Antifungal Activity of Topical Microemulsion Containing Ziziphus spina-christi L. for the Treatment of Fungal Vaginitis Antifungal Activity of Microemulsion Containing Ziziphus spinachristi L
Iranian Journal of Pharmaceutical Sciences,
Vol. 17 No. 1 (2021),
15 January 2021
,
Page 43-50
https://doi.org/10.22037/ijps.v17.40298
Abstract
Vulvovaginal candidiasis (VVC) caused by Candida albicans and other Candida species is a high-risk event in patients admitted to hospital. Ziziphus genus is a rich source of medicinal compounds, effective for the treatment or prevention of some diseases. This study aimed to evaluate the antifungal effect of topical microemulsion containing Ziziphus spina-Christi L (ZSC) extracts against Candida spp. Z. Spina-Christi was collected and extracted in a Soxhlet apparatus using the ethanol and methanol solvents. Microemulsions were formulated by the composition of surfactant (Tween 80) along with cosurfactant (propylene glycol), oil, and water and were characterized regarding their stability. The The in vitro antifungal study was performed by measuring and comparing the diameter of zones of inhibition (in mm) against C. albicans (ATCC 3153), C. parapsilosis (ATCC 2195) and C. krusei (ATCC 573) using agar well diffusion test, for the various formulations (without extract and containing ZSC extract). The optimal microemulsion was analyzed for the average diameter of droplets, pH, viscosity and zeta potential. The microemulsion containing Ziziphus spina-Christi ethanolic extract had a significant inhibitory effect on the different species of Candida, but the most inhibitory effect was found against C. albicans. The result showed that the ethanolic extract in microemulsion was Light green and, average globule size was 2.93 mm, no separation after centrifugation at 3000 rpm for 30 min, pH at 3.8, and viscosity was 8.523cps. The results of this study indicate that the ethanolic extract has interesting antifungal properties and can used for the treatment of fungal infections. More research is required to check this plant performance to treat the patients with Candidiasis.
- Antifungal activity
- Ziziphus spina-Christi L
- Candida spp.
- Candidiasis
- Microemulsion
- Extract
How to Cite
References
[2] Zarei Mahmoudabadi A, Rezaei-Matehkolaei A, Navid M, Torabizadeh M, Mazdarani S. Colonization and antifungals susceptibility patterns of Candida species isolated from hospitalized patients in ICUs and NICUs. J. nephropathol. (2015) 4:77-84.
[3] Raesi Vanani A, Mahdavinia M, Kalantari H, Khoshnood S, Shirani M. Antifungal effect of the effect of Securigera securidaca L. vaginal gel on Candida species. Curr. Med. Mycolgy (2019) 5:31-5.
[4] Sojakova M, Liptajova D, Borovsky M, Subik J. Fluconazole and itraconazole susceptibility of vaginal yeast isolates from Slovakia. Mycopathologia (2004) 157:163-9.
[5] Shirani K, Hassani FV, Razavi-Azarkhiavi K, Heidari S, Zanjani BR, Karimi G. Phytotrapy of cyclophosphamide-induced immunosuppression. Environ. Toxicol. Pharmacol. (2015) 39:1262-75.
[6] Ramesh P, Okigbo R. Effects of plants and medicinal plant combinations as anti-infectives. Afr. J. Pharm. Pharmacol. (2008) 2:130-5.
[7] Shirani M, Samimi A, Kalantari H, Madani M, Kord Zanganeh A. Chemical composition and antifungal effect of hydroalcoholic extract of Allium tripedale (Tvautv.) against Candida species. Curr. Med. Mycolgy (2017);3(1):6-12.
[8] Abad MJ, Ansuategui M, Bermejo P. Active antifungal substances from natural sources. Arkivoc. (2007) 7:116-45.
[9] Zomorodian K, Saadat F, Zaini F, Tarazooie B, Rezaie S. Inhibitory effect of Zizyphus extract on fungal infection. Iran J. Pharm. Res. (2010) 3:81-82.
[10] Asgarpanah J, E H. Phytochemistry and pharmacologic properties of Ziziphus spina christi (L.) Willd. AFR. J. Pharm. Pharmaco. (2012) 6: 2332-2339.
[11] Abdallah E. Antibacterial Activity of Fruit Methanol Extract of Ziziphus spina-christi from Sudan. Int. J. Curr. Microbiol. Appl. Sci. (2017) 6: 38-44.
[12] Guimarães GP, Reis MYdFA, Silva DTCd, Mendonça Junior FJB, Converti A, Pessoa Jr A, et al. Antifungal activity of topical microemulsion containing a thiophene derivative. Braz. J. Microbiol. (2014) 45:545-550.
[13] Kaewbanjong J, Wan Sia Heng P, Boonme P. Clotrimazole microemulsion and microemulsion-based gel: evaluation of buccal drug delivery and irritancy using chick chorioallantoic membrane as the model. J. Pharm. Pharmacol. (2017) 69:1716-23.
[14] Patel D, Patel N, Patel A, Patel J, Patel A, Patel M. Microemulsion as a Novel Carrier for Vaginal Delivery-A Review. Research J. Pharm. and Tech. (2012) 5:877-882.
[15] Johal HS, Garg T, Rath G, Goyal AK. Advanced topical drug delivery system for the management of vaginal candidiasis. Drug Deliv. (2016) 23:550-563.
