Exploring the Reversible Effects of UV Laser Radiation on the Gene Expression Profiles of Saccharomyces cerevisiae Via Network Analysis Reversible Effects of UV Laser Radiation on Saccharomyces cerevisiae
Journal of Lasers in Medical Sciences,
Vol. 12 (2021),
13 Bahman 2021
,
Page e90
Abstract
Introduction: The reversibility of biological processes is an important challenge in the study of environmental pollutants and also natural and artificial radiation. There are many pieces of evidence about the reversible and irreversible effects of UV radiation on the human body. Assessment of the reversibility of UV laser effects on Saccharomyces cerevisiae was the aim of this study.
Methods: Gene expression alteration in S. cerevisiae samples radiated by a 30s UV laser for 15, 30, and 60 minutes post-radiation times were investigated via network analysis to explore time-dependent reversible alteration in the gene expression profiles of the samples.
Results: 19 differentially expressed genes (DEGs) were identified as targeted genes for the samples which were harvested 60 minutes after radiation; network analysis revealed no significant alteration in biological processes.
Conclusion: It can be concluded that the gross effects of the UV laser on S. cerevisiae samples disappear after 60 minutes of radiation.
Keywords:
- Post-radiation time, Network analysis, Saccharomyces cerevisiae, Repair, Radiation
How to Cite
rezaei-tavirani, mostafa, Vafaee, R. ., Safari-Alighiarloo, N. ., Razzaghi, Z. ., Ansari, M., Rostami-Nejad, M. ., Hamdieh, M., & Mansouri, V. . (2021). Exploring the Reversible Effects of UV Laser Radiation on the Gene Expression Profiles of Saccharomyces cerevisiae Via Network Analysis: Reversible Effects of UV Laser Radiation on Saccharomyces cerevisiae. Journal of Lasers in Medical Sciences, 12, e90. Retrieved from https://journals.sbmu.ac.ir/jlms/article/view/36866
References
1. Jan H. HJ Genome maintence mechanisms for preventig cancer. Nature. 2001;411:366-74.
2. Basu AK. DNA damage, mutagenesis and cancer. International journal of molecular sciences. 2018;19(4):970.
3. Friedberg E, Walker G, Siede W, Wood R, Schultz R, Ellenberger T. DNA repair and mutagenesis. ASM Press. Washington, DC. 2006;957.
4. de Laat A, van Tilburg M, van der Leun JC, van Vloten WA, de Gruijl FR. Cell cycle kinetics following UVA irradiation in comparison to UVB and UVC irradiation. Photochemistry and photobiology. 1996;63(4):492-7.
5. Modenese A, Korpinen L, Gobba F. Solar radiation exposure and outdoor work: an underestimated occupational risk. International journal of environmental research and public health. 2018;15(10):2063.
6. Gasch AP, Spellman PT, Kao CM, Carmel-Harel O, Eisen MB, Storz G, et al. Genomic expression programs in the response of yeast cells to environmental changes. Molecular biology of the cell. 2000;11(12):4241-57.
7. Hauser M, Abraham PE, Barcelona L, Becker JM. UV laser-induced, time-resolved transcriptome responses of Saccharomyces cerevisiae. G3: Genes, Genomes, Genetics. 2019;9(8):2549-60.
8. Prakash S, Prakash L. Nucleotide excision repair in yeast. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2000;451(1-2):13-24.
9. Gratchev A, Strein P, Utikal J, Goerdt S. Molecular genetics of Xeroderma pigmentosum variant. Experimental dermatology. 2003;12(5):529-36.
10. Yin Y, Petes TD. Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae. PLoS genetics. 2013;9(10):e1003894.
11. Abbaszadeh H-A, Peyvandi AA, Sadeghi Y, Safaei A, Zamanian-Azodi M, Khoramgah MS, et al. Er: YAG laser and cyclosporin A effect on cell cycle regulation of human gingival fibroblast cells. Journal of lasers in medical sciences. 2017;8(3):143.
12. Safari-Alighiarloo N, Taghizadeh M, Tabatabaei SM, Namaki S, Rezaei-Tavirani M. Identification of common key genes and pathways between type 1 diabetes and multiple sclerosis using transcriptome and interactome analysis. Endocrine. 2020;68(1):81-92.
13. Lu L, Yang N, Cai Y. Well-controlled reversible addition–fragmentation chain transfer radical polymerisation under ultraviolet radiation at ambient temperature. Chemical communications. 2005(42):5287-8.
14. Sinha R, Häder D-P. Life under solar UV radiation in aquatic organisms. Advances in Space Research. 2002;30(6):1547-56.
15. Yan Y, Stoddard FL, Neugart S, Sadras VO, Lindfors A, Morales LO, et al. Responses of flavonoid profile and associated gene expression to solar blue and UV radiation in two accessions of Vicia faba L. from contrasting UV environments. Photochemical & Photobiological Sciences. 2019;18(2):434-47.
16. Rostami-Nejad M, Razzaghi Z, Esmaeili S, Rezaei-Tavirani S, Baghban AA, Vafaee R. Immunological reactions by T cell and regulation of crucial genes in treated celiac disease patients. Gastroenterology and hepatology from bed to bench. 2020;13(2):155.
17. Salari R, Salari R. Investigation of the best Saccharomyces cerevisiae growth condition. Electronic physician. 2017;9(1):3592.
