ISSN: 1735-1308

2019

REVIEW


Association of Transforming Growth Factor-Beta1 rs1982073 Polymorphism with Susceptibility to Acute Renal Rejection: a Systematic Review and Meta-Analysis

Farzaneh Najafi, Seyed Alireza Dastgheib, Jamal Jafari-Nedooshan, Mansour Moghimi, Naeimeh Heiranizadeh, Mohammad Zare, Elham Salehi, Hossein Neamatzadeh

Urology Journal, 2019, 5 January 2019
https://doi.org/10.22037/uj.v0i0.5437

Purpose: The association of rs1982073 (codon 10) polymorphism at Transforming Growth Factor- ?1 (TGF-?1) gene with acute renal rejection (ARR) has been reported by several studies. However, the results were controversial. To derive a more precise estimation of this association, a meta-analysis was performed.

Methods: The eligible literatures were identified through PubMed, Scopus, Web of Science, EMBASE, SciELO, WanFang, and CNKI databases up to July 01, 2019. The pooled odds ratios (ORs) with corresponding 95% confidence intervals (CIs) were used to calculate the strength of the association.

Results: A total of 23 case-control studies with 795 ARR cases and 1,562 non-AR controls were selected. Pooled data revealed that there was no significant association between TGF-Beta1 codon 10 polymorphism and an increased risk of ARR in the overall population (C vs. T: OR=0.908, 95% CI 0.750-1.099, p=0.322; CT vs. TT: OR=1.074, 95% CI 0.869-1.328, p=0.507; CC vs.TT: OR=0.509, 95% CI=0.738-1.253, p=0.770; CC+CT vs. TT: OR = 0.917, 95% CI 0.756-1.112, p = 0.376, and CC vs. CT+TT: OR=0.995, 95% CI 0.809-1.223, p=0.959). Moreover, stratified analysis revealed no significant association between the TGF-?1 rs1982073 polymorphism and ARR risk by ethnicity and cases type (recipient and donor).

Conclusions: The current meta-analysis demonstrated that the TGF-?1 rs1982073 polymorphism was not significantly associated with increased risk of ARR. However, studies with a larger number of subjects among different ethnic groups are needed to further validate the results.

Purpose To assess the diagnostic accuracy of fluorine-18 fluorodeoxyglucose positron emission tomography combined with the computed tomography (18F-FDG PET/CT) in the detection of recurrent or residual urinary bladder cancer with meta-analysis.

Methods We searched PubMed/MEDLINE, Embase, Web of Science, CBM, CNKI, VIP, and Wanfang databases through October 2019. Two reviewers independently screened the full articles. The imaging findings were confirmed by either histopathology or clinical follow-up. Sensitivity, specificity likelihood ratio and diagnostic odds ratio were pooled with 95 % confidence intervals (CI). Overall test performance was summarized by a summary receiver operating characteristic (ROC) curve. The Meta-DiSc software (version 1.4) was used to perform the meta-analysis.

Results The meta-analysis included 7 studies. The pooled sensitivity and specificity of PET/CT for the detection of recurrent or residual urinary bladder cancer was 94.0% (95% CI: 91.0%–96.0%) and 92.0% (95% CI: 88.0%–95.0%), respectively. Positive likelihood ratio, negative likelihood ratio and diagnostic odds ratio were 9.77 (95% CI: 4.91–19.41), 0.99(95% CI: 0.06–0.13) and 95.09 (95% CI: 47.96–188.53), respectively. When residual urinary bladder cancer was excluded, sensitivity changed slightly.

Conclusion This meta-analysis suggested that the diagnostic accuracy of PET/CT was good in detecting recurrent or residual urinary bladder cancer.

Purpose: To evaluate the evidence that could help health system policy makers to approach the preference for same-gender urologists.

Methods: We performed this systematic narrative review according to the PRISMA guidelines. We searched MEDLINE, Web of science, EMBASE, CINAHL and Google Scholar for articles focused on the  preference for patient-urologist gender similarity in the male-dominated department from 1999-2019. Finally, a narrative synthesis of studies meeting the inclusion and quality criteria was conducted in accordance with the nature of the evidences. We applied a thematic analysis using inductive approach for synthesizing studies employing heterogeneous research methods and designs.

 

Results: Of 208 titles and abstracts screened, 23 were included and three major themes including Reasons, Impacts, and Implications were identified. Overall, patients with urologic problems prefer same gender urologists and females were more likely to prefer the same gender urologist than males. Many women delays care due to a perceived lack of female urologists. The major identified reasons for the same-gender preference are religious believe, cultural background, emotional relationship, past experiences, and sensitive examinations. The results indicate that the urologists-patients gender similarity improves the quality of primary care. Our review reveled that Urologists prefer to perform more same gender-specific procedures. Beside, Female urologist and residents perceived to underestimate from their male counterparts.

 

Conclusion: This study could help health system to honor the patient’s preference for same gender urologist. The findings may help medical education and health policy makers to move the male-dominated urology departments towards a culture supportive of female urologists.

OBJECTIVE:


To evaluate the impact of diabetes mellitus (DM) on the recovery of urinary continence (UC) after radical prostatectomy (RP).


METHODS:


Trials were identified in a literature search of PubMed, Embase, Cochrane Library and Web of Science using appropriate search terms. All comparative studies reporting diabetes mellitus, study characteristics, and outcome data including the relationship between diabetes mellitus and urinary continence data were included. Continence rates at different time after RP were compared. Odds ratio (OR) was used for the comparison and all the results were presented with 95% confidence intervals (CIs).


RESULTS:


7 cohort studies comprising with 5944 participants were included, the percentage of DM patients was 8.7%. The results showed that DM increased urinary incontinence risk at 12 months after RP (OR 0.54, 95%CI 0.36 to 0.81, ????=0.003). The continence rates were not significantly different between DM and Non-DM groups at short-term (catheter removal, 3 months, 6 months) and long-term (>12 months). When stratified by the surgical methods, the pooled results showed similar results in patients who underwent robot -assisted radical prostatectomy (RARP).


CONCLUSION:


DM has an adverse impact on the recovery of UC during the intermediate-term after RP. Well-designed trials with strict control of confounders are needed to make results more comparable.


Purpose: We aimed to compare the safety and efficacy between laparoscopic transperitoneal ureterolithotomy (LTU) and laparoscopic retroperitoneal ureterolithotomy (LRU) in the treatment of large (>10mm) and proximal ureteral stones.


Materials and Methods: Electronic databases, including PubMed, EMBASE, Cochrane Library, Web of Science, and Scopus searched through December 2019. Comparative studies were comparing two approaches included. The primary outcome was a single-procedure success rate; the secondary outcomes included operative time, hospital duration, and complications (according to the Clavien-Dindo Grade). Newcastle–Ottawa scale (NOS) and the modified Jadad scale was used to evaluate the quality of the included studies.  The Egger's test estimated publication bias. The meta-analysis was performed by Review Manager 5.3 and STATA 15.0.


Results: 7 studies, involving 125 participants in LTU group and 128 in LRU group, were included in the study. The results suggested that both single-procedure success rate and the rate of postoperative paralytic ileus were significantly higher in LTU group than in LRU group (95.2% vs 87.5%, 95% CI: .00-.16, RD = .08, P = .04; 10.4% vs 0, 95% CI: .02- .19, RD = .10, P = .02, respectively). No publication bias of the primary outcome was observed with the Egger’s test (P = .117). No significant differences were noted in terms of operative time and hospital duration (95% CI: -18.95-8.80, MD = -5.08, P = .47; 95% CI: -.98- .58, MD = -.20, P = .61, respectively). Additionally, according to Clavien-Dindo Grade, the rates of major complications (>= Grade 3a) including open conversion (.8% vs 5.5%, 95%CI: -.11- .01, RD = -.05, P = .12), stone migration (8.1% vs 6.7%, 95% CI: -.08- .11, RD = .02, P = .76), vascular injury (5.4% vs 0, 95%CI: -.03- .14, RD = .05, P = .21) and ureteral stricture (1.3% vs 5.3%, 95% CI: -.11- .02, RD = -.04, P = .20), were comparable between two groups.


Conclusion: In the treatment of large and proximal ureteral calculi, LTU has a significantly higher single-procedure success rate and a higher rate of postoperative paralytic ileus than LRU. However, the complication was well-tolerated. The small sample size and limited, including studies, were the main limitations.


Safety of Surgery in benign Prostatic Hyperplasia Patients on Antiplatelet or Anticoagulant Therapy: A Systematic Review and Meta-Analysis

Liang Xiong Fa, Wu Wei Zhou, Huang Ya Peng, Zhang Shi Ke, Huang Jian, Zeng Tao, Zhong Fang Ling, Lai Yong Chang, Duan Xiao Lu, Cai Chao, Alberto Gurioli, Deng Tuo, Wu Wen Qi

Urology Journal, 2019, 5 January 2019 , Page 5974
https://doi.org/10.22037/uj.v16i7.5974

Purpose: The management strategies of anticoagulant (AC) or antiplatelet (AP) therapy in the preoperative period of benign prostatic hyperplasia (BPH) is still controversial. Therefore, a meta-analysis to systematically evaluate the surgical safety for BPH patients on AC or AP therapy was performed.


Materials and Methods: The protocol for the review is available on PROSPERO (CRD42018105800). A literature search was performed by using MEDLINE, Web of Science, PubMed, Cochrane library and Embase. Summarized odds ratios (OR), mean difference (MD) and 95% confidence intervals (CI) were used to assess the difference in outcomes.


Results: We identified 13 trials with a total of 3767 patients. Intragroup significant difference was found in bleeding complications and blood transfusions when undergoing transurethral resection of the prostate (TURP). For laser surgery, the intragroup significant difference was found in result of blood transfusion. Bridging therapy would not cause higher risk of bleeding complication and blood transfusion during perioperative period. Besides, no difference existed in operation time, catheterization time, hospitalization and thromboembolic events.


Conclusion: Patients with BPH on perioperative AC/AP therapy would have a risk of postoperative hemorrhage after TURP or laser treatments. To reduce the risk of hemorrhage, bridging therapy could be a good choice.

Ramadan Fasting and Kidney Stones: A Systematic Review

Mohsen Amjadi, Farzin Soleimanzadeh, Hamidreza Ghamatzadeh , Sakineh Hajebrahimi, Hossein Hosseinifard, Hanieh Salehi Pourmehr, Fateme Tahmasbi

Urology Journal, 2019, 5 January 2019 , Page 6373
https://doi.org/10.22037/uj.v16i7.6373

Purpose: Ramadan fasting in Muslims may contain several hours of abstaining from food and drinking in any kind. This can potentially increase the risk of urinary stone disease. Current literature on possible effects of Ramadan fasting on urolithiasis is rather limited. Having the gap in scientific background, we decided to evaluate the available comparative information in this systematic review. 


Materials and Methods: We included all studies comparing fasting and non-fasting conditions, studies evaluating stone formation and clinical manifestations of kidney stone disease. All the English studies published from January 1980 to the end of 2019 were included. The exclusion criteria were as followed: fasting out of Ramadan, non-comparative studies, animal studies, patients with bladder stones and studies evaluating conditions that are only indirectly related to the stone formation or clinical manifestations of it. Applying the Joanna Briggs Institute (JBI) methodology for systematic review showed the quality of included studies was not high.


Results: Only five studies remained after exclusion. Meta-analysis was not applicable due to the diversity in methods and evaluated population. 


Conclusion: Main trend of the included studies is toward showing no difference between fasting and non-fasting conditions in terms of renal stone formation. However, generalization of the findings to greater populations should be applied carefully considering the heterogeneity of results and quality of studies.

Purpose: The related research of green-light laser vaporization in the treatment of non-muscle invasive bladder cancer (NMIBC) is limited. This study focused on analyzing the effectiveness and safety of it from the perspective of an extensive literature review.


Methods: A comprehensive search of CNKI, WanFang, VIP, PubMed, Embase, and CENTRAL databases for photoselective vaporization of bladder tumor and transurethral resection of bladder tumor treatment of non-muscle invasive bladder cancer (NMIBC). The search included studies from January 1996 to December 2019. Two reviewers independently screened literature, extracted data, assessed the risk of bias of included studies. RevMan 5.3 software was used for Meta-analysis.


Results: A total of 18 RCTs involving 1648 patients met the predefined criteria. Meta-analysis data demonstrated that the PVBT group exhibited a significant advantage over the TURBT group in intraoperative obturator nerve reflex (RR = 0.09, 95% CI [0.04, 0.18], P< 0.001)and bladder perforation (RR = 0.14, 95% CI [0.07, 0.28], P< 0.001) and postoperative 1-year recurrence (RR = 0.52, 95% CI [0.40, 0.67], P< 0.001). The PVBT procedure has advantages over TURBT in the amount of surgical bleeding (MD = −17.27, 95% CI [−24.73, −9.81], P< 0.001) and the length of hospital stay (MD = −2.80, 95% CI [−3.82, −1.87], P< 0.001), bladder irrigation time (MD = −0.95, 95% CI [−1.49, −0.42], P< 0.001), and catheter indwelling time (MD = −2.60, 95% CI [−3.30, −1.90], P< 0.001). There was no difference between the two types of surgery in the incidence of postoperative urethral stricture (RR = 0.53, 95% CI [0.15, 1.83], P = 0.32) and the length of surgery (MD = −2.46, 95% CI [−5.37, 0.46], P = 0.10).


Conclusion: Our systematic review and meta-analysis suggests that PVBT is better than TURBT as an alternative treatment for patients with NMIBC in safe aspect. However, whether it is equally effective in terms of oncological control remains to be elucidated, and additional high quality RCTs are needed to confirm our findings.

ORIGINAL PAPER (ENDOUROLOGY AND STONE DISEASE)


Purpose: To investigate the safety and efficacy of Single Percutaneous Tract Combined with Flexible Nephroscopy in the Management of 2-4 cm renal calculi.

Materials and Methods: We retrospectively analysed the treatment data of patients with 2-4 cm renal calculi from June 2010 to June 2017. The data included 217 cases of percutaneous nephrolithotomy (PNL), 441 cases of retrograde intrarenal surgery (RIRS) and 217 cases of single-access percutaneous nephrolithotomy combined with flexible nephroscopy (PNCFN). The collected data were analyzed..

