The Antimicrobial Effects of Ciprofloxacin Combined with Green Synthesized Glutathione-coated Silver Nanoparticles on Biofilm Formation of Pseudomonas Aeruginosa
Archives of Advances in Biosciences,
Vol. 9 No. 4 (2018),
23 December 2018
,
Page 24-34
https://doi.org/10.22037/jps.v9i4.22641
Abstract
Introdaction:Nowadays, antibiotic resistance is rising at an alarming rate. Essentially, one of the important ways for bacteria such as P. aeruginosa to survive in the presence of antibiotics is biofilm formation. In the current report, we have focused on inhibiting the microbial biofilm formation of P. aeruginosa through combining glutathione (GSH) coated silver nanoparticles (AgNPs) and Ciprofloxacin (Cip).Materials and Methods: AgNPs were biosynthesized using Eucalyptus Camaldulensis leaf extracts and surface modification of AgNPs was done, using glutathione. Synthesized nanoparticles were characterized by FTIR, XRD, DLS, SEM, and CHN tests. Then, 50 isolates of P. aeruginosa were originated from different samples of hospitalized patients from Sina hospital and 24 isolates were selected as a strong biofilm producer using microtiter plate method for further studies. Finally, the synergistic effect of GSH-coated AgNPs and Cip was investigated on biofilm formation of P. aeruginosa. Result:The images of SEM represent the spherical structure of silver nanoparticles with a smooth surface. Also, the results of FTIR, XRD, DLS, SEM, and CHN of AgNPs before and after surface coating confirmed the formation of GSH-coated AgNPs. GSH-coated AgNPs and Cip at a concentration of 1/2 and 1/4 MIC had an inhibitory activity on biofilm formation of 87.5% and 83.4% of P. aeruginosa isolates respectively. Conclusion:This study illustrated that the combination of GSH-coated AgNPs and Cip has a synergistic inhibitory activity on P. aeruginosa biofilm formation.
- Biofilm
- Eucalyptus camaldulensis
- Silver nanoparticles
- Synergistic
- Ciprofloxacin
How to Cite
References
Bergogne-Berezin E. Pseudomonas and miscellaneous Gram-negative bacilli. In: Cohen J, Powderly WG, editors. Infections Disease. 2nd ed. Philadelphia, PA: Mosby; 2004; 2203–2217.
Sharma, B.K., Saha, A., Rahaman, L., Bhattacharjee, S. and Tribedi, P. Silver Inhibits the Biofilm Formation of Pseudomonas aeruginosa. Advances in Microbiology 2015; 5, 677-685.
Wagner, V.E. and Iglewski, B.H. P. aeruginosa Biofilms in CF Infection. Clinical Reviews in Allergy & Immunology 2008; 35, 124-134.
Jadali F, Karimi A, Fallah F, Zahraei M, Esteghamati A,Navidinia M, et al. A survey on Rotavirus Associated Diarrhea in 5 Main Cities of Iran. Arch Pediatr Infect Dis.2013; 1(1): 23-6. DOI: 10.5812/pedinfect.6431
Eslami G, Fallah F, Taheri S, Navidinia M, Dabiri H, Dadashi M et al . Evaluation of antibacterial effect of cinnamon extract on Helicobacter pylori isolated from dyspeptic patients. Research in Medicine. 2013; 37 (2):85-89
Goudarzi M 1 , Seyedjavadi SS , Fazeli M , Roshani M , Azad M , Heidary M , Navidinia M , Goudarzi H .Identification of a Novel Cassette Array in Integronbearing Helicobacter Pylori Strains Isolated from Iranian Patients. Asian Pacific Journal of Cancer Prevention 2016, 17(7):3309-3315
Heydorn A, Ersboll B, Hentzer M, et al. Experimental reproducibility in flow-chamber biofilms. Microbiology 2000; 146: 2409–15.
Costerton JW, Cheng KJ, Geesey GG, Ladd TI, Nickel JC, Dasgupta M, Marrie TJ: Bacterial
biofilms in nature and disease. Ann Rev Microbiol 1987; 987:435–464.
Garrett T, Bhakoo M, Zhang Z. Bacterial adhesion and biofilms on surfaces. Progress in Natural Science 18 .2008; 1049–1056.
Emmerson, A. M., & Jones, A. M. The quinolones: decades of development and use. Journal of Antimicrobial Chemotherapy, 2003; 51(suppl 1), 13-20.
Herrlin, K., Segerdahl, M., Gustafsson, L. L., &Kalso, E. Methadone, ciprofloxacin, and adverse drug reactions. The Lancet, 2000; 356 (9247), 2069-2070.
