Micromorphological Evaluation of Dentin Treated with Different Desensitizing Agents
Journal of Lasers in Medical Sciences,
Vol. 4 No. 3 (2013),
Introduction: The purpose of a desensitizing agent is a permanent coating or filling of dentin surface. Morphological analysis in vitro of this treated surface is essential to understand the interaction between desensitizing agent and hypersensitive dentin. The aim was to evaluate the morphology of four dentin surface treated with desensitizing agents.
Methods: This was an in vitro laboratory study, where fifteen specimens from extracted human premolars were obtained. The enamel was removed to expose the dentin surface, polished with silicon carbide abrasive papers and etched with 6% citric acid for 2 min. The specimens were randomly divided into 5 groups: G1 - without treatment (control) (C), G2 - fluoride varnish (FV), G3 - potassium oxalate (PO), G4 - 2-step self-etching adhesive system (AS), G5 - diode laser (DL). The specimens were cleaved in the lingualbuccal direction, prepared for analysis by Scanning Electron Microscope and the surface and interior of the dentinal tubules were observed at 1500× magnification.
Results: In the control group, the dentin etching promoted smear layer removal and exposure of dentinal tubules. In the group of fluoride varnish, a film was observed on the surface, with plugs of varnish into tubules. In the group of oxalate, partial obliteration of the tubular entrances was observed. In the group of the adhesive system, the tubules were obstructed through the formation of hybrid layer and a physical barrier on the surface. In the group of the diode laser, dentin melting and solidification with partial occlusion of dentinal tubules were observed.
Conclusions: All desensitizing agents evaluated demonstrated ability to modify the surface of dentin, with partial or total occlusion of dentinal tubules. Thus, it is suggested to do more clinical studies to verify the effectiveness of the findings.
- dentin desensitizing
- diode lasers
How to Cite
Absi EG, Addy M, Adams D. Dentine hypersensitivity. A study of the patency of dentinal tubules in sensitive and non-sensitive cervical dentine. J Clin Periodontol. 1987;14:280-4.
Canadian Advisory Board on Dentin Hypersensitivity. Consensus-Based Recommendations for the Diagnosis and Management of Dentin Hypersensitivity. J Can Dent Assoc. 2003;69(4):221-6.
Orchardson R, Gillam DG. Managing dentin hypersensitivity. J Am Dent Assoc. 2006;137;990-8.
Brannstrom M. The hydrodynamic theory of dentinal pain: sensation in preparations, caries, and the dentinal crack syndrome. J Endod. 1986;12(10):453-7.
Al-Sabbagh M, Brown A, Thomas MV. In-Office Treatment of Dentinal Hypersensitivity. Dent Clin North Am. 2009;53: 47-60.
Bartold PM. Dentinal hypersensitivity: a review. Aust Dent J. 2006;51:(3):212-8.
Dowell P, Addy M. Dentine hypersensitivity – a review Aetiology, symptoms and theories of pain production. J Clin Periodontol. 1983;10:341-50.
Arrais CAG, Chan DCN, Giannini M. Effects of desensitizing agents on dentinal tubule occlusion. J Appl Oral Sci. 2004;12(2):144-8.
Oda M, Matos AB, Liberti EA. Morfologia da dentina tratada com substâncias dessensibilizantes: avaliação através da microscopia eletrônica de Varredura. Rev Odontol Univ São Paulo. 1999;13:337-42.
Aranha ACC, Pimenta LAF, Marchi GM. Clinical evaluation of desensitizing treatments for cervical dentin hypersensitivity. Braz Oral Res. 2009;23(3):333-9.
Assis JS, Rodrigues LKA, Fonteles CSR, Colares RCR, Souza AMB, Santiago SL. Dentin Hypersensitivity After Treatment With Desensitizing Agents: A Randomized, Double-Blind, Split-Mouth Clinical Trial. Braz Dent J. 2011;22(2):157-61.