Results:

A total of 875 cases were studied, with an average age of 42.35 ± 10.29 years. Group PNCFN showed the highest stone-free rates (SFRs)(73.7 vs 66.7 vs 80.2, P=.00), best patient satisfaction (89.84 vs 87.23 vs 92.29, P=.00). The length of stay was shorter in group RIRS than other two groups (5.22 vs 5.65 vs 3.72, P=.00). Haemoglobin decrease (> 10 g/L) was higher in group PNL than that in group RIRS and group PNCFN (P=.012). Hospitalization fees (RMB) were Increased in group PNCFN compared with that in group PNL and group RIRS (34563.45 vs 21334.69 vs 33343.16, P=.000). Treatment protocols of PNL decreased from 17.51% to 9.22%, those for RIRS from 5.22% to 17.69%, peaking at 2012, PNCFN from 8.29% to 15.67% showed a rapid growth trend.

Conclusion: The percutaneous nephrolithotomy combined with flexible nephroscopy treatment on renal calculi of 2-4 cm was associated with higher stone-free rates and better patient satisfaction than RIRS and PNL. Comprehensive consideration and conservative use for RIRS treatment of 2-4 cm renal stones.

Laparoscopic Pyelolithotomy for the Management of Large Renal Stones with Intrarenal Pelvis Anatomy

Nasser Simforoosh, Mohammad Hadi Radfar, Reza Valipour, Mehdi Dadpour, Amir Hossein Kashi

Urology Journal, 2019, 5 January 2019
https://doi.org/10.22037/uj.v0i0.5576

Purpose: The role of laparoscopic pyelolithotomy in the management of renal stones is evolving. One of the challenges in LPL for renal stones are patients with intrarenal pelvis. Here we present our experience with laparoscopic pyelolithotomy for the management of renal stones with intrarenal pelvis anatomy.

Materials and Methods: Patients candidate for laparoscopic pyelolithotomy from February 2014 to March 2015 were included. Intrarenal pelvis was defined as > 50% of renal pelvis area contained inside renal parenchyma.  Laparoscopic pyelolithotomy was done by transperitoneal approach. Residual stones were checked by computed tomography and/or intravenous pyelography and ultrasonography 6 weeks after the operation.

Results: 28 patients were included in this study. The mean±SD of patients’ age was 45.8±12.5 years. 19 patients (68%) were male. Stone locations were pelvis, multiple and staghorn in 22, 3, and 3 patients respectively. The mean±SD of operation duration was 160±48 minutes. Residual stones were observed in 3 patients with multiple (n=2) or staghorn (n=1) stones. Urinary leak was observed in 3 patients and was managed conservatively in 2 patients. In one patient ureteral stent was inserted by cystoscopy. No conversion to open surgery or re-operation occurred.

Conclusion: Laparoscopic pyelolithotomy is a feasible operation for patients with renal stones and intrarenal pelvis in centers with adequate experience in laparoscopy. However, the success of LPL decreases in patients with multiple stones and intrarenal pelvis.

Purpose: To determine the efficacy and safety of PCNL in patients with positive urine culture without an any other risk factors prior to surgery, and to define an optimal pre-operative antibiotic regimen for these patients.

Materials and methods: The study included 269 consecutive PCNL cases. These cases were divided into 2 groups according pre-operative urine culture results: sterile (group 1, n=166) and positive (group 2, n=103). Patients with risk factors linked to infection complications were excluded from study. All patients underwent PCNL in the prone position. In group 1, the antibiotic regimen included parenteral injection 30 minutes prior to operation and for 3 days after surgery. Group 2 was given antibiotics 24 hours before PCNL as well as 30 minutes before PCNL and then for 3 days following surgery. On the first day after the operation low dose CT and common blood count were performed on all patients to determine residuals, hematomas, blood loss, and inflammatory markers. 

Results:  Mean age, stone size, failed ESWL, and prior nephrostomy tube insertion were higher in group 2. Although rate of pre-stented patients was equal in groups. No significant differences were observed between group 1 and 2 in regard to operative time (74,3±26,9 vs 70,2±26,5  min, P=.52), length of stay (3,9±1,2 vs 3,8±1,6 days, P=.24), SIRS (6,0% vs 7,8% patients, P=.07), and leukocyte levels exceeding  10*10*9 (77 (46,4%) vs 49 (47,6%) P=.11). Moreover, there was no sepsis or hemotransfusion in either group. Stone-free rates were also similar (78,9% vs 77,7%, P=.35).

Conclusion: 24-hours continuous antibiotic administration before the operation (paying respect to specific resistance bacterial features) can be considered as alternative to 1-week treatment and allow to perform PCNL with sufficient safety in selected patients. Infected urine is not an independent risk factor of post-operative infections complications after PCNL in low risk patients with kidney stones.

Purpose: This study aimed to investigate the efficacy and safety of febuxostat in patients with radiolucent nephrolithiasis.

Materials and Methods: From March 2016 to June 2018, data of 96 patients with radiolucent nephrolithiasis and hyperuricemia who referred to the Third Affiliated Hospital of Sun Yat-sen University were retrospectively analyzed. These patients were divided into allopurinol 300mg/d (control), febuxostat 40mg/d (F40) and 80mg/d (F80) groups respectively. All patients took potassium citrate as a combination treatment and had been followed up for at least 6 months. Before treatment and on the day of the 1st, 3rd and 6th month, complete blood count, serum uric acid (sUA), hepatic and renal function as well as ultrasound were carried out, arthritic and gastrointestinal symptoms were monitored. Computed tomography was measured before treatment and 6 months after medication.

Results: 31, 29 and 30 patients were included in the final analysis of treatment efficacy respectively. Compared with allopurinol group, F40 group showed no difference in urate-lowering effect, while F80 had the best effect across all the visits (P<0.01). At 6th month, 25(83.3%) cases of F80 group achieved sUA<6mg/dL, which was better than allopurinol group (18 cases, 58.1%) and F40 group (17 cases, 58.6%). In the dissolution effect of radiolucent calculi, F80 had the best effect, followed by F40 and then allopurinol (P<0.05). No statistical difference was observed in adverse events among three groups.

Conclusion: Febuxostat significantly decreased sUA, promoted radiolucent stone dissolution and reduced the total stone number, whereas it didn’t increase the adverse events.

Purpose: NTrap® stone entrapment and extraction device (NTrap®) is a device used to extract and remove stones from the urinary tract and to minimize retrograde stone migration during ureterolithotripsy (URS). This study aimed to evaluate the efficacy and safety of NTrap® in URS.

Methods: From Jan 2014 to June 2017, 148 patients underwent URS with the aid of NTrap® (Group A), and 209 patients underwent standard URS without any anti-retropulsion device (Group B). Their demographics, operation time, complications, stone migration rate, and stone-free rate (SFR) were recorded for comparison.  

Results: Compared with group B, Group A had a significantly shorter operative time and lasering time (P=0.003, P=0.000, respectively). There was no significant difference between the 2 groups in overall complications, a decrease in mean hemoglobin, and length of stay (LOS) (P =0.426, P =0.097, P =0.058, respectively). The incidence of stone migration was significantly lower in Group A than Group B (P=0.035). The postoperative auxiliary procedure rate (in patients with stones retropulsion during the operation) was significantly lower in Group A compared to Group B (P=0.024). The SFR was considerably higher in Group A than Group B (P=0.009).

Conclusion: URS, with the aid of NTrap®, is an effective and safe method for treating ureteric stones. It may prevent stones from retropulsion and shorten the operative time. 

Purpose: Percutaneous-nephrolithotomy (PCNL), is the current modality of choice for large renal stones. Delayed post-op bleeding may herald pseudo aneurysm (PA) or arteriovenous fistula (AVF) necessitating costly and inconsistently available angioembolization, or prolonged hospitalization. The goal of this study is to identify criteria that may predict response to conservative therapy, for delayed bleeding from post PCNL intrarenal vascular lesions.


 


Materials and methods: We reviewed all data on patients re-admitted for post PCNL gross hematuria at our high volume center between 2011 and 2016. Perioperative findings, factors related to the stone and management details, were subjected to multifactorial analysis. Logistic regression for multivariable analysis and ROC curves to find thresholds predicting mandatory angioembolization.


 


Results: Of 4403 PCNLs, 83 (1.9%) with delayed bleeding were diagnosed with intrarenal vascular lesions: Arteriovenous fistulas in 54 (AVF, 65%) and pseudoaneurysm in 29 (PA, 35%). Overall 49 (59%) responded to conservative management but 34 (41%) eventually required angioembolization. On multivariable analysis, predictive factors for poor response to conservative treatment were requiring transfusion beyond initial stabilization, pseudoaneurysm, history of open renal surgery, longer interval-to-second-admission, and size of vascular lesion. The proposed POPVESL score (short for Post PNL Vascular Embolization selection) when below 11, correctly predicts success of conservative management with 81.6% sensitivity & 100% specificity.


 


Conclusions: Our findings including the proposed POPVESL score have the potential for clinical application and enhancing practical guidelines on the management of post-PCNL bleeding.

ORIGINAL PAPER (LAPAROSCOPIC AND ROBOTIC UROLOGY)


Robot-assisted Laparoscopic Pyeloplasty in Adults: A Comparison Analysis of Primary versus Redo Pyeloplasty in a Single Center

Najib Isse Dirie, Mahad A Ahmed, Mohamed Abdulkadir Mohamed, Zongbiao Zhang, Shaogang Wang

Urology Journal, 2019, 5 January 2019 , Page 5257
https://doi.org/10.22037/uj.v16i7.5257

Purpose: Approximately 10% of all primary pyeloplasty will require at least one secondary intervention. Our aim was to analyze whether secondary repair will pose additional challenges during robotic pyeloplasty compared with the primary pyeloplasty.


Material and Methods: 114 patients who underwent robot-assisted laparoscopic pyeloplasty (RALP) between February 2015 and August 2018 were retrospectively reviewed. Patients were divided into; primary and secondary repair pyeloplasty. The demographics, intraoperative parameters, postoperative parameters, and success rate of these two groups were collected and compared. Primary RALP data were further stratified into those who previously underwent ipsilateral endourological surgeries (IES) at the obstruction site and those who did not, to evaluate the effect of IES has on the outcome of RALP. Success was defined as symptomatic and radiological relief.


Results: Of the 114 patients, five complicated cases (three horseshoe kidneys, one duplicated system, and one retrocaval ureter) were excluded from the comparison. The remaining 96 primary and 13 secondary repairs were compared. Intraoperative and postoperative parameters showed no significant difference between the two groups. The results of 99 patients (87 vs. 12 in primary vs. secondary, respectively) were available after 27.5 months mean follow-up. The overall success was 92%, 8 patients failed (5 vs. 3 in primary vs. secondary, respectively) and required further surgical interventions.


Conclusion: Though surgically challenging with increased recurrence rates according to the literature we reviewed. However, our data failed to show any significant difference between the primary and redo RALP perhaps due to the smaller size in the redo RALP group.

Purpose: To compare the erectile function and urinary continence of patients after single-port transvesical laparoscopic radical prostatectomy (STLRP) with intrafascial endoscopic extraperitoneal radical prostatectomy (IEERP).


Materials and Methods: Patients treated with STLRP (35) or IEERP (52) were recruited from September 2013 to June 2017. At baseline preoperatively and 2-year follow-up postoperatively, sex and continence assessments were performed by International Index of Erectile Function-6 (IIEF-6) and daily pads, respectively.


Results: The sexual function at 3 months after RP declined obviously. 71.4% (STLRP) and 38.5% (IEERP) patients recovered potency at 6 months postoperatively (P < .01). 82.9% (STLRP) and 59.6% (IEERP) patients recovered potency at 2 years postoperatively (P < .05). 97.1% (STLRP) and 75.0% (IEERP) patients recovered continence (0 pad/day) at 3 months postoperatively (P < .01). Continence achieved 100.0% at 2 years after RP in both groups.


Conclusion: Patients receiving STLRP may obtain better and faster postoperative functional recovery than the ones receiving IEERP. As an exploratory research, STLRP may be another effective treatment for organ-confined prostate cancer.

Symptom Resolution and Recurrence Outcomes after Partial Versus Total Laparoscopic Adrenalectomy: 13 years of Experience with Medium-Long Term Follow up

Nasser Simforoosh, Mohammad Hossein Soltani, Hamidreza Shemshaki, Milad Bonakdar Hashemi, Mehdi Dadpour, Amir H Kashi

Urology Journal, 2019, 5 January 2019 , Page 6338
https://doi.org/10.22037/uj.v16i7.6338

Background: Partial adrenalectomy (PA) is an emerging modality typically performed for the treatment of hereditary and sporadic bilateral tumors, to reduce the risk of adrenal failure. In this study, we evaluated the recurrence and functional outcomes after partial and total adrenalectomy (TA).


Materials and methods: From March 2005 to July 2018, 284 patients with functional tumor or > 5 cm adrenal mass underwent clipless and sutureless laparoscopic partial or total adrenalectomy (PLA and TLA). Patients with a pathological diagnosis of pheochromocytoma, Cushing or Conn’s disease and more than two yea follow up were included in this study.  Pre-operative and operative variables were collected retrospectively and functional outcomes and recurrence were gathered prospectively.


Results: One hundred forty patients (mean age: 43±5.1years) were included in the study. PLA and TLA were performed for pheochromocytoma (total n=78; PLA=12 (15%), TLA=66 (85%)), Cushing syndrome (toal n=17; PLA = 4 (24%), TLA = 13 (76%)), and Conn’s disease (total n=45; PLA=7 (16%), TLA=38 (84%)). In pheochromocytoma patients, improvement of hypertension, palpitation, and headache was not different between patients who underwent PLA versus TLA (all P > 0.05).  Two recurrences were observed in patients with pheochromocytoma and they had undergone TLA. In patients with Cushing disease, central obesity, fascial plethora and hypertension were improved in all patients six months after treatment, muscle weakness was improved one year after surgery, and acne and hyperpigmentation only improved two years after surgery. Length of time for resolution of symptoms was not different in patients who underwent PLA versus TLA. In Conn’s disease hypertension was resolved in all patients and no patient required potassium supplements post-operatively. In follow up no recurrence was observed in patients with a pathological diagnosis of Cushing or Conn’s disease.


Conclusion: In our experience, PLA can provide excellent control of the symptoms parallel with TLA and with no statistically significant difference in recurrence making PLA an attractive option in patients with an adrenal mass.

