Effect of Low Level Laser Therapy on Proliferation and Differentiation of the Cells Contributing in Bone Regeneration: A Systematic Review
Journal of Lasers in Medical Sciences,
Vol. 5 No. 4 (2014),
24 September 2014
Introduction: Low level laser therapy (LLLT) also known as photobiomodulation, is a treatment that uses low-level lasers or light-emitting diodes (LEDs) to change cellular function and is a clinically well accepted tool in regenerative medicine and dentistry. Considering the variety of laser, exposure, cells and study types, the exact effects of low level laser therapy seems to be unclear. The aim of this study was to review the data published in the field of the effects of low level laser therapy on proliferation and differentiation of the cells contributing in bone regeneration.
Methods: To access relevant articles, an electronic search in PubMed was conducted from 2001 to April 2014. English language published papers on low level laser therapy were found using the selected keywords .The full texts of potentially suitable articles were obtained for final assessment according to the exclusion and inclusion criteria.
Results: 240 articles were found from 2001 to April 2014. Following the initial screening of titles and abstracts as well as the final screening of full texts, 22 articles completely fulfilled the inclusion criteria of this study. Wavelength used in LLLT irradiation varied between 600 to 1000 nm with an energy density of 0.04–60J/cm2. Although almost all studies agreed on getting positive effects from LLLT, some had opposing results.
Conclusion: Low level laser with low-energy density range appears to exert a biostimulatory effect on bone tissue, enhance osteoblastic proliferation and differentiation on cell lines used in in vitro studies. Despite the fact that many researches have been recently done on the effects of LLLT on different cell lines, without knowing the precise mechanism and effects, we are not able to offer a clinical treatment protocol. This paper is a beginning to help further progress and extend practical use of LLLT in future.
- low-level laser therapies
- cell line
- bone regenerations
How to Cite
Rochkind S, Rousso M, Nissan M, Villarreal M, Barr-Nea L, Rees DG. Systemic effects of low‐power laser irradiation on the peripheral and central nervous system, cutaneous wounds, and burns. Lasers Surg Med 1989;9(2):174-82.
Aggarwal H, Singh MP, Nahar P, Mathur H, Gv S. Efficacy of Low-Level Laser Therapy in Treatment of Recurrent Aphthous Ulcers–A Sham Controlled, Split Mouth Follow Up Study. J Clin Diagn Res 2014;8(2):218-21.
Huertas RM, De Luna-Bertos E, Ramos-Torrecillas J, Leyva FM, Ruiz C, García-Martínez O . Effect and Clinical Implications of the Low-Energy Diode Laser on Bone Cell Proliferation. Biol Res Nurs 2013;16(2):191-6.
Stein A, Benayahu D, Maltz L, Oron U. Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surgery 2005;23(2):161-6.
Renno A, McDonnell P, Crovace M, Zanotto ED, Laakso L. Effect of 830 nm laser phototherapy on osteoblasts grown in vitro on Biosilicate scaffolds. Photomed Laser Surg 2010;28(1):131-3.
Seifi M, Atri F, Yazdani MM. Effects of low-level laser therapy on orthodontic tooth movement and root resorption after artificial socket preservation. Dent Res J 2014;11(1):61-6.
Woodruff L, Bounkeo J, Brannon W, Dawes KS, Barham CD, Waddell DL. The efficacy of laser therapy in wound repair: a meta-analysis of the literature. Photomed Laser Surg 2004;22(3):241-7.
Kim H, Choi K, Kweon O-K, Kim WH. Enhanced wound healing effect of canine adipose-derived mesenchymal stem cells with low-level laser therapy in athymic mice. J Dermatol Sci 2012;68(3):149-56.
Fernandes KR, Ribeiro DA, Rodrigues NC, Tim C, Santos AA, Parizotto NA. Effects of low-level laser therapy on the expression of osteogenic genes related in the initial stages of bone defects in rats. J Biomed Opt 2013;18(3):038002.
Cepera F, Torres F, Scanavini M, Paranhos LR, Capelozza Filho L, Cardoso MA. Effect of a low-level laser on bone regeneration after rapid maxillary expansion. Am J Orthod Dentofacial Orthop 2012;141(4):444-50.
El-Maghraby EM, El-Rouby DH, Saafan AM. Assessment of the effect of low-energy diode laser irradiation on gamma irradiated rats’ mandibles. Arch Oral Biol 2013;58(7):796-805.
Korany NS, Mehanni SS, Hakam HM, El-Maghraby EM. Evaluation of socket healing in irradiated rats after diode laser exposure (histological and morphometric studies). Arch Oral Biol 2012;57(7):884-91.
Migliario M, Pittarella P, Fanuli M, Rizzi M, Renò F. Laser-induced osteoblast proliferation is mediated by ROS production. Lasers Med Sci 2014; 29(4):1463-7.
Bloise N, Ceccarelli G, Minzioni P, Vercellino M1, Benedetti L4, De Angelis MG. Investigation of low-level laser therapy potentiality on proliferation and differentiation of human osteoblast-like cells in the absence/presence of osteogenic factors. J Biomed Optics 2013;18(12):128006.
