Assessment of Sterility and Residual Solvents Agents in Antler-derived and Bovine Xenograft Bone Substitutes: An In-vitro Study
Journal of Dental School,
Vol. 40 No. 1 (2022),
15 January 2023
,
Page 26-30
https://doi.org/10.22037/jds.v40i1.40075
Abstract
Objectives This study aimed to assess and compare the results of sterility and residual solvent testing in a newly developed antler-derived xenograft versus a bovine-derived xenograft.
Methods First, test and control samples were prepared using thermal and chemical procedures, involving immersion in deionized water for 24 hours, drying, boiling in sterile water, chemical treatment with chloroform and methanol, and heating at 650°C in a furnace. Next, they were sterilized via gamma radiation at 25 kGy. The sterility test was then performed based on the ISO 11737-2:2019 standard, using the direct inoculation method. Finally, residual solvent testing was carried out via gas chromatography-mass spectrometry.
Results The sterility test showed no evidence of bacterial or fungal growth in any of the samples during 14 days of incubation. Also, residual solvent testing indicated no sign of residual solvents in the samples.
Conclusion Antler-derived xenograft was safe to use in terms of the sterility and removal of residual solvents. Further studies should be carried out regarding other important laboratory tests as well as the animal and clinical studies.
- Xenograft
- Bone substitute
- Deer antler
- Sterility testing
- Residual solvent testing
How to Cite
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