The Effect of Low-Level Laser Therapy on the Viability of Human Dental Pulp Stem Cells Effect of LLL on the viability of DPSCs
Journal of Lasers in Medical Sciences,
Vol. 13 (2022),
10 January 2022
,
Page e60
Abstract
Objectives: This study assessed the effect of low-level laser (LLL) irradiation on the viability of dental pulp stem cells (DPSCs).
Materials and Methods: In this in vitro experimental study, human DPSCs were purchased from the cell bank of Iranian Genetic Resources and cultured in flasks containing Dulbecco's modified Eagle's medium supplemented with 20% fetal bovine serum (FBS) at 37°C, 5% CO2, and 95% humidity. The cells were stored in semi-confluent form, and the culture medium was refreshed every two days. The cells in the control group were not laser-irradiated. The cells in the experimental groups were irradiated with 660 and 808 nm diode lasers with 4.1 J/cm2 energy density. Cell viability was assessed at baseline and after 24, 48, and 72 hours using the methyl thiazolyl tetrazolium (MTT) assay. The effects of laser irradiation, laser wavelength, and time on the percentage of cell viability were analyzed by two-way ANOVA and Tukey's test.
Results: The effects of laser irradiation and its wavelength (P=0.04), time of assessment (P<0.001), and the interaction effect of group and time (P=0.02) on cell viability were significant. Cell viability in 660 and 808 nm laser groups at 48 and 72 hours was higher than that of the control group; however, statistically, only the difference in cell viability between the 660 nm laser and control group at 72 hours was significant (P=0.03).
Conclusion: Considering the optimal effect of diode laser irradiation (particularly 660 nm) on the viability of DPSCs, it may be suitable for relevant clinical applications.
- Cell survival; Low-level light therapy; Dental pulp; Stem cells
How to Cite
References
2. Hübler R, Blando E, Gaião L, Kreisner PE, Post LK, Xavier CB, de Oliveira MG. Effects of low-level laser therapy on bone formed after distraction osteogenesis. Lasers in medical science. 2010 Mar;25(2):213-9.
3. Convissar RA, Tunér J, Beck-Kristensen PH, Ross G, Ross A. Photo-biomodulation in dentistry. Principles and Practice of Laser Dentistry 2nd Edition. 2016:251–74.
4. Chung H, Dai T, Sharma SK, Huang YY, Carroll JD, Hamblin MR. The nuts and bolts of low-level laser (light) therapy. Annals of biomedical engineering. 2012 Feb;40(2):516-33.
5. Karu T. Mitochondrial Mechanisms of Photobiomodulation in Context of New Data About Multiple Roles of ATP. Photomedicine and laser surgery. 2010 Apr;28(2):159-60.
6. Kushibiki T, Hirasawa T, Okawa S, Ishihara M. Regulation of miRNA expression by low-level laser therapy (LLLT) and photodynamic therapy (PDT). International journal of molecular sciences. 2013 Jul;14(7):13542-58.
7. Montoro LA, Turrioni AP, Basso FG, de Souza Costa CA, Hebling J. Infrared LED irradiation photobiomodulation of oxidative stress in human dental pulp cells. International endodontic journal. 2014 Aug;47(8):747-55.
8. Barolet D. Light-emitting Diodes (LEDs) in Dermatology. Seminars in cutaneous medicine and surgery 2008 Dec (Vol. 27, No. 4, pp. 227-238).
9. Ginani F, Soares DM, Barboza CA. Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review. Lasers in medical science. 2015 Nov;30(8):2189-94.
10. Jabari F, Mohammadnejad J, Yavari K. Human primary dental pulp mesenchymal stem cells: History and isolation methods. Journal of Dental Medicine. 2014 Oct 10;27(3):184-9.
11. Hayashi Y, Imai M, Goto Y, Murakami N. Pathological Mineralization in a Serially Passaged Cell Line From Rat Pulp. Journal of oral pathology & medicine: official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. 1993 Apr;22(4):175-9.
12. Nagata T, Yokota M, Ohishi K, Nishikawa S, Shinohara H, Wakano Y, Ishida H. 1α, 25-dihydroxy vitamin D3 stimulation of osteopontin expression in rat clonal dental pulp cells. Archives of oral biology. 1994 Sep 1;39(9):775-82.
13. Kamata N, Fujimoto R, Tomonari M, Taki M, Nagayama M, Yasumoto S. Immortalization of human dental papilla, dental pulp, periodontal ligament cells and gingival fibroblasts by telomerase reverse transcriptase. Journal of oral pathology & medicine. 2004 Aug;33(7):417-23.
14. Galler KM, Schweikl H, Thonemann B, D'Souza RN, Schmalz G. Human Pulp-Derived Cells Immortalized With Simian Virus 40 T-antigen. European journal of oral sciences. 2006 Apr;114(2):138-46.
15. Xinaris C, Morigi M, Benedetti V, Imberti B, Fabricio AS, Squarcina E, Benigni A, Gagliardini E, Remuzzi G. A novel strategy to enhance mesenchymal stem cell migration capacity and promote tissue repair in an injury specific fashion. Cell transplantation. 2013 Mar;22(3):423-36.
16. Mimeault M, Batra SK. Aging of tissue-resident adult stem/progenitor cells and their pathological consequences. Panminerva medica. 2009 Jun 1;51(2):57-79.
