Comparison of Wavelength-Dependent Penetration Depth of 532 nm and 660 nm Lasers in Different Tissue Types Comparison of wavelength-dependent penetration depth
Journal of Lasers in Medical Sciences,
Vol. 14 (2023),
29 January 2023
,
Page e28
Abstract
Introduction: The depth of laser light penetration into tissue is a critical factor in determining the effectiveness of photodynamic therapy (PDT). However, the optimal laser light penetration depth necessary for achieving maximum therapeutic outcomes in PDT remains unclear. This study aimed to assess the effectiveness of laser light penetration depth at two specific wavelengths, 532 nm and 660 mm.
Methods: Chicken and beef of different thicknesses (1, 3, 5, 10, and 20 mm±0.2 mm) were used as in vitro tissue models. The samples were subjected to irradiation by a low-level laser diode of 532 and 660 nm in continuous mode for 10 minutes. with power densities of 167 and 142 J/cm2, respectively. Laser light transmission through the tissue was measured using a power meter.
Results: For beef samples, the 660 nm wavelength achieved a maximum transmission intensity of 30.7% at 1 cm thickness, while the 532 nm laser had a transmission intensity of 6.5%. Similarly, in chicken breast samples, the maximum transmission occurred at 1 cm thickness with 68.1% for the 660 nm wavelength and 18.2% for the 532 nm laser.
Conclusion: Results consistently demonstrated a significant correlation (P<0.05) between tissue thickness and laser light penetration. Thicker tissues exhibited faster declines in light transmission intensity compared to thinner tissues within 10 minutes. These findings highlight the importance of further research to enhance light delivery in thicker tissues and improve the efficacy of PDT in various medical conditions.
- Photodynamic therapy; Laser light penetration; Tissue thickness; Chicken breast; Optical properties.
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References
Honda N, Ishii K, Terada T, Nanjo T, Awazu K. Determination of the tumor tissue optical properties during and after photodynamic therapy using inverse Monte Carlo method and double integrating sphere between 350 and 1000 nm. J Biomed Opt. 2011;16(5):058003. doi: 10.1117/1.3581111.
Avci P, Gupta A, Sadasivam M, Vecchio D, Pam Z, Pam N, et al. Low-level laser (light) therapy (LLLT) in skin: stimulating, healing, restoring. Semin Cutan Med Surg. 2013;32(1):41-52.
Ash C, Dubec M, Donne K, Bashford T. Effect of wavelength and beam width on penetration in light tissue interaction using computational methods. Lasers Med Sci. 2017;32(8):1909- 18. doi: 10.1007/s10103 017-2317-4.
Tuchin VV. Tissue optics and photonics: light-tissue interaction. J Biomed Photonics Eng. 2015;1(2):98 134.
Mustafa FH, Jaafar MS. Comparison of wavelength-dependent penetration depths of lasers in different types of skin in photodynamic therapy. Indian J Phys. 2013;87(3):203-9. doi: 10.1007/s12648-012-0213-0.
Kaub L, Schmitz C. More than ninety percent of the light energy emitted by near-infrared laser therapy devices used to treat musculoskeletal disorders is absorbed within the first ten millimeters of biological tissue. Biomedicines. 2022;10(12):3204. doi: 10.3390/biomedicines10123204.
Mahmoud BH, Hexsel CL, Hamzavi IH, Lim HW. Effects of visible light on the skin. Photochem Photobiol. 2008;84(2):450-62. doi: 10.1111/j.1751-1097.2007.00286.x.
Mallidi S, Anbil S, Bulin AL, Obaid G, Ichikawa M, Hasan T. Beyond the barriers of light penetration: strategies, perspectives and possibilities for photodynamic therapy. Theranostics. 2016;6(13):2458-87. doi: 10.7150/thno.16183.
Hinz B, Phan SH, Thannickal VJ, Prunotto M, Desmoulière A, Varga J, et al. Recent developments in myofibroblast biology: paradigms for connective tissue remodeling. Am J Pathol. 2012;180(4):1340-55. doi: 10.1016/j.ajpath.2012.02.004.
