The Effect of Low Level Laser Therapy on Direct Pulp Capping in Dogs

Maryam Bidar; Siavash Moushekhian; Maryam Gharechahi; Ali Talati; Farzaneh Ahrari; Maryam Boujarpour

The Effect of Low Level Laser Therapy on Direct Pulp Capping in Dogs

Journal of Lasers in Medical Sciences, Vol. 7 No. 3 (2016), 17 July 2016 , Page 177-183
Published 17 July 2016

Abstract

Introduction: The aim of this study was to determine the histologic effect of low level laser therapy (LLLT) with or without the use of mineral trioxide aggregate (MTA) on exposed pulp tissues of dogs.

Methods: Twenty-five premolar teeth in three healthy mature dogs were randomly divided into five groups. In group 1, the pulp tissue was capped with MTA. In groups 2 and 3, before capping with MTA, the exposure site was irradiated by low power lasers at 630 nm (20 mW, 150 seconds, 7.5 J/cm2) and 810 nm (peak power 80 W, average power 50 mW, 1500 Hz, 50 seconds, 6.25 J/cm2) wavelengths, respectively. In groups 4 and 5, the exposure site was irradiated similar to that described in groups 2 and 3, but the pulp tissue was covered by gold foil instead of MTA. After 2 months, the animals were sacrificed and the samples were prepared for histologic evaluation.

Results: There were differences in pulpal response and dentinal barrier formation among the study groups. The morphology of pulpal tissue and the integrity of dentinal barrier and formation of odontoblastic layer were more favorable in the first three groups. The occurrence of extensive and intense inflammation and necrosis was more frequent in groups 4 and 5.

Conclusion: Under the conditions used in this study, the presence of MTA as a pulp capping material was more important than laser therapy in the success of direct pulp capping (DPC) treatment. MTA proved to be an effective material either alone or in combination with laser irradiation in vital pulp therapy.

Keywords:

Low level laser therapy
Vital pulp therapy
Dentinal bridge
Gold foil
MTA
Pulp capping
Pulp morphology

How to Cite

Bidar, M., Moushekhian, S., Gharechahi, M., Talati, A., Ahrari, F., & Boujarpour, M. (2016). The Effect of Low Level Laser Therapy on Direct Pulp Capping in Dogs. Journal of Lasers in Medical Sciences, 7(3), 177-183. Retrieved from https://journals.sbmu.ac.ir/jlms/article/view/9948

References

Ravindra SV, Mamatha GP, Sunita JD, Balappanavar AY, Sardana V. Morphometric analysis of pulp size in maxillary permanent central incisors correlated with age: An indirect digital study. J Forensic Dent Sci. 2015;7(3):208-214. doi:10.4103/0975-1475.172438.

Nowicka A, Wilk G, Lipski M, Kołecki J, Buczkowska- Radlińska J. Tomographic evaluation of reparative dentin formation after direct pulp capping with Ca (OH) 2, MTA, biodentine, and dentin bonding system in human teeth. J Endod. 2015;41(8):1234-1240. doi:10.1016/j. joen.2015.03.017.

Small B, Johnson W. Gold foil and its use in modern dentistry. Dent Today. 2006;25(3):92-96.

Tabarsi B, Parirokh M, Eghbal M, Haghdoost AA, Torabzadeh H, Asgary S. A comparative study of dental pulp response to several pulpotomy agents. Int Endod J. 2010;43(7):565-571. doi:10.1111/j.1365-2591.2010.01711.x.

Ahrari F, Madani AS, Ghafouri ZS, Tuner J. The efficacy of low-level laser therapy for the treatment of myogenous temporomandibular joint disorder. Lasers Med Sci. 2014;29(2):551-557. doi:10.1007/s10103-012-1253-6.

Ferreira AN, Silveira L Jr, Genovese WJ, et al. Effect of GaAIAs laser on reactional dentinogenesis induction in human teeth. Photomed Laser Ther. 2006;24(3):358-365. doi:10.1089/pho.2006.24.358.

Eshghpour M, Ahrari F, Najjarkar NT, Khajavi MA. Comparison of the effect of low level laser therapy with alvogyl on the management of alveolar osteitis. Med Oral Patol Oral Cir Bucal. 2015;20(3):e386-e392. doi:10.4317/ medoral.20375.

Moosavi H, Maleknejad F, Sharifi M, Ahrari F. A randomized clinical trial of the effect of low-level laser therapy before composite placement on postoperative sensitivity in class V restorations. Lasers Med Sci. 2015;30(4):1245-1249. doi:10.1007/s10103-014-1565-9.

Heravi F, Ahrari F, Mahdavi M, Basafa S. Comparative evaluation of the effect of Er:YAG laser and low level laser irradiation combined with CPP-ACPF cream on treatment of enamel caries. J Clin Exp Dent. 2014;6(2):e121-e126. doi:10.4317/jced.51309.

Heravi F, Moradi A, Ahrari F. The effect of low level laser therapy on the rate of tooth movement and pain perception during canine retraction. Oral Health Dent Manag. 2014;13(2):183-188.

De Coster P, Rajasekharan S, Martens L. Laser‐assisted pulpotomy in primary teeth: a systematic review. Int J Paediatr Dent. 2013;23(6):389-399.

Fernandes AP, Lourenço Neto N, Teixeira Marques NC, et al. Clinical and radiographic outcomes of the use of Low‐ Level Laser Therapy in vital pulp of primary teeth. Int J Paediatr Dent. 2015;25(2):144-150.

Marques NC Neto NL, de Oliveira Rodini C, et al. Low-level laser therapy as an alternative for pulpotomy in human primary teeth. Lasers Med Sci. 2015;30(7):1815-1822. doi:10.1007/s10103-014-1656-7.

