Effect of Nd:YAG Laser Irradiation on the Number of Open Dentinal Tubules and Their Diameter with and without Smear of Graphite: An in Vitro Study

Mohammad Reza Maleki-pour; Reza Birang; Maryam Khoshayand; Narges Naghsh

Effect of Nd:YAG Laser Irradiation on the Number of Open Dentinal Tubules and Their Diameter with and without Smear of Graphite: An in Vitro Study

Journal of Lasers in Medical Sciences, Vol. 6 No. 1 (2015), , Page 32-39
Published 29 December 2014

Abstract

Introduction: Dentin hypersensitivity (DH) is characterized by tooth pain arising from exposure of dental roots. In this study the efficiency of neodymium yttrium-aluminum-garnet (Nd:YAG) laser in association with graphite on dentinal surface changes as the alternative to the treatment of DH was evaluated.

Methods: Sixteen noncarious human third molars were collected and sectioned into 5 parts from cementoenamel junction (CEJ) to the furcation area. The prepared samples were randomly assigned into five groups (Gs) of each 16: Control (G1), treated by Nd:YAG laser at 0.5 W (G2), irradiation of Nd:YAG with a 0.25 W output power(G3), smeared with graphite and then using Nd:YAG laser at output powers of 0.5 W (G4) and 0.25 W (G5). For all groups the parameters were 15 Hz, 60 s, at two stages and with a right angle irradiation. The number and diameter of dentinal tubules (DT) were compared and analyzed by SPSS software, One way ANOVA and Post hoc LSD tests.

Results: The number of open dentinal tubules varied significantly between all groups except among G1 with G3 and G2 with G5. Multiple comparison tests also exhibited significant differences regarding the diameter of tubules between the groups two by two except among G2 with G5.

Conclusion: Nd:YAG laser used at 0.25 W and 0.5 W with application of graphite smear was able to reduce the number and diameter of dentinal tubules.

Keywords:

dentin hypersensitivity
Nd
YAG lasers
scanning electron microscopy.

How to Cite

Maleki-pour, M. R., Birang, R., Khoshayand, M., & Naghsh, N. (2014). Effect of Nd:YAG Laser Irradiation on the Number of Open Dentinal Tubules and Their Diameter with and without Smear of Graphite: An in Vitro Study. Journal of Lasers in Medical Sciences, 6(1), 32-39. Retrieved from https://journals.sbmu.ac.ir/jlms/article/view/6722

References

Marsilio AL, Rodrigues JR, Borges AB. Effect of the clinical application of the GaAlAs laser in the treatment of dentine hypersensitivity. J Clin Laser Med Surg. 2003; 21(5):291-6.

Rees JS, Addy M. A cross-sectional study of dentine hypersensitivity. J Clin Periodontol. 2002; 29(11):997-1003.

Chabanski MB, Gillam DG, Bulman JS, Newman HN. Prevalence of cervical dentine sensitivity in a population of patients referred to a specialist Periodontology Department. J Clin Periodontol. 1996; 23(11):989-92.

Trushkowsky RD, OquendoA. Treatment of dentin hypersensitivity. Dent Clin North Am. 2011; 55(3):599-608,

Ladalardo TC, Pinheiro A, Campos RA, Brugnera Júnior A, Zanin F, Albernaz PL, et al. Laser therapy in the treatment of dentine hypersensitivity. Braz Dent J. 2004; 15(2):144-50.

Brännstrom M, Aström A. A study of the mechanism of painelicited from the dentin. J Dent Rest. 1964; 63:619.

Mehta P, Vimala N, Mandke L. An insight into dentin desensitizing agents - In vivo study. Indian J Dent Res. 2013; 24:571-4

Khoroushi M, Keshani F. A review of glass-ionomers: From conventional glass-ionomer to bioactive glass-ionomer. Dent Res J (Isfahan). 2013; 10(4):411-20.

Wara-aswapati N, Krongnawakul D, Jiraviboon D, Adulyanon S, Karimbux N, Pitiphat W. The effect of a new toothpaste containing potassium nitrate and triclosan on gingival health, plaque formation and dentine hypersensitivity. J Clin Periodontol. 2005;32:53−8.

Petersson LG. The role of fluoride in the preventive management of dentin hypersensitivity and root caries. Clin Oral Investig. 2013; 17 Suppl 1:S63-71.

Moghareh Abed A, Naghsh N, Birang R, Shafiei F, Seifi M, Yaghini J. Clinical Evaluation of the Efficacy of Nd: YAG Laser Therapy and Sensikin® in Treatment of Dentine Hypersensitivity. J Lasers Med Sci. 2012; 3(2):61-6.

Miserendino LJ, Levy G, Miserendino CA. Laser interaction with biologic tissues. In: Miserendino LJ, Pick RM. Laser in Dentistry. Chicago: Quintessence, 1995: 39-56.

