Antibacterial Effects of a 940 nm Diode Laser With/ Without Silver Nanoparticles Against Enterococcus faecalis Antibacterial Effects of Diode Laser and Silver Nanoparticles
Journal of Lasers in Medical Sciences,
Vol. 12 (2021),
13 February 2021
,
Page e73
Abstract
Introduction: The final goal of root canal therapy is to remove most bacteria from the root canal. This study aimed at comparing the antibacterial effects of a diode laser with a wavelength of 940nm and silver nanoparticles and the synergic effects of both techniques on Enterococcus faecalis.
Methods: Ninety single-rooted human teeth were decoronated and prepared with rotary files. The samples were irrigated with sodium hypochlorite and EDTA17%. Then they were autoclaved and contaminated with E. faecalis suspension (1.5 × 108 CFU/mL) for 21 days. The samples were fixed in a microtube and were randomly divided into 4 experimental (n=20) groups and a negative control group (n=10) as follows: Group 1: hypochlorite sodium 5%, Group 2: silver nanoparticle, Group 3: diode laser, and Group 4: diode laser and silver nanoparticle. The samples were obtained from dentin chips before and after the intervention. The data were analysed using the Kruskal-Wallis nonparametric test. Furthermore, alterations in bacterial colonies were entered using the Wilcoxon signed ranks test (α=0.05).
Results: There was a significant decrease in colony counts for all groups after interventions (P value<0.05). Also, all groups showed more reductions in colony counts compared with the negative control group (P-value <0.004). There was a significant reduction for group 1 in comparison with other groups (P-value <0.001) and this group had an extreme decrease of colony counts (RCC=100%). There was an important differential between silver nanoparticles and diode laser groups in bacterial counts (P value<0.001) and silver nanoparticles (RCC=83.15%) had more efficiency than the diode laser (RCC=41/33%). RCC of group 4 was 68/52%.
Conclusion: Followed by sodium hypochlorite 5%, silver nanoparticles were the most effective antibacterial substances. The 940 nm laser diode had less antibacterial effect compared to its use with silver nanoparticles.
- Root Canal Therapy, Laser Diode, Silver Nanoparticles, Hypochlorite Sodium, Enterococcus faecalis
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References
Naghavi N, Rouhani A, Irani S, Naghavi N, Banihashemi E. Diode Laser and Calcium Hydroxide for Elimination
of Enterococcus Faecalis in Root Canal. J Dent Mater and Tech. 2014;3(2):55-60. Doi: 10.22038/JDMT.2014.2436.
Asnaashari M, Godiny M, Azari-Marhabi S, Tabatabaei FS, Barati M. Comparison of the antibacterial effect of 810 nm diode laser and photodynamic therapy in reducing the microbial flora of root canal in endodontic retreatment in patients with periradicular lesions. J Lasers Med Sci. 2016;7(2):99-104. Doi: 10.15171/jlms.2016.17
Garcez AS, Ribeiro MS, Tegos GP, Núñez SC, Jorge AO, Hamblin MR. Antimicrobial photodynamic therapy
combined with conventional endodontic treatment to eliminate root canal biofilm infection. Lasers Surg Med.
;39(1):59-66. Doi:10.1002/lsm.20415.
Shaktawat AS, Verma KG, Goyal V, Jasuja P, Sukhija SJ, Mathur A. Antimicrobial efficacy of 980 nm diode laser
on Enterococcus feacalis in conjunction with various irrigation regimes in infected root canals: An in vitro study. J Indian Soc Pedod Prev Dent. 2018;36(4):347-351. Doi: 10.4103/JISPPD.JISPPD_128_17.
Lane J, Bonsor S. Survival rates of teeth treated with bacterial photo-dynamic therapy during disinfection
of the root canal system. Br Dent J. 2019;226(5):333-9. Doi:10.1038/s41415-019-0026-z.
González-Luna IV P, Martínez-Castañón G-A, ZavalaAlonso N-V, Patiño-Marin N, Niño-Martínez N, MoránMartínez J, et al. Bactericide effect of silver nanoparticles as a final irrigation agent in endodontics on Enterococcus faecalis: an ex vivo study. J Nanomaterials 2016;2016:e 7597295. Doi: org/10.1155/2016/7597295.
Afkhami F, Akbari S, Chiniforush N. Entrococcus faecalis elimination in root canals using silver nanoparticles,
photodynamic therapy, diode laser, or laser-activated nanoparticles: an in vitro study. J Endod. 2017;43(2):279-
Doi:10.1016/j.joen.2016.08.029.
Verma N, Sangwan P, Tewari S, Duhan J. Effect of different concentrations of sodium hypochlorite on
outcome of primary root canal treatment: a randomized controlled trial. J Endod. 2019;45(4):357-63. Doi:10.1016/j. joen.2019.01.003.