18. Bisquert R, Muñiz-Calvo S, Guillamón JM. Protective role of intracellular melatonin against oxidative stress and UV radiation in Saccharomyces cerevisiae. Frontiers in Microbiology. 2018;9:318.
19. Li W, Adebali O, Yang Y, Selby CP, Sancar A. Single-nucleotide resolution dynamic repair maps of UV damage in Saccharomyces cerevisiae genome. Proceedings of the National Academy of Sciences. 2018;115(15):E3408-E15.
20. Guo X, Zhang M, Liu R, Gao Y, Yang Y, Li W, et al. Repair characteristics and time-dependent effects in Saccharomyces cerevisiae cells after X-ray irradiation. World Journal of Microbiology and Biotechnology. 2019;35(1):1-15.
21. Guintini L, Tremblay M, Toussaint M, D’Amours A, Wellinger RE, Wellinger RJ, et al. Repair of UV-induced DNA lesions in natural Saccharomyces cerevisiae telomeres is moderated by Sir2 and Sir3, and inhibited by yKu–Sir4 interaction. Nucleic acids research. 2017;45(8):4577-89.
2. Basu AK. DNA damage, mutagenesis and cancer. International journal of molecular sciences. 2018;19(4):970.
3. Friedberg E, Walker G, Siede W, Wood R, Schultz R, Ellenberger T. DNA repair and mutagenesis. ASM Press. Washington, DC. 2006;957.
4. de Laat A, van Tilburg M, van der Leun JC, van Vloten WA, de Gruijl FR. Cell cycle kinetics following UVA irradiation in comparison to UVB and UVC irradiation. Photochemistry and photobiology. 1996;63(4):492-7.
5. Modenese A, Korpinen L, Gobba F. Solar radiation exposure and outdoor work: an underestimated occupational risk. International journal of environmental research and public health. 2018;15(10):2063.
6. Gasch AP, Spellman PT, Kao CM, Carmel-Harel O, Eisen MB, Storz G, et al. Genomic expression programs in the response of yeast cells to environmental changes. Molecular biology of the cell. 2000;11(12):4241-57.
7. Hauser M, Abraham PE, Barcelona L, Becker JM. UV laser-induced, time-resolved transcriptome responses of Saccharomyces cerevisiae. G3: Genes, Genomes, Genetics. 2019;9(8):2549-60.
8. Prakash S, Prakash L. Nucleotide excision repair in yeast. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2000;451(1-2):13-24.
9. Gratchev A, Strein P, Utikal J, Goerdt S. Molecular genetics of Xeroderma pigmentosum variant. Experimental dermatology. 2003;12(5):529-36.
10. Yin Y, Petes TD. Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae. PLoS genetics. 2013;9(10):e1003894.
11. Abbaszadeh H-A, Peyvandi AA, Sadeghi Y, Safaei A, Zamanian-Azodi M, Khoramgah MS, et al. Er: YAG laser and cyclosporin A effect on cell cycle regulation of human gingival fibroblast cells. Journal of lasers in medical sciences. 2017;8(3):143.
12. Safari-Alighiarloo N, Taghizadeh M, Tabatabaei SM, Namaki S, Rezaei-Tavirani M. Identification of common key genes and pathways between type 1 diabetes and multiple sclerosis using transcriptome and interactome analysis. Endocrine. 2020;68(1):81-92.
13. Lu L, Yang N, Cai Y. Well-controlled reversible addition–fragmentation chain transfer radical polymerisation under ultraviolet radiation at ambient temperature. Chemical communications. 2005(42):5287-8.
14. Sinha R, Häder D-P. Life under solar UV radiation in aquatic organisms. Advances in Space Research. 2002;30(6):1547-56.
15. Yan Y, Stoddard FL, Neugart S, Sadras VO, Lindfors A, Morales LO, et al. Responses of flavonoid profile and associated gene expression to solar blue and UV radiation in two accessions of Vicia faba L. from contrasting UV environments. Photochemical & Photobiological Sciences. 2019;18(2):434-47.
16. Rostami-Nejad M, Razzaghi Z, Esmaeili S, Rezaei-Tavirani S, Baghban AA, Vafaee R. Immunological reactions by T cell and regulation of crucial genes in treated celiac disease patients. Gastroenterology and hepatology from bed to bench. 2020;13(2):155.
17. Salari R, Salari R. Investigation of the best Saccharomyces cerevisiae growth condition. Electronic physician. 2017;9(1):3592.
18. Bisquert R, Muñiz-Calvo S, Guillamón JM. Protective role of intracellular melatonin against oxidative stress and UV radiation in Saccharomyces cerevisiae. Frontiers in Microbiology. 2018;9:318.
19. Li W, Adebali O, Yang Y, Selby CP, Sancar A. Single-nucleotide resolution dynamic repair maps of UV damage in Saccharomyces cerevisiae genome. Proceedings of the National Academy of Sciences. 2018;115(15):E3408-E15.
20. Guo X, Zhang M, Liu R, Gao Y, Yang Y, Li W, et al. Repair characteristics and time-dependent effects in Saccharomyces cerevisiae cells after X-ray irradiation. World Journal of Microbiology and Biotechnology. 2019;35(1):1-15.
21. Guintini L, Tremblay M, Toussaint M, D’Amours A, Wellinger RE, Wellinger RJ, et al. Repair of UV-induced DNA lesions in natural Saccharomyces cerevisiae telomeres is moderated by Sir2 and Sir3, and inhibited by yKu–Sir4 interaction. Nucleic acids research. 2017;45(8):4577-89.
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