ORIGINAL PAPER(UROLOGICAL ONCOLOGY)


Purpose: To evaluate the efficacy of caudal regional anesthesia and local anesthesia methods in prostate biopsy applied under transrectal ultrasonography.

Matherials and Methods: This prospective study included a total of 160 patients randomly separated into 4 equal groups as intrarectal local anesthesia (IRLA), periprostatic local anesthesia (PPLA), combined local anesthesia (IRLA+PPLA), and caudal regional anesthesia (CRA). The patients were evaluated using the pain scores on a visual analog scale.

Results: The pain score during anesthesia induction was significantly higher in the CRA group than in the IRLA and IRLA+PPLA groups (p<0.001). The pain score during entry of the probe to the rectum and movement was significantly lower in the CRA group than the IRLA groups (p=0.014). The pain score on penetration of the needle to the prostate and at 30 mins after the biopsy was significantly higher in the IRLA group (p<0.001). At 2 hours after the biopsy, the pain score in the CRA group was significantly lower than IRLA groups (p=0.015).

Conclusion: The PPLA alone can be applied more quickly than CRA, causes less pain during the application, and has similar efficacy in reducing pain during and after the prostate biopsy procedure.

Purpose: This study aimed to explore the security and feasibility of three-dimensional(3D) printing technology assisted laparoscopic cryoablation to treat small renal tumors.

Patients and Methods: 4 patients recruited from April 2016 to August 2017 in our hospital underwent this operation. Three-dimensional reconstruction technology was used to mimic cryoablation treatment before operations in terms of how many needles this process needed and the depth and angle required to insert the needles into the tumor to precisely reserve nephrons. CT scan was used to assess the effect of the treatment after operation in regular follow-up.

Results: All cases were performed this operation successfully and recovered without major complications. The operation time were range from 106 to 118 minutes; the blood lose were range from 50 to 100ml. The follow-up time were between 16-8 months, and the mean time was 13.3 months. Follow-up survey was conducted regularly based on a standard protocol in outpatient. The results showed no abnormal reinforcing signals in cryoablation treated areas.

Conclusion: 3D printing technology assisted laparoscopic cryoablation is a feasible method to treat renal tumors, which maybe a better way to preserve nephrons, especially for those elderly and/or comorbid patients.


Randomized, Double-blind Pilot Study of Nanocurcumin in Bladder Cancer Patients Receiving Induction Chemotherapy

Saleh Sandoughdaran, Abolfazl Razzaghdoust, Ali Tabibi, Abbas Basiri, Nasser Simforoosh, Bahram Mofid

Urology Journal, 2019, 5 January 2019
https://doi.org/10.22037/uj.v0i0.5719

Purpose: To evaluate the feasibility and potential efficacy of nanocurcumin supplementation in patients with localized muscle-invasive bladder cancer (MIBC) undergoing induction chemotherapy.

Materials and methods: In this double-blind, placebo-controlled trial, 26 MIBC patients were randomized to receive either nanocurcumin (180 mg/day) or placebo during the course of chemotherapy. All patients were followed up to four weeks after the end of treatment to assess the complete clinical response to the chemotherapy as primary endpoint. Secondary endpoints were the comparisons of chemotherapy?induced nephrotoxicity, hematologic nadirs, and toxicities between the two groups. Hematologic nadirs and toxicities were assessed during the treatment.

Results: Nanocurcumin was well tolerated. The complete clinical response rates were 30.8 and 50% in the placebo and nanocurcumin groups, respectively. Although nanocurcumin was shown to be superior to placebo with respect to complete clinical response rates as the primary endpoint, there was no significant difference between the groups (p = 0.417). No significant difference was also found between the two groups with regard to grade 3/4 renal and hematologic toxicities as well as hematologic nadirs.

Conclusion: These preliminary data indicate the feasibility of nanocurcumin supplementation as a complementary therapy in MIBC patients and support further larger studies. Moreover, a substantial translational insight to fill the gap between the experiment and clinical practice in the field is provided.

Incidence, Gleason Score and Ethnicity Pattern of Prostate Cancer in the Multi-ethnicity Country of Iran During 2008-2010

Abbas Basiri, Babak Eshrati, Ali Zarehoroki, Shabnam Golshan, Nasser Shakhssalim, Alireza Khoshdel, Amir Hossein Kashi

Urology Journal, 2019, 5 January 2019
https://doi.org/10.22037/uj.v0i0.5618

Purpose: To investigate the geographical incidence, and grade of prostate cancer in Iran during 2008-2010 and evaluate its relationship with ethnicity.

Materials and Methods: Data was extracted from the nationwide Iranian cancer registry system during 2008-2010. Pathologies and grade was extracted from scanned reports of patients’ pathologies by a urologist.

Results: The average 3-year age standardized incidence rate of prostate cancer during the study period was 11.52 per 100000 males. The age standardized incidence rates for Persian, Arab, Turkish and Turkmen, Lor, Kurd and Baluch ethnicities were 13.5, 9.3, 7.9, 7.9, 7.2 and 2.1 per 100000, respectively. Poisson regression analysis revealed a statistically significant difference in incidence of prostate cancer in Baluch ethnicity (P=0.028) and a near significant difference for incidence of prostate cancer in Turk-Turkmen and Kurd ethnicity (P=0.067 and P=0.082) in comparison with Persian ethnicity. The median Gleason score distribution of prostate cancer was not concordant to the age standardized incidence rates. 97% of all pathologies were adenocarcinoma of the prostate followed by malignant carcinoma (1.9%), and transitional cell carcinoma (1.1%).

Conclusion: The incidence of Prostate cancer was different between Baluch and Fars ethnicities in Iran. The lowest ASR of PCa was observed in Baluch ethnicity, however the possibility of underreporting due to less access in Baluch ethnicity cannot be ruled out. The Gleason distribution pattern was not concordant to the incidence distribution of Prostate cancer.

Comparison of the Diagnostic Performance of PI-RADS V1 and PI-RADS V2 for the Detection of Prostate Cancer: A Meta-Analysis Study

Ying He, Ruochen Cong , Jie Zhou, Zhenyu Xu , Jushun Yang, Lin Wang, Jing Xiao , Bosheng He

Urology Journal, 2019, 5 January 2019 , Page 5532
https://doi.org/10.22037/uj.v16i7.5532

Purpose: In order to comprehensively determine the diagnostic accuracy of the Prostate Imaging Reporting and Data System version 1 (PI-RADS V1) and PI-RADS version 2 (PI-RADS V2) in prostate cancer (PCa) diagnosis.


Materials and Methods: The literatures were screened from the databases, including the Pubmed, Embase, Web of science and Cochrane Library up to January 20th, 2020. The meta-analysis was conducted by Meta-DiSc and quality assessment was performed by using the QUADAS. Furthermore, the sensitivity, specificity, likelihood ratio (LR), diagnostic odds ratio (DOR), as well as receiver operating curve (ROC) related to diagnostic accuracy were pooled.


Results: A total of 6 articles containing 814 participants (379 patients) were included in the study. For PI-RADS V1, the combined sensitivity, specificity, PLR, NLR and DOR were 0.82 (95% CI: 0.77-0.85), 0.81 (95% CI: 0.77-0.85), 4.58 (95% CI: 2.55-8.22), 0.24 (95% CI: 0.18-0.34) and 24.00 (95% CI: 10.38-55.51). With regard to PI-RADS V2, the combined sensitivity, specificity, PLR, NLR and DOR were 0.88 (95% CI: 0.84-0.91), 0.81 (95% CI: 0.77-0.84), 4.34 (95% CI: 1.98-9.49), 0.16 (95% CI: 0.08-0.32) and 33.39 (95% CI: 15.05-74.05), respectively. Furthermore, except that the sensitivity of PI-RADS V2 was significantly greater than that of PI-RADS V1 (P=0.027), there was no remarkably difference in other indicators for the diagnosis of PCa between the two versions.


Conclusion: Both PI-RADS V1 and PI-RADS V2 showed good diagnostic performance for PCa diagnosis; moreover, there was no difference in the diagnostic effect between them.

Purpose: To report the 1-year functional outcomes, oncologic outcomes, and postoperative complications in patients who underwent modified robot-assisted radical prostatectomy (RARP) procedures for achieving early recovery of continence and potency postoperatively.


Materials and Methods: This study included 165 patients who underwent RARP. Overall, 98 patients underwent RARP using our modified detrusorrhaphy and intrafascial nerve-sparing techniques (group 1) and 67 underwent standard RARP (group 2). Continence and potency rates were assessed at 1 week, 1, 3, 6, and 12 months after RARP. Oncologic outcomes comprised positive surgical margins (PSMs) and biochemical recurrence (BCR) rate.


Results: The continence rates were 61.2% and 6.0%, 72.5% and 11.9%, 79.6% and 20.9%, 91.8% and 58.2%, and 97.9% and 74.6% at 1 week, 1, 3, 6, and 12 months in group 1 and 2, respectively. The potency rates were 66.3% and 11.9%, 78.6% and 38.8%, 85.7% and 50.8%, 92.9% and 70.2%, and 95.9% and 79.1% at 1 week, 1, 3, 6, and 12 months in group 1 and 2, respectively. Overall postoperative complication rates (< 10%) were similar between the  groups. The PSMs rate was 17.4% and 16.4% in the two groups. The rate of PSMs in the cohort of patients with stage pT2 disease decreased to 13.6% and 12.5% in groups 1 and 2,  respectively. BCR rate was 5.1% and 6.0% in groups 1 and 2, respectively.


Conclusion: The use of detrusorrhaphy and intrafascial nerve-sparing techniques is safe and feasible, with our results demonstrating early return to continence and potency. Further studies should be conducted.

Objectives: The effects of metformin on prostate volume and prostate-specific antigen (PSA) were investigated.


Materials and Methods:: We enrolled 384 newly diagnosed diabetes mellitus (DM) patients and 152 controls all of whom were >50 years into our prospective cross-sectional observational study. The first group contained patients receiving metformin only, the second group were taking a mixture of medications, including metformin plus other oral anti-diabetics, and the third was the control group. Before beginning treatment, body mass indices (BMI) of all cases were obtained. Prostate volumes evaluated using transabdominal ultrasonography at the sixth and twelfth month. Insulin, glycosylated hemoglobin (HbA1C), insulin sensitivity index (ISI), insulin-rich growth factor (IGF-1), PSA, free PSA, and total testosterone levels were measured.


Results: The differences in BMI between the first and third group were statistically significant (p <0.05). There were no statistical differences among the groups in terms of prostate volumes (> 0.05). The differences between the groups for insulin, HbA1C, ISI, IGF-1 (somatomedin), PSA, free PSA and total testosterone levels were insignificant (p >0.05).


Free PSA and total testosterone levels in groups 1 and 2 were not statistically different at the beginning of treatment and the sixth month (p >0.05), but within groups 1 and 2, only PSA levels were different at the start of the study until completion. No differences were seen in the third group.


Conclusion: Metformin appears to cause a decrease in PSA levels. The mechanism and any effects on prostate tissue will be studied in future randomized, prospective studies.

Discrepancy between Needle Biopsy and Radical Prostatectomy Gleason Score among Patients with Prostate Cancer

Amir Reza Abedi, Abbas Basiri, Nasser Shakhssalim, Ghazal Sadri, Mahsa Ahadi, Seyyed Ali Hojjati, Samad Sheykhzadeh, sajjad askarpour, Saleh Ghiasy

Urology Journal, 2019, 5 January 2019 , Page 5985
https://doi.org/10.22037/uj.v16i7.5985

Purpose: Gleason score (GS), as well as other prognostic and diagnostic modalities, can predict the possibility of tumor growth and metastasis during the life of patients with prostate cancer. Based on the prostate biopsy GS, clinicians choose the most appropriate therapy for managing patients. The objective of this cross-sectional study was to determine the discrepancy between needle biopsy and radical prostatectomy GS and to identify its predictive factors among the Iranian population.


Materials and Methods: A total of 1147 patients who underwent radical prostatectomy from 2009 to 2019 were initially enrolled in this study. After consideration of the inclusion and exclusion criteria, 439 patients were finally included. The demographic variables and clinical data including age, PSA level, prostate volume, PSA density, GS derived from ultrasonography-guided core needle biopsy specimen, and GS derived from radical prostatectomy specimen were collected from the medical records of patients with prostate adenocarcinoma and were reviewed by a urology resident. Statistical analysis was done by using the Social Sciences Software version 21.


Results: The average age of patients was 64.5 years (range 48‐84 years), and the average preoperative PSA level was 14.8 ng/mL. On histopathological examination, no changes in GS were observed in 237 (53.9%) patients, whereas GS was upgraded in 144 (32.8%) patients and downgraded in 58 (13.2%) patients at radical prostatectomy. The number of patients who had extracapsular extension, seminal vesicle invasion and positive lymph nodes was significantly higher in the upgraded group compared with the non-upgraded group. 


Conclusion: In this study, there was a steady decrease in GS upgrading with the prostate size extending up to 49.7 g. There was also an association between downgrading and extending prostate size. Due to the greater risk of high-grade disease in men with small prostates, smaller prostate bulks are most probably upgraded after radical prostatectomy. A higher maximum percentage of involvement per core was an independent predictive factor of upgrading from biopsy grade 1 to grade ≥ 2. Our study showed that patients’ age was not predictive of upgrading, which is consistent with other studies. Also, we demonstrated a non-significant relationship between PSA level and upgraded GS. Findings in this study did not demonstrate a significant relationship between PSA level and upgrading.

Pseudocapsule of Small Renal Cell Tumors: CT Imaging Spectrum and Correlated Histopathological Features

Gang Li, Xianqi Lu, Yunsheng Ding, Qiang Luo, Liang Xu, Dongsheng Zhu, Changyi Quan

Urology Journal, 2019, 5 January 2019 , Page 5907
https://doi.org/10.22037/uj.v16i7.5907

Purpose: To systematically analyze histopathologic features of pseudocapsule in small renal cell tumor (diameter≤4cm), assess the integrity of pseudocapsules by Computed Tomography (CT), and provide theoretical basis for the safety of nephron sparing surgery.


