azima SJ, Md H A, Md. NI, Shah AK. Antimicrobial, Thrombolytic, Membrane Stabilizing Activities and Total Flavonoid Content of Various Partitionates of Aerial Parts of Eclipta alba (L.) Hassk. Dhaka Univ. J. Pharm. Science (2015) 14(2): 207-213.
[43] Cushnie TP, Lamb AJ. "Recent advances in understanding the antibacterial properties of flavonoids". In
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