ISSN: 1735-1308

Instant 2019&2020


Cytotoxic Effect of Saffron Stigma Aqueous Extract on Human Prostate Cancer and Mouse Fibroblast Cell Lines

Hassan Ahmadnia, Jamshid Tabeshpour, Mehdi Younesi Rostami, Ehsan Mansourian, Alireza Akhavan Rezayat, Azam Brook

Urology Journal, , 1 January 2020 , Page 6331

Purpose: Several lines of experimental evidence have shown that saffron has anticarcinogenic effects. This study aimed at evaluating the possible anticancer effect of saffron stigma aqueous extract on human prostate cancer (PC3) and mouse fibroblast cells (L929) as non-cancerous control cells.

Materials and Methods: Saffron stigma aqueous extract at concentrations of 100, 200, 400, 600, 800, 1600 and 3200 μg/mL were prepared. PC3 and L929 cells were incubated with different concentrations of saffron extracts in different time intervals (24, 48, 72, 96 and 144 hours). MTT assay was used for each cell line to investigate the cytotoxic effect of saffron. Morphological alterations were also observed under light inverted microscope.

Results: In fibroblast cell line after 24 hours, Saffron extract did not affect significantly the normal cells and they were intact in morphologic view. After 96 hours in the cells with highest concentration (1600 μg/mL), cell death and cellular form changes as well as severe granulation was observed. In prostate cell line after 24 hours, the only changes were observed in cells with the concentration of 1600 μg/mL. The cells were granulated and the form of the cells were spherule. After 72 hours, in group with the concentration of 1600 μg/mL, severe granulation was observed and the cell count decreased and some cells were dead.

Conclusion: Saffron aqueous extract has an in vitro inhibitory effect on the proliferation of human prostate cell and mouse L929 cells which is dose-dependent.


IS there a Difference in Platelet-Rich Plasma Application Method and Frequency to Protect Against Urethra Stricture?

Arif Aydin, Mehmet Giray Sonmez, Pembe Oltulu, Rahim Kocabas, Leyla Ozturk Sonmez, Hakan Hakki Taskapu, Mehmet Balasar

Urology Journal, , 1 January 2020 , Page 6100

Material-Method:  Fifty rats were randomized into 5 groups, with 10 rats in each group. The urethras of all rats were traumatized with a pediatric urethrotome knife at 6 and 12 o’clock, except in the sham group. Group 1 was the sham group and had only urethral catheterization daily for 15 days, Group 2 was given 0.9% saline (physiologic saline [(UI+PS]) once a day after urethral injury (UI+ PS), Group 3 was injected with PRP submucosally after urethral injury, Group 4 was given PRP once a day as intraurethral instillation using a 22 Ga catheter sheath with urethral injury, and Group 5 was given PRP twice a day as intraurethral instillation using a 22 Ga catheter sheath with urethral injury.

Each administration of PRP was administered as 300 million platelets/150 microliters. On day 15, the penises of the rats were degloved to perform penectomy. Histopathologic evaluation was made for spongiofibrosis, inflammation, and congestion in vascular structures.

Results: When the sham group, UI+PS, UI+PRPx1, UI+PRPx2 and UI+PRPs groups are compared in total, there were significant differences identified for parameters other than edema. When the UI+PS, UI+PRPx1, UI+PRPx2 and UI+PRPs groups are compared, the UI+PS group was observed to have significantly more inflammation (mucosal inf. 2.42 ±0.53) and spongiofibrosis (2.42 ±0.53). All the PRP groups were identified to have significantly less mucosal inflammation (UI+PRPs 1±0, UI+PRPx1; 1.4±0.51, PRPx2; 1.33±0.5) and spongiofibrosis (UI+PRPs; 1.57±0.53, PRPx1; 1.2±0.42, PRPx2; 1.55±0.52). The group with lowest spongiofibrosis was seen to be the PRPx1 group.

Conclusion: This study showed that PRP significantly reduced mucosal inflammation and spongiofibrosis, independent of administration route, when applied to the urethra after urethral trauma


Current Trends in Hypospadias Repair. Where are we Standing?

Parisa Saeedi Sharifabad, Vahid Poudineh, Mehran Hiradfar, Ahmad Mohammadipour, Reza Shojaeian

Urology Journal, , 1 January 2020 , Page 6081

Purpose: Although several surgical approaches are suggested for hypospadias reconstruction but there is not a consensus on the best surgical method of hypospadias repair. This is a survey about technique of choice in treatment of various types of hypospadias.

