Comparison of Manual Tools, Ultrasonic and Erbium-Doped Yttrium Aluminum Garnet (Er:YAG) Laser on the Debridement Effect of the Surface of the Root of Teeth Suffering from Periodontitis
Journal of Lasers in Medical Sciences,
Vol. 4 No. 4 (2013),
26 Mehr 2013
,
Page 199-205
Abstract
Introduction: Periodontal diseases are considered as some of the most common reasons of teeth loss, which occur due to the aggregation of microbial plaque and other precipitations on the dental surfaces. In this study, the scaling effect using manual tools, ultrasonic machine and Erbium-Doped Yttrium Aluminum Garnet (Er:YAG)laser on the connection of the human gums connective tissue cells on the root surface of the teeth suffering from severe periodontitis will be compared.
Methods: After removal of the big precipitations with manual tools, Er:YAG laser light emission of Photona machine is used with respect to the following characteristics: wavelength: 2940μm, each pulse: 100mJ, frequency: 10 pulse/sec, optic fiber with cross section 0.5x1.65mm, fiber tip angle with root surface: 15-20 degrees with non-contact mode, 1.5mm farther than the root surface and pulse duration 230 very short. The gingival fibroblast cellular was incubated as a sample of the human gums connective tissue cells under 37C. These cells were departed from the culture medium after the cellular reproduction in the third passage.On the 3rd day after incubation, the gingival fibroblast cells morphology was studied by Scanning Electron Microscopy (SEM).
Results: The results of SEM images in the present study indicated the spread fibroblast cells with philopodia were found in all of 5 groups; untreated healthy group (control), untreated group suffering from periodontitis, the scaling effect using manual tools (Scaled Gracey), ultrasonic machine and Er:YAG laser. There is a meaningful difference among the three treatment groups (P<0.001) in the numbers of the fibroblast cells, while all the four treated groups had a meaningful difference with the positive control group (P < 0.001).
Conclusion: The present study indicated that although various dental surfaces cleaning methods may be different in other aspects, but are similar concerning the fibroblasts morphology. Also in addition to power, laser emission time may also be effective in the cells morphology results.
- Er-YAG laser
- periodontitis
- rate
- survival
How to Cite
References
Newman MG, Takei HH, Carranza FA. Carranza’s clinical periodontology. 11th ed. Philadelphia: WB Saunders Co. 2011.
Drisko CL. Scaling and root planning without overinstrumentation: hand versus power-driven scalers. Curr Opin Periodontol 1993;3:78-88.
Ashimoto A, Chen C, Bakker J, Slots J. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbial Immunol 1996;11(4):266-73.
Amid R, Movahedi M. Application of laser in clinical dentistry. 1st edition, Tehran, Shayan Nemoudar Press, 2006.
Frentzen M, Koort HJ. Lasers in dentistry: new possibilities with advancing laser technology. Int Dent J 1990;40(6):323-32.
Maruyama H, Aoki A, Sasaki KM, Takasaki AA, Iwasaki K, Ichinose SH, et al. The effect of chemical and/or mechanical conditioning on the Er:YAG laser-treated root cementum: analysis of surface morphology and periodontal ligament fibroblast attachment. Laser Surg Med 2008; 40(3):211-22.
Belal MH, Watanabe H, Ichinose S, Ishikawa I. Effect of Er:YAG laser combined with rhPDGF-BB on attachment of cultured firbroblasts to periodontally involved root surfaces. J Periodontol 2007 ;78:1329-41.
Schwarz F, Aoki A, Sculean A, George T, Scherbaum W, Becker J. In vivo effects of an Er:YAG laser, an ultrasonic system and scaling and root planning on the biocompatibility of periodontally diseased and surfaces in cultures of human PDL fibroblasts. Lasers Surg Med 2003;33(2):140-7.
Mizutani K, Aoki A, Takasaki AA, Kinshita A, Hayashi C, Oda SH, et al. Periodontal tissue healing following flap surgery using an Er:YAG laser in dogs. Lasers Surg Med 2006;38(4):314-24.
-Romanos G, Crespi R, Barone A, Covani U. Osteoblast attachment on titanium disks after laser irradiation. Int J Oral Maxillofac Implants 2006;21(3):232-6.
Hakki SS, Korkusuz P, Berk G, Dundar N, Saglam M, Bozkurt B, et al. Comparison of Er,Cr: YGG Laser and Hand Instrumentation on the Attachment of periodontal Ligament Fibroblasts to Periodontally Diseased Root Surfaces: An In Vitro Study. J Periodontol 2010 ;81(8):1216-25.
Stubinger S, Etter C, Miskiewick M, Homann F, Saldamli B, Wieland M, Sader R. Surface alteration of polished and sandblasted and acid-etched titanium implants after Er:YAG, Carbone Dioxide, And Diode laser irradiation. Int J Oral Maxillofac Implants. 2010;25:104-11
- Abstract Viewed: 270 times
- PDF Downloaded: 155 times