Light-Emitting Diode Laser Therapy for HyperoxiaInduced Retinal Abnormalities LED Laser Therapy for Retinal Hyperoxia
Journal of Lasers in Medical Sciences,
Vol. 12 (2021),
13 February 2021
,
Page e64
Abstract
Introduction: Hyperoxygenation is linked to numerous effects in a variety of organ systems. It can cause tissue damage by generating reactive oxygen species (ROS), increasing oxidative stress, and inducing cell death by apoptosis. The present study aimed to evaluate the effects of low-level laser therapy on the retina in response to acute hyperoxia in animals.
Methods: A total of 70 Wistar albino rats were evaluated in the present study: 10 rats were designated as a control group, and the rest were exposed to hyperoxia (O2, 90%) for 3 days, 1 week, and 2 weeks (20 rats each). Each group was divided into two subgroups (n=10), one of which was designated as hyperoxia only. The other was treated with a 670 nm light-emitting diode laser (2 sessions/one week, ~ 9.0 J/cm2) in each eye. The animals were euthanized, and their retinas were dissected for analysis of protein content, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), total antioxidant capacity (TAC), hydrogen peroxide (H2O2), malondialdehyde (MDA), and histological examination.
Results: We found that two weeks of hyperoxia-induced an increase in retinal protein content (P<0.001), an alteration in the intensities and molecular weights of protein fractions, a significant decrease in the TAC level (P<0.01), and a noticeable increase in H2 O2 and MDA levels (P<0.001). Histological examination revealed fragmentation of the photoreceptors and neovascularization in the outer and inner plexiform layers. Furthermore, the data showed remarkable improvement in the retinal protein contents, oxidative state, and retinal structure after light-emitting diode laser therapy.
Conclusion: Light-emitting diode laser therapy was found to be a useful treatment paradigm for reducing hyperoxia-induced retinal damage.
Keywords:
- Hyperoxia- Light emitted diode laser therapy- Retinal protein- Oxidative stress- Histological examination
How to Cite
Abd Eldaiem, M. S. ., Abdelkawi, S. A., Elsaeid, A. A., Hassan, A. A., Ghoneim, D. fouad ., & El Rashedi, A. . I. . (2021). Light-Emitting Diode Laser Therapy for HyperoxiaInduced Retinal Abnormalities: LED Laser Therapy for Retinal Hyperoxia. Journal of Lasers in Medical Sciences, 12, e64. Retrieved from https://journals.sbmu.ac.ir/jlms/article/view/34098
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26. Terraneo L, Virgili E, Caretti A, Bianciardi P, Smaja M. In vivo hyperoxia induces hypoxia- inducible factor-1α overexpression in LNCaP tumor without affecting the tumor growth rate. Int J Biochem Cell biol. 2004; 51: 65-74.
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28. Penn JS, Henry MM, Tolman BL. Exposure to Alternating Hypoxia and Hyperoxia Causes Severe Proliferative Retinopathy in the Newborn Rat. Pediatr Res. 1994;36(6):724–31.
29. Yanik M, Erel O, Kati M. The relationship between potency of oxidative stress and severity of depression. Acta Neuropsychiatr. 2004; 16: 200-203.
30. Harma M, Harma M, Erel O. Oxidative stress in women with preeclampsia. Am J Obstet Gynecol. 2005; 192: 656–657.
31. Yeni E, Gulum M, Selek S, Erel O, Unal D, Verit A, et al. Comparison of oxidative/antioxidative status of penile corpus cavernosum blood and peripheral venous blood. Int J Impot Res. 2005; 17: 19-22.
32. Graziosi A, Perrotta M , Russo D, Gasparroni G, D’Egidio C, Marinelli B , et al. Oxidative stress markers and the retinopathy of prematurity. J Clin Med. 2020; 9(9): 2711.
33. Liu W, Chen Q, Liu J , Liu KJ. Normobaric hyperoxia protects the blood brain barrier through inhibiting Nox2 containing NADPH oxidase in ischemic stroke. Medical Gas Res. 2011; 1(22):1-8.
34. Demirbag R, Gur M, Yilmaz R, Kunt AS, Erel O, Andac MH. Influence of oxidative stress on the development of collateral circulation in total coronary occlusions. Int J Cardiol. 2007; 116: 14-19.
35. Rabus M, Demirbağ R, Sezen Y, Konukoğlu O, Yildiz A, Erel Ö, et al. Plasma and tissue oxidative stress index in patients with rheumatic and degenerative heart valve disease. Arch Turk Soc Cardiol. 2008; 36: 536-540.
