Comparison of Biocompatibility of Various Membranes with Fibroblastsy
Journal of Dental School, Shahid Beheshti University of Medical Sciences,
Vol. 32 No. 3 (2014),
13 March 2019
,
Page 145-150
https://doi.org/10.22037/jds.v32i3.24790
Abstract
Objective: Different techniques have been suggested for the repair of bone defects at the injured sites. Use of biomembranes, or application of plasma rich in growth factor (PRGF) at the site of proliferation of osteoblasts are among the suggested techniques. The current study aimed to compare the biocompatibility of human periodontal ligament fibroblasts (hPLF) cultured on Hypro-Sorb F, Pericardium and Tutodent resorbable membranes coated with PRGF.
Methods: This experimental study was conducted on four resorbable membranes namely Hypro- Sorb F, Pericardium, Tutodent and Vicryl. Fibroblast cells isolated from the periodontal ligament of premolar tooth were passaged three times and 105 cells/ cm2 were cultured on membranes coated with PRGF. After 72 hours, the cells were evaluated in terms of biocompatibility and alkaline phosphatase (ALP) activity. Statistical analysis was carried out using one-way ANOVA.
Results: PRGF increased cell adhesion and Tutodent membrane coated with PRGF showed the highest cell adhesion compared with Hypro-Sorb F and Pericardium membranes (p=0.005).
Conclusion: PRGF increases cell viability and ALP activity of cells on biomembranes. PRGF treatment increases the adhesion of fibroblast cells to these membranes.
- Alkaline phosphatase
- Fibroblast
- MTT
- PRGF
How to Cite
References
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