Evaluation of Wound Healing Properties of Dryopteris filix-mas Leaf and Root Extracts on Albino Rats Wound Healing Potentials of Dryopteris filix-mas on Albino Rats
Iranian Journal of Pharmaceutical Sciences,
Vol. 17 No. 1 (2021),
15 January 2021
,
Page 79-90
https://doi.org/10.22037/ijps.v17.40301
Abstract
Dryopteris filix-mas (D. filix-mas), belonging to the family of Dryopteridacea is a swampy fern that is popularly used by the Southern Nigerian dwellers in the treatment of wounds, hemorrhages, boil and other diseases. In order to authenticate its folkloric benefits in wounds, this study evaluated its wound healing activity using excision model. A total of fifty (50) Wistar rats were randomized into ten groups of five animals each. After creation of surface wounds, group 1 received paraffin base (control). Group 2 received Povidon iodine (standard). Groups 3, 4, 5 and 6 received 1.25, 2.5, 5 and 10% (w/w) of an ethanol leaf extract of D. filix-mas formulated with paraffin base respectively. Groups 7, 8, 9, 10 were treated with 1.25, 2.5, 5 and 10% (w/w) of ethanol root extract D. filix-mas formulated with paraffin base respectively. Treatments were topically applied to wounds once daily and healing rate was monitored every 3 days for 21 days. Wound swaps were taken on day 10th and day 20th for bacteria load determination. In-vitro antimicrobial activities of the leaf and root extract were tested against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli using agar-well diffusion method. Higher concentrations (5 and 10%) of the leaf and root extracts exhibited better wound healing activities more than lower concentrations. The leaf extract produced a better healing rate (wound contraction), antimicrobial activity and body weight regaining activities more than the root extract. This study validates the traditional use of D filix-mas in the treatment of wounds.
- Antimicrobial activity
- Dryopteris filix-mas
- Leaf extract
- Root extract
- Wound healing
- Wistar rats
How to Cite
References
[2] Agyare C, Boakye YD, Bekoe EO, Hensel A, Dapaah SO and Appiah T. Review: African medicinal plants with wound healing properties. J. Ethnopharmacol. (2016) 177:85–100.
[3] Abbasi AM, Khan MA, Ahmad M, Zafar M, Jahan S and Sultana S. Ethnopharmacological application of medicinal plants to cure skin diseases and in folk cosmetics among the tribal communities of north-west Frontier province. Pakistan J. Ethnopharmacol. (2010) 128: 322–35.
[4] Solanki R and Nagori BP. A review on microorganisms causing wound infections on skin. Asian J. Pharm. Tech. (2013) 3(3): 119–22.
[5] Builders PF and Builders MI. Wound Care: Traditional African Medicine Approach. Intech open (2016) http://dx.doi.org/10.5772/65521. Accessed on June 5th, 2020.
[6] Adigun IA, Rahman GA, Yusuf IF and Ofoegbu CKF. The point prevalence and cost of wound management in a Nigerian teaching hospital. Nig. Med. J. (2010) 51(1): 23–25.
[7] Zhu JJ, Yao S, Guo X, Yue BS, Ma XY and Li J. Bioactivity-Guided Screening of Wound-Healing Active Constituents from American Cockroach (Periplaneta americana). Molecules. (2018) 23(1): 101.
[8] Wilson RW and Francis KW. Evaluation of Wound Healing Activity of Ethanolic Extract of Leaves of Croton Megalocarpus Using Excision Wound Model on Wister Albino Rats. IJSR. (2016) 4(3): 182-94.
[9] Piriz MA, Lima CAB, Jardim VMR, Mesquita MK, Souza ADZ and Hecck RM. Medicinal plants in the wound healing process: a literature review. Rev. Bras. Plantas Med. (2014) 16(3): 628-36.
[10] Marume A, Matope G, Katsande S and Khoza S. Wound Healing Properties of Selected Plants Used in Ethnoveterinary Medicine. 2017; 8(September), 1–10. https://doi.org/10.3389/fphar.2017.00544.
[11] Hadagali MD and Chua LS. The anti-inflammatory and wound healing properties of honey. Eur. Food. Res. Tech. (2014) 239(6): 1003–14.
[12] Nwosu MO. „Ethnobotanical studies on some pteridophytes of Southern Nigeria‟, Economic Botany (2002) 56(3): 255–59.
[13] Duke JA. Handbook of medicinal herbs, Herbal Reference Library. 2nd ed. CRC Press, Florida USA (2001) 677.
[14] Sekendar AM, Mostafa K, Raihan MO, Rahman MK, Hossain MA and Alam MS. Antioxidant and cytotoxic activities of methanolic extract of D. filix-mas (L.) Schott leaves. Int. J. Drug Dev. Res. (2012) 4(2): 223-29.