Matsumoto K, Funai H, Shirasuka T, Wakabayashi H. Effect of Nd:YAG‑Laser in treatment of cervical hypersensitive dentine. J Conserv Dent. 1985; 28:760‑5.

Birang R, Poursamimi J, Gutknecht N, Lampert F, Mir M. Comparative evaluation of the effect of Nd:YAG and Er:YAG laser in dentin hypersensitivity treatment. Laser Med Sci. 2007; 22:21‑4.

Abed AM, Mahdian M, Seifi M, Ziaei SA, Shamsaei M. Comparative assessment of the sealing ability of Nd:YAG laser versus a new desensitizing agent in human dentinal tubules: a pilot study. Odontology. 2011; 99(1):45-8.

Fornaini C, Rocca JP, Bertrand MF, Merigo E, Nammour S, Vescovi P. Nd: YAG and diode lasers in the surgical management of soft tissues related to orthodontic treatment. Photomed Laser Surg. 2007; 25:381–92.

Matsumoto K, Kimura Y. Laser Therapy of Dentin Hypersensitivity. J Oral Laser Application. 2007; 7:7-25.

Morioka T, Morita E, Suzuki K. Temperature increments and sensory response of healthy teeth of volunteers with irradiation of a Q-switched Nd-YAG laser. Koku Eisei Gakkai Zasshi. 1982; 31(5):44-50.

Yonaga K, Kimura Y, Matsumoto K. Treatment of cervical dentin hypersensitivity by various methods using pulsed Nd:YAG laser. J Clin Laser Med Surg. 1999; 17(5):205-10.

Fekrazd R, Lotfi G, Gholami GA, Kalhori K. Lasers in Dental Hypersensitivity. JAUMS. 2009; 7(1): 39-45.

Oyama T, Matsumoto K. A clinical and morphological study of cervical hypersensitivity. J Endod .1991; 17(10):500-2.

Brannstrom M. The hydrodynamic theory of dentinalpain: sensation in preparations, caries, and the dentinal crack syndrome. J Endod. 1986; 12: 453-7.

Liu HC, Lin CP, Lan WH. Sealing depth of Nd:YAG laser on human dentinal tubules. J Endod. 1997; 23(11):691-3.

Lan WH, Lui HC. Treatment of dentin hypersensitivity by Nd: YAG Laser. J Clin Laser Med Surg. 1996; 14(2):89-92.

Kimura Y, Wilder-Smith P, Yonaga K , Matsumoto K. Treatment of dentine hypersensitivity by lasers: a review. J Clin Periodontol. 2000; 27(10):715-21.

Farmakis ET, Kozyrakis K, Khabbaz MG, Schoop U, Beer F, Moritz A. In vitro evaluation of dentin tubule occlusion by Denshield and Neodymium-doped yttrium-aluminum-garnet laser irradiation. J Endod. 2012; 38(5):662-6.

Moura-Netto C, Guglielmi Cde A, Mello-Moura AC, Palo RM, Raggio DP, Caldeira CL. Nd:YAG laser irradiation effect on apical intracanal dentin - a microleakage and SEM evaluation. Braz Dent J. 2011; 22(5):377-81.

Al-Saud LM, Al-Nahedh HN. Occluding effect of Nd:YAG laser and different dentin desensitizing agents on human dentinal tubules in vitro: a scanning electron microscopy investigation. Oper Dent. 2012; 37(4):340-55.

Farmakis ET, Beer F, Kozyrakis K, Pantazis N, Moritz A. The influence of different power settings of Nd:YAG laser irradiation, bioglass and combination to the occlusion of dentinal tubules. Photomed Laser Surg. 2013; 31(2):54-8.

Arisu HD, Bala O, Alimzhanova G, Türköz E. Assessment of morphological changes and permeability of apical dentin surfaces induced by Nd:Yag laser irradiation through retrograde cavity surfaces. J Contemp Dent Pract 2004. 15; 5:102-13.

Goya C, Yamazaki R, TomitaY, Kimura Y, Matsumoto K. Effects of pulsed Nd:YAG laser irradiation on smear layer at the apical stop and apical leakage after obturation. Int Endod J. 2000; 33:266-71.

Birang R, Yaghini J, Fakhari E, Hasheminia SM, Mousavi M, Farhadzadeh M, et al. Graphite Application and Different Powers of Nd:YAG Laser on Dentin Surface Changes: A Scanning Electron Microscopy Study. J Lasers Med Sci. 2012; 3(4):153-9.

Birang R, Yaghini J, Shirani AM. Comparative study of dentin surface changes following Nd:YAG and Er:YAG lasers irradiation and implications for hypersensitivity. J Oral Laser Applications. 2008; 8:25-31.

Abstract Viewed: 279 times
PDF Downloaded: 221 times