The Effect of Anterior Stromal Puncture Using Q-Switched Nd:YAG Laser on Corneal Wound Healing
Journal of Lasers in Medical Sciences,
Vol. 5 No. 3 (2014),
22 June 2014
,
Page 121-129
Abstract
Introduction: Recurrent corneal erosion occurs when the wounded corneal epithelium failed to adhere to the underlying stroma. Therefore, this work aimed to assess the effect of treatment of corneal injury using Q- switched Nd:YAG laser.
Method: Twenty one New Zealand male rabbits weighing 2-2.5 kg and 3 months old were classified into three main groups. The control group: did not received any treatment (n=3 rabbits). The rest of the animals (n= 18 rabbits), corneal epithelium was injured by syringe needle and blade 15 and divided into:(A) Normal healing group: which was divided into three subgroups (n=3 rabbits each), and the animals were left for normal healing for1 day, 1 week, and 4 weeks respectively, (B) Laser treated group: divided into three subgroups (n=3 rabbits each) and subjected to anterior stromal puncture using Q-switched Nd: YAG laser on corneal sub-epithelium or superficial stroma, and the animals were left for 1 day, 1 week, and 4 weeks respectively. After the demonstrated periods, the corneas were isolated for estimation of total protein content, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), total antioxidative capacity (TAC), total oxidative capacity (TOC) and oxidative stress index (OSI).
Results: The present results of corneal total protein showed increment in the percentage change in normal healed groups after 1 day, 1 week and 4 weeks by values of 93%, 68% and 39%. In Q-switched Nd: YAG laser treated group the results showed better improvement in corneal protein than normal healed group with percentage changes of 58%, 29%, and 7.5% respectively. In SDS- PAGE, a protein band at 110 KD appeared in the migrating epithelium for both normal healed group and Q-switched Nd:YAG laser treated group with changes in the peaks intensities at middle and low molecular weight regions. Moreover, after 4 weeks the peak at 110 KD disappeared in the wounded epithelium treated with Q-switched Nd:YAG. After four weeks, the OSI in laser treated corneas showed pronounced balance between antioxidative capacity and oxidative capacity.
Conclusion: Anterior stromal puncture by Q-switched Nd:YAG laser is an effective, simple, safe and promising procedure to treat recurrent corneal erosion than normal healing.
- Q-Switched Nd
- YAG lasers
- wound healing
- corneal protein
How to Cite
References
Apple DJ, Robb MF. Cornea. In: Apple DJ, Robb MF, (eds): Ocular pathology vol.2.5th edition. st Louis: Mosby, pp.78-79, 1998.
Letko E, Foster CS. Recurrent erosions syndrome. In: Foster CS et al. (eds), Smolin and Thofts the cornea scientific foundations and clinical practice. 4th edition. Philadelphia: Lippincott William and Wilkins; pp.657-661. 2005.
Reidly JJ, Paulus MP. Recurrent erosions of the cornea: Epidemiology and treatment. Cornea 2000;19(6): 767-71.
Farjo AA, Soong HK. Recurrent corneal erosions. In: Yanoff M , Duk JS (eds). Ophthalmology. Second edition. St Lowis: Mosby, pp. 416-9, 2004.
Gilad E, Baher I, Rolberg B, Weinberger D. Therapeutic contact Lens as primary treatment for traumatic corneal erosions. Isr Med Assoc J 2004; 6(1):28-9.
Sirdhar MS, Rapuano CI, Casar CB, Cohen EJ, Laibson PR. Phototherapeutic Keratectomy versus diamond burr polishing of basement membrane in treatment of RCE associated with anterior basement membrane dystrophy. Ophthalmology 2002; 109 (4):674-9.
Soong HK, Farjo Q, Meyer RF, Sugar A. Diamond burr superficial keratectomy for RCE. Br J Ophthalmol 2002; 86:296-8.
Lin P, Chih-Chiau WU, Lee S. Combined phototherapeutic keratectomy and contact lens for RCES in bullous keratopathy. Br J Ophthalmol 2001; 85:908-11.