36. Shan Y, Ye X, Xin H. Effect of the grape seed proanthocyanicin extract on the free radical and energy metabolism indicators during the movement. Sci Res Essay. 2010; 5:148-153.
37. Bowers, F, Valter K, Chan S, Walsh N, Maslim J, Stone J. Effects of oxygen and bFGF on the vulnerability of photoreceptors to light damage. Invest Ophthalmol Vis Sci. 2001; 42(3): 804-815.
38. Dorfman A, Dembinska O, Chemtob S, Lachapelle P. Early manifestations of postnatal hyperoxia on the retinal structure and function of the neonatal rat. Invest Ophthalmol Vis Sci. 2008; 49(1): 458-466.
39. Heinig N, Schumann U, Calzia, D, Panfoli I, Ader M, Schmidt M H, et al. Photobiomodulation Mediates Neuroprotection against Blue Light Induced Retinal Photoreceptor degeneration. Int J Mol Sci. 2020); 21(7): 2370.
40. Desmet KD, Paz DA, Corry JJ, Eells JT, Wong-Riley MT, Henry MM, et al. Clinical and experimental applications of NIR-LED photobiomodulation. Photomed Laser Surg. 2006; 24(2): 121-128.
41. Kaur C, Foulds WS, Ling EA. Blood-retinal barrier in hypoxic ischaemic conditions: basic concepts, clinical features and management. Prog Retin Eye Res. 2008; 27(6):622-47.
42. Begum R, Powner MB, Hudson N, Hogg C, Jeffery G. Treatment with 670 nm light upregulates cytochrome C oxidase expression and reduces inflammation in an age-related macular degeneration model. PloS One. 2013; 8: e57828.
43. Kaynezhad P, Tachtsidis I, Jeffery G. Optical monitoring of retinal respiration in real time: 670 nm light increases the redox state of mitochondria. Exp Eye Res. 2016;152: 88e93.
44. Calaza KC, Kam JH, Hogg C, Jeffery G. Mitochondrial decline precedes phenotype development in the complement factor H mouse model of retinal degeneration but can be corrected by near infrared light. 2015. Neurobiol Aging. 36:2869e2876.
45. Gkotsi D, Begum R, Salt T, Lascaratos G, Hogg C, Chau KY, et al. Recharging mitochondrial batteries in old eyes. Near infra-red increases ATP. Exp Eye Res. 2014;122: 50e53.
46. Kokkinopoulos I, Colman A, Hogg C, Heckenlively J, Jeffery G. Age-related retinal inflammation is reduced by 670 nm light via increased mitochondrial membrane potential. Neurobiol aging. 2013; 34: 602e609.
2. Vincent JL, Taccone FS, He X. Harmful effects of hyperoxia in postcardiac arrest, sepsis, traumatic brain injury, or stroke: the importance of individualized oxygen therapy in critically ill patients. Can Respir J. 2017; 2017: 2834956. doi: 10.1155/2017/2834956
3. Zaher TE, Mille E J, Morrow DM, Javdan M, Mantell L L.. Hyperoxia-induced signal transduction pathways in pulmonary epithelial cells. Free Radic Biol Med. 2007; 42(7): 897-908.
4. Lajko M, Cardona HJ, Taylor J M, Shah RS, Farrow K N, Fawzi AA. Hyperoxia-induced proliferative retinopathy: early interruption of retinal vascular development with severe and irreversible neurovascular disruption. PLoS One. 2016; 11(11), e0166886.
5. Zhu Y, Park TS, Gidday JM. Mechanism of hyperoxia-induced reduction in retinal blood flow in newborn pig. Exp Eye Res. 1998; 67(3): 357-369.
6. Jean-Louis S, Lovasik J V, Kergoat H. Systemic hyperoxia and retinal vasomotor responses. Invest Ophthalmol Vis Sci. 2005; 46(5):1714-1720.
7. Albarracin R, Eells J, Valter K. Photobiomodulation protects the retina from light-induced photoreceptor degeneration. Invest Ophthalmol Vis Sci. 2011; 52:3582–3592.
8. Albarracin R, Natoli R, Rutar M, Valter K, Provis J. 670 nm light mitigates oxygen-induced degeneration in C57BL/6J mouse retina. BMC neuroscience. 2013; 14(1), https://doi.org/10.1186/1471-2202-14-125.