Mederic M. Hall, MD, Jonathan T. Finnoff, DO, Jay Smith, MD. Musculoskeletal Complications of Fluoroquinolones: Guidelines and Precautions for Usage in the Athletic Population. PM&R J 2011; Vol. 3, Iss. 2. 132-142.
Zhang Y, Peng H, Huang W, Zhou Y, Yan D. Facile preparation and characterization of highly antimicrobial colloid Ag or Au nanoparticles. J Colloid Interface Sci 2008; 325:371‑6.
Das B, Dash SK, Mandal D, Adhikary J, Chattopadhyay S, Tripathy S, et al. Green-synthesized silver nanoparticles kill virulent multidrug-resistant Pseudomonas aeruginosa strains: A mechanistic study. BLDE Univ J Health Sci 2016; 1: 89-101.
N. Duran, P.D. Marcato, R. De Conti, O.L. Alves, F.T.M. Costa, M. Brocchi, J. Brazil. Potential use of silver nanoparticles on pathogenic bacteria, their toxicity and possible mechanisms of action. Chem. Soc. 21 .2010; 949–959.
Mudshinge SR, Deore AB, Patil S, Bhalgat CM: Nanoparticles: emerging carriers for drug delivery. Saudi Pharmaceutical Journal 2011; 19(3):129–141.
A.F.Abd El-Rahman, Tahany.G.M. Mohammad, Green synthesis of silver nanoparticle using Eucalyptus globulus leaf extract and its antibacterial activity. Journal of Applied Sciences Research 2013; 9(10), 6437-6440.
Mittal AK, Chisti Y, Banerjee UC. Synthesis of metallic nanoparticles using plant extracts. Biotechnol 2013; Adv 31: 346-356.
Djenane D, Yangüela J, Amrouche T, Boubrit S, Boussad N, Roncalés P. Chemical composition and antimicrobial effects of essential oils of Eucalyptus globulus, Myrtus communis and Satureja hortensis against Escherichia coli O157:H7 and Staphylococcus aureus in minced beef. Food Sci Technol Int 2011; 17: 505-15.
Javaid A, Samad S. Screening of allelopathic trees for their antifungal potential against Alternaria alternata strains isolated from dying-back Eucalyptus spp. Nat Prod Res 2012; 26: 1697-702.
I.S. Lim, D. Mott, W. Ip, P.N. Njoki, Y. Pan, S.Q. Zhou, C.J. Zhong, Interparticle interactions in glutathione mediated assembly of gold nanoparticles, Langmuir 24, 2008; 8857–8863.
I.V. Anambiga, V. Suganthan, N. Arunai Nambi Raj,G. Buvaneswari, T. S. Sampath Kumar. Colorimetric Detection Of Lead Ions Using Glutathione Stabilized Silver Nanoparticles. IJSER 2013; Vol 4, Iss 5, 710-715.
Z. Aghajani Kalaki, R. Safaeijavan, M. Mahdavi Ortakand. Biosynthesis of Silver Nanoparticles Using Mentha longifolia (L.) Hudson Leaf Extract and Study its Antibacterial Activity. JPS 2017; 8 (2), 24-30.
Z.A. Kalaki, R. SafaeiJavan, H. Faraji. Procedure optimisation for green synthesis of silver nanoparticles by Taguchi method.IET Micro & Nano Letters. 2018; 13 (4), 558-561.
Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun1982; 37:318-26.
Li P, Li J, Wu C, Wu Q, Li J. Synergistic antibacterial effects of β-lactam antibiotic combined with silver nanoparticles. Nanotechnology. Jul 28 2005; 16(9) 12-19.
Bashir T, Zahid Qureshi M. Photosynthesis of Silver nanoparticles using E. Camaldulensis Leaf Extract and Their characterization. J. Chil. Chem 2015; Soc. vol.60. 2861-63.
Velhal et al. Taguchi Design for Parameter Optimization of Size-Controlled Synthesis of Silver Nanoparticles. International Journal of Emerging Technologies in Computational and Applied Sciences 2015; 12(2): 144-149.
Ramezani Ali Akbari K, Abdi Ali A. Study of antimicrobial effects of several antibiotics and iron oxide nanoparticles on biofilm producing pseudomonas aeruginosa. Nanomed J. 2017; 4(1): 37-43.
Taglietti A, Diaz Fernandez YA, Amato E, Cucca L, Dacarro G, Grisoli P, Necchi V, Pallavicini P, Pasotti L, Patrini M. Antibacterial activity of glutathione-coated silver nanoparticles against gram positive and gram negative bacteria. Langmuir. 2012 May 15; 28 (21):8140-8.
- Abstract Viewed: 330 times
- PDF Downloaded: 227 times