Effect of Zingiber Officinale (Ginger Rhizomes) Hydroethanolic Extract on Hyoscine-Induced Memory Impairment in Adult Male Rats
International Clinical Neuroscience Journal,
Vol. 2 No. 3 (2015),
30 December 2015
,
Page 105-110
https://doi.org/10.22037/icnj.v2i3.9829
Abstract
Background: The spice Zingiber officinale or ginger possesses antioxidant activity and
neuroprotective effects. In this study we hypothesized that treatment with hydroethanolic
extract of ginger (50, 100 and 200 mg/kg, p.o) would effect on the hyoscine-induced memory
impairment in rats.
Methods: In this experimental study 64 male Wistar rats were divided into eight groups (8 rats
in each group): normal saline, hyoscine (1 mg/kg), ginger extract (50, 100 and 200 mg/kg),
or hyoscine (1 mg/kg) plus ginger extract (50, 100 and 200 mg/kg). Memory impairment was
induced by a single injection of hyoscine (1 mg/kg, i.p). Cognitive functions were evaluated
using passive avoidance learning (PAL) task. Retention test was carried out 24 h after training,
and the latency of entering the dark compartment [step-through latency (STL)] and the total
time in the dark compartment (TDC) were recorded. All statistical analysis was carried out at
5% level of significance using SPSS version 21. The data were analyzed by ANOVA followed
by Tukey’s test.
Results: The time latency in hyoscine-treated group was lower than control (133.87±14.60
vs. 242.12±10.58; p<0.001, respectively). Treatment of the animals by 100 and 200 mg/kg of
ginger extract before the training trial increased the time latency at 24 h after the training trial
(277±4.67 and 280.37±7.68; p<0.01, respectively). Administration of both 100 and 200 mg/kg
doses of the extract in hyoscine received animal groups before retention trials also increased
the time latency than the hyoscine-treated groups (247.37±7.62 and 271.87±9.11; p<0.001,
respectively).
Conclusion: The results revealed that the ginger hydroethanolic extract attenuated hyoscineinduced
memory impairment.
- Memory
- Alzheimer
- Ginger
- Scopolamine
- Rat
How to Cite
References
Nie K, Yu JC, Fu Y, Cheng HY, Chen FY, Qu Y, et al. Agerelated
decrease in constructive activation of Akt/PKB in
SAMP10 hippocampus. Biochem Biophys Res Commun.
;378(1):103-7.
Orhan I, Aslan M. Appraisal of scopolamine-induced
antiamnesic effect in mice and in vitro antiacetylcholinesterase
and antioxidant activities of some traditionally used
Lamiaceae plants. J Ethnopharmacol. 2009;122(2):327-32.
Hasselmo ME. The role of acetylcholine in learning and
memory. Curr Opin Neurobiol. 2006;16:710-715.
Chang Q, Gold PE. Switching memory systems during
learning: changes in patterns of brain acetylcholine release
in the hippocampus and striatum in rats. J Neurosci.
;23:3001-3005.
Klinkenberg I, Blokland A. The validity of scopolamine
as a pharmacological model for cognitive impairment: A
review of animal behavioral studies. Neurosci Behav Rev.
;34:1307-1350.
Francis ST, Head K, Morris PG, Macdonald IA. The effect
of flavanol-rich cocoa on the fMRI response to a cognitive
task in healthy young people. J Cardiovasc Pharmacol.
;47:215-220.
Buccafusco JJ. The revival of scopolamine reversal for
assessment of cognition-enhancing drugs. In: Methods of
Behavior Analysis in Neuroscience edited by Buccafusco JJ
(2nd ed.). Boca Raton (FL): CRC Press; 2009. p. 230-329.
Oh SR, Kim SJ, Kim DH, Ryu JH, Ahn EM, Jung JW.
Angelica keiskei ameliorates scopolamine-induced memory
impairments in mice. Biol Pharm Bull. 2013;36(1):82-88.
doi: 10.1248/bpb.b12-00681.
Rasmussen P. Ginger-Zingiber officinale Roscoe,
Zingiberaceae. J Prim Health Care. 2011;3(3):235-6.
Hosseini A, Mirazi N. Acute administration of ginger (Zingiber officinale rhizomes) extract on timed
intravenous pentylenetetrazol infusion seizure model in
mice. Epilepsy Res. 2014;108(3):411-9. doi: 10.1016/j.
eplepsyres.2014.01.008.
Joshi H, Parle M. Zingiber officinale: Evaluation of its
Nootropic effect in mice. Afr J Trad CAM 2006;3(1):64-74.