Bouvet-Gerbettaz S, Merigo E, Rocca JP, Carle GF, Rochet N. Effects of low-level laser therapy on proliferation and differentiation of murine bone marrow cells into osteoblasts and osteoclasts. Lasers Surg Med 2009;41(4):291-7.
Aihara N, Yamaguchi M, Kasai K. Low-energy irradiation stimulates formation of osteoclast-like cells via RANK expression in vitro. Lasers Med Sci 2006;21(1):24-33
Choi K, Kang B, Kim H, Lee S, Bae S, Kweon OK. Low-level laser therapy promotes the osteogenic potential of adipose-derived mesenchymal stem cells seeded on an acellular dermal matrix. J Biomed Mater Res Appl Biomater 2013;101(6):919-28.
Coombe AR, Ho CT, Darendeliler MA, Hunter N, Philips JR, Chapple CC. The effects of low level laser irradiation on osteoblastic cells. Clin Orthod Res 2001;4(1):3-14.
Hamajima S, Hiratsuka K, Kiyama-Kishikawa M, Tagawa T, Kawahara M, Ohta M. Effect of low-level laser irradiation on osteoglycin gene expression in osteoblasts. Lasers Med Sci 2003;18(2):78-82.
Jawad M, Husein A, Azlina A, Alam MK, Hassan R, Shaari R. Effect of 940 nm low-level laser therapy on osteogenesis in vitro. J Biomed Opt 2013;18(12):128001.
Pyo SJ, Song WW, Kim IR, Park BS, Kim CH, Shin SH. Low-level laser therapy induces the expressions of BMP-2, osteocalcin, and TGF-β1 in hypoxic-cultured human osteoblasts. Lasers Med Sci 2013;28(2):543-50.
Stein E, Koehn J, Sutter W, Wendtlandt G, Wanschitz F, Thurnher D. Initial effects of low-level laser therapy on growth and differentiation of human osteoblast-like cells. Wiener klinische Wochenschrift 2008;120(3-4):112-7.
Wu JY, Chen CH, Yeh LY. Low-power laser irradiation promotes the proliferation and osteogenic differentiation of human periodontal ligament cells via cyclic adenosine monophosphate. Int J Oral Sci 2013;5(2):85-91.
Garcia VG, da Conceicao JM, Fernandes LA, de Almeida JM, Nagata MJ, Bosco AF. Effects of LLLT in combination with bisphosphonate on bone healing in critical size defects: a histological and histometric study in rat calvaria. Lasers Med Sci 2013;28(2):407-14.
Aleksic V, Aoki A, Iwasaki K, Takasaki AA, Wang CY, Abiko Y. Low-level Er: YAG laser irradiation enhances osteoblast proliferation through activation of MAPK/ERK. Lasers Med Sci 2010;25(4):559-69.
Renno A, McDonnell P, Parizotto NA, Laakso EL. The effects of laser irradiation on osteoblast and osteosarcoma cell proliferation and differentiation in vitro. Photomed Laser Surg 2007;25(4):275-80.
Martinasso G, Mozzati M, Pol R, Canuto RA, Muzio G. Effect of superpulsed laser irradiation on bone formation in a human osteoblast-like cell line. Minerva stomatol 2007;56(1/2):27.
Mester E, Mester AF, Mester A. The biomedical effects of laser application. Lasers Surg Med 1985;5(1):31-9.
Schultz RJ, Krishnamurthy S, Thelmo W, Rodriguez JE, Harvey G. Effects of varying intensities of laser energy on articular cartilage: a preliminary study. Lasers Surg Med 1985;5(6):577-88.
van Breugel HH, Bar PR. Power density and exposure time of He-Ne laser irradiation are more important than total energy dose in photo-biomodulation of human fibroblasts in vitro. Lasers Surg Med 1992;12(5):528-37.
Anders JJ, Borke RC, Woolery SK, Van de Merwe WP. Low power laser irradiation alters the rate of regeneration of the rat facial nerve. Lasers Surg Med 1993;13(1):72-82.
Balboni GC, Brandi ML, Zonefrati R, Repice F. Effects of He-Ne/I. R. laser irradiation on two lines of normal human fibroblasts in vitro. Arch Ital Anat Embriol 1986;91(3):179-88.
Silva GB, Mendonca EF, Bariani C, Antunes HS, Silva MA. The prevention of induced oral mucositis with low-level laser therapy in bone marrow transplantation patients: a randomized clinical trial. Photomed Laser Surg 2011;29(1):27-31.
Hentschke VS, Jaenisch RB, Schmeing LA, Cavinato PR, Xavier LL, Dal Lago P. Low-level laser therapy improves the inflammatory profile of rats with heart failure. Lasers Med Sci 2013;28(3):1007-16.
Cidral-Filho FJ, Mazzardo-Martins L, Martins DF. Light-emitting diode therapy induces analgesia in a mouse model of postoperative pain through activation of peripheral opioid receptors and the L-arginine/nitric oxide pathway. Lasers Med Sci 2014;29(2):695-702.
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