9. Wong-Riley MT, Liang HL, Eells JT, Chance B, Henry MM, Buchmann E, Kane M, Whelan HT. Photobiomodulation directly benefits primary neurons functionally inactivated by toxins: role of cytochrome c oxidase. J Biol Chem. 2005; 280: 4761e4771.
10. Oron U, Yaakobi T, Oron A, Mordechovitz D, Shofti R, Hayam G, et al. Low-energy laser irradiation reduces formation of scar tissue after myocardial infarction in rats and dogs. Circulation. 2001; 103(2):296–301.
11. Simunovic Z, Ivankovich A, Depolo A. Wound healing of animal and human body sport and traffic accident injuries using low-level laser therapy treatment: a randomized clinical study of seventy-four patients with control group. J Clin Laser Med Surg, 2000; 18(2):67–73.
12. Muili KA, Gopalakrishnan S, Meyer SL, Eells JT, Lyons JA. Amelioration of experimental autoimmune encephalomyelitis in C57BL/6 mice by photobiomodulation induced by 670 nm light. PLoS One. 2012;7(1):24.
13. Abrahan CE, Insua MF, Politi LE, German, OL, Rotstein NP. Oxidative stress promotes proliferation and dedifferentiation of retina glial cells in vitro. J Neurosci Res. 2009; 87: 964e977.
14. Geneva II. Photobiomodulation for the treatment of retinal diseases: a review.Int. J. Ophthalmol. 2016; 9: 145e152.
15. Eells JT, Henry MM, Summerfelt P, Wong-Riley MT, Buchmann EV, Kane M, Whelan NT, Whelan HT. Therapeutic photobiomodulation for methanol-induced retinal toxicity. Proc Natl Acad Sci USA. 2003;100(6):3439–3444.
16. Eells JT, Desmet KD, Kirk DK, Wong-Riley M, Whelan HT, Ver Hoeve J, et al. Photobiomodulation for the treatment of retinal injury and retinal degenerative diseases. In: Proceedings of light-activated tissue regeneration and therapy conference. eds R.W. Waynant and D.B. Tata pp 39-51, Springer, New York, 2008.
17. Qu C, Cao W, Fan Y, Lin Y. Near-infrared light protect the photoreceptor from light-induced damage in rats. Adv Exp Med Biol, 2010; 664:365–374.
18. Tang J, Du Y, Lee CA, Talahalli R, Eells JT, Kern TS. Low-intensity far-red light inhibits early lesions that contribute to diabetic retinopathy: in vivo and in vitro. Invest Ophthalmol Vis Sci. 2013; 54: 3681e3690.
19. Whitcup SM, Nussenblatt RB, Lightman SL, Hollander DA. Inflammation in retinal disease. Int J Inflamm. 2013;724648. doi: 10.1155/2013/724648.
20. Lowry OH, Rosebrough N J, Farr A l, Randall R J. Protein measurements with the folin phenol reagent. J Biol Chem. 1951; 193:265-75.
21. Laemmli UK. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature. 1970; 2777: 680-5.
22. Koracevic D, Koracevic G., Djordjevic V, Andrejevic S and Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol. 2001; 54: 356-361.
23. Aebi H. Catalase in vitro. Methods Enzymol. 1984;105,121–126. http://dx.doi.org/10.1016/S0076-6879(84)05016-3
24. Martinez R, Quintana K, Navarro R, Martin C, Hernandes M L, Aurrekoetxea I, et al. Pro-oxidation and antioxidant potential of catecholestrogens against ferryl-myoglobin-induced oxidative stress. Biochem Biophys Acta. 2002; 1583: 167–175.
25. Hardy P, Beauchamp M , Sennlaub F, Gobeil F Jr , Tremblay L, Mwaikambo B, et al. New insights into the retinal circulation: Inflammatory lipid mediators in ischemic retinopathy. Prostag Leukotr Ess. 2005;72: 301–325.
26. Terraneo L, Virgili E, Caretti A, Bianciardi P, Smaja M. In vivo hyperoxia induces hypoxia- inducible factor-1α overexpression in LNCaP tumor without affecting the tumor growth rate. Int J Biochem Cell biol. 2004; 51: 65-74.
27. Nita M, Grzybowski A. The role of the reactive oxygen species and oxidative stress in the pathomechanism of the age-related ocular diseases and other pathologies of the anterior and posterior eye segments in adults. Oxid Med Cellular Longev. 2016; 2016:3164734. doi: 10.1155/2016/3164734.
