Chemical Composition, Antiradical Capacity and Antimicrobial Activity of Hydroalcoholic Extracts of Ficus johannis Boiss
Archives of Advances in Biosciences,
Vol. 15 No. 1 (2024),
24 January 2024
,
Page 1-8
https://doi.org/10.22037/aab.v15i1.43128
Abstract
Introduction: Ficus johannis Boiss. is a shrubby species in the family Moraceae, exclusively found in Iran, Afghanistan, and Pakistan. This research studies the total phenolic content, flavonoid concentrations, antioxidant capacity, and antimicrobial activity of this biologically unknown species.
Materials and Methods: The Leaves and fruits of the plant were gathered from the northern slope of Taftan Mountain in Sistan and Baluchestan, in the summer of 2022. After washing, drying, grinding, and obtaining hydroalcoholic extracts of the fruits and leaves, quantifying the phenolic and flavonoid concentrations was done spectrophotometrically utilizing the Folin-Ciocalteu and aluminum chloride colorimetric techniques, respectively. The anti-radical activity of the organs was assayed via the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) protocol and their antimicrobial effects were assessed using the broth microdilution and streak plate techniques against six bacterial, and three fungal pathogens including Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus epidermidis, Streptococcus pyogenes, Bacillus cereus, Aspergillus fumigatus, Fusarium oxysporum, and Candida albicans.
Results: The phenolic compounds were found to be evenly distributed in both leaf and fruit extracts (around 43 mg GAE/g dry extract) while flavonoids were detected in significantly higher concentrations in the fruit extract (14.62 vs 9.57 mg QrE/g dry extract). The leaf extract was better at scavenging free radicals compared to the fruit extract (IC50 93.79 vs 239.62 μg/ml, respectively), while both extracts showed higher IC50 values than the positive control. The leaf extract showed better inhibitory effects on the tested microorganisms compared to the fruit extract. The leaf extract was effective against all tested bacteria, whereas, among the investigated fungi, only Aspergillus fumigatus was vulnerable to it. Conversely, the fruit extract was able to prevent the growth of all investigated fungal strains, but only two bacterial strains (Streptococcus pyogenes and Klebsiella pneumonia) were affected by it. Moreover, both lea and fruit extracts showed the best antimicrobial activity against Aspergillus fumigatus with MIC values of 128 and 512, repectively.
Conclusion: The Ficus johannis fruits and leaves are great sources of phenolic and flavonoid compounds with moderate anti-radical capacity. Moreover, the fruit extract mainly contains antifungal ingredients while the leaf extract chiefly includes antibacterial agents.
- Anti-bacterial agents
- Antifungal agents
- Antioxidants
- Ficus johannis
- plant extract
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References
Jain C, Khatana S, Vijayvergia R. Bioactivity of secondary metabolites of various plants: a review. Int J Pharm Sci Res. 2019;10(2):494-504. [DOI: 10.13040/IJPSR.0975-8232.10(2).494-04]
Satchanska G. Antibacterial activity of plant polyphenols: IntechOpen London, UK; 2022. [Link]
Gyawali R, Ibrahim SA. Natural products as antimicrobial agents. Food Control. 2014;46:412-29. [DOI:10.1016/j.foodcont.2014.05.047]
Efenberger-Szmechtyk M, Nowak A, Czyzowska A. Plant extracts rich in polyphenols: antibacterial agents and natural preservatives for meat and meat products. Crit Rev Food Sci Nutr. 2021;61(1):149-78. [DOI: 10.1080/10408398.2020.1722060] [PMID]
Beyzaei H, Ebrahimnezhad Z, Dehghani M. Antioxidant and Antimicrobial Effects of Nannorrhops Baluchestanica: An Endemic Palm Species in Southeast Iran. Jentashapir J Cell Mol Biol. 2023;14(1):e134760. [DOI:10.5812/jjcmb-134760]
Dehghani Kazemi M, Beyzaei H, Ebrahimnezhad Z. Chemical Composition, Antioxidant Potential, and Antimicrobial Activity of Nannorrhops ritchiana (Griff) Aitch. Archives of Advances in Biosciences. 2023;14(1):1-9. [DOI:10.22037/aab.v14i1.39282]
Clement WL, Bruun-Lund S, Cohen A, Kjellberg F, Weiblen GD, Rønsted N. Evolution and classification of figs (Ficus, Moraceae) and their close relatives (Castilleae) united by involucral bracts. Botanical Journal of the Linnean Society. 2020;193(3):316-39. [DOI:10.1093/botlinnean/boaa022]