[15] Mohammed ASM, Khan MMRL, Jabin SA, Abedin N, Islam MF and Shaha B. „Nutritional quality and safety aspects of wild vegetables consume in Bangladesh. Asian Pac. J. Trop. Biomed. (2016) 6(2): 125–31.
[16] Batsatsashvili K, Mehdiyeva N, Kikvidze Z, Khutsishvili M, Sikharulidze S, Tchelidze D, Alizade V, Narel Y, Zambrana P and Rainer W. Dryopteris filix – mas (L) Schott Dryopteridaceae. Bussmann Springer International Publishing AG. R.W. Bussmann (ed.), Ethnobotany of the Caucasus, European Ethnobotany. (2016), DOI 10.1007/978- 3-319-50009-6_87-1. Accessed on June 5th, 2020.
[17] Marc EB, Nelly A, Annick DD and Frederic D. Plants used as remedies antirheumatic and antineuralgic in the traditional medicine of Lebanon. J. Ethnopharmacol. (2008): 120: 315–34.
[18] Uwumarongie HO, Enike MA and Bafor EE. Pharmacognostic Evaluation and Gastrointestinal Activity of Dryopteris filix-mas (L.) Schott (Dryopteridaceae). EJHCPR. (2016): 2: 19 – 25.
[19] Bafor EE, Omokaro WO, Uwumarongie OH, Elvis-Offiah UB, Omoruyi O, Viegelmann CV and Edrada-Ebel R. D. filix-mas (Dryopteridaceae) leaves inhibit mouse uterine activity. JOMPED. (2017) 1(1):1-12.
[20] Erhirhie EO, Ezemokwe ON and Ilodigwe E. Teratogenic Effects of Ethanol Leaf Extract of Dryopteris filix –mas (L.) Schott. AJNP. (2018) 6(1): 573-83.
[21] Erhirhie EO, Emeghebo CN, Ilodigwe EE, Ajaghaku DL, Umeokoli BO, Eze PM, Ngwoke KG and Okoye FBC. Dryopteris filix-mas (L.) Schott ethanolic leaf extract and fractions exhibited profound anti-inflammatory activity. Avicenna J. Phytomed. (2019) 9(4): 396-409.
[22] Yadav RNS, Agarwala M. Phytochemical analysis of some medicinal plants. J. Phytol. (2011) 3(12): 10-14.
[23] Okezie UM, Eze PM, Okoye FBC, Ikegbunam MN, Ugwu MC and Esimone CO. Biologically active metabolites of an endophytic fungus isolated from Vernonia amygdalin. AJOPRED (2017) 9(1): 24-26.
[24] Vinay K, Abdullah AK and Nagarajan K. Animal Models for the Evaluation of Wound Healing Activity. IBDR (2013): 3(5): 93-107. https://www.researchgate.net/publication 74010528_animal_models_for_the_evaluation_of_wound_healing_activity.
[25] Jian PS and Bari SB. Evaluation of wound healing effect of petroleum ether and methanolic extract of Abemoschus manihot, Medikik malvaceae, Wrightia tinctoria R. Br. Apocyanacae in rats. Braz. J. Pharmacogn. (2010) 20:156-271.
[26] Umeh VN, Ilodigwe EE., Ajaghaku DL, Erhirhie EO, Moke GE and Akah PA. Wound-healing Activity of the Aqueous Leaf Extract and Fractions of Ficus exasperata (Moraceae) and its Safety Evaluation on Albino Rats. J. Tradit. Complement. Med. (2014) 1(4): 246-52.
[27] Juríková T, Mlček J, Sochor J and Hegedűsová A. Polyphenols and their Mechanism of Action in Allergic Immune Response. Glob. J. Allergy. (2015) 1(2): 37-39.
[28] Iba Y, Shibata A, Kato M and Masukawa T. Possible involvement of mast cells in collagen remodeling in the late phase of cutaneous wound healing in mice. Int. Immunopharmacol. (2014) 4:1873–80.
[29] Soare LC, Ferdes M, Stefanov S, Denkova Z, Nicolova R, Denev P, Bejan C and Paunescu A. Antioxidant activity, polyphenols content and antimicrobial activity of several native Pteridophytes of Romania. Notulae Botanicae. Horti Agrobotanici Cluj Napoca. (2012) 40(1): 53-57.
[30] Atawodi SE, Atawodi JC, Idakwo GA, Pfundstein B, Haubner R, Wurtele G, Bartsch H and Owen RW. Evaluation of the polyphenol content and antioxidant properties of methanol extracts of the leaves, stem, and root barks of Moringa oleifera Lam. J. Med. Food. (2010) 13(3): 710–16.
- Abstract Viewed: 164 times
- IJPS_Volume 17_Issue 1_Pages 79-90 Downloaded: 50 times