Materials and Methods: The pathological data of 116 patients who underwent surgery with clear cell renal cell carcinoma admitted from May 2010 to October 2017 were retrospectively analyzed. All patients underwent a CT scan of the abdomen including an unenhanced and triple-phase (arterial, nephrographic and excretory) post contrast series."All patients underwent a CT scan of the abdomen including an unenhanced and triple-phase (arterial, nephrographic and excretory) post contrast series."All patients underwent a CT scan of the abdomen including an unenhanced and triple-phase (arterial, nephrographic and excretory) post contrast seriesAll patients underwent a CT scan of the abdomen including an unenhanced and three-phase (arterial, nephrographic and excretory) post contrast series. Thorough gross examination and histological sections were used to determine the integrity of the pseudocapsule by two uropathologists. The consistency between pathological findings and CT imaging were evaluated by Kappa consistency test.


Results: The mean diameter of tumor was 3.0cm, range (2.6 ± 0.8) cm. On CT the pseudocapsule can present with one of the three following feathers:1) A regular and distinct halo; 2)lobulated clear margins;3) blurred margins. On histopathology, complete psuedocapsule was found in 85 tumors, incomplete psuedocapsule in 25 and no psuedocapsule was found in 6 tumors; CT scan findings demonstrated a regular halo in 82 tumors, lobulated clear margins in 26 and blurred margins in 8 tumors(Kappa=0.833,P=0.000).


Conclusions: Most small renal cell tumors have an obvious psuedocapsule. Preoperative determination of the psuedocapsule’s integrity is particularly important. CT scan can reliably evaluate the tumor margins and demonstrate the psuedocapsule when present. The imaging results are well correlated with the pathologic findings.

Association of Long Non-Coding RNA MEG3 Polymorphisms and Risk of Prostate Cancer in Chinese Han Population

Bin Xu, Minhao Zhang, Chunhui Liu, Can Wang, Zonghao You, Yali Wang, Ming Chen

Urology Journal, 2019, 5 January 2019 , Page 5585
https://doi.org/10.22037/uj.v16i7.5585

Purpose: To explore the association between MEG3 polymorphisms and the risk of prostate cancer in Chinese Han population.


Materials and Methods: Two MEG3 single-nucleotide polymorphisms (SNPs) (rs11627993 C>T rs7158663 A>G) were genotyped in a case-control study which 165 prostate cancer patients and 200 healthy controls were recruited by a Real-Time Polymerase Chain Reaction (PCR) with the TaqMan assay. The odds ratios (ORs) and 95% confidence intervals (CIs) was used to estimate the strength of association.


Results: No statistically significant differences were found in the allele or genotype distributions of the MEG3 rs11627993 C>T and rs7158663 A>G polymorphisms among cases or healthy control subjects (rs11627993: CC vs CA: 95% CI = 0.54-1.95,ORs = 1.03; CC vs AA: 95% CI = 0.67-2.54,ORs = 1.30 ; CC/CA vs AA: 95% CI = 0.81-1.98,ORs = 1.26 , P = .29 ; C vs A: 95% CI = 0.85-1.57,ORs = 1.16,P = .35; rs7158663: AA vs AG: 95% CI = 0.76-5.08,ORs = 1.97, AA vs GG: 95% CI = 0.57-3.29,ORs = 1.37; AA/AG vs GG : 95% CI = 0.56-1.32,ORs = 0.86,P = .49; A vs G: 95% CI = 0.69-1.39,ORs = 0.98, P = .91) Further stratified analysis detected no significant association, either.


Conclusion: The MEG3 polymorphisms (rs11627993 C>T and rs7158663 A>G) may have no influence on the susceptibility of the prostate cancer. More potentially functionally polymorphisms in MEG3 need to be studied in a larger series.

Purpose: The conventional Trans-Peritoneal Radical Cystectomy (TPRC) harbors numerous postoperative complications, the most prevalent of which are Gastrointestinal (GI) problems. To reduce these morbidities we introduced our own version of extra-peritoneal approach and compared it with the conventional method. 


Materials and Methods: In a cross-sectional observational retrospective design, eligible bladder cancer patients whom underwent Extra-Peritoneal Radical Cystectomy (EPRC) or TPRC in our center, were considered for this study and were compared for early post-operative complications .


Results: Ninety nine patients in TPRC and 81 in EPRC were compared. The two techniques differed in their mean operation time (298.2±37.8 min TPRC vs. 262.8±37.2 min EPRC , P: 0.001). Early GI complications were lower in EPRC groups, including oral intake intolerance ( 21 vs. 8, P: 0.04), ileus (19 vs. 8, P: 0.04),  intestinal obstruction (3 vs. 0, P: 0.04) and anastomosis leak (8 vs. 1, P: 0.01). Urine leak (14 vs.7 , P: 0.02) and wound related complications (19 vs. 6 , P: 0.02) also favored EPRC group.


Conclusion: The extra-peritoneal technique is beneficial in reducing the post operative morbidity, especially the more prevalent GI complications. This approach is functionally safe and allows preservation of the peritoneal integrity.

Needle Tip Culture after Prostate Biopsy: A Tool for early Detection for Antibiotics Selection in Cases of Post-Biopsy Infection

Ian Seng Cheong, Yuh-Shyan Tsai, Chun-Hsiung Kang, Yeong-Chin Jou, Pi-Che Chen, Chang-Te Lin

Urology Journal, 2019, 5 January 2019 , Page 5912
https://doi.org/10.22037/uj.v16i7.5912

Purpose: To investigate biopsy needle tip culture after prostate biopsies for bacteria prediction and antibiotics selection.


Materials and Methods: From May 2017 to April 2019, 121 patients who underwent a prostate biopsy were enrolled. All biopsy needle tips were sent for aerobic and anaerobic culture. Patients were divided into positive and negative culture groups. Perioperative data were recorded and compared between the two groups. The culture time and susceptibility of febrile patients were analyzed. Blood cultures were conducted for all patients who experienced fever after biopsy. The time and results of the needle and blood cultures were recoded for descriptive analysis.


Results: There were 59 (48.8%) positive needle cultures. Other than fever (p = 0.023), there were no statistical significances in clinical data between the two groups. Fever occurred in eight patients, and seven febrile patients had positive needle cultures, six of whom had positive blood cultures. These six needle and blood cultures were consistent with the susceptibility test results. As compared to the waiting time for blood cultures, target antibiotics were administered at an average of 48.0 h earlier based on needle cultures. None of the patients with positive anaerobic cultures developed a fever, while all eight febrile patients had negative anaerobic cultures.


Conclusion: Fevers developed at statistically significant higher rate among those who had positive needle cultures. Needle and blood cultures were consistent with the susceptibility test results. Needle cultures can help us administer target antibiotics earlier to febrile patients without the need to wait for blood cultures.

The Association Between Gelsolin-like Actin-capping Protein (CapG) Overexpression and Bladder Cancer Prognosis

Samira Bahrami, Ali Gheysarzadeh, Mehdi Sotoudeh, Mojgan Bandehpour, Reza khabazian, Hakimeh Zali, Mehdi Hedayati, Abbas Basiri, Bahram kazemi

Urology Journal, 2019, 5 January 2019 , Page 5664
https://doi.org/10.22037/uj.v0i0.5664

Purpose: Muscle-invasive bladder cancer (MIBC) is associated with disease progression and metastasis leading to poor prognosis. Current chemotherapy approaches have not adequately increased patient survival. Therefore, in this study, tissue proteome of patients with MIBC was performed to introduce possible protein candidates for bladder cancer prognosis as well as targeted therapy.

Materials and Methods: The tumoral and normal adjacent bladder tissues were obtained from patients diagnosed with bladder cancer. Two-dimensional gel electrophoresis (2-DE) and liquid chromatography-mass spectrometry (LC-MS/MS) were used to analyze tissue proteome. CAPG protein was further examined using Real-time PCR and western blot analysis.

Results: The 2-DE analysis and LC-MS/MS identified Gelsolin-like Actin-capping (CAPG) protein as differentially expressed protein in tumor tissues of bladder cancer compared with normal adjacent tissues. Western blot analysis showed the CAPG overexpression in tumor tissues compared with normal adjacent tissues in a stage-dependent manner. Correspondingly, Real- time PCR showed a higher mRNA expression in tumoral bladder tissues than normal adjacent ones. CAPG mRNA overexpression had significantly a positive relation with tumor size (p = 0.019), the TNM staging (p = 0.001), and tumor differentiation (grade) (p = 0.006). Patients with lower levels of CAPG had higher recurrence-free survival in comparison with patients with higher level of CAPG (p = .027).

Conclusion: CAPG overexpression was correlated with size, stage, grade, and shorter time to recurrence of tumor. Therefore, CAPG overexpression could be related to poor prognosis of bladder cancer. These results suggest that CAPG may be considered as a prognostic factor and also for targeted therapy in bladder cancer.

Interobserver Variability in Assessment of Renal Mass Biopsies

Łukasz Nyk, Wojciech Malewski, Krystian Kaczmarek, Piotr Kryst, Michał Pyźlak, Aneta Andrychowicz, Tomasz Zabkowski

Urology Journal, 2019, 5 January 2019 , Page 6024
https://doi.org/10.22037/uj.v16i7.6024

Purpose: The main goal of this study was to assess the histopathological efficacy of renal mass biopsy and to check the concordance between pathological results and biopsy of the final specimen, as well as interobserver variability in the assessment of biopsy cores.


Materials and Methods: A hundred sets of core biopsies of postoperative specimens (renal masses) have been performed. Three core biopsies of the intact specimen had been performed once the kidney with the tumor, or the tumor alone were resected. The urologist aimed to obtain two cores from the peripheral sides of the tumor and one core from its center.


The surgical specimen was evaluated by a single pathologist, whereas biopsy samples were referred to three independent pathologists who were blinded to the final results of the renal mass biopsy.


Results: Nondiagnostic biopsy rates ranged from 13% to 22%. Sensitivity and specificity ranged 83-97% and 97-99% by excluding nondiagnostic results. The concordance between assessment of surgical specimen and biopsy in the Fuhrman grading system ranged 36.5-77.0%, respectively. Interobserver agreement between the three pathologists was substantial or moderate, depending on the tumor subtype. The Krippendorff's alpha coefficient, calculated by excluding the nondiagnostic results, was 0.28 (moderate agreement) for the Fuhrman grading system.


Conclusion: The agreement regarding grading of biopsies between three pathologists ranged from moderate to substantial. Therefore, a team of dedicated uropathologists should be engaged in final diagnosis of renal mass biopsy rather than single one before implementing the proper treatment.

Purpose: This work aims to investigate the effects of ΔNp63 gene down-expression on invasion of bladder carcinoma cells in vitro.


Materials and Methods: Bladder carcinoma cell lines UM-UC-3 and 5637 were cultured. The expression plasmids encoding ΔNp63 were constructed and transfected into UM-UC-3 and 5637 cells. The migration and adhesion of cells were detected. The expressions of ΔNp63 and invasion-related zonula occludens protein-1 (ZO-1) in cells were determined by real-time polymerase chain reaction (PCR) and western blot analysis. Confocal microscopy was used to observe the location of ZO-1 in cells.


Results: Results showed that the down-expression of ΔNp63 reduced the migration of UM-UC-3 and 5637 cells, decreased the heterogeneity adhesion, and increased homogeneous adhesion. After transfection with ΔNp63, the ZO-1 expression in cell membrane and cell cytoplasm was inhibited, also the ZO-1 mRNA and protein levels in cells were significantly decreased.


Conclusion: This study indicates thatΔNp63 gene down-expression can reduce the invasion of bladder carcinoma cells in vitro.

Influences of Different Operative Methods on the Recurrence Rate of Non-Muscle-Invasive Bladder Cancer

shoubin Li, Yi Jia, Chunhong Yu, Helong Xiao, Liuxiong Guo , Fuzhen Sun, Dong Wei, Panying Zhang , Jingpo Li, Junjiang Liu

Urology Journal, 2019, 5 January 2019 , Page 5965
https://doi.org/10.22037/uj.v16i7.5965

Purpose: To compare the influence of three operative approaches [transurethral en bloc resection of bladder tumor by pin-shaped electrode (pin-ERBT), transurethral resection of bladder tumor (TURBT) and transurethral holmium laser resection of bladder tumor (HoLRBT)] on the recurrence rate of non-muscle-invasive bladder cancer (NMIBC) at low dimension (i.e. diameter below 3 cm).


Materials and Methods: A retrospective analysis was conducted for a total of 115 patients affected by solitary NMIBC, with a diameter <3 cm, who were submitted to operation between March 2013 to May 2017. The patients were divided according to the operative method applied (pin-ERBT, TURBT and HoLRBT groups, respectively). The 2-year recurrence rate was compared among the three groups, and multivariat Cox hazard model analysis was applied to analyze the influencing factor(s) for postoperative recurrence.


Results: The 2-year recurrence rate was 10.0% in ERBT, 38.5% in TURBT and 40.0% in HoLRBT group, with a significant difference (P =0.014). According to the Cox hazard model analysis, age(HR=1.058, 95% CI: 1.019~1.098,P=0.003), operative method(HR=2.974,6.508, 95% CI: 0.862~10.255,1.657~25.566, P=0.023), smoking(HR=2.399, 95% CI: 1.147~5.017, P=0.020) and pathological grade(HR=2.012,95% CI: 1.279~3.165, P=0.002) were risk factors for postoperative recurrence of bladder cancer.


Conclusion: Pin-ERBT can prominently decrease the postoperative recurrence rate of solitary NMIBC with a diameter <3 cm.

Multiparametric Prostate Magnetic Resonance Imaging before Radical Prostatectomy: Can IT Predict Histopathology?

Mehmet Sahin, Fuat Kizilay, Ezgi Guler, Banu Sarsik, Mustafa Harman, Serdar Kalemci, Adnan Simsir, Ibrahim Cureklibatir

Urology Journal, 2019, 5 January 2019 , Page 6025
https://doi.org/10.22037/uj.v16i7.6025

Purpose: We aimed to investigate the histopathological correlation of the suspected prostate malignancy detected in multiparametric prostate magnetic resonance imaging (mpMRI).


Materials and Methods: The data of 93 patients underwent radical prostatectomy and had preoperative mpMRI were examined. Age and pre-operative Prostate-Specific Antigen value were retrospectively collected from patient files. The pathology specimens were examined again and post-operative ISUP grade group, other pathological findings (seminal vesicle invasion, lymph node involvement and extraprostatic extension), pre-operative mpMRI were re-examined and PIRADS score, extracapsular extension, seminal vesicle invasion, neurovascular bundle invasion, lymph node involvement and ADC values were recorded.


