Regeneration, Reconstruction & Restoration (Triple R)

Vol. 10 (2025)

In-Vitro Comparison of the Antibacterial Activity of Probiotic Yogurt, Cheese, and Dough Against Two Common Oral Micro-Organisms Antibacterial Activity of Probiotic Products

Regeneration, Reconstruction & Restoration (Triple R), Vol. 10 (2025), 1 January 2025
https://doi.org/10.22037/rrr.v10.48297

Abstract

Background and objectives: There are several types of micro-organisms existing in the oral cavity that can lead to tooth decay. Probiotics are effective in reducing the level of these species. The aim of this study is a laboratory comparison of the antibacterial activity of three types of probiotic products on two common oral micro-organisms.

Materials and methods: In this experimental study, the minimum inhibitory concentration (MIC), as well as the bacterial inhibition zone diameter of probiotic dough, yogurt, and cheese containing Lactobacillus acidophilus and Bifidobacterium, against the micro-organisms were measured Following normality testing, group comparisons were performed using Kruskal-Wallis and Mann-Whitney U tests. Statistical significance was defined as P < 0.05.

Results: The results suggested a significant difference in the MIC of Enterococcus faecalis and Streptococcus mutans. The MIC of probiotic yogurt and cheese was significantly higher than that of the non-probiotic ones. Moreover, the level of MIC in the non-probiotic dough was significantly higher than that in the probiotic version.

Conclusion: The results of this in-vitro study suggest that probiotic dough, unlike yogurt and cheese, was more effective than non-probiotic types in reducing oral Enterococcus faecalis and Streptococcus mutans.

Keywords:
  • Probiotics
  • Streptococcus mutans
  • Enterococcus Faecalis
  • Minimum inhibitory concentration

How to Cite

1.
Rayani A, Ahanjan M, Hosseini M, Mollaei M, Zamanzadeh M. In-Vitro Comparison of the Antibacterial Activity of Probiotic Yogurt, Cheese, and Dough Against Two Common Oral Micro-Organisms: Antibacterial Activity of Probiotic Products . Regen Reconstr Restor [Internet]. 2025 Oct. 19 [cited 2026 Aug. 22];10. Available from: https://journals.sbmu.ac.ir/tripleR/article/view/48297

References

1. Yu OY, Zhaols MG, Mei ML, Lo Ec, Chu CH. Dental biofilm and labarotory microbial culture models for cariology research. Dent J 2017; 5 (2): e21.

2. Sanpinit S, Moosigapong K, Jarukitsakul S, Jatutasri K, Issuriya A, Joycharat N, Maneenoon K, Jaisamut P, Chusri S, Voravuthikunchai SP, Jetwanna KW. Selected Thai traditional polyherbal medicines suppress the cariogenic properties of Streptococcus mutans by disrupting its acid formation and quorum sensing abilities. South African Journal of Botany. 2022 Jan 1;144:355-63.

3. Aqawi M, Sionov RV, Gallily R, Friedman M, Steinberg D. Anti-bacterial properties of cannabigerol toward Streptococcus mutans. Frontiers in Microbiology. 2021 Apr 22;12:922.

4. Sadri L, Taghian M, Rahimian A, Hosseinnataj A, Goli H. Antibacterial Effect of Bitter and Sweet Almond Hydroalcoholic Extracts on Streptococcus mutans, Lactobacillus acidophilus, Enterococcus faecalis, Streptococcus salivarius, and Streptococcus sobrinus: Antibacterial Effect of Bitter and Sweet Almond. Regeneration, Reconstruction & Restoration (Triple R).;9.

5. OmerOglou E, Karaca B, Kibar H, Haliscelik O, Kiran F. The role of microbiota-derived postbiotic mediators on biofilm formation and quorum sensing-mediated virulence of Streptococcus mutans: A perspective on preventing dental caries. Microbial Pathogenesis. 2022 Jan 26:105390.

6. Murray jj, Nunn jh, G.Steel j, Prevetiom of oral Disease, 4nd ed New York: ox ford university press,2003.P:96.

7. Jabbareh L, Ehsani H, Goli H, Hosseinnataj A, Ebadian S, Mollaei M, Etezadi T. Comparing the inhibitory effect of kefir drink and chlorhexidine mouthwash against oral bacteria in orthodontic patients. Journal of Dentistry. 2025 Jun 1;26(2):171.