Hongo T, Watanabe S, Yao K, Satake K, Okiji T. Evaluation of cleaning efficacy-related properties of root canal
irrigant activation using a computer-controlled hot tip powered with a diode laser. Asian Pac J Dent. 2019;19:9-15.
Doi:ndljp/pid/11606300.
Abdelgawad LM, Asmail N, Latif SA, Safaan AM. Efficacy of diode laser and sonic agitation of Chlorhexidine and Silver-nanoparticles in infected root canals. Braz Dent Sci. 2020;23(3):1-9. DOI: 10.14295/bds.2020.v23i3.1989
Shehab NF, Zakaria NA, Taha MY. Efficiency of Diode Laser as Root Canal Disinfectant Against Enterococcus faecalis: An in Vitro Study. Int J Sci Applied Res. 2018;42(5):15-23.
Walia V, Goswami M, Mishra S, Walia N, Sahay D. Comparative Evaluation of the Efficacy of Chlorhexidine,
Sodium Hypochlorite, the Diode Laser and Saline in Reducing the Microbial Count in Primary Teeth Root
Canals–An In Vivo Study. J Lasers Med Sci. 2019;10(4):268-74. Doi: 10.15171/jlms.2019.44
Hegde MN, Bhat R, Shetty P. Efficiency of a semiconductor diode laser in disinfection of the root canal system in endodontics: An in vitro study. J Int Clin Dent Res Org. 2015;7(1):35-8. Doi:10.4103/2231-0754.153493
Mehrvarzfar P, Saghiri MA, Asatourian A, Fekrazad R, Karamifar K, Eslami G, et al. Additive effect of a diode
laser on the antibacterial activity of 2.5% NaOCl, 2% CHX and MTAD against Enterococcus faecalis contaminating
root canals: an in vitro study. J Oral Sci. 2011;53(3):355-60. Doi:10.2334/josnusd.53.355.
Habibipour R, Moradi-Haghgou L, Farmany A. Green synthesis of AgNPs@ PPE and its Pseudomonas aeruginosa biofilm formation activity compared to pomegranate peel extract. Int J Nanomedicine. 2019 Aug 28; 14:6891-6899. doi: 10.2147/IJN.S209912. eCollection 2019
Castelo BP, Martin BB, Ruiz PM, Rivas MB, Bahillo J, Perez EA, et al. Combined sodium hypochlorite and 940 nm diode laser treatment against mature E. faecalis biofilms in-vitro. J Lasres Med Sci. 2012; 3(3):116-121.
Abbott P, Yu C. A clinical classification of the status of the pulp and the root canal system. Aust Dent J.
(S1);52:S17-S31. doi: 10.1111/j.1834-7819.2007. tb00522.x.
Fonseca MB, Júnior POT, Pallota RC, Filho HF, Denardin OVP, Rapoport A, et al. Photodynamic therapy for root
canals infected with Enterococcus faecalis. Photomed Laser Surg. 2008;26(3):209-13. Doi: 10.1089/pho.2007.2124.
Gutknecht N, Van Gogswaardt D, Conrads G, Apel C, Schubert C, Lampert F. Diode laser radiation and its
bactericidal effect in root canal wall dentin. J Clin Laser Med Surg. 2000;18(2):57-60. Doi: 10.1089/clm.2000.18.57.
Rodrigues CT, De Andrade F, De Vasconcelos L, Midena R, Pereira T, Kuga M, et al. Antibacterial properties of silver nanoparticles as a root canal irrigant against Enterococcus faecalis biofilm and infected dentinal tubules. Int Endod J. 2018;51(8):901-11. Doi: 10.1111/iej.12904
Moghadas L, Shahmoradi M, Narimani T. Antimicrobial activity of a new nanobased endodontic irrigation solution:In vitro study. Dent Hypotheses. 2012;3(4):142-146 Doi:10.4103/2155-8213.106838.
Litvin VA, Minaev BF. Spectroscopy study of silver nanoparticles fabrication using synthetic humic substances
and their antimicrobial activity. Spectrochim Acta A Mol Biomol Spectrosc. 2013;108:115-22. DOI: 10.1016/j.
saa.2013.01.049.
Maneerung T, Tokura S, Rujiravanit R. Impregnation of silver nanoparticles into bacterial cellulose for antimicrobial wound dressing. Carbohydr Polym. 2008;72(1):43-51. Doi: org/10.1016/j.carbpol.2007.07.025.
Ashofteh K, Sohrabi K, Iranparvar K, Chiniforush N. In vitro comparison of the antibacterial effect of three intracanal irrigants and diode laser on root canals infected with Enterococcus faecalis. Iran J Microbiol. 204;6(1):26-30.
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