The Evaluation of Antioxidant Activity and Cytotoxicity of Leaf, Orange Fruit, and Calyx Extract of Physalis alkekengi on Human Lung Cancer A549 Cell Line
Regeneration, Reconstruction & Restoration (Triple R),
Vol. 5 (2020),
24 March 2020
,
Page e26
https://doi.org/10.22037/rrr.v5i.32950
Abstract
Introduction: Lung cancer is the most common cancers, and chemotherapy treatment as one of the methods leads to the occurrence of intolerable side effect. Thus recently, natural products extracted from medicinal plants can play an important role in cancer treatment. It was aimed in this study, based on medicinal effect of the Physalis alkekengi, to determine the content and activity of some antioxidant compounds of leaves, orang fruits, and calyx of P. alkekengi and also the inhibitory effects of their crude extract on lung cancer A549 cell line.
Materials and Methods: Leaf, Orange Fruit, and Calyx Extract of P. alkekengi were harvested from the region of Tonekabon city and A549 cells line was cultivated and proliferated. Then, the cells exposed to different concentrations of the leaves, fruits ,and calyx extract of P. alkekengi (31.25 to 2000 μg/ml) were incubed for 24, 48, and 72 hours. After the incubation period, the colorimetric MTT method was used to determine cytotoxicity. Also, total phenol, flavonoid, anthocyanin, and carotenoid contents were determined as well as antioxidant activity of extract using DPPH method.
Results: The results showed that the highest total phenol, flavonoids, and anthocyanins were in leaves and the highest antioxidant activity and total carotenoids were seen in fruits. The results have also shown that the extracts of leaves, fruits, and calyxes brought the highest inhibition of cancer cell growth at the concentration level of 2000 μg/ml that were 88.55, 91.81, and 94.97% respectably.
Conclusion: These results suggest that the leave, fruit and calyx of ethanolic extract of P. alkekengi have the most antioxidant and antimicrobial activity and cytotoxicity against A549 cell line. It seems to come with further research, and utilizes its compound in cancer treatment.
- Antioxidant Compounds
- Lung Cancer
- A549 cells line
- Physalis Alkekengi
How to Cite
References
2. Cersosimo RJ. Lung cancer. American Journal of Health-System Pharmacy. 2000; 59 (7): 611-42.
3. Chang C, Yang M, Wen H, Chern J. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 2002; 10: 178-182.
4. Fauci AS, Braunwald E, Kasper DL, Hauser SL, Longo DL. Har-rison’s Principles of internalmedicine. 17th ed. Philadelphia: Mc Graw Hill, 2008, 551-562.
5. Fridlender M, Kapulnik Y, Koltai H. Plant derived substances with anti-cancer activity: From folklore to practice. Frontiers in Plant Science. 2015; 6:1-9.
6. Greenwell M, Rahman PK. Medicinal Plants: Their Use in Anticancer Treatment. International Journal of Pharmaceutical Science Research. 2015; 6: 4103-4112.
7. Hajian KFA, Kia MT. Pattern of age distribution of different cancers in Babol in 2001. Pajouhesh dar Pezeshki. 2003; 27 (3): 239-245.[ Persian in text full].
8. Horn L, Pao W, Johnson DH, Loscalzo J. Harrison's Principles of Internal Medicine. 2012; (89): 18-100.
9. Iqbal J, Abbasi BA, Mahmood T, Kanwal, S, Ali B, Shah SA, Khali AT. Plant-derived anticancer agents: A green anticancer approach. Asian Pacific Journal Trop Biomed. 2017; 7(12): 1129-1150.
10. Ji L, Yuan Y, Luo L, Chen Z, Ma X, Ma Z. Physalins with anti-inflammatory activity is present in Physalis alkekengi var. franchetii and can function as Michael reaction acceptors. Steroids. 2012; 77(5): 441-447.
11. Kandaswami C, Lee LT, Lee PP, Hwang JJ, Ke FC, Huang YT, Lee MT. The antitumor activities of flavonoids. In vivo. 2005; 19: 895-909.
12. Krishnamurthi K. 17-screening of natural products for anticancer and antidiabetic properties. Cancer 2007; 3: 4.
13. Lichtenthaler, H.K. Chlorophylls and Carotenoids; Pigments of Photosynthetic Membranes. Methods Enzaymol. 1987; 148: 350-382.
14. Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG. Determination of the Total Phenolic, Flavonoid and Pralin Contents in Burkina Fasan Honey, as well as Their Scavenging Activity. Food Chemistry. 2005; 91: 571-577.
15. Miliauskas G, Venskutonis PR, Vanbeek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry. 2004; 85: 231-237.
16. Mita S, Murano N, Akaike M, Nakamura K. Mutants of Arabidopsis thaliana with Pleiotropic Effects on the Expression of the Gene for Beta-amylase and on the Accumulation of Anthocyanin Those is Inducible by Sugars. Plant Journal. 1997; 11: 841-851.
17. Orfali GC, Duarte AC, Bonadio V. Review of anticancer mechanisms of isoquercitin. World Journal of Clinical Oncology. 2016; 7 (2): 189-199.
18. Parkin DM, Bray F, Ferlay J, Pisani P. Global cancer statistics, 2002. Cancer Journal for Clinicals. 2005; 55(2): 74-108.
19. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnology. 2006; 5: 1142-1145.
20. Ren W. Flavonoids: promising anticancer agents. Medicinal Research Reviews. 2003; 23: 519-34.
21. Silvestri GA, Alberg AJ, Ravenel J. The changing epidemiology of lung cancer with a focus on screening. British Medical Journal. 2009; 339: 451-54.
22. Sirvastava V, Negi AS, Kumar JK, Gupta MM, Khanuja SP. Plant-based anticancer molecules: a chemical and biological profile of some important leads. Bioorganic and Medicinal chemistry. 2005; 13: 5892-5908.
23. Sitiasma MJ, Al-jamal H, Yong-Ang CH, Matasan J, Seeni A, Johan MF. Apoptosis induction in MV4-11 and K562 human leukemic cells by Pereskia sacharosa (Cactaceae) leaf crude extract. Asian Pacific Journal of Cancer Prevention. 2014; 15 (1): 475-481.
24. Solowey E, Lichtenstein M, Sallon S, Paavilainen H. Evaluating Medicinal Plants for Anticancer Activity. The Scientific World Journal. 2014; 12: 1-12.
25. Tariq A, Sadia S, Pan K, Ullah I, Mussarat S, Sun F. A systematic review on ethnomedicines of anti-cancer plants. Phototherapy Research. 2017; 31: 202-64.
26. Thakore P, Mani RK, Kavitha SJ. A brief review of plants having anti-cancer property. International Journal of Pharmaceutical Research Development. 2012; 3: 129-36.
27. Velasco G, Hernández-Tiedra S, Dávila D, Lorente M. The use of cannabinoids as anticancer agents. Progress in Neuro-Psychopharmacology and Biological Psychiatry. 2016; 64: 259-266.
28. Wang Y, Wang J, Wang H, Ye W. Novel taxane derivatives from Taxus wallichiana with high anticancer potency on tumor cells. Chemical Biology and Drug Design. 2016; 88(4): 556-561.
29. Yoshida M, Sakai T, Hosokawa N, Marui N, Matsumoto K, Fujioka A, Nishino H, Aoike A. The effect of quercetin on cell cycle progression and growth of human gastric cancer cells. FEBS Letters. 1990; 260:10-13.
30. Zargari A. Medical plants. Tehran University, Tehran, 2003; 3595-3596.
- Abstract Viewed: 221 times
- PDF Downloaded: 134 times