Phagocytosis of yeasts by lipopolysaccharide (LPS)-stimulated macrophages Yeast ingestion by LPS-stimulated macrophages
Student Research in Translational Medicine,
Vol. 5 (2023),
30 December 2023
https://doi.org/10.22037/smsj.v5.40522
Abstract
For phagocytosis measurement, 5×105 macrophages were seeded in a 12-well plate. 20 ng/ml LPS was added to cells to induce the M1 phenotype. After 24h the medium was changed. A suspension of heat-killed baker’s yeast was prepared at 108 particles per ml in DMEM medium. The yeast suspension was added to macrophages at a ratio of 1:10 (macrophage: yeast). Macrophages were allowed for 60 minutes to phagocyte the particles at 37°C. To remove the free yeasts, the well was washed with PBS. The phagocytosis was observed using an inverted microscope.
- Macrophages
- Phagocytosis
- Yeast
- Lipopolysaccharides
- M1 Polarization
How to Cite
References
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