The Effect of bisphenol A and Photobiomodulation Therapy on Autophagy-Related Genes Induction in Adipose Tissue-Derived Stem Cells Effects of Bisphenol A and Photobiomodulation Treatments on Autophagy
Journal of Lasers in Medical Sciences,
Vol. 13 (2022),
10 January 2022
,
Page e15
Abstract
Introduction: As adipose tissue-derived stem cells (ADSCs) can divide rapidly and be prepared noninvasively, they have extensively been used in regenerative medicine. On the other hand, a new method of therapy, known as photobiomodulation (PHT), has been used to treat many diseases, such as inflammatory conditions, wound healing and pain. Besides, exposure to chemical substances such as bisphenol A (BPA), at low levels, can lead to autophagy. This study investigated the effects of BPA and PHT on the expression of autophagy-related genes, including LC3, NRF2, and P62, in rat ADSCs as a model.
Methods: ADSCs isolation and purification were confirmed by immunocytochemistry (ICC). The cells were then treated with different concentrations of BPA and also subjected to PHT. Reverse transcription polymerase chain reaction (RT-PCR) was used for the evaluation of LC3, NRF2 and P62 gene expressions. Oil red O staining was used for adipogenic vacuole formation.
Result: ICC showed that the isolated cells were CD 49-positive but CD 31 and CD 34-negative. The viability test indicated that the number of live cells after 24 hours in the BPA groups at concentrations of 0, 1, 50, 100 and 200 μM was 100%, 93%, 81%, 72%, and 43% respectively. The difference in cell viability between groups 50, 100 and 200 μM was significant as compared with the control groups (P<0.05). Moreover, in the group with 1 μM concentration of BPA, the expressions of LC3, NRF2 and P62 genes were upregulated. However, in the treatment group at the concentration of 200 μM of BPA, the LC3 gene was expressed, but NRF2 and P62 genes were downregulated.
Conclusion: BPA and PHT induce autophagy and adiposeness in ADSCs in a dose-dependent manner.
- Adipose tissue-derived stem cells; Bisphenol A; Photobiomodulation; NRF2 gene; P62 gene
How to Cite
References
Shams Nooraei M, Noori-Zadeh A, Darabi S, Rajaei F, Golmohammadi Z, Abbaszadeh HA. Low Level of Autophagy-Related Gene 10 (ATG10) Expression in the 6-Hydroxydopamine Rat Model of Parkinson's Disease. Iran Biomed J. Jan 1 2018;22(1):15-21. doi:10.22034/ibj.22.1.15
Darabi S, Tiraihi T, Noori-Zadeh A, Rajaei F, Darabi L, Abbaszadeh HA. Creatine and retinoic acid effects on the induction of autophagy and differentiation of adipose tissue-derived stem cells into GABAergic-like neurons. Article. J Babol Univ Medical Sci. 2017;19(8):41-49.
Agarwal S, Yadav A, Tiwari SK, Seth B, Chauhan LK, Khare P, et al. Dynamin-related protein 1 inhibition mitigates bisphenol A-mediated alterations in mitochondrial dynamics and neural stem cell proliferation and differentiation. Article. J Biol Chem. 2016;291(31):15923-15939. doi:10.1074/jbc.M115.709493
Ohlstein JF, Strong AL, McLachlan JA, Gimble JM, Burow ME, Bunnell BA. Bisphenol A enhances adipogenic differentiation of human adipose stromal/stem cells. J Mol Endocrin. 2014;53(3):345- 53. doi:10.1530/jme-14-0052
Yousefi B, Zarghami N, Samadi N, Majidinia M. Peroxisome Proliferator-Activated Receptors and their Ligands in Cancer Drug- Resistance: Opportunity or Challenge. Anticancer Agents Med Chem. 2016;16(12):1541-1548. doi:10.2174/1871520616666160204112941
de Lima RDN, Vieira SS, Antonio EL, Camillo de Carvalho PT, de Paula Vieira R, Mansano BSDM, Ferreira de Arruda Junior D, et al. Low-level laser therapy alleviates the deleterious effect of doxorubicin on rat adipose tissue-derived mesenchymal stem cells. J photochem photobiol B, Biol. 2019;196:111512. doi:10.1016/j.jphotobiol.2019.1115