Results: 151 (92,07%) of 164 lesions detected in mpMRI were histopathologically correlated. 80% of patients with seminal vesicle invasion (P < 0.001), 28.8% of patients with extracapsular extension (P < 0.052) and 42.9% of patients with lymph node involvement (P = .001) in mpMRI were histopathologically correlated. A significant relationship was found between PIRADS scores and ISUP grade groups (P < 0.001). There was a negative correlation between ADC values and ISUP grade groups (P < 0.001).


Conclusion: Our study showed that the lesions detected by mpMRI showed a high histopathological correlation.

Responses to targeted therapy among Organs Affected by Metastasis in Patients with Renal Cell Carcinoma are Organ-Specific

Weixing Jiang, Hongzhe Shi, Lianyu Zhang, Jin Zhang, Xingang Bi, Dong Wang, Li Wen, Changling Li, Jianhui Ma, Jianzhong Shou

Urology Journal, 2019, 5 January 2019 , Page 6129
https://doi.org/10.22037/uj.v16i7.6129

Purpose: Previous reports showed that targeted therapy efficacy varied due to different metastatic organs in patients with metastatic renal cell carcinoma (mRCC). This study aimed to further evaluate the response and progression-free time (PFT) of individual metastatic organs.


Materials and Methods: Data from mRCC patients, who were treated with sunitinib between January 2008 to December 2018, were retrospectively reviewed. Individual metastatic organs were assessed separately by The Response Evaluation Criteria in Solid Tumors criteria.


Results: We evaluated response heterogeneity and PFT as characteristics of 281 individual organs affected by mRCC in 213 patients. The objective response rates in these organs were 72.7% in pancreas, 63.7% in spleen, 14.3% in adrenal glands, 13.5% in bone and soft tissue, 11.6% in lymph nodes, 11.6% in lungs, and 9.1% in liver. The median PFT was 15.2 months (95% confidence interval [CI] 2.7–27.7 months) for adrenal glands, 13.2 months (95% CI 3.5–22.9 months) for bone and soft tissue, 9.0 months (95% CI 7.6–10.4 months) for lymph nodes, 8.6 months (95% CI 6.3–10.9 months) for lungs, and 5.2 months (95% CI 2.9–7.5 months) for liver. Median PFT was not reached in pancreas and spleen, but was > 22.8 months and > 20.6 months, respectively.


Conclusion: Our results indicated that organs affected by metastasis may have individual responses to sunitinib treatment. The pancreas and spleen may have the best responses, and liver may have the worst response. Further research is needed to verify these findings.

Purpose: To examine the correlation between prostate specific antigen (PSA) and the risk of Gleason sum upgrading (GSU) from biopsy Gleason sum (bGS) to prostatectomy Gleason sum (pGS).


Materials and Methods: Five electronic databases (Web of Science, Ovid Medline, Ovid Embase, SCOPUS and the Cochrane Library) were searched from inception until March 2020. Studies were included if they focused on the relationship between PSA and GSU analyzed in multivariable analysis. Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were utilized. Quality of included studies was appraised utilizing the Newcastle-Ottawa Quality Assessment Scale (NOS) for case-control studies. The publication bias was evaluated by funnel plot and Egger’s test.


Results: Our search yielded 19 studies with high quality including 42193 patients. GSU was found in 28.2% of patients. Higher PSA level was associated with a significant increased risk of GSU (pooled OR = 1.14, 95% CI: 1.10–1.18; P < .05; I2 = 92%). For the definition of upgrading from bGS ≤ 6 to pGS ≥ 7, the odds of upgrading with higher PSA level as opposed to lower PSA level was 1.12 (95% CI: 1.11–1.14; P < .05; I2 = 13%), while the odds of upgrading with other definitions were 1.11 (95% CI: 1.05–1.18; P < .05; I2 = 89%).


Conclusion: Patients with high level of serum PSA are at high risk of undergoing pathologic upgrading at prostatectomy. Combined with other risk factors, PSA prompts risk reclassification and improve confidence of urologists in management decisions for optimal therapy. Nevertheless, further robust studies are necessitated to confirm these results.

ORIGINAL PAPER (PEDIATRIC UROLOGY)


Safety and Efficiency of Pyeloplasty in The First Six Weeks of Infants' Life

Serdar Moralioglu, Aysenur Cerrah Celayir, Oktav Bosnali, Osman Zeki Pektas

Urology Journal, 2019, 5 January 2019
https://doi.org/10.22037/uj.v0i0.5531

Purpose: The aim of this study was to assess the safety and the efficiency of pyeloplasty in infants with ureteropelvic junction obstruction (UPJO) in the first six weeks of their life.

Materials and Methods: Clinical records of the patients who had surgery during first six weeks of life for UPJO between June 2009 and June 2014 were analysed retrospectively.

Results: In this period, twenty-six dismembered pyeloplasties were performed in twenty-four patients on mean operation age of 27.3 ± 10.2 days (range 8-42 days). On the first postnatal ultrasound all twenty-six renal units had SFU-4 hydronephrosis. Mean preoperative and postoperative anterior-posterior pelvic diameter and parenchymal thickness were 33.1 ± 8.9mm (range 14-49mm), 3.2 ± 1mm (range 1-4,6mm) and 14.7 ± 6.6mm (range 6-27mm) and 7.8 ± 1.9mm (range 3.0-10.4mm), respectively. The differences between preoperative and postoperative parenchymal thickness and anterior-posterior pelvic diameter were statistically significant (P ? 0.0001). Preoperative MAG3 dynamic renal scintigraphy showed obstructive pattern on the diuretic renogram in 26 units. Mean preoperative and postoperative differential renal function on dynamic renal scintigraphy of the effected renal unites was 46 ± 15 and 44 ± 15, respectively. Postoperative drainage was normal on dynamic renal scintigraphy in 25 (96.2%) of the 26 units, redo-pyeloplasty was needed in only one unit (3.8%).

Conclusion: In conclusion, patient selection and timing of surgery are very important in the protection of renal function in newborn with UPJO. In our opinion, if there is indication for surgery, early surgical intervention should not postpone in this period. Surgical treatment of UPJO during first six weeks of life is safe and effective.

Purpose: Vesicoureteral reflux (VUR) is the most common risk factor of urinary tract infection in children. Currently, diagnosis of VUR depends on invasive imaging studies, with high radiologic burden. Therefore, different biomarkers have been introduced for evaluation of these patients.The objective of this study was to identify alteration of urinary interleukins (ILs) excretion in children with primary VUR and renal parenchymal damage, for further clinical application.


Materials and methods: Urinary concentrations of IL-1α, IL-1β, IL-6, and IL-8 were evaluated in 34 children with VUR (cases) and 36 without VUR (control), during 2018-2019. Urinary concentrations of IL-1, IL-1, IL-6 and IL-8 were measured, using polyclonal antibody ELISA kit, and standardized to urine creatinine (Cr).


Patients with infectious or inflammatory disorders, urolithiasis, immune deficiency, acute or chronic  kidney disease, and secondary VUR were excluded from the study.


Results: Mean age of cases (36.00±27.66) had no significant difference with the control (32.86±29.31) group (p=0.44).  The majority of patients had moderate VUR (58.8%), followed by severe (35.3%) and mild (5.9%) grades. Urinary concentration of all ILs/Cr were significantly higher in patients with VUR, compared with those without VUR. There was no significant correlation between urine ILs/Cr with age, gender, serum electrolytes, urine specific gravity, renal ultrasound, laterality or severity of VUR, and DMSA renal scan.


All urine ILs/Cr had acceptable sensitivity and accuracy for workup of children with primary VUR.


Conclusion: Urine IL-1α, IL-1β, IL-6 and IL-8/Cr were sensitive and accurate additionary screening  biomarkers in children with primary VUR.

Purpose: The aim of this study was to evaluate pediatric posterior urethral fibroepithelial polyps, their diagnosis and endoscopic treatments with suprapubic assisted transurethral polyp excision which is described by us.


Materials and Methods: We reviewed the charts of patients (n=6) who underwent suprapubic percutaneous assisted cystoscopic excision for posterior urethral fibroepithelial polyp from 2014 to 2019. Their data were retrospectively reviewed in terms of clinical features, diagnostic methods, endoscopic approaches, and postoperative results.


Results: The 6 patients, the mean age of 3 years (4 months-6 years), with a solitary polyp of posterior urethra diagnosed and removed by suprapubic percutaneous assisted cystoscopic excision in five years. The most common complaint was urinary tract infection (n:3). The urethral polyps were diagnosed by ultrasound and cystoscopy. There was no intraoperative or postoperative complication except for one patient with bleeding from the trocar site. All of the specimens after histopathology examinations showed fibroepithelial polyps and no recurrence was seen.


Conclusion: Posterior urethral polyps may cause obstructive effect in the urinary tract. The treatment should be performed with the least possible invasive method without injuring urethral wall.  We believe that suprapubic percutaneous assisted cystoscopic resection, described by us is an easy, reliable and effective method for treatment procedure of posterior fibroepithelial urethral polyps.

Purpose: To present the medium-term results for the modified Lich-Gregoir (LG) reimplantation technique in the treatment of unilateral primary vesicoureteral reflux (VUR) by comparing patients under and over 12 months of age.


Material and Methods: Data for patients who underwent modified LG surgery between January 2006 and December 2018 were retrospectively reviewed from the hospital data-recording system and patients under the age of 18 years were included in the study. After exclusion criteria, 55 patients in total were included in advanced analysis. The patients were grouped as ≤12 months and >12 months. Demographic characteristics, operative, and postoperative follow-up data were comparatively analyzed.  


Results: The mean age was 10.4 ± 2.8 (6-12) and 41.4 ± 18.5 (13-96) months in the ≤12 months and >12 months groups, respectively. Mean operation time and hospitalization time were not significant between the groups. Mean follow-up times were 39.5 ± 14.1 and 38.4 ± 13.2 months, in the ≤12 months and >12 months groups, respectively. There was no significance in terms of complications between the groups and all of the complications in both groups were in grade 1 category according to the Modified Clavien complication classification. One (6.6%) patient in the ≤12 months group and 3 (7.5%) patients in the >12 months group had late (>30 days) febrile UTI, but none of them had a recurrence of VUR. Febrile infection did not recur during the follow-up period in these patients. While recurrent VUR was not seen in any patient in the ≤12 months group (success: 100%), it was seen in 2 (5%) patients in the >12 months group (success rate: 95%) (p=0.38).


Conclusion: The open LG ureteral reimplantation technique is an effective procedure for the treatment of unilateral primary VUR in children both under 12 months and over 12 months of age with minor morbidity.

Chronic Kidney Disease in Iran: First Report of the National Registry in Children and Adolescences

Neamatollah Ataei, Abbas Madani, Seyed Taher Esfahani, Hasan Otoukesh, Nakysa Hooman, Rozita Hoseini, Mojtaba Fazel, Ali Derakhshan, Alaleh Gheissari, Hadi Sorkhi, Arash Abbasi, Daryoosh Fahimi, Fatemeh Ghane Sharbaf, Fakhrossadat Mortazavi, Behnaz Falakaflaki, Ahmad Ali Nikibakhsh, Simin Sadeghi Bojd, Seyyed Mohammad Taghi Hosseini Tabatabaei, Kambiz Ghasemi, Ali Ahmadzadeh, Parsa Yousefichaijan, Afshin Safaei Asl, Baranak Safaeian, Salman Khazaei, Leila Hejazipour, Abolhassan Seyed Zadeh, Fatemeh Ataei

Urology Journal, 2019, 5 January 2019 , Page 5759
https://doi.org/10.22037/uj.v16i7.5759

Purpose: Knowing the epidemiological aspects of chronic kidney disease (CKD) in children is crucial for early recognition, identification of reversible causes, and prognosis. Here, we report the epidemiological characteristics of childhood CKD in Iran.  


Materials and Methods: This cross-sectional study was conducted during 1991 - 2009.  The data were collected using the information in the Iranian Pediatric Registry of Chronic Kidney Disease (IPRCKD) core dataset.


Results: A total of 1247 children were registered. The mean age of the children at registration was 0.69±4.72 years (range, 0.25 –18 years), 7.79±3.18 years for hemodialysis (HD), 4.24±1.86 years for continuous ambulatory peritoneal dialysis (CAPD), and 3.4±1.95 years for the children who underwent the renal transplantation (RT) (P<.001). The mean year of follow-up was 7.19±4.65 years. The mean annual incidence of CKD 2–5 stages was 3.34 per million age-related population (pmarp). The mean prevalence of CKD 2–5 stages was 21.95 (pmarp). The cumulative 1-, 5-, and 10-year patients' survival rates were 98.3%, 90.7%, and 84.8%, respectively.


The etiology of the CKD included the congenital anomalies of the kidney and urinary tract (CAKUT) (40.01%), glomerulopathy (19.00%), unknown cause (18.28%), and cystic/hereditary/congenital disease (11.14%). 


Conclusions: The incidence and prevalence rate of pediatric CKD in Iran is relatively lower than those reported in Europe and other similar studies. CAKUT was the main cause of the CKD. Appropriate management of CAKUT including early urological intervention is required to preserve the renal function. Herein, the long-term survival rate was higher among the children with CKD than the literature.

Purpose: Concurrent valve ablation and bladder neck incision is suggested as an effective and safe treatment approach in posterior urethral valve children with prominent bladder neck. We evaluated chronic kidney disease risk factors in these children.


Materials and methods: We retrospectively reviewed medical records of children with posterior urethral valve and included those younger than 18 years old who underwent valve ablation and bladder neck incision at our institution. We recorded patient demographics, presenting symptoms, laboratory and radiographic data. Our primary outcome was chronic kidney disease defined as stage 3 chronic kidney disease or higher. Renal outcome risk factors such as preoperative vesicoureteral reflux and serum creatinine, age at diagnosis, adjuvant urinary diversion were analyzed.


Results: A total of 110 patients met our inclusion criteria. Median age at diagnosis was 10.4 months (range 14 days to 12 years). Prenatal diagnosis in 72.7% was the most common presentation. Mean follow-up duration was 3 years and 12(10.9%) patients progressed to chronic kidney disease. Preoperative serum creatinine greater than one mg/dL was the only factor associated with progression to chronic kidney disease.


Conclusion: In our group of children with posterior urethral valve ablation and bladder neck incision, initial creatinine value of greater than one mg/dL is more probably associated with renal impairment while; vesicoureteral reflux, age at diagnosis, presenting symptoms and adjuvant urinary diversion were not significant prognostic factors. Further randomized controlled evaluations are required to analyze the effects of concurrent valve ablation and bladder neck incision on renal outcome.