Berg C, Corner E, Noteboom H. Moraceae (Ficus). Flora Malesiana, Series I (Seed plants,) Volume 17/Part 2. Leiden: National Herbarium of the Netherlands. 2005. [Link]
Frodin DG. History and concepts of big plant genera. Taxon. 2004;53(3):753-76. [DOI:10.2307/4135449]
Akhter R, Ghafoor A, College PA. Flora of Pakistan: Moraceae: Pakistan Agricultural College; 1985. [Link]
Khan kY, Khan M, Shah G, Hussain I. Ethnomedicinal uses of Genus Ficus L. in Pakistan. international poster journal of science and technology. 2011;1. [Link]
Haq N, Rashem W, Mubashir N. Phytochemical Composition, Antioxidant Potential, and Medicinal Significance of Ficus. In: Ibrahim K, Nesibe Ebru K, Ayzin K, Songül Ç, editors. Modern Fruit Industry. Rijeka: IntechOpen. 2019:8. [DOI: 10.5772/intechopen.86562]
Bordbar F, Mirtadzadini M. A new record of Ficus (Moraceae) for the flora of Iran. 2019;25:115-9. [DOI: 10.22092/IJB.2019.126196.1238]
Zolfaghari MR, Salimpour F, Sharifnia F, Jafari Marandi S. Analysis of genetic diversity among Iranian populations of Ficus carica L. (Moraceae). Acta Biologica Sibirica. 2019;5:131-8. [DOI: 10.14258/abs.v5.i3.6534]
Asghar A, Sharif A, Awan SJ, Akhtar B, Akhtar MF, Ali S, et al. "Ficus johannis Boiss. leaves ethanolic extract ameliorate streptozotocin-induced diabetes in rats by upregulating the expressions of GCK, GLUT4, and IGF and downregulating G6P". Environmental science and pollution research international. 2023;30(17):49108-24. [DOI: 10.1007/s11356-023-25765-4]
Afsharnezhad M, Shahangian SS, Sariri R. A novel milk-clotting cysteine protease from Ficus johannis: Purification and characterization. International Journal of Biological Macromolecules. 2019;121:173-82. [DOI:10.1016/j.ijbiomac.2018.10.006]
Tadavi SK, Dafare SW, Zanje SB, Rathod YU, Gadpayale JV. Preliminary phytochemical analysis of Ficus johanis Subsp.
afghanistanica from Moraceae family. J Pharmacogn Phytochem. 2023;12(1):41-3. [DOI: 10.22271/phyto.2023.v12.i1a.14546]
Thi Thu Ha P, Thi Bao Tran N, Thi Ngoc Tram N, Kha VH. Total phenolic, total flavonoid contents and antioxidant potential of Common Bean (Phaseolus vulgaris L.) in Vietnam. AIMS Agriculture and Food. 2020;5(4):635-48. [DOI: 10.3934/agrfood.2020.4.635]
Formagio AS, Volobuff CR, Santiago M, Cardoso CA, Vieira Mdo C, Valdevina Pereira Z. Evaluation of Antioxidant Activity, Total Flavonoids, Tannins and Phenolic Compounds in Psychotria Leaf Extracts. Antioxidants (Basel, Switzerland). 2014;3(4):745-57. [DOI: 10.3390/antiox3040745] [PMID] [PMCID]
Ebrahimnezhad Z, Dehghani M, Beyzaei H. Assessment of Phenolic and Flavonoid Contents, Antioxidant Properties, and Antimicrobial Activities of Stocksia Brahuica Benth. Int J Basic Sci Med. 2022;7(1):34-40. [DOI: 10.34172/ijbsm.2022.07]
Beyzaei H, Kamali Deljoo M, Aryan R, Ghasemi B, Zahedi MM, Moghaddam-Manesh MJCCJ. Green multicomponent synthesis, antimicrobial and antioxidant evaluation of novel 5-amino-isoxazole-4-carbonitriles. Chemistry Central Journal. 2018;12(1):1-8. [DOI:10.1186/s13065-018-0488-0]
Cockerill FR. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. (No Title). 2012. [Link]
Sun W, Shahrajabian MH. Therapeutic Potential of Phenolic Compounds in Medicinal Plants—Natural Health Products for Human Health. Molecules [Internet]. 2023; 28(4). [DOI:10.3390/molecules28041845]
Liu J, Zhao Y-T, Lu W-C, Huang P-H, Song T-Y, Li P-H. Bioactive Compounds in Malanto (Kalimeris indica) Leaves and Their Antioxidant Characteristics. Agriculture [Internet]. 2023; 13(1). [DOI:10.3390/agriculture13010211]
Mahmoudi S, Khali M, Benkhaled A, Benamirouche K, Baiti I. Phenolic and flavonoid contents, antioxidant and antimicrobial activities of leaf extracts from ten Algerian Ficus carica L. varieties. Asian Pacific Journal of Tropical Biomedicine. 2016;6(3):239-45. [DOI:10.1016/j.apjtb.2015.12.010]
Pękala-Safińska A, Tkachenko H, Kurhaluk N, Buyun L, Osadowski Z, Honcharenko V, et al. Studies on The Inhibitory Properties of Leaf Ethanolic Extracts Obtained from Ficus (Moraceae) Species Against Aeromonas Spp. Strains. Journal of veterinary research. 2021;65(1):59-66. [ DOI: 10.2478/jvetres-2021-0007]
H T, Buyun L, Osadowski Z, O K, V H, Prokopiv A. In vitro anti-Candida albicans efficacy of ethanolic extracts obtained from the leaves of some Ficus species (Moraceae). «Актуальні проблеми профілактичної медицини». 2017;1-2:65-76.[Link]
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