[16] Sharma AK, Garg T, Goyal AK, Rath G. Role of microemuslsions in advanced drug delivery. Artif. Cells Nanomed. Biotechnol. (2016) 44:1177-1185.
[17] Wang J, Wang Y, Wang Z, Wang F, He J, Yang X, et al. A thermosensitive gel based on w1/o/w2 multiple microemulsions for the vaginal delivery of small nucleic acid. Drug Deliv. (2019) 26:168-178.
[18] Parveen R, Akhtar N, Mahmood T. Topical microemulsion containing Punica granatum extract: its control over skin erythema and melanin in healthy Asian subjects. Postepy. Dermatol. Alergol. (2014) 31:351-355.
[19] Akram A, Rasul A, Waqas MK, Irfan M, Khalid SH, Aamir MN, et al. Development, characterization and evaluation of in-vitro anti-inflammatory activity of ginger extract based micro emulsion. Pak. J. Pharm. Sci. (2019) 32:1327-1332.
[20] Akram A, Akhtar N, Waqas MK, Rasul A, Rehman KU, Khan J, et al. Development, characterization and evaluation of ginger extract loaded microemulsion: In vitro and Ex vivo release studies. Pak. J. Pharm. Sci. (2019) 32:1873-1877.
[21] Maqsood I, Masood MI, Bashir S, Nawaz HM, Anjum AA, Shahzadi I, et al. Preparation and in vitro evaluation of Nystatin micro emulsion based gel. Pak. J. Pharm. Sci. (2015) 28:1587-1593.
[22] Moghimipour E, Salimi A, Eftekhari S. Design and characterization of microemulsion systems for naproxen. Adv. Pharm. Bull. (2013) 3:63-71.
[23] Sadeghi Nejad B, Rajabi M, Zarei Mamoudabadi A, Zarrin M. In Vitro Anti-Candida Activity of the Hydroalcoholic Extracts of Heracleum persicum Fruit Against Phatogenic Candida Species. Jundishapur J. Microbiol. (2014) 7:e8703.
[24] Magaldi S, Mata-Essayag S, Hartung de Capriles C, Perez C, Colella MT, Olaizola C. Well diffusion for antifungal susceptibility testing. Int. J. Infect. Dis. (2004) 8:39-45.
[25] Kim HJ, Suh H-J, Lee CH, Kim JH, Kang SC, Park S, et al. Antifungal activity of glyceollins isolated from soybean elicited with Aspergillus sojae. J. Agr. Food Chem. (2010) 58:9483-94837.
[26] Mohammed G, Abdulrahman FI, Khan IZ, Hussaini MI, Muazu J, Si Y. Antimicrobial efficacies of ethanolic extract and active column fractions of the stem-bark of Zizyphus spina-christi L. (Desf). Int. J. Pharm (2013) 5: 455-460.
[27] Mardani M, Badiee P, Gharibnavaz M, Jassebi A, Jafarian H, Ghassemi F. Comparison of anti-Candida activities of the ancient plants Lawsonia inermis and Ziziphus spina christi with antifungal drugs in Candida species isolated from oral cavity. J. Conserv. Dent. (2018) 21:359-362.
[28] Beatriz P-M, Ezequiel V-V, Pilar C-R. Antifungal activity of Psidium guajava organic extracts against dermatophytic fungi. J. Med. Plants. Res. (2012) 6:5435-5438.
[29] Nesseem DI, Michel CG, Sleem AA, El-Alfy TS. Formulation and evaluation of antihyperglycemic leaf extracts of Zizyphus spina-christi (L.) Willd. Pharmazie (2009) 64:104-109.
[30] Kaleem WA, Muhammad N, Khan H, Rauf. Pharmacological and phytochemical studies of genus Zizyphus. Middle East J. Sci. Res. (2014) 21:1243-1263.
[31] Almeer RS, El-Khadragy MF, Abdelhabib S, Abdel Moneim AE. Ziziphus spina-christi leaf extract ameliorates schistosomiasis liver granuloma, fibrosis, and oxidative stress through downregulation of fibrinogenic signaling in mice. Plos. One. (2018) 13:e0204923.
[32] M Nazif N. Phytoconstituents of Zizyphus spina-Christ L. fruits and their antimicrobial activity. Food Chem. (2002) 76: 77-81.
[33] el-Din Hussein Mahran G, Glombitza KW, Mirhom YW, Hartmann R, Michel CG. Novel Saponins from Zizyphus spina-christi growing in Egypt. Planta. Med. (1996) 62:163-165.
[34] Braga PC, Culici M, Alfieri M, Dal Sasso M. Thymol inhibits Candida albicans biofilm formation and mature biofilm. Int. J. Antimicrob. Agents (2008) 31:472-477.
[35] Dalleau S, Cateau E, Bergès T, Berjeaud J-M, Imbert C. In vitro activity of terpenes against Candida biofilms. Int. J. Antimicrob. Agents (2008) 31:572-576.
[36] Al-Ali S, Al-Judaibi A. Biochemical and Molecular Effects of Phoenix dactylifera and Ziziphus spina-christi Extracts on Candida albicans. J. Biosciences and Medicines (2019) 7:29-43.
[37] Puranajoti P, Patil R, Sheth P, Bommareddy G, Dondeti P, Egbaria K. Design and development of topical microemulsion for poorly water-soluble antifungal agents. J. Appl. Res. (2002) 2:27-28.
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