Method and materials: We asked about preferred method of treatment for three different hypospadias scenarios. 170 surgeons completed the questionnaire and enrolled in this study.

Results: 51 general urologists, 61 pediatric surgeon, 16 plastic surgeons and 42 pediatric urologists joined the survey. Preferred method of surgery for a distal hypospadias was TIP in 53.5%. TIP was offered by 39.4% , Two stage Urethroplasty in 24.7% and Island On lay Flap in 21.2% to repair a proximal hypospadias without cordee. Two-stage Bracka or Durham Smith were common surgical options in proximal hypospadias surgery with cordee.

Conclusion: A growing trend was detected on TIP repair of distal and proximal hypospadias without cordee.


A Investigating the Outcome of Surgery in Patients with Penile Fracture

Hamid Mazdak, Hanieh Salehi, Zahra Tolou Ghamari, Reza Kazemi

Urology Journal, , 1 January 2020 , Page 6347


The aim of study was to investigate the outcome of surgery in patients with penile fracture in Al-Zahra hospital.

Materials and Methods

This cross sectional study was conducted on 187 patients with penile fracture underwent surgery in Al-Zahra hospital during 2016- 2020. Data such as penile fracture causes, erectile dysfunction, time of surgery after penile fracture, degree of penile curvature and etc were extracted from medical records.


The most common reason of penile fracture in these patients was manipulation and trauma with frequency 70 (37.4%) and 69 patients (36.9%), respectively. Lower urinary tract symptom, urinary tract injury, penile curvature, penile nodule and erectile dysfunction were observed in 1 (0.54 %), 2(1.06 %), 76 (40.64%), 75 (40.1%), 43 (23%) patients, respectively. Mild and moderate erectile dysfunction was seen in 38 (88.3%) and 5 (11.62%) patients, respectively. There was significant relation between erectile dysfunction with degree of penile curvature, surgical time and size of defect (P<.01). Furthermore, significant relation was observed between penile nodules and suture type (P=.000).


According to findings, erectile dysfunction was observed in 23 % of patients; however most of these patients had mild erectile dysfunction. Moreover, erectile dysfunction was influenced by penile curvature, surgical time and size of defect. Therefore, early surgery and special attention to patients with severe penile curvature are proposed for prevention of erectile dysfunction in these patients.

Background: Obturator nerve reflex is the surgery treatment side effect in patients with bladder cancers. This study is run to determine the obturator Nerve reflex by Thulium laser versus monopolar Transurethral Resection of Bladder Tumors (TURBT).

Materials and methods: After clinical trial approval code receiving IRCT20190624043991N4, one hundred and eighty-nine patients with bladder tumors from 2010 to 2016 were assessed and among them, 35 patients were randomly assigned into two groups in a blinded manner; the first group (16 patients) received thulium laser and the second group (19 patients) were patients undergoing monopolar transurethral resection of bladder tumor after spinal analgesia. Clinical data, including different variables such as; age, tumor characteristics, gender, operation duration, types of leg jerking, and some intraoperative complications, were recorded. The site of the obturator nerve was determined by nerve stimulation, anatomical landmarks, and ultrasonography. Leg jerking was compared in both groups.

Results: Of the 35 patients, 28 cases were male and 7 cases were female. The mean age was 62.0±6.9 (40-75) years in the Thulium laser group and 64.0±7.1 (41-77) years in the monopolar TURBT group. The mean operation time was no different between the two groups significantly (P > 0.05). Leg jerking was reported in 25% and 63.1% of the patients in Thulium laser and monopolar TURBT groups, respectively (P<0.05).

Conclusion:  Thulium laser is a more feasible and effective method to prevent leg jerking in patients with bladder cancer; so, it is recommended more than monopolar Transurethral.


To date, the optimal surgical technique for treatment of ureterocele remains unclear and the available options are variable. The endoscopic techniques that are gaining popularity mostly share major drawbacks including low success rate, high probability of mandatory secondary surgery and de novo vesicoureteral reflux to the ureterocele moiety. The Double-Puncture technique is shown to have promising outcomes in terms of long-term success and low rate of complications. In this video, a step-by-step guide to this technique is presented.