O'Brart DP, Muir MG, Marshall J. Phototherapeutic keratectomy for recurrent corneal erosions. Eye 1994; 8:378-83.
Geggel H, Maza C E. Anterior stromal puncture with the Nd:YAG laser. Invest Ophthalmol Vis Sci 1990; 31:1555-9.
Tsai TY, Tsai TH, Hu FR, Hou YC. Recurrent corneal erosions treated with anterior stromal puncture by neodymium: yttrium-aluminum-garnet laser. Ophthalmology 2009; 116(7):1296-300.
Lowry OH, Rosebrough NJ, Farr Al, Randall RJ. Protein measurements with the folin phenol reagent. J Biol Chem 1951; 193:265-75.
Laemmli UK. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 1970; 2777: 680-5.
Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol 2001; 54: 356-61.
Aebi H. Catalase in vitro. Methods Enzymol 1984; 105: 121–6.
Demirbag R, Gur M, Yilmaz R, Kunt AS, Erel O, Andac M H. Influence of oxidative stress on the development of collateral circulation in total coronary occlusions. Int J Cardiol 2007; 116: 14-9.
Snedecore GW, Cochran WG. Statistical Methods. 6th edition, Ames, Iowa University Press, USA, 1976.
Gipson IK, Kiorpes TC. Epithelial sheet movement: protein and glycoprotein synthesis. Dev Biol 1982; 92:259.
Zieske JD, Gipson K. Protein synthesis during corneal epithelial wound healing Invest Ophthalmol Vis Sci 1986; 27:1-7.
Kinoshita S, Friend J, Kiorpes TC, Thoft RA. Keratin-like proteins in corneal and conjunctival epithelium are different. Invest Ophthalmol Vis Sci 1983; 24:577.
Jester JV, Rodrigues MM, Sun TT. Change in keratin expression of corneal epithelium during healing of penetrating corneal wounds. Invest Ophthalmol Vis Sci 1983; 24(Suppl):43.
Gibbins JR. Epithelial migration in organ culture: role of protein synthesis as determined by metabolic inhibitors. Exp Cell Res 1973; 80:281.
Di Pasquale A. Locomotion of epithelial cells: factors involved in extension of the leading edge. Exp Cell Res 1975; 95:425.
Gipson IK, Kiorpes TC, Brennan SJ. Epithelial sheet movement: effects of tunicamycin on migration and glycoprotein synthesis. Dev Biol 1984; 101:212.
Karring H, Thøgersen I B, Klintworth G K, Enghild J J, Møller-Pedersen T. Proteomic analysis of the soluble fraction from human corneal fibroblasts with reference to ocular transparency. Mol Cell Proteomics 2004; 3:660–74.
Wayner DD, Burton GW, Ingold KU, Barclay LR, Locke S J. The relative contributions of vitamin E, urate, ascorbate and proteins to the total peroxyl radical-trapping antioxidant activity of human blood plasma. Biochim Biophys Acta1987; 924: 408-19.
Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 2004; 37:112-9.
Yanik M, Erel O, Kati M. The relationship between potency of oxidative stress and severity of depression. Acta Neuropsychiatr 2004; 16: 200-3.
Harma M, Harma M, Erel O. Oxidative stress in women with preeclampsia. Am J Obstet Gynecol 2005; 192: 656-7.
Yeni E, Gulum M, Selek S, Erel O, Unal D, Verit A, et al. Comparison of oxidative/antioxidative status of penile corpus cavernosum blood and peripheral venous blood. Int J Impot Res 2005; 17:19-22.
Koksal H, Kurban S. Total oxidant status, total antioxidant status, and paraoxonase and arylesterase activities during laparoscopic cholecystectomy. Clinics 2010; 65:285-90.
Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005; 38: 1103-11.
Rabus M, Demirbağ R, Sezen Y, Konukoğlu O, Yildiz A, Erel Ö, et al. Plasma and tissue oxidative stress index in patients with rheumatic and degenerative heart valve disease. Arch Turk Soc Cardiol 2008; 36: 536-40.
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