8. World health organization. Guidelines for thr evaluation of probiotics in organization. Guidelines for the evaluation of probiotics in food [cited2002]

9. Mardani M, Rezapour S. The Effect of Probiotics Supplementation on Maternal-Fetal Care. Current Women's Health Reviews. 2021 Feb 1;17(1):29-35.

10. Sizentsov A, Salnikova E, Sizentsov Y, Peshkov S, Barysheva E, Naumenko O, Romanenko N. Technology for evaluating the ability of probiotic strains to accumulate copper ions in vitro and in vivo using Bacillus bacteria. Veterinary World. 2021 Jul;14(7):1752.

11. Biria M, Eslami G Taghipour E Baghban AA. Effects of three mastic gums on number of mutans streptococci, lactobacilli and pH of the saliva, Journal of dentistry (Tehran,Iran).2014-11(6):672.

12. Nishinar T, Suzuki N,yonedo M, Hirofujit: Effects of Lactobacillus salivarius-containing tablets on caries risk factor: A randomized open-label clinical trial. BMC oral Health: 2014;14:110.

13. Caglar E,Kuscu OO,Selvi Kuvvelti S,Kavaloglu Cildir S,Sandalli N.short-term effect of ice cream containing

bifidobacterium Lactis Bb-12 on the number of salivary mutans streptococci and lactobacilli Acta Odontol Scand2008:66(3):154-8.

14. Amargianitakis M, Antoniadou M, Rahiotis C, Varzakas T. Probiotics, Prebiotics, Synbiotics and Dental Caries. New Perspectives, Suggestions, and Patient Coaching Approach for a Cavity-Free Mouth. Applied Sciences. 2021 Jan;11(12):5472.

15. Cogulu d, topaliglu –ak a, cagalr e sandalli n, karagozlu c ersin n etal potential effects od a miltistrain probotic-kefir on salivary Streptococcus mutans and Lactobacillius Spp.j dent sci 2010:5(3):144-149.

16. Jabbareh L, Ehsani H, Goli H, Nattaj AH, Ebadian S, Mollaei M, Etezadi T. Comparing the Inhibitory Effect of Kefir Drink and Chlorhexidine Mouthwash against Oral Bacteria in Orthodontic Patients. Journal of Dentistry. 2024 Nov 12.

17. Simark MC, Emilson CG, Grahn HE, Jacobsson C, Roos K, Holm S. Lactobacillus mediated interference of mutans streptococci in caries free vs caries active subjects. Eur J Oral Sci 2007; 115:308314.13.

18. Soderling EM, Marttinen AM, Haukoija AL. Probiotic lactobacilli interfere with Streptococcus mutans biofilm formation in vitro. Curr Microbiol2011;62(2):618-22.

19. Farnworth ER. Kefir a complex probiotic. Food Sci Technol Bull Functional Foods 2005;2(1):1-17.

20. Rayani A, Ahanjan M, Goli HR. Comparing the Effect of Probiotic and Non-probiotic Yogurt Drinks on Two Common Oral Microorganisms: An In Vitro Study. Journal of Mazandaran University of Medical Sciences. 2020 May 10;30(185):33-40.

21. Lesan S, Rahbar M, Valaei N, Abbasi Vardoogh S, Abbasi Vardoogh M. Effect of probiotic yoghurt on salivary Streptococcus mutans and Lactobacillus. Research in Medicine. 2013 Nov 1;37(3):151-7.

22. Ahola AJ, Yli-Knuuttila H, Suomalainen T, Poussa T, Ahlström A, Meurman JH, Korpela R. Short-term consumption of probiotic-containing cheese and its effect on dental caries risk factors. Archives of oral biology. 2002 Nov 1;47(11):799-804.

23. Hatakka K, Ahola AJ, Yli-Knuuttila H, Richardson M, Poussa T, Meurman JH, Korpela R. Probiotics reduce the prevalence of oral Candida in the elderly—a randomized controlled trial. Journal of dental research. 2007 Feb;86(2):125-30.

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