Min KH, Byun JH, Heo CY, Kim EH, Choi HY, Pak CS. Effect of Low-Level Laser Therapy on Human Adipose-Derived Stem Cells: In Vitro and In Vivo Studies. Aesthetic Plast Surg. 2015;39(5):778- 82. doi:10.1007/s00266-015-0524-6
Darabi S, Noori-Zadeh A, Rajaei F, Abbaszadeh HA, Bakhtiyari S, Roozbahany NA. SMER28 Attenuates Dopaminergic Toxicity Mediated by 6-Hydroxydopamine in the Rats via Modulating Oxidative Burdens and Autophagy-Related Parameters. Neurochem Res. 2018;43(12):2313-2323. doi:10.1007/s11064-018-2652-2
Nakamura M, Yamanaka H, Oguro A, Imaoka S. Bisphenol A induces Nrf2-dependent drug- metabolizing enzymes through nitrosylation of Keap1. Drug Metab Pharmacokinetics. 2018;33(4):194-202. doi:10.1016/j.dmpk.2018.04.003
Zhang Y, Han L, Yang H, Pang J, Li P, Zhang G, et al. Bisphenol A affects cell viability involved in autophagy and apoptosis in goat testis sertoli cell. Environ Toxicolo Pharmacol. 2017;55:137- 147. doi:10.1016/j.etap.2017.07.014
Mahemuti L, Chen Q, Coughlan MC, Qiao C, Chepelev NL, Florian M, et al. Bisphenol A induces DSB-ATM-p53 signaling leading to cell cycle arrest, senescence, autophagy, stress response, and estrogen release in human fetal lung fibroblasts. Arch Toxicol. 2018;92(4):1453-1469. doi:10.1007/s00204-017-2150-3
Yang S, Zhang A, Li T, Gao R, Peng C, Liu L, et al. Dysregulated Autophagy in Hepatocytes Promotes Bisphenol A-Induced Hepatic Lipid Accumulation in Male Mice. Endocrin. 2017;158(9):2799-2812. doi:10.1210/en.2016-1479
Müller SG, Jardim NS, Quines CB, Nogueira CW. Diphenyl diselenide regulates Nrf2/Keap-1 signaling pathway and counteracts hepatic oxidative stress induced by bisphenol A in male mice. Environ Res. Jul 2018;164:280-287. doi:10.1016/j.envres.2018.03.006
Darabi S, Tiraihi T, Delshad A, Sadeghizadeh M, Khalil W, Taheri T. In vitro non-viral murine pro-neurotrophin 3 gene transfer into rat bone marrow stromal cells. J Neurol Sci.2017;375:137-145.doi:10.1016/j.jns.2017.01.058
Vom Saal FS, Nagel SC, Coe BL, Angle BM, Taylor JA. The estrogenic endocrine disrupting chemical bisphenol A (BPA) and obesity. Mol Cellular Endocrin. 2012;354(1-2):74-84. doi:10.1016/j.mce.2012.01.001
Rubin BS, Murray MK, Damassa DA, King JC, Soto AM. Perinatal exposure to low doses of bisphenol A affects body weight, patterns of estrous cyclicity, and plasma LH levels. Environ Health Perspect. Jul 2001;109(7):675-80. doi:10.1289/ehp.01109675
Masuno H, Iwanami J, Kidani T, Sakayama K, Honda K. Bisphenol a accelerates terminal differentiation of 3T3-L1 cells into adipocytes through the phosphatidylinositol 3-kinase pathway. Toxicol. Sci. TOXICOL SCI. 2005;84(2):319-27. doi:10.1093/toxsci/kfi088
Naik P, Vijayalaxmi KK. Cytogenetic evaluation for genotoxicity of bisphenol-A in bone marrow cells of Swiss albino mice. Mut Res. 2009;676(1-2):106-12. doi:10.1016/j.mrgentox.2009.04.010
Linehan C, Gupta S, Samali A, O'Connor L. Bisphenol A-mediated suppression of LPL gene expression inhibits triglyceride accumulation during adipogenic differentiation of human adult stem cells. PloS one. 2012;7(5):e36109. doi:10.1371/journal.pone.0036109
Ahrabi B, Tavirani MR, Khoramgah MS, Noroozian M, Darabi S, Khoshsirat S, et al. The Effect of Photobiomodulation Therapy on the Differentiation, Proliferation, and Migration of the Mesenchymal Stem Cell: A Review. J lasers Medical Sci. 2019;10(Suppl 1):S96-s103. doi:10.15171/jlms.2019.S17
Khoshsirat S, Abbaszadeh HA, Khoramgah MS, Darabi S, Mansouri V, Ahmady-Roozbahany , et al. Protective effect of Photobiomodulation Therapy and Bone Marrow Stromal Stem Cells Conditioned Media on Pheochromocytoma Cell Line 12 Against Oxidative Stress Induced by Hydrogen Peroxide. J lasers Medical Sci. 2019;10(3):163-170. doi:10.15171/jlms.2019.26
- Abstract Viewed: 547 times
- PDF Downloaded: 363 times