Sureka CS, Armpilia C. Radiation Biology for Medical Physicists. CRC Press; 2017.
Reece WO, Rowe EW. Functional Anatomy and Physiology of Domestic Animals. John Wiley & Sons; 2017.
Kim MM, Darafsheh A. Light sources and dosimetry techniques for photodynamic therapy. Photochem Photobiol. 2020;96(2):280-94. doi: 10.1111/php.13219.
Jadah N, Shamkhi I. Determination of 650nm wavelength for rapid detection of sickle cell anemia. Res Sq [Preprint]. March 17, 2020. Available from: https://www.researchsquare.com/ article/rs-17409/v1.
Dawood MS. The effects of ultrasound and alternating current on the laser penetration in the tissue. Lasers Med Sci. 2016;31(5):955-64. doi: 10.1007/s10103-016-1937-4.
Mäntele W, Deniz E. UV-VIS absorption spectroscopy: Lambert-Beer reloaded. Spectrochim Acta A Mol Biomol Spectrosc. 2017;173:965-8. doi: 10.1016/j.saa.2016.09.037.
Wang S, Wang J, Yin Q, Wang Y. Light extinction method for solubility measurement. Chin Opt Lett. 2005;3(3):149-51.
Souza-Barros L, Dhaidan G, Maunula M, Solomon V, Gabison S, Lilge L, et al. Skin color and tissue thickness effects on transmittance, reflectance, and skin temperature when using 635 and 808nm lasers in low intensity therapeutics. Lasers Surg Med. 2018;50(4):291-301. doi: 10.1002/lsm.22760.
Baser Keklikci H, Yagci A, Yay AH, Goktepe O. Effects of 405-, 532-, 650-, and 940-nm wavelengths of low-level laser therapies on orthodontic tooth movement in rats. Prog Orthod. 2020;21(1):43. doi: 10.1186/s40510-020-00343-3.
Cios A, Cieplak M, Szymański Ł, Lewicka A, Cierniak S, Stankiewicz W, et al. Effect of different wavelengths of laser irradiation on the skin cells. Int J Mol Sci. 2021;22(5):2437. doi: 10.3390/ijms22052437.
Nasouri B, Murphy TE, Berberoglu H. Near infrared laser penetration and absorption in human skin. In: Mechanisms for Low-Light Therapy IX. Vol 8932. California, United States: SPIE; 2014. p. 67-78. doi: 10.1117/12.2040337.
Nussbaum EL, Van Zuylen J, Jing F. Transmission of light through human skin folds during phototherapy: effects of physical characteristics, irradiation wavelength, and skindiode coupling. Physiother Can. 2007;59(3):194-207. doi:10.3138/ptc.59.3.194.
Kozlovska TI, Kolisnik PF, Zlepko SM, Titova NV, Pavlov VS, Wójcik W, et al. Physical-mathematical model of optical radiation interaction with biological tissues. In: Photonics Applications in Astronomy, Communications, Industry, and High Energy Physics Experiments. Vol 10445. Wilga, Poland: SPIE; 2017. p. 1047-53. doi: 10.1117/12.2280928.
Arslan H, Dolukan YB. Optical penetration depths and fluence distributions in chicken breast and liver tissues. Opt Spectrosc. 2019;127(4):763-8. doi: 10.1134/s0030400x19100035.
Hudson DE, Hudson DO, Wininger JM, Richardson BD. Penetration of laser light at 808and 980nm in bovine tissue samples. Photomed Laser Surg. 2013;31(4):163-8. doi: 10.1089/pho.2012.3284.
Zam A. Laser–tissue interaction. In: Stübinger S, Klämpfl F, Schmidt M, Zeilhofer HF, ed. Lasers in Oral and Maxillofacial Surgery. Cham: Springer; 2020. p. 25-34. doi: 10.1007/978- 3-030-29604-9_3.
Niculescu AG, Grumezescu AM. Photodynamic therapy—an up-to-date review. Appl Sci. 2021;11(8):3626. doi: 10.3390/ app11083626.
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