Kehua Q, Yingying F, Hong S, Menghong W, Deyu H, Xu F. A cross-sectional study of dentine hypersensitivity in China. Int Dent J. 2009;59(6):376-80.
Rees JS, Addy M. A cross-sectional study of dentine hypersensitivity. J Clin Periodontol. 2002;29:997-1003.
Fu B, Shen Y, Wang H, Hannig M. Sealing Ability of Dentin Adhesives/Desensitizer. Oper Dent. 2007;32(5):496-503.
Mordan NJ, Barber PM, Gillam DG. The dentine disc. A review of its applicability as a model for the in vitro testing of dentine hypersensitivity. J Oral Rehabil. 1997;24:148-56.
Pereira JC, Martineli ACBF, Tung MS. Replica of Human Dentin Treated With Different Desensitizing Agents: A Methodological SEM Study In Vitro. Braz Dent J. 2002;13(2):75-85.
Chidchuangchai W, Vongsavan N, Matthews B. Sensory transduction mechanisms responsible for pain caused by cold stimulation of dentine in man. Arch Oral Biol. 2007;52:154-60.
Ritter AV, Dias WL, Miguez P, Caplan DJ, Swift Jr EJ. Treating cervical dentin hypersensitivity with fluoride varnish A randomized clinical study. J Am Dent Assoc. 2006;137:1013-20.
Kumar NG, Mehta DS. Short-Term Assessment of the Nd:YAG Laser With and Without Sodium Fluoride Varnish in the Treatment of Dentin Hypersensitivity – A Clinical and Scanning Electron Microscopy Study. J Periodontol. 2005;76(7):1140-7.
Pereira JC, Segala AD, Gillam DG. Effect of desensitizing agents on the hydraulic conductance of human dentin subjected to different surface pre-treatments – an in vitro study. Dent Mater. 2005;21(2):129-38.
Orchardson R, Gillam DG. The Efficacy of Potassium Salts as Agents for Treating Dentin Hypersensitivity. J Orofac Pain. 2000;14(1):9-19.
Erdemir U, Yildiz E, Kilic I, Yucel T, Ozel S. The efficacy of three desensitizing agents used to treat dentin hypersensitivity. J Am Dent Assoc. 2010;141:285-96.
Yuan Y, Shimada Y, Ichinose S, Tagami J. Hybridization quality in cervical cementum and superficial dentin using current adhesives. Dent Mater. 2008;24(5):584-93.
Arrais CAG, Giannini M. Morphology and thickness of the diffusion of resin through demineralized or unconditioned dentinal matrix. Pesqui Odontol Bras. 2002;16(2):115-20.
Gholami GA, Fekrazad R, Esmaiel-Nejad A, Kalhori KAM. An Evaluation of the Occluding Effects of Er;Cr:YSGG, Nd:YAG, CO2 and Diode Lasers on Dentinal Tubules: A Scanning Electron Microscope In Vitro Study. Photomed Laser Surg. 2011;29(2):115–21.
Theodoro LH, Haypek P, Bachmann L, Garcia VG, Sampaio JE, Zezell DM, et al. Effect of Er:YAG and Diode Laser Irradiation on the Root Surface: Morphological and Thermal Analysis. J Periodontol. 2003;74(6):838-43.
Haypek P, Zezell DM, Bachmann L, Marques MM. Interaction Between High-power Diode Laser and Dental Root Surface. Thermal, Morphological and Biocompatibility Analysis. J Oral Laser Appl. 2006;6:101- 9.
Kreisler M, Al Haj H, Daubländer M, Götz H, Duschner H, Willershausen B, et al. Effect of Diode Laser Irradiation on Root Surfaces in Vitro. J Clin Laser Med Surg. 2002;20(2):63-9.
Zapletalová Z, Perina J Jr, Novotny R, Chmelícková H. Suitable Conditions for Sealing of Open Dentinal Tubules Using a Pulsed Nd:YAG Laser. Photomed Laser Surg. 2007;25(6):495-9.
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