Investigation into Chronic Low-Dose Ionizing Radiation Effect on Gene Expression Profile of Human HUVECs Cells Chronic Low-Dose Ionizing Radiation Effect on Gene Expression Profile of Human Cell
Journal of Lasers in Medical Sciences,
Vol. 13 (2022),
10 January 2022
,
Page e35
Abstract
Introduction: Understanding the molecular mechanism of chronic low-dose ionizing radiation (LDIR) effects on the human body is the subject of many research studies. Several aspects of cell function such as cell proliferation, apoptosis, inflammation, and tumorigenesis are affected by LDIR. Detection of the main biological process that is targeted by LIDR via network analysis is the main aim of this study. Methods: GSE66720 consisting of gene expression profiles of human umbilical vein endothelial cells (HUVECs) (a suitable cell line to be investigated), including irradiated and control cells, was downloaded from Gene Expression Omnibus (GEO). The significant differentially expressed genes (DEGs) were determined and analyzed via protein-protein interaction (PPI) network analysis to find the central individuals. The main cell function which was related to the central nodes was introduced. Results: Among 64 queried DEGs, 48 genes were recognized by the STRING database. C-X-C motif chemokine ligand 8 (CXCL8), intercellular adhesion molecule 1 (ICAM1), Melanoma growth-stimulatory activity/growth-regulated protein α (CXCL1), vascular cell adhesion molecule 1 (VCAM-1), and nerve growth factor (NGF) were introduced as hub nodes. Conclusion: Findings indicate that inflammation is the main initial target of LDIR at the cellular level which is associated with alteration in the other essential functions of the irradiated cells.
Keywords:
- Radiation, Gene expression, Inflammation, Network analysis, Central node.
How to Cite
Ansari, M. ., Rezaei-Tavirani, M., Hamzeloo-Moghadam, M. ., Razzaghi, M. ., Arjmand, B. ., Zamanian Azodi, M., Khodadoost, M. ., & Okhovatian, F. . (2022). Investigation into Chronic Low-Dose Ionizing Radiation Effect on Gene Expression Profile of Human HUVECs Cells: Chronic Low-Dose Ionizing Radiation Effect on Gene Expression Profile of Human Cell. Journal of Lasers in Medical Sciences, 13, e35. Retrieved from https://journals.sbmu.ac.ir/jlms/article/view/38458
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4. Leszczynski D. Radiation proteomics: a brief overview. Proteomics. 2014;14(4-5):481-8.
5. Bakshi MV, Azimzadeh O, Barjaktarovic Z, Kempf SJ, Merl-Pham J, Hauck SM, et al. Total body exposure to low-dose ionizing radiation induces long-term alterations to the liver proteome of neonatally exposed mice. Journal of proteome research. 2015;14(1):366-73.
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7. Perez-Gelvez YNC, Camus AC, Bridger R, Wells L, Rhodes Jr OE, Bergmann CW. Effects of chronic exposure to low levels of IR on Medaka (Oryzias latipes): a proteomic and bioinformatic approach. International Journal of Radiation Biology. 2021;97(10):1485-501.
8. Bakshi MV, Barjaktarovic Z, Azimzadeh O, Kempf SJ, Merl J, Hauck SM, et al. Long-term effects of acute low-dose ionizing radiation on the neonatal mouse heart: a proteomic study. Radiation and environmental biophysics. 2013;52(4):451-61.
9. Sekaran TSG, Kedilaya VR, Kumari SN, Shetty P, Gollapalli P. Exploring the differentially expressed genes in human lymphocytes upon response to ionizing radiation: a network biology approach. Radiation oncology journal. 2021;39(1):48.
10. Moore R, Puniya BL, Powers R, Guda C, Bayles KW, Berkowitz DB, et al. Integrative network analyses of transcriptomics data reveal potential drug targets for acute radiation syndrome. Scientific reports. 2021;11(1):1-14.
11. Vella D, Marini S, Vitali F, Di Silvestre D, Mauri G, Bellazzi R. MTGO: PPI network analysis via topological and functional module identification. Scientific reports. 2018;8(1):1-13.
12. Athanasios A, Charalampos V, Vasileios T. Protein-protein interaction (PPI) network: recent advances in drug discovery. Current drug metabolism. 2017;18(1):5-10.
13. Yu D, Li Y, Ming Z, Wang H, Dong Z, Qiu L, et al. Comprehensive circular RNA expression profile in radiation-treated HeLa cells and analysis of radioresistance-related circRNAs. PeerJ. 2018;6:e5011.
14. Rezaei Tavirani M, Arjmand B, Razzaghi M, Ahmadzadeh A. 50S Ribosomal proteins family is the main target of cinnamon extract: a network analysis. Research Journal of Pharmacognosy. 2021;8(2):63-8.
15. Rasoulpour T, DiPalma K, Kolvek B, Hixon M. Akt1 suppresses radiation-induced germ cell apoptosis in vivo. Endocrinology. 2006;147(9):4213-21.
16. Oeck S, Al-Refae K, Riffkin H, Wiel G, Handrick R, Klein D, et al. Activating Akt1 mutations alter DNA double strand break repair and radiosensitivity. Scientific reports. 2017;7(1):1-11.
17. Saligan L, Hsiao C, Wang D, Wang X, John LS, Kaushal A, et al. Upregulation of α-synuclein during localized radiation therapy signals the association of cancer-related fatigue with the activation of inflammatory and neuroprotective pathways. Brain, behavior, and immunity. 2013;27:63-70.
18. Brach M, Gruss H, Kaisho T, Asano Y, Hirano T, Herrmann F. Ionizing radiation induces expression of interleukin 6 by human fibroblasts involving activation of nuclear factor-kappa B. Journal of Biological Chemistry. 1993;268(12):8466-72.
19. Pal S, Yadav P, Sainis K, Shankar BS. TNF-α and IGF-1 differentially modulate ionizing radiation responses of lung cancer cell lines. Cytokine. 2018;101:89-98.
20. Gales D, Clark C, Manne U, Samuel T. The chemokine CXCL8 in carcinogenesis and drug response. International Scholarly Research Notices. 2013;2013.
21. Singh M, Thakur M, Mishra M, Yadav M, Vibhuti R, Menon AM, et al. Gene regulation of intracellular adhesion molecule-1 (ICAM-1): A molecule with multiple functions. Immunology Letters. 2021;240:123-36.
22. Dhawan P, Richmond A. Role of CXCL1 in tumorigenesis of melanoma. Journal of leukocyte biology. 2002;72(1):9-18.
23. Jiang L, Yang A, Li X, Liu K, Tan J. Down-regulation of VCAM-1 in bone mesenchymal stem cells reduces inflammatory responses and apoptosis to improve cardiac function in rat with myocardial infarction. International Immunopharmacology. 2021;101:108180.
24. Ceci FM, Ferraguti G, Petrella C, Greco A, Tirassa P, Iannitelli A, et al. Nerve growth factor, stress and diseases. Current Medicinal Chemistry. 2021;28(15):2943-59.
25. Loseva O, Shubbar E, Haghdoost S, Evers B, Helleday T, Harms-Ringdahl M. Chronic low dose rate ionizing radiation exposure induces premature senescence in human fibroblasts that correlates with up regulation of proteins involved in protection against oxidative stress. Proteomes. 2014;2(3):341-62.
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