Objective:  Among the more serious problems in urological interventions among the pediatric age group is the requirement of general anesthesia. The advantages of removing a double-J stent (DJS) without anesthesia in ureteroneocystostomy (UNC) operations among children were investigated in this study.


Patients and Methods: In all, 25 patients who underwent UNC surgery between November 2016 and November 2018 were retrospectively divided into two groups according to the method used for the removal of the DJS. In Group 1, the stent was tied to the urethral catheter by a suture and retrieved postoperatively on the fourth day without anesthesia and cystoscopy. In Group 2, we inserted the stent according to the classical method with no suturing to the catheter and removed it 3 to 4 weeks after the first operation, with cystoscopy under anesthesia.


Results: A total of 16 girls and 9 boys were included in the study. The mean age was 4.3 and 6.3 years in groups 1 and 2, respectively. We did not observe statistically significant difference between the groups in long-term renal function or hydronephrosis regression.


Conclusion: We consider that the removal of a stent placed in pediatric intravesical UNC operations without anesthesia and cystoscopy is less invasive and affords safety and long-term results comparable to the standard method.

ORIGINAL PAPER (KIDNEY TRANSPLANTATION)


Investigating Risk Factors for the Development of BK Virus Infection in Kidney Transplant Recipients in Guilan Province during 2007-2015

Masoud Khosravi, Mahlagha Dadras, Ali Monfared, Siamak Granmaieh, Mohammad Shenagari Rashti, Soheil Soltanipour, Gholamreza Mokhtari

Urology Journal, 2019, 5 January 2019 , Page 5972
https://doi.org/10.22037/uj.v16i7.5972

Purpose: Polyomavirus nephropathy has been recognized as an important cause of silent loss of kidney transplant function in up to 50% of kidney recipients (1). The present study aimed to evaluate the risk factors associated with BK virus infection in kidney transplant recipients.


Materials and Methods: Clinical information, urinary Decoy cells, and blood polymerase chain reaction (PCR) tests were collected for polyomavirus infection in 223 kidney transplant recipients undergoing surgery at Razi hospital at Guilan University of Medical Sciences between 2007 and 2015. Kidney biopsies were performed in patients with BKPyV- DNAemia more than 10,000 Copies/ml or increased plasma creatinine.


Results: Among 223 patients, 116 (52%) were male. The mean age of participants was 49.57±13.48 years. Out of 223 participants, 41 (18.4%) had Decoy cells in their urine, and 182 (81.6%) did not, 15 of whom (6.7%) had viral genome in their blood. Only did 3 patients out of 10 have BK Virus nephropathy in their kidney biopsy. Among risk factors, it was found that post-transplant duration (P< 0.001) and the use of anti-thymocyte globulin (P= 0.001) were the most significant risk factors for finding decoy cells in patients’ urine.


Conclusions: Post-transplant time, particularly the first 6 months, was found as the most important risk factor for the reactivation of polyomavirus infection in our patients because of strong immunosuppression and use of anti-thymocyte globulin (for prophylaxis or rejection treatment). It is concluded that kidney transplant recipients should be monitored in episodically after transplantation.

ORIGINAL PAPER (FEMALE UROLOGY)


Purpose: Pelvic organ prolapse is a common condition  as a consequence of the pelvic floor support weakness. This study evaluated the clinical results of treating the high stage prolapse of the anterior vaginal wall using a trans-obturator approach and the native vaginal wall tissue.


Methods: This was a prospective analysis of 94 patients with anterior vaginal wall prolapse stage ≥ Ⅲ. They underwent surgery with the trans-obturator approach using the native vaginal wall tissue. The objective primary outcome was evaluated according to the pelvic organ prolapse staging system (POP-Q). The subjective primary outcome was evaluated with pelvic floor distress inventory (PFDI-20) and pelvic floor impact questionnaire (PFIQ-7) questionnaires. The secondary outcomes were post-surgery complications.


Results: Totally, 85 of 94 patients were followed up for a mean of 38.2 ± 4 months. The objective anatomical success rate was 90.58%. PDFI-20 and PFIQ-7 scores had improved (P= 0.001). The complications were minor (G1) according to the Clavien-Dindo classification (8.2 %). complained of de novo frequency and urgency which was resolved two months after the surgery. At one year follow up 3 out of 8 patients with clinical SUI underwent transvaginal repair with the Poly propylene mini sling mesh.


Conclusion: The midterm results of the surgical repair of the high stage anterior vaginal wall prolapse are promising with a new surgical technique by trans-obturator approach and native vaginal wall as the supportive layer

Association between Hyposensitivity of C-fiber Afferents at The Proximal Urethra and Storage/voiding Dysfunction in Female Patients with Detrusor Overactivity

Osamu Ichiyanagi, Ken-ichi Nishimoto, Akira Nagaoka, Sei Naito, Mayu Yagi, Masaki Ushijima, Tomoyuki Kato, Norihiko Tsuchiya

Urology Journal, 2019, 5 January 2019 , Page 5515-5515
https://doi.org/10.22037/uj.v16i7.5515

Purpose: We examined the associations between urethral sensation and storage/voiding function in female patients with detrusor overactivity (DO) by measuring urethral current perception threshold (CPT).

Materials and Methods: We retrospectively investigated the medical records of 27 consecutive patients with lower urinary tract symptoms who underwent cystometry, uroflowmetry (UFM), and urethral CPT tests from 2000 to 2015. Patients were classified into 2 groups: with/without DO. Seven DO-negative cases were selected as normal controls on cystometrogram (CMG) matching the inclusion criteria: bladder compliance ?12.5 mL/cmH2O, volume <275 mL at first sensation, and no comorbidities possibly influencing micturition. Finally, 17 patients were included. Urethral CPT was evaluated with intraurethral square-wave impulses at 3 Hz to stimulate C-fibers. Urethral loss coefficient (LC), reflecting urethral resistance during voiding, was calculated by curve-fitting a mathematical model to a UFM waveform.

Results: Urge incontinence (UI) was observed in 7 DO-positive patients, but not in those with normal CMG. Urethral CPT and LC were significantly higher in patients with DO than in those with normal CMG. Median urethral CPT significantly increased in patients with both DO and UI than in those without these symptoms (p<0.005). CPT values were correlated with the volume at first sensation (?=0.53, p<0.05) and LC (?=0.59, p<0.05). LC was not calculated in 3 cases due to poor curve-fitting.

Conclusions: In females, urethral C-fiber afferents may become hyposensitive as the detrusor becomes overactive with UI in the storage phase. During voiding, C-fiber hyposensitivity may relate to increased functional resistance of the urethra to urine outflow.

Assessing the Reliability and Validity of the Persian Version of the Chronic Pelvic Pain Questionnaire in Women

Mahboubeh Mirzaei, Azar Daneshpajooh, Mohammadali Bagherinasabsarab, Fatemeh Bahreini, Fatemeh Yazdanpanah

Urology Journal, 2019, 5 January 2019 , Page 6212
https://doi.org/10.22037/uj.v16i7.6212

Purpose: There is a need for developing a standard and approved tool to assess chronic pelvic pain (CPP) in Iranian women. The aim of this study was to investigate the reliability and validity of the Persian version of the pelvic pain and urinary/frequency (PUF) questionnaire in Iranian women with CPP.


Materials and Methods: This cross-sectional study was performed on 50 females with CPP referred to the urology clinic of Kerman University of Medical Sciences from 2018 to 2019. Initially, the PUF questionnaire was translated into Persian and then back translated into English. The face validity of the tool was evaluated by being tested on 50 patients who had different literacy levels to ensure its understandability and acceptability by patients. The construct validity was evaluated through both exploratory and confirmatory factor analyses. The internal consistency was also analyzed by determining Cronbach's alpha coefficient and test-retest method.


Results: The Persian version of the questionnaire was compatible with the original English version. The Kisser sampling adequacy index was calculated on the data before extracting the factors indicating good factor accessibility of the questionnaire statements. The construct validity of the questionnaire was confirmed using exploratory and confirmatory factor analyses. The internal consistency parameters were also acceptable. Cronbach's alpha coefficient of the whole questionnaire, as well as the coefficients of the "signs/symptoms" and "unpleasant feelings" domains were 77%, 74%, and 78%, respectively.


Conclusion: The developed Persian version of the PUF questionnaire retrieved a good validity and reliability.

ORIGINAL PAPER (ANDROLOGY)


Purpose: In this study the role of nicotine (NCT) administration on the intensity of rat testicular tissue alterations induced by quinine (QU) was evaluated. 


Materials and Methods: Forty adult Wistar rats were divided into four groups. Control (CON), NCT administrated (4 mg/kg) (NCT), QU treated (25 mg/kg for 7 days) (QU), and nicotine with quinine received (NCT+QU). After 28 days, serum testosterone and malondialdehyde (MDA) levels were measured. Testes and epididymides samples were prepared for determining tissue MDA levels, histomorphometry, microscopic indices of spermatogenesis, immunohistochemistry of p53 and sperm analysis.


Results: Testosterone levels were decreased significantly (P=.0004) in treated groups compared to CON group. Serum MDA levels were increased significantly (P=.0004) in NCT and QU groups compared to CON group. Tissue MDA levels were increased significantly (P=.0012) in NCT+QU group in comparison to CON group. These parameters were changed significantly in NCT+QU group compared to QU group. Seminiferous tubules diameter was decreased significantly (P < .0001) in treated groups compared to CON group and in NCT+QU group compared to QU group. The height of germinal epithelium was decreased significantly (P=.0001) in NCT and NCT+QU groups compared to CON and QU groups. The number of Sertoli cells, spermatocytes and spermatids was decreased significantly in treated groups compared to CON group. The number of spermatogonia was decreased significantly (P=.0017) in NCT and NCT+QU groups compared to CON group. The number of Sertoli cells, spermatogonia and spermatocytes was decreased significantly in NCT+QU group compared to QU group. All indices of spermatogenesis were decreased in treated groups compared to CON group. The lowest mean of these indices was observed in NCT+QU group. The sperm viability was decreased significantly (P < .0001) in treated groups compared to CON group. Sperm count and motility were decreased significantly in NCT and NCT+QU groups compared to CON group. All experimental groups showed the over-expression of p53 compared to CON group.


Conclusion: The administration of nicotine could be involved in the exacerbation of testicular tissue alterations related to quinine therapy.

Correlation between Protamine-2 and miRNA-122 in Sperm from Heroin-Addicted Men: a Case-Control Study

Zohreh Nazmara, Mohammad Najafi, Mansoureh Movahedin, Zahra Zandiyeh, Peymaneh Shirinbayan, Hamid reza Asgari, Mohsen Roshanpajouh, Chad Maki, Zahra Bashiri, Morteza Koruji

Urology Journal, 2019, 5 January 2019 , Page 5747
https://doi.org/10.22037/uj.v16i7.5747

Purpose: Recreational use of illicit drugs is one of the main factors affecting male fertility. However, the mechanisms of heroin smoke-associated damage to mature spermatozoa are still completely unknown. The aim of this study was to concomitantly examine the levels of protamine-2 gene and protein concentrations, the amount of miRNA-122 in seminal plasma and semen analysis findings in heroin-addicted men.


Materials and Methods: In a case control study, twenty-four fertile men that lacked any recreational drug abuse were considered as the healthy group, and 24 addicted men who used only heroin for at least four months were selected as the addicted group. Semen samples were gathered by masturbation after 2 - 5 days of sexual abstinence. Following the preparation of a semen analysis by computer-assisted sperm analysis according to WHO (2010), the level of protamine-2 gene expression in sperm and miRNA-122 in seminal plasma was measured using real-time sqPCR. Also, protamine-2 protein concentrations were quantified by nuclear protein extraction, SDS-Page and western blotting.


Results: Among the studied variables, body mass index (27.75±0.88 vs. 22.30±0.36, p=0.001), seminal pH (7.79±0.06 vs. 7.58±0.06, p=0.003), white blood cell count in semen (1.69±0.41 vs. 8.61±1.73, p=0.001), motility (65.51±2.57 vs. 41.96±3.58, p=0.001) and survival rate (87.41±1.00 vs. 71.50±4.59, p=0.002) of sperm cells was significantly different between the healthy and addicted groups. In addition, the levels of protamine-2 gene and protein expression in the addicted group (0.05±0.02 and 0.10±0.02, respectively) were significantly lower than the healthy group (3.59±0.94 and 0.27±0.06, respectively) (p=0.002 and p=0.017, respectively). Seminal miRNA-122 levels in addicted men (3.51±0.73) were statistically higher than in healthy men (1.52±0.54) (p=0.034). However, there were some significant relationship between the studied parameters and addiction (p<0.05).


Conclusion: This is one study on human infertility that evaluates the effects of heroin on protamine deficiency and seminal small RNAs expression levels. Heroin abuse may lead to male infertility by causing leukocytospermia, asthenozoospermia, protamine deficiency, and seminal plasma miRNA profile alteration.

ORIGINAL PAPER (RECONSTRUCTIVE SURGERY)


Major Complications after Male Anti-IncontinenceProcedures: Predisposing Factors, Management and Prevention

Miklos Romics, Gergely Bánfi, Attila Keszthelyi, Hans Christoph Klingler, Tibor Szarvas, Marcell Szász, Péter Nyirády, Attila Majoros

Urology Journal, 2019, 5 January 2019
https://doi.org/10.22037/uj.v0i0.5712

PURPOSE: Significant post-prostatectomy incontinence (PPI) is a crippling condition and managed best through sling or artificial urinary sphincter (AUS) implantation. These procedures are often associated with complications requiring surgical intervention. The aim of our retrospective study was to evaluate the occurrence of major complications and identify risk factors.      
MATERIALS AND METHODS: Between 2010 and 2018 ninety-one patients have been implanted with sling (22; 24.2%) or AUS (69; 75.8%) in our department.  The cases where surgical revision was needed were examined regarding the etiology (mechanical failure (MF), urethral erosion (UE), urethral atrophy (UA), surgical site infection (SSI), combined reasons (COMB) and analyzed, using 16 possible perioperative risk factors.