Felipe CFB, Fonsêca KS, dos Reis Barbosa AL, Bezerra
JNS, Neto MA, de Franca Fonteles MM, et al. Alterations
in behavior and memory induced by the essential oil of
Zingiber officinale Roscoe (ginger) in mice are cholinergicdependent.
J Med Plants Res. 2008; 2(7):163-170.
Gomar A, Hosseini A, Mirazi N. Preventive effect of
Rubus fruticosus on learning and memory impairment in
an experimental model of diabetic neuropathy in male rats.
PharmaNutrition. 2014;2:155-160.
Soodi M, Naghdi N, Hajimehdipoor H, Choopani S, Sahraei
E. Memoryimproving
activity of Melissa officinalis extract
in naïve and scopolaminetreated
rats. Res Pharm Sci.
;9(2):107-114.
Terry AV, Callahan PM, Hall B, Webster SJ. Alzheimer’s
disease and agerelated
memory decline (preclinical).
Pharmacol Biochem Behav. 2011;99:190-210. doi: 10.1016/j.
pbb.2011.02.002.
Bradford MM. A Rapid and sensitive method for the
quantitation of microgram quantities of protein utilizing the
principle of proteindye
binding. Anal Biochem. 1976;72:248-
Hodges DB, Jr, Lindner MD, Hogan JB, Jones KM, Markus
EJ. Scopolamine induced deficits in a battery of rat cognitive
tests: comparisons of sensitivity and specificity. Behav
Pharmacol. 2009;20:237-251.
Kucukatay V, Agar A, Gumuslu S, Yargiçoglu P. Effect
of sulfur dioxide on active and passive avoidance in
experimental diabetes mellitus: relation to oxidant stress
and antioxidant enzymes. Int J Neurosci. 2007;117:1091-107.
Bierer LM, Haroutunian V, Gabriel S, Knott PJ, Carlin LS,
Purohit DP, et al. Neurochemical correlates of dementia
severity in Alzheimer’s disease: relative importance of
the cholinergic deficits. J Neurochem. 1995;64:749-760.
Lever C, Burton S, O’Keefe J. Rearing on hind legs,
environmental novelty, and the hippocampal formation.
Rev Neurosci. 2006;17:111-133.
Grön G, Kirstein M, Thielscher A, Riepe MW, Spitzer M.
Cholinergic enhancement of episodic memory in healthy
young adults. Psychopharmacology (Berl). 2005;182:170-
Chan WK, Wong PT, Sheu FS. Frontal cortical alpha7 and
alpha4beta2 nicotinic acetylcholine receptors in working and
reference memory. Neuropharmacology. 2007;52:1641-1649.
Parikh V, Kozak R, Martinez V, Sarter M. Prefrontal
acetylcholine release controls cue detection on multiple
timescales. Neuron. 2007;56:141-154.
Conforti F, Statti GA, Tundis R, Loizzo MR, Menichini F. In
vitro activities of Citrus medica L. cv. Diamante (Diamante
citron) relevant to treatment of diabetes and Alzheimer’s
disease. Phytother Res. 2007;21:427-33.
Akhondzadeh S, Abbasi SH. Herbal medicine in the treatment
of Alzheimer’s disease. Am J Alzheimers Dis Other Dement.
;21:113-8.
Hasenohrl RU, Nichau CH, Frisch CH, De Souza Silva
MA, Huston JP, Mattern CM, et al. Anxiolytic-like effect
of combined extracts of Zingiber officinale and Ginkgo
biloba in the elevated plus-maze. Pharmacol Biochem
Behav. 1996;53:271-275.
Topic B, Hasenohrl RU, Hacker R, Huston JP. Enhanced
conditioned inhibitory avoidance by a combined extract
of Zingiber officinale and Ginkgo biloba. Phytother Res.
;16: 312-315.
Topic B, Tani E, Tsiakitzis K, Kourounakis PN, Dere E,
Hasenohrl RU, et al. Enhanced maze performance and
reduced oxidative stress by combined extracts of Zingiber
officinale and Ginkgo biloba in the aged rat. Neurobiol
Aging. 2002;23:135-143.
Grzanna R, Phan P, Polotsky A, Lindmark L, Frondoza
CG. Ginger extract inhibits beta-amyloid peptide-induced
cytokine and chemokine expression in cultured THP-1
monocytes. J Altern Complement Med. 2004;10:1009-1013.
Ghayur MN, Gilani AH, Ahmed T, Khalid A, Nawaz SA,
Agbedahunsi JM, et al. Muscarinic, Ca(++) antagonist and
specific butyrylcholinesterase inhibitory activity of dried
ginger extract might explain its use in dementia. J Pharm
Pharmacol. 2008;60(10):1375-83.
- Abstract Viewed: 610 times
- PDF Downloaded: 691 times