Utsunomiya T. A histopathological study of the effects of low-power laser irradiation on wound healing of exposed dental pulp tissues in dogs, with special reference to lectins and collagens. J Endod. 1998;24(3):187-193. doi:10.1016/ s0099-2399(98)80181-7.

Asgary S, Eghbal MJ, Parirokh M, Ghanavati F, Rahimi H. A comparative study of histologic response to different pulp capping materials and a novel endodontic cement. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;106(4):609- 614. doi:10.1016/j.tripleo.2008.06.006.

Witherspoon DE. Vital pulp therapy with new materials: new directions and treatment perspectives—permanent teeth. J Endod. 2008;34(7):S25-S28. doi:10.1016/j. joen.2008.02.030.

Briso ALF, Rahal V, Mestrener SR, Dezan Junior E. Biological response of pulps submitted to different capping materials. Braz Oral Res. 2006;20(3):219-225. doi:10.1590/ s1806-83242006000300007.

Mestrener SR, Holland R, Dezan E. Influence of age on the behavior of dental pulp of dog teeth after capping with an adhesive system or calcium hydroxide. Dent Traumatol. 2003;19(5):255-261. doi:10.1034/j.1600-9657.2003.00167.x.

Lu Y, Liu T, Li X, Li H, Pi G. Histologic evaluation of direct pulp capping with a self-etching adhesive and calcium hydroxide in beagles. Oral Sur Oral Med Oral Pathol Oral Radiol Endod. 2006;102(4):e78-e84. doi:10.1016/j. tripleo.2006.02.020.

Ferriello V, Faria MR, Cavalcanti BN. The effects of low-level diode laser treatment and dental pulp-capping materials on the proliferation of L-929 fibroblasts. J Oral Sci. 2010;52(1):33-38. doi:10.2334/josnusd.52.33.

Yasukawa A, HRUI H, Koyama Y, Nagai M, Takakuda K. The effect of low reactive-level laser therapy (LLLT) with helium-neon laser on operative wound healing in a rat model. J Vet Med Sci. 2007;69(8):799-806. doi:10.1292/ jvms.69.799.

Maiya GA, Kumar P, Rao L. Effect of low intensity helium-neon (He-Ne) laser irradiation on diabetic wound healing dynamics. Photomed Laser Ther. 2005;23(2):187-90. doi:10.1089/pho.2005.23.187.

Madani AS, Ahrari F, Nasiri F, Abtahi M, Tuner J. Low-level laser therapy for management of TMJ osteoarthritis. Cranio. 2014;32(1):38-44. doi:10.1179/0886963413z.0000000004.

Suzuki M, Ogisu T, Kato C, Shinkai K, Katoh Y. Effect of CO2 laser irradiation on wound healing of exposed rat pulp. Odontology. 2011;99(1):34-44. doi:10.1007/s10266- 010-0140-5.

Suzuki M, Katsumi A, Watanabe R, Shirono M, Katoh Y. Effects of an experimentally developed adhesive resin system and CO2 laser irradiation on direct pulp capping. Oper Dent. 2005;30(6):702-718.

Moritz A, Schoop U, Goharkhay K, Sperr W. Advantages of a pulsed CO2 laser in direct pulp capping: a long-term. Lasers Surg Med. 1998;22:288-293.

Nammour S, Tielemans M, Heysselaer D, Pilipili Ch, De Moor R, Nyssen-Behets C. Comparative study on dogs between CO2 laser and conventional technique in direct pulp capping. Rev Belge Med Dent. 2008;64(2):81-86.

Olivi G, Genovese M, Maturo P, Docimo R. Pulp capping: advantages of using laser technology. Eur J Paediatr Dent. 2007;8(2):89-95.

Yazdanfar I, Gutknecht N, Franzen R. Effects of diode laser on direct pulp capping treatment. Lasers Med Sci. 2015;30(4):1237-1243.

Golpayegani MV, Ansari G, Tadayon N, Shams S, Mir M. Low-level laser therapy for pulpotomy treatment of primary molars. J Dent Tehran Univ Med Sci. 2009;6(4):168-174.

AlGhamdi KM, Kumar A, Moussa NA. Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci. 2012;27(1):237- 249. doi:10.1007/s10103-011-0885-2.

Jahanbin A, Ramazanzadeh B, Ahrari F, Forouzanfar A, Beidokhti M. Effectiveness of Er:YAG laser-aided fiberotomy and low-level laser therapy in alleviating relapse of rotated incisors. Am J Orthod Dentofacial Orthop. 2014;146(5):565-572. doi:10.1016/j.ajodo.2014.07.006.

Nadin G, Goel BR, Yeung CA, Glenny AM. Pulp treatment for extensive decay in primary teeth. Cochrane Database Syst Rev. 2003(1):CD003220. doi:10.1002/14651858. cd003220.

Matić M, Lazetić B, Poljacki M, Duran V, Ivkov-Simić M. Low level laser irradiation and its effect on repair processes in the skin. Med Pregl. 2002;56(3-4):137-141. doi:10.2298/ mpns0304137m.

Ezzati A, Bayat M, Khoshvaghti A. Low-level laser therapy with a pulsed infrared laser accelerates second-degree burn healing in rat: a clinical and microbiologic study. Photomed Laser Surg. 2010;28(5):603-611. doi:10.1089/ pho.2009.2544.

Neiburger E. Rapid healing of gingival incisions by the helium-neon diode laser. J Mass Dent Soc. 1998;48(1):8-13.

Abstract Viewed: 825 times
PDF Downloaded: 330 times