New Elaborated Technique for Isolation and Purification of Limonene from Orange Oil Isolation of limonene from orange oil
Iranian Journal of Pharmaceutical Sciences,
Vol. 2 No. 2 (2006),
1 April 2006
,
Page 87-90
https://doi.org/10.22037/ijps.v2.39555
Abstract
Essential oil of the orange peel which was extracted by hydrodistillation method showed 50 different mono- and sesquiterpenes on GC-MS analysis, in which
limonene had the highest percentage (92.4%). Due to various important uses of the compound, a method was developed for isolation and purification of limonene. In this method, for extra purification, a fractional distillation on Vigreux column under 100 mm Hg vacuum was used which resulted in 96.7% purity for limonene.
Subsequent chromatography by two different packages of silica gel and active charcoal columns resulted in 99.4% and 99.9 % purity of limonene, respectively.
- Column chromatography
- Fractional distillation
- Limonene
- Orange oil
- Vigreux
How to Cite
References
[2] Tyler VE, Brady LR, Robbers JE. Pharmacognosy. 9th ed. Philadelphia: Lea and Febiger, 1988; p. 111.
[3] The Merck index: an encyclopedia of chemicals, drugs and biologicals. 13th ed., New Jersey: Merck & Co., Inc.; 2001; p. 5515.
[4] Topham EJ, Wakelin SH. D-Limonene contact dermatitis from hand cleansers. Contact Dermat 2004, 49;108-9.
[5] Toro-Arreola SD, Flores-Torales E, Torres-Lozano C, Toro-Arreola AD, Tostado-Pelayo K, Guadalupe RDM, Daneri-Navarro A. Effect of DLimonene on immune response in BALB/c mice with lymphoma. Internat Immunopharmacol 2005, 5; 829-38.
[6] Elegbede JA, Flores R, Wang RC. Perillyl alcohol and peril aldehyde induced cell cycle arrest and cell death in BroTo and A549 cells cultured in vitro. Life Sci 2003, 73: 2831-40.
[7] Uedo N, Tatsuta M, Iishi H, Baba M, Sakai N, Yano H, Otani T. Inhibition by d-limonene of gastric carcinogenesis induced by N-methyl-N´- nitro-N-nitroso guanidine in Wistar rats. Cancer Lett 1999, 137: 131-6.
[8] Duke JA, Beckstrom-Sternberg SM. Hand book of medicinal mints (aromathematics) phytochemicals and biological activites. Washington: CRC press. 2001; p. 402.
[9] Dugo G; Giacomo AD. Citrus, the genus Citrus. London: Tylor and Francis, 2002; pp. 391-401.
[10] Furniss BS, Hannaford AJ, Smith WGP. Vogel’s, text book of practical organic chemistry. 15th ed., New York: Longman Scientific and Technical,
1989; pp.169-86.
[11] Pavia DL, Lampman GM, Kriz GS, Engel RG. Introduction to organic laboratory techniques. Philadelphia: Saunders, 1996; pp. 96-107.
[12] Wagner H, Bladt S. Plant drug analysis: a thin layer chromatography atlas. 2nd ed., Berlin: Springer, 1996; pp. 182-364.
[13] Adams RP. Identification of essential oil components by gas chromatography mass spectroscopy. Illinois: Allured publishing corporation, 1995; pp. 70-255.
- Abstract Viewed: 336 times
- IJPS_Volume 2_Issue 2_Pages 87-90 Downloaded: 265 times