RESULTS: Surgical intervention was carried out by 19 / 91 (20.9%) patients. (In 16 / 69 cases after AUS (23.1%), 3 / 13 after slings (23%)). The indication was in 6 (31.6%) cases MF, in 3 (15.8 %) COMB, in 4 (21.1%) UE, in 5 (26.3 %) SSI, in 1 (5.2%) UA. The type of reoperation was either explantation (12 / 19), system replacement (6 / 19), or cuff replacement (1 /19). Regarding the surgical intervention requiring complications only preoperative bacteriuria (P = .006) and postoperative surgical site oedema (P = .002) proved to be independent predictive factors. 
CONCLUSION: Preoperative bacteriuria and surgical site oedema seemed to be good predictors for obligate surgical revision. Patients with AUS were more prone to have major complications. In most cases it was mechanical failure, infection or erosion. By reducing the frequency of these risk factors we might be able to decrease the amount of complications.

 

Efficacy Of Preoperative Gabapentin on Pain Severity after Posterior Urethral Surgery:A Randomized, Double-Blind, Placebo-Controlled Study

saleh ghiasy, Ali Tayebi-azar, Amin Alinezhad, Morteza Fallah-karkan, Hojat Salimi, Seyyed Ali Hojjati, Jalil Hosseini

Urology Journal, 2019, 5 January 2019
https://doi.org/10.22037/uj.v0i0.5598

Purpose: Prevention and treatment of urethroplastic postoperative pain is a major challenge in post-surgery care. gabapentin can treat postoperative pain by preventing excessive sensitivity to the central nervous system. we have tried to compare the effect of gabapentin with the placebo on reducing the pain associated with posterior urethroplasty in patients.


Material and Method: This prospective, randomized, double-blind study was scheduled in Shohad e Tajrish hospital where a single dose of Gabapentin was compared with placebo given to patients preoperatively. In this study,100 patients with posterior urethral stricture were included for surgery with 50 patients in each arms .All patients underwent posterior urethroplasty. After surgery, the pain level is assessed and evaluated by the visual analog scale in two hours,four hours, six hours,eight hours, twelve hours, and 24 hours after the operation.


Result: In this study, there was a significant difference in the pain level that was evaluated by the visual analog scale in two hours, four hours, six hours, eight hours, twelve hours, and 24 hours after the surgery (p.value <0.001). We also found a significant decrease in morphine consumption in the gabapentin group vs the placebo group. (p.value <0.001)


The post-surgery assessments showed significant lower adverse effects such as vomiting, nausea, drowsiness, and pruritus in the gabapentin group vs. placebo group.


Conclusion: The consequences of this study revealed that gabapentin effective in controlling posterior urethroplasty postoperative pain, consumption of opioid, nausea, vomiting, drowsiness, and pruritus compared with the placebo group.

IS there a Difference in Platelet-Rich Plasma Application Method and Frequency to Protect Against Urethra Stricture?

Arif Aydin, Mehmet Giray Sonmez, Pembe Oltulu, Rahim Kocabas, Leyla Ozturk Sonmez, Hakan Hakki Taskapu, Mehmet Balasar

Urology Journal, 2019, 5 January 2019 , Page 6100
https://doi.org/10.22037/uj.v16i7.6100

Material-Method:  Fifty rats were randomized into 5 groups, with 10 rats in each group. The urethras of all rats were traumatized with a pediatric urethrotome knife at 6 and 12 o’clock, except in the sham group. Group 1 was the sham group and had only urethral catheterization daily for 15 days, Group 2 was given 0.9% saline (physiologic saline [(UI+PS]) once a day after urethral injury (UI+ PS), Group 3 was injected with PRP submucosally after urethral injury, Group 4 was given PRP once a day as intraurethral instillation using a 22 Ga catheter sheath with urethral injury, and Group 5 was given PRP twice a day as intraurethral instillation using a 22 Ga catheter sheath with urethral injury.


Each administration of PRP was administered as 300 million platelets/150 microliters. On day 15, the penises of the rats were degloved to perform penectomy. Histopathologic evaluation was made for spongiofibrosis, inflammation, and congestion in vascular structures.


Results: When the sham group, UI+PS, UI+PRPx1, UI+PRPx2 and UI+PRPs groups are compared in total, there were significant differences identified for parameters other than edema. When the UI+PS, UI+PRPx1, UI+PRPx2 and UI+PRPs groups are compared, the UI+PS group was observed to have significantly more inflammation (mucosal inf. 2.42 ±0.53) and spongiofibrosis (2.42 ±0.53). All the PRP groups were identified to have significantly less mucosal inflammation (UI+PRPs 1±0, UI+PRPx1; 1.4±0.51, PRPx2; 1.33±0.5) and spongiofibrosis (UI+PRPs; 1.57±0.53, PRPx1; 1.2±0.42, PRPx2; 1.55±0.52). The group with lowest spongiofibrosis was seen to be the PRPx1 group.


Conclusion: This study showed that PRP significantly reduced mucosal inflammation and spongiofibrosis, independent of administration route, when applied to the urethra after urethral trauma

CASE REPORT


This report is about the effectiveness of sacral neuromodulation in a 32-year-old woman with a history of augmented cystoplasty who required clean intermittent catheterization. She had referred to our center with a medical history of bilateral vesicoureteral reflux because of neuropathic lower urinary tract dysfunction. We successfully did a sacral neuromodulation on her which lead to a promising result.

Here, we report a case of a 73-year-old man with a history of hepatocellular carcinoma. Follow-up abdominal computed tomography showed a 1.7-cm-sized hyperenhancing mass in the right paracolic gutter, suspicious of metastasis. Histologic and immunohistochemical study of the resected specimen revealed extragenitourinary ectopic prostatic tissue. Since ectopic prostatic tissue is very rare, preoperative diagnosis is difficult but important for avoiding unnecessary treatment or patient anxiety. Here, we present a rare case of ectopic prostatic tissue in the right paracolic gutter.

Background: This study presents initial experience in endoscopic meatal dilatation of obstructive ureterocele in adult patients.

Surgical technique: During cystourethroscopy, we tried to find the orifice of ureterocele, passed a guide wire and introduce an 8 Fr ureteroscope in to the ureterocele orifice, going up to the renal pelvis as under vision dilatation of ureterocele meatus. Two Double-J stent were inserted and remained for six weeks to keep the meatus dilated. Adverse effect of endoscopic management was decreased due to minimal anatomic changes.

Results: Patients’ symptoms were relieved and no evidence of new onset vesico-ureteral reflux and obstruction were seen after up to one-year follow-up.

Conclusion: Endoscopic meatal dilatation of stenotic ureterocele in adult patients is safe and effective thus, try to find the orifice of ureterocele is suggested.

Persistent müllerian duct syndrome (PMDS) in the majority of cases is discovered during surgery for inguinal hernia or cryptorchidism. A transverse testicular ectopia (TTE) with cryptorchidism may be very rarely associated to PMDS. Assuming that müllerian remnants have a very low malignant degeneration potential if compared to the malignancy risk of an undescended and not relocated testis, we describe a simplified surgical technique of orchiopexy that avoids an extensive anatomical dissection, in this way minimizing the risk of losing the deferential blood supply to the testis.

Appendiceal conduit: A novel technique to be applied after radical cystectomy: A case study report

emadoddin Moudi, Seyyed Hosein Ghasemi Shektaie, Ghasem Rostami

Urology Journal, 2019, 5 January 2019 , Page 5513-5519
https://doi.org/10.22037/uj.v16i7.5513

This paper introduces a novel technique, known as appendiceal conduit. It could be used as an alternative for ileal conduit and cutaneous ureterostomy, ultimately applied after radical cystectomy. The six-month follow-up indicated that the patient had appendix-stoma as nipple without any stenosis, nor did he have any hydronephrosis, as confirmed by abdominal sonography.

Mesothelioma in Situ of the Spermatic Cord Arising from a Patent Processus Vaginalis: A Case Report

Yasuyuki Kobayashi, Yumiko Yasuhara, Hiroki Arai, Masahito Honda, Masataka Hiramatsu, Sho Goya

Urology Journal, 2019, 5 January 2019 , Page 5421
https://doi.org/10.22037/uj.v16i7.5421

Mesothelioma is an aggressive tumor originating from mesothelial cells. Mesothelioma of the spermatic cord is a very rare disease, and the most common presentation of this disease is that of aggressive mesothelioma with no description of mesothelioma in situ. We report an extremely rare case of mesothelioma in situ of the spermatic cord arising from a patent processus vaginalis. To our best knowledge, this is the first report of this finding. The identification of a patent processus vaginalis and investigation of single-layered atypical mesothelial cells led to the final diagnosis.

BRIEF COMMUNICATION


Urinary Human Kidney Injury Molecule-1 (hKIM-1) Is Not Increased in Patients with Renal Cell Carcinoma

Lukasz Bialek, Slawomir Poletajew, Michal Niemczyk, Katarzyna Czerwinska, Mateusz Nowak, Anna Sadowska, Tomasz Borkowski, Piotr Radziszewski, Jakub Dobruch, Piotr Kryst

Urology Journal, 2019, 5 January 2019 , Page 6077
https://doi.org/10.22037/uj.v16i7.6077

Purpose: Human Kidney Injury Molecule-1 (hKIM-1) was proposed as urinary biomarker of renal cell carcinoma (RCC). The aim of the study was to validate urinary hKIM-1 as a biomarker of RCC.


Material and methods: Forty-six participants were enrolled into the study, including 30 patients with clear-cell or papillary RCC and 16 matched patients in the comparison group. Preoperative urinary hKIM-1 levels were measured using commercially available ELISA kit and normalized to urinary creatinine levels.


Results: The concentrations of urinary hKIM-1 normalized to urinary creatinine in patients with RCC and comparison group did not differ significantly (1.35 vs. 1.32 ng/mg creatinine, p=.25). There was also no difference in urinary hKIM-1 concentration regarding stage or grade of renal cancer. Additional analysis of patients without chronic kidney disease (defined as eGFR ≥60mL/min/1.73m²) also did not reveal significant difference in urinary hKIM-1 concentrations between the groups (1.54 vs. 1.37; p=.47).


Conclusion: Results of our study do not confirm recent suggestions that urinary hKIM-1 may be a biomarker of RCC.

LETTER


Current Trends in Hypospadias Repair. Where are we Standing?

Parisa Saeedi Sharifabad, Vahid Poudineh, Mehran Hiradfar, Ahmad Mohammadipour, Reza Shojaeian

Urology Journal, 2019, 5 January 2019 , Page 6081
https://doi.org/10.22037/uj.v16i7.6081

Purpose: Although several surgical approaches are suggested for hypospadias reconstruction but there is not a consensus on the best surgical method of hypospadias repair. This is a survey about technique of choice in treatment of various types of hypospadias.


Method and materials: We asked about preferred method of treatment for three different hypospadias scenarios. 170 surgeons completed the questionnaire and enrolled in this study.


Results: 51 general urologists, 61 pediatric surgeon, 16 plastic surgeons and 42 pediatric urologists joined the survey. Preferred method of surgery for a distal hypospadias was TIP in 53.5%. TIP was offered by 39.4% , Two stage Urethroplasty in 24.7% and Island On lay Flap in 21.2% to repair a proximal hypospadias without cordee. Two-stage Bracka or Durham Smith were common surgical options in proximal hypospadias surgery with cordee.


Conclusion: A growing trend was detected on TIP repair of distal and proximal hypospadias without cordee.

UNCLASSIFIED


Purpose: This study was conducted to determine the effects of tamsulosin and diclofenac sodium use on patients' pain perception after ureteral stents removal.

Materials and Methods: This study was a randomized control trial with double-blinded design. Eighty patients who underwent ureteral stent removal surgery at Kardinah Hospital, Tegal during January to March 2017 were divided into four groups. The experimental group was administered by analgesic for two days, (A) placebo tid, or (B) diclofenac sodium 50 mg bid, or (C) tamsulosin 0.2 mg sid, or (D) combination of tamsulosin and diclofenac sodium. Analgesic effects were assessed with the Visual Analog Scale (VAS). Relationships among variables were assessed using one-way ANOVA and post hoc tests.

Results: The surgical procedure for ureteral stent removal consisted of 48 (60%) male and 32 (40%) female. The average age of group A, B, C, and D were 51.0, 51.9, 47.6, and 47.3 years, and the average stent dwell times was 6.3 weeks. VAS values of the entire experimental group were lower than the control group on the first day until the second day after the stent removal procedure (p <0.05). In the experimental group, there was no difference between group B and C (p> 0.05). Group D showed better analgesic effects than group B and C (p <0.05). There were no severe side effects found in whole cases.

Conclusion: The result shows that combination therapy of diclofenac sodium and tamsulosin is better in reducing the pain after ureteral stent removal compared to the admission of a single placebo, tamsulosin, and diclofenac sodium therapy.

 

Purpose: To delineate the expansion of the renal parenchyma using volumetric CT imaging before and after the laparoscopic cyst decortication procedure and to determine the possible associations between parenchymal expansion and laboratory parameters and cyst volume.

Materials and Methods: Thirty-five patients that underwent laparoscopic cyst decortication were included in this prospective study. Abdominal contrast-enhanced CT was performed in all patients in the preoperative and postoperative period. Semi-automatic volume quantification was undertaken offline, and renal parenchymal volumes before and after cyst decortication, as well as serum creatinine and estimated glomerular filtration rate (eGFR) were compared.

Results: The changes in serum creatinine and eGFR in the postoperative period were non-significant. The mean postoperative renal parenchymal volumes were higher compared to the preoperative measurements for both observations (P = .014 and .034 for the first and second measurements, respectively). There was no correlation between the volumetric change and the cyst volume (r = -0.18, P = .560).

Conclusion: In patients undergoing laparoscopic cyst decortication, post-operative parenchymal expansion can be detected using volumetric CT imaging to confirm the immediate benefits of the procedure.

Validation of the Persian Version of the National Institute of Health Chronic Prostatitis Symptom Index

Farzad Allameh, Mohammad Mersad Mansouri Tehrani, Mohammad Ali Tasharrofi, Mohammad Ali Ganji Jameshouran

Urology Journal, 2019, 5 January 2019
https://doi.org/10.22037/uj.v0i0.5444

Objectives: To compose a comprehensible and fluent Persian translation of the National Institute of Health Chronic Prostatitis Symptom Index (NIH-CPSI), and to determine its linguistic validity in a Persian sample population.

Methods: The standard double-back translation method, provided by the previous studies were utilized by three professional linguists to translate the English version of the NIH-CPSI to Persian, and a group of 10 urologists further reviewed and translated questionnaire. The questionnaire was then presented to the sample study, comprised of 60 men with CP/CPPS and 60 controls with adverse urological history, and the collected data was analyzed through IBM-SPSS software to test its validity, evaluative, and discriminatory power, psychometric qualities and internal consistency.