28. Penn JS, Henry MM, Tolman BL. Exposure to Alternating Hypoxia and Hyperoxia Causes Severe Proliferative Retinopathy in the Newborn Rat. Pediatr Res. 1994;36(6):724–31.
29. Yanik M, Erel O, Kati M. The relationship between potency of oxidative stress and severity of depression. Acta Neuropsychiatr. 2004; 16: 200-203.
30. Harma M, Harma M, Erel O. Oxidative stress in women with preeclampsia. Am J Obstet Gynecol. 2005; 192: 656–657.
31. Yeni E, Gulum M, Selek S, Erel O, Unal D, Verit A, et al. Comparison of oxidative/antioxidative status of penile corpus cavernosum blood and peripheral venous blood. Int J Impot Res. 2005; 17: 19-22.
32. Graziosi A, Perrotta M , Russo D, Gasparroni G, D’Egidio C, Marinelli B , et al. Oxidative stress markers and the retinopathy of prematurity. J Clin Med. 2020; 9(9): 2711.
33. Liu W, Chen Q, Liu J , Liu KJ. Normobaric hyperoxia protects the blood brain barrier through inhibiting Nox2 containing NADPH oxidase in ischemic stroke. Medical Gas Res. 2011; 1(22):1-8.
34. Demirbag R, Gur M, Yilmaz R, Kunt AS, Erel O, Andac MH. Influence of oxidative stress on the development of collateral circulation in total coronary occlusions. Int J Cardiol. 2007; 116: 14-19.
35. Rabus M, Demirbağ R, Sezen Y, Konukoğlu O, Yildiz A, Erel Ö, et al. Plasma and tissue oxidative stress index in patients with rheumatic and degenerative heart valve disease. Arch Turk Soc Cardiol. 2008; 36: 536-540.
36. Shan Y, Ye X, Xin H. Effect of the grape seed proanthocyanicin extract on the free radical and energy metabolism indicators during the movement. Sci Res Essay. 2010; 5:148-153.
37. Bowers, F, Valter K, Chan S, Walsh N, Maslim J, Stone J. Effects of oxygen and bFGF on the vulnerability of photoreceptors to light damage. Invest Ophthalmol Vis Sci. 2001; 42(3): 804-815.
38. Dorfman A, Dembinska O, Chemtob S, Lachapelle P. Early manifestations of postnatal hyperoxia on the retinal structure and function of the neonatal rat. Invest Ophthalmol Vis Sci. 2008; 49(1): 458-466.
39. Heinig N, Schumann U, Calzia, D, Panfoli I, Ader M, Schmidt M H, et al. Photobiomodulation Mediates Neuroprotection against Blue Light Induced Retinal Photoreceptor degeneration. Int J Mol Sci. 2020); 21(7): 2370.
40. Desmet KD, Paz DA, Corry JJ, Eells JT, Wong-Riley MT, Henry MM, et al. Clinical and experimental applications of NIR-LED photobiomodulation. Photomed Laser Surg. 2006; 24(2): 121-128.
41. Kaur C, Foulds WS, Ling EA. Blood-retinal barrier in hypoxic ischaemic conditions: basic concepts, clinical features and management. Prog Retin Eye Res. 2008; 27(6):622-47.
42. Begum R, Powner MB, Hudson N, Hogg C, Jeffery G. Treatment with 670 nm light upregulates cytochrome C oxidase expression and reduces inflammation in an age-related macular degeneration model. PloS One. 2013; 8: e57828.
43. Kaynezhad P, Tachtsidis I, Jeffery G. Optical monitoring of retinal respiration in real time: 670 nm light increases the redox state of mitochondria. Exp Eye Res. 2016;152: 88e93.
44. Calaza KC, Kam JH, Hogg C, Jeffery G. Mitochondrial decline precedes phenotype development in the complement factor H mouse model of retinal degeneration but can be corrected by near infrared light. 2015. Neurobiol Aging. 36:2869e2876.
45. Gkotsi D, Begum R, Salt T, Lascaratos G, Hogg C, Chau KY, et al. Recharging mitochondrial batteries in old eyes. Near infra-red increases ATP. Exp Eye Res. 2014;122: 50e53.
46. Kokkinopoulos I, Colman A, Hogg C, Heckenlively J, Jeffery G. Age-related retinal inflammation is reduced by 670 nm light via increased mitochondrial membrane potential. Neurobiol aging. 2013; 34: 602e609.
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