Evaluation of Cytotoxic and Antioxidant Activity and Total Phenolic Content of Some Soft Corals from the Persian Gulf Cytotoxic, Antioxidant Activity and Total Phenolic Content of Persian Gulf Soft Corals
Iranian Journal of Pharmaceutical Sciences,
Vol. 16 No. 3 (2020),
1 July 2020
,
Page 1-10
https://doi.org/10.22037/ijps.v16.40385
Abstract
Marine soft corals contain a variety of secondary metabolites with diverse, biological activities, including cytotoxic and anti-oxidant. The aim of this study was to evaluate the total phenolic content, antioxidant and cytotoxic activities of some soft corals of Persian Gulf including: Junceella juncea, Cavernularia sp., white Menella sp., brown Menella sp., Virgularia sp., Sinularia compressa, Sinularia variablis and Sinularia
polydactyla were collected from Persian Gulf and extracted by maceration with methanol-ethyl acetate (1:1) solvent. The extract was evaporated and the total phenolic content was evaluated by using the Folin-ciocalteu reagent. The antioxidant activity of corals was tested by using DPPH photometric assay and cytotoxic activity of them against MCF-7 and OVCAR-3 cancer cell lines were performed. The cell survivals of MCF-7 and OVCAR-3 cell lines were decreased by increasing the concentration of the extracts. The brown Menella sp. showed the highest cytotoxic activity against MCF-7 with IC50 values of 325.45±2.57 μg/ml and S. polydactyla showed the highest cytotoxic activity against OVCAR-3 cell line with IC50 values of 260.99±7.93 μg/ml. White Menella sp. showed the highest antioxidant activity with IC50 values 0.056 μg/ml. Finally Cavernularia sp. had the most polyphenol content with 186.33 mg/L. Key words: soft corals, antioxidant DPPH photometric assay, total phenolic content, Folin–Ciocalteu method, cytotoxic activity, Persian Gulf.
- soft corals
- antioxidant DPPH photometric assay
- total phenolic content
- Folin–Ciocalteu method
- cytotoxic activity
- Persian Gulf
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