Results: A total of 80 subjects (42 CP/CPPS patients and 38 healthy controls) were considered eligible for this study. The total Persian NIH-CPSI scores and each subdomain showed significant difference (P < 0.001) between the two study groups, indicating a satisfactory discriminant validity for the index. Psychometric analysis established the index to benefit from a high internal consistency. The translation was also considered by both the subjects and the physicians to be easily comprehensible.

Conclusion: The Persian NIH-CPSI is a reliable and valid instrument for evaluating CP/CPPS symptoms in general population, while also benefitting from high discriminatory power, and can be utilized with ease in both clinical practice and laboratory studies.

Purpose: To describe and report a series of renal and adrenal anastomosing hemangioma (AH) and to investigate its distinctive clinicopathologic features and review its clinical data available in the literature.

Materials and Methods: Clinical data of 10 AHs were retrospectively studied. Imaging and histologic features were re-evaluated and summarized. Immunostaining markers were performed include CD31, CD34, ERG, Fil-1, D2-40, AE1/AE3, SMA, CD10, HHV8, S100, Ki-67. A follow-up of all cases was performed. Other AHs published in PubMed and Web of Science were reviewed.

Results: All of 10 AHs were found incidentally, containing 5 female and 5 male patients (median, 48.5 years; mean, 51.7 years) and involved unilateral kidney (n=7) and adrenal glands (n=3) respectively. All lesions were well-defined in Imaging and histologic examination. AHs were morphologically characterized by prominent anastomosing vascular channels without evidence of infiltration to surrounding normal tissues and significant cellular atypia. CD31, CD34, ERG were positive and Ki-67 showed typically low (?3%). All Patients underwent a mass resection and none of them had evidence of recurrence. Together with other cases published, the AHs showed distinctive clinicopathologic features with an excellent prognosis.

Conclusion: Renal or adrenal AH is a very rare vascular tumor. They have distinctive histologic features and a favorable prognosis. Its frequently mimicking well-differentiated angiosarcomas easily result in unnecessary overtreatment in clinical practice. To be acquainted with this entity serves to avoid misdiagnosis.

Changes in Apoptosis-related Proteins in the Urothelium of Rat Bladder Following Partial Bladder Outlet Obstruction and Subsequent Relief

Jong Mok Park, Ji Yong Lee, Yong gil Na, Ki Hak Song, Jae sung Lim, Seung Woo Yang, Seung-Hwan Lee, Gun-Hwa Kim, Ju Hyun Shin

Urology Journal, 2019, 5 January 2019
https://doi.org/10.22037/uj.v0i0.5799

Purpose: Partial bladder outlet obstruction (PBOO) induces sustained bladder over-distension, leading to ischemia/reperfusion (I/R)-related oxidative damage of the urothelium via apoptosis. The present study aimed to investigate the sequential course of apoptosis in the urothelium of rat bladder and identify the changes in apoptosis-related proteins during PBOO and subsequent relief. 

Materials and Methods: The study was conducted using 60 female Sprague-Dawley rats divided into three groups: sham-operated, PBOO only, and PBOO plus subsequent relief. PBOO was induced for 2 weeks, and then the obstruction was relieved by removal of the ligature. The urothelium was assessed by a histological analysis, and expression levels of apoptosis-related proteins were detected by quantitative PCR and immunoblotting.

Results: Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL)-positive cells were significantly increased in the PBOO only group when compared with the sham-operated group, and decreased in the PBOO relief group when compared with the PBOO group (P < 0.001). From the quantitative PCR and the western blot analyses, expression of Bax, caspase-3, P38, and Jnk was significantly increased in the PBOO group (P < 0.001). However, expression of Erk, Bcl-2 significantly decreased in the PBOO group (P < 0.001). The expression of Erk and Bcl-2 significantly increased in the PBOO relief group when compared with the PBOO group (P < 0.001). In comparison to the sham-operated group, expression levels of survivin significantly increased in both the PBOO and PBOO plus relief groups (P < 0.001). In addition, the expression levels were significantly different between the PBOO and PBOO plus relief groups (P < 0.001).

Conclusion: PBOO induced apoptosis of urothelium is related to alterations in the MAPK signaling pathways and apoptosis-related protein change. These results may also suggest that the pro-survival Erk signaling cascade and the expression survivin are activated in response to ischemic bladder injury and associated with initiation of bladder restoration in PBOO and subsequent relief. However, the mechanism of survivin as anti-apoptotic protein in ischemic bladder injuries remains unclear.

Background: Obturator nerve reflex is the surgery treatment side effect in patients with bladder cancers. This study is run to determine the obturator Nerve reflex by Thulium laser versus monopolar Transurethral Resection of Bladder Tumors (TURBT).


Materials and methods: After clinical trial approval code receiving IRCT20190624043991N4, one hundred and eighty-nine patients with bladder tumors from 2010 to 2016 were assessed and among them, 35 patients were randomly assigned into two groups in a blinded manner; the first group (16 patients) received thulium laser and the second group (19 patients) were patients undergoing monopolar transurethral resection of bladder tumor after spinal analgesia. Clinical data, including different variables such as; age, tumor characteristics, gender, operation duration, types of leg jerking, and some intraoperative complications, were recorded. The site of the obturator nerve was determined by nerve stimulation, anatomical landmarks, and ultrasonography. Leg jerking was compared in both groups.


Results: Of the 35 patients, 28 cases were male and 7 cases were female. The mean age was 62.0±6.9 (40-75) years in the Thulium laser group and 64.0±7.1 (41-77) years in the monopolar TURBT group. The mean operation time was no different between the two groups significantly (P > 0.05). Leg jerking was reported in 25% and 63.1% of the patients in Thulium laser and monopolar TURBT groups, respectively (P<0.05).


Conclusion:  Thulium laser is a more feasible and effective method to prevent leg jerking in patients with bladder cancer; so, it is recommended more than monopolar Transurethral.

Role of p-ERK1/2 in Benign Prostatic Hyperplasia during Hyperinsulinemia

Yong-Zhi Li, Ben-Kang Shi, Jing-Yu Li, Xing-Wang Zhu, Jia Liu, Yi-Li Liu

Urology Journal, 2019, 5 January 2019 , Page 5694
https://doi.org/10.22037/uj.v16i7.5694

Purpose: Using a rat model of hyperinsulinemia, the present study investigated the role of p-ERK1/2 in benign prostatic hyperplasia (BPH).


Materials and Methods: Forty male Sprague-Dawley rats were randomly selected and assigned to four groups: high fat diet (HFD)+BPH (n=10), HFD (n=10), BPH (n=10), and control (n=10) groups. Hyperinsulinemia was induced by HFD feeding, while BPH was induced using testosterone propionate. Plasma glucose, plasma insulin and bodyweight were examined weekly. Immunohistochemistry (IHC) and western blot analysis were used to analyze the expression of ERK1/2 and p-ERK1/2 in rat prostates.


Results: Plasma glucose and plasma insulin levels were significantly greater in the HFD+BPH and HFD groups, when compared to the other two groups (P<0.05). Prostate weights were significantly greater in the HFD+BPH, HFD and BPH groups, than in the control group (P<0.05). IHC and western blot analysis revealed that p-ERK1/2 expression was greater in the HFD+BPH group than in the other three groups (P<0.05).


Conclusion: Androgens plus a hyperinsulinemic condition induced by HFD can result in prostatic cell hyperplasia, and this mechanism may be correlated to the upregulation of p-ERK1/2. Further investigations of this possibility are required.

Purpose: To explore the association between 5α-reductase inhibitors (5ARIs) use and risk of depression based on published literature through a meta-analysis.


Materials and methods: A comprehensive literature search was conducted by searching Pubmed, Embase, Cochrane Library, CBM, CNKI and VIP databases up to June, 2019. Summarized risk ratios (RRs) with 95% confidence intervals (CIs) were calculated to evaluate the strength of association between 5ARIs and depression. Subgroup analyses were performed according to population, 5ARI types, degree of depression, and publication date. Registered in PROSPERO under number CRD42018096147.


Results: A total of 6 clinical studies with 265672 participants were included in our meta-analysis. The application of 5ARIs could significantly increase the risk of depression based on both pooled unadjusted (95% CI: 1.28-2.78, RR = 1.89, P = .001) and multivariable adjusted RRs (95% CI: 1.01-1.17, RR = 1.09, P = .03). In subgroup analyses, dutasteride was associated with depression significantly (95% CI: 1.37-1.70, RR = 1.53, P < .001), while finasteride was not. As to the degree of depression, 5ARIs mainly caused mild depression (95% CI: 1.91-2.33, RR = 2.11, P < .001), instead of moderate or severe depression.


Conclusion: We concluded that 5ARIs could potentially increase the risk of depression. Clinicians need to carefully consider the use of 5ARIs for benign prostatic hyperplasia and androgenic alopecia patients, especially those exhibiting risk factors for depression or those who have a previous history of depression. More studies with larger sample size and comprehensive study design are needed necessarily to further verify our outcomes.

Purpose: Intravesical BCG (Bacillus Calmette–Guérin) therapy is indicated as an effective treatment for patients with non-muscle-invasive bladder cancer, despite associate with the side effects. In this study, the incidence of BCG therapy adverse effects was compared among three groups of patients who received celecoxib, phenazopyridine, and oxybutynin with placebo.


Materials and Methods: The randomized controlled clinical trial was conducted on four groups using the parallel group method. A checklist is used for weekly assessment of urinary symptoms, systemic symptoms of BCG therapy, and adverse drug reactions.


Results: The study included 120 patients, 10 female and 110 male. The mean age 59.65 ± 6.2 years. The results of multivariate analysis show that there is a significant decrease in urinary frequency for patients who received phenazopyridine (95% CI: 0.09, 0.31, OR = 0.17, P <.001) and also celecoxib group (95% CI: 0.10, 0.43, OR = 0.21, P <.001) compared to those in placebo group. Patients in celecoxib group (95% CI: 0.02, 0.07 ,OR = 0.04, P <.001), phenazopyridine (95% CI : 0.07, 0.37,OR=0.16, P <.001) and oxybutynin (95% CI: 0.02, 0.12,OR = 0.05, P <.001) were less likely to have urgency than those in placebo. Moreover, significant decrease was found for dysuria in the three treatment groups in comparison with placebo group.


Conclusion: According to the results, celecoxib, phenazopyridine and oxybutynin can effectively decrease the side effects of BCG immunotherapy compared to placebo. Among these three treatments, the most effective and safest treatment option is celecoxib.

Purpose: To determine the empirical usage of antibiotics and analyze the pathogen spectrum during the perioperative period of flexible ureteroscopic lithotripsy (FURSL) with a focus on levofloxacin.


Materials and Methods: This retrospective analysis included 754 patients who underwent FURSL successfully in our hospital from January 2015 to July 2019. All patients were indicated urine cultures and prescribed antibiotics during the perioperative period. Patients with negative preoperative urine cultures were divided into levofloxacin (LVXG) and non-levofloxacin groups (NLVXG) based on the empirical use of antibiotics. Operative time, the length of postoperative hospital stays and total hospital stays, total hospitalization costs, postoperative fever rate and removal rate of stones were compared. Patients with positive urine cultures were analyzed for  pathogen distribution and antibiotic resistance.


Results: In the empirical use of antibiotics among 541 cases with negative urine cultures, the prescription rate of levofloxacin was 68.95%. Compared to that in NLVXG, LVXG had a lower cost of antibiotics but higher postoperative fever rate and longer hospital stay. There were no significant differences in operative time, the total hospitalization costs and the removal rate of stones between the two groups. The top two common pathogens were Escherichia coli (36.11%) and Enterococcus faecalis (24.07%), with resistance rates of 74.36% and 71.15% to levofloxacin, respectively.


Conclusion: Levofloxacin might be no longer suitable as the first-line choice of clinical experience when performing FURSL in our center.

A Comparative Study on the Clinical Efficacy of Modified Circumcision and Two Other Types of Circumcision

Quanxin Su, Shenglin Gao, Jiasheng Chen, Lu Chao, Mao Weijiang, Wu Xingyu, Lifeng Zhang, Li Zuo

Urology Journal, 2019, 5 January 2019 , Page 6193
https://doi.org/10.22037/uj.v16i7.6193

Purpose: To compare the clinical effects of three methods of circumcision: modified circumcision, traditional circumcision, and disposable suturing device circumcision.


Materials and Methods: Male patients (n = 241) with redundant prepuce and/or phimosis were included in a clinical trial from January 2019 to March 2020. Patients were divided into 3 groups based on the surgical method: group A, traditional circumcision (n = 79); group B, modified circumcision (n = 80); and group C, disposable suturing device circumcision (n = 82).


Results: The operation times in groups A, B, and C were 25.2 ± 3.3 min, 10.2 ± 2.7 min, and 6.7 ± 1.4 min, respectively. The volumes of intraoperative blood loss in groups A, B, and C were 12.7 ± 2.3 mL, 8.1 ± 3.4 mL, and 2.2 ± 0.8 mL, respectively (P < 0.05). Groups A and B were superior to group C in terms of the 6-h postoperative visual analog scale score and appearance satisfaction (P < 0.05). There were no obvious differences in the 7-day postoperative pain score and total healing time (P > 0.05). The operating expenses in groups A and B were lower than that in group C (P < 0.05).


Conclusion: Modified circumcision, with its advantages of shorter operation time, less blood loss and pain, lower cost, and better postoperative penile appearance, is easily accepted by patients and deserves wide clinical application.

Video


A Step-by-Step Guide to Double-Puncture Technique for Endoscopic Management of Ureterocele

Behnam Nabavizadeh, Reza Nabavizadeh, Abdol-Mohammad Kajbafzadeh

Urology Journal, 2019, 5 January 2019 , Page 6028
https://doi.org/10.22037/uj.v16i7.6028

To date, the optimal surgical technique for treatment of ureterocele remains unclear and the available options are variable. The endoscopic techniques that are gaining popularity mostly share major drawbacks including low success rate, high probability of mandatory secondary surgery and de novo vesicoureteral reflux to the ureterocele moiety. The Double-Puncture technique is shown to have promising outcomes in terms of long-term success and low rate of complications. In this video, a step-by-step guide to this technique is presented.