The Effect of Vitamin K2 on Osteogenic Differentiation of Dental Pulp Stem Cells: An In Vitro Study
Regeneration, Reconstruction & Restoration (Triple R),
Vol. 2 No. 1 (2017),
1 May 2017
,
Page 26-29
https://doi.org/10.22037/rrr.v2i1.18536
Abstract
Introduction: dental pulp stem cells (DPSCs) have been shown to have great capacity to differentiation toward the osteoblast lineage and they can be considered as a great cell source for bone tissue engineering. The vitamin K family, especially vitamin K2 (MK-4), have been shown to have an osteoprotective role. In this study, we have investigated the effect of various concentrations of MK-4 on differentiation of DPSCs into osteoblast.
Materials and Methods: DPSCs were isolated and characterized to expression the mesenchymal markers. These cells were treated with osteogenic medium with and without of various concentrations of MK-4 for 14 days. Osteogenic capability and extracellular calcium deposition were assessed by ALP assay and alizarin red staining, respectively, at zero, 7, 14 days after induction.
Result: the additional of MK-4 at concentration of 10 µM with osteogenic medium had a significant effect on differentiation DPSCs into osteoblast (P<0.05) at 14 day, as it confirmed by both ALP activity assay and alizarin red staining.
Conclusion: MK-4 can promote differentiation of DPSCs into osteoblast in vitro so have a potential to be considered in improvement of cell-based bone tissue engineering therapies.
- Mesenchymal stem cells
- Dental pulp stem cells
- Vitamin K
- Osteogenesis
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