17. Wagner W, Bork S, Horn P, Krunic D, Wallenda T, Diehlmann A, Benes V, Blake J, Huber FX, Eckstein V, Boukamp P. Aging and Replicative Senescence Have Related Effects on Human Stem and Progenitor Cells. PloS one. 2009 Jun 9;4(6):e5846.
18. Li WT, Chen CW, Huang PY. Effects of Low-Level Light Irradiation on the Migration of Mesenchymal Stem Cells Derived From Rat Bone Marrow. Conference proceedings:... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference 2013 (Vol. 2013, pp. 4121-4124).
19. Kim H, Choi K, Kweon OK, Kim WH. Enhanced wound healing effect of canine adipose-derived mesenchymal stem cells with low-level laser therapy in athymic mice. Journal of dermatological science. 2012 Dec 1;68(3):149-56.
20. Shingyochi Y, Kanazawa S, Tajima S, Tanaka R, Mizuno H, Tobita M. A Low-Level Carbon Dioxide Laser Promotes Fibroblast Proliferation and Migration Through Activation of Akt, ERK, and JNK. PloS one. 2017 Jan 3;12(1):e0168937.
21. Karu T. Primary and Secondary Mechanisms of Action of Visible to near-IR Radiation on Cells. Journal of photochemistry and photobiology. B, Biology. 1999 Mar;49(1):1-17.
22. Pogrel MA, Chen JW, Zhang K. Effects of low‐energy gallium‐aluminum‐arsenide laser irradiation on cultured fibroblasts and keratinocytes. Lasers in Surgery and Medicine: The Official Journal of the American Society for Laser Medicine and Surgery. 1997;20(4):426-32.
23. Reddy GK, Stehno-Bittel L, Enwemeka CS. Laser Photostimulation of Collagen Production in Healing Rabbit Achilles Tendons. Lasers in surgery and medicine. 1998;22(5):281-7.
24. Takasaki AA, Aoki A, Mizutani K, Schwarz F, Sculean A, Wang C, Geena K, Romanos G, Ishikawa I, Izumi Y. Application of antimicrobial photodynamic therapy in periodontal and peri-implant diseases. Periodontology 2000. 2009;51(1):109-40.
25. Paschalidou M, Athanasiadou E, Arapostathis K, Kotsanos N, Koidis PT, Bakopoulou A, Theocharidou A. Biological effects of low-level laser irradiation (LLLI) on stem cells from human exfoliated deciduous teeth (SHED). Clinical oral investigations. 2020 Jan;24(1):167-80.
26. Ferreira LS, Diniz IM, Maranduba CM, Miyagi SP, Rodrigues MF, Moura-Netto C, Marques MM. Short-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cells. Lasers in medical science. 2019 Jun;34(4):659-66.
27. Almeida-Lopes L, Rigau J, Zângaro RA, Guidugli-Neto J, Jaeger MM. Comparison of the low-level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence. Lasers in surgery and medicine. 2001;29(2):179-84.
28. Pereira AN, Eduardo CD, Matson E, Marques MM. Effect of low‐power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts. Lasers in Surgery and Medicine: The Official Journal of the American Society for Laser Medicine and Surgery. 2002 Oct;31(4):263-7.
29. Azevedo LH, de Paula Eduardo F, Moreira MS, de Paula Eduardo C, Marques MM. Influence of Different Power Densities of LILT on Cultured Human Fibroblast Growth: A Pilot Study. Lasers in medical science. 2006 Jul;21(2):86-9.
30. Eduardo FD, Bueno DF, de Freitas PM, Marques MM, Passos‐Bueno MR, Eduardo CD, Zatz M. Stem cell proliferation under low intensity laser irradiation: a preliminary study. Lasers in Surgery and Medicine: The Official Journal of the American Society for Laser Medicine and Surgery. 2008 Aug;40(6):433-8.
31. Moura-Netto C, Ferreira LS, Maranduba CM, Mello-Moura AC, Marques MM. Low-intensity Laser Phototherapy Enhances the Proliferation of Dental Pulp Stem Cells Under Nutritional Deficiency. Brazilian oral research. 2016 May 31;30(1): S1806-83242016000100265.
32. Ganjali M, Seifalian AM, Mozafari M. Effect of Laser Irradiation on Cell Cycle and Mitosis. Journal of lasers in medical sciences. 2018;9(4):249-53.
33. Borzabadi-Farahani A. Effect of Low-Level Laser Irradiation on Proliferation of Human Dental Mesenchymal Stem Cells; A Systemic Review. Journal of photochemistry and photobiology. B, Biology. 2016 Sep;162:577-82.
34. Zaccara IM, Ginani F, Mota-Filho HG, Henriques ÁC, Barboza CA. Effect of low-level laser irradiation on proliferation and viability of human dental pulp stem cells. Lasers in medical science. 2015 Dec;30(9):2259-64.
35. AlGhamdi KM, Kumar A, Moussa NA. Low-level Laser Therapy: A Useful Technique for Enhancing the Proliferation of Various Cultured Cells. Lasers in medical science. 2012 Jan;27(1):237-49.
36. Koutná M, Janisch R, Veselska R. Effects of low-power laser irradiation on cell proliferation. Scripta Medica. 2003 Jun;76(3):163-72.
- Abstract Viewed: 268 times
- PDF Downloaded: 226 times