Neuroprotective Properties of Photobiomodulation in Retinal Regeneration in Rats: Perspectives From Interaction Levels
Journal of Lasers in Medical Sciences,
Vol. 11 No. 3 (2020),
21 June 2020
,
Page 280-286
Abstract
Introduction: Photobiomodulation (PBM) is known as low-level laser (or light) therapy and is applied in different fields of medicine. However, it is required that its molecular and cellular mechanism be investigated. This study aims to assess the neuroprotective properties of PBM in the rat retina.
Methods: GSE22818 was downloaded from Gene Expression Omnibus (GEO) and the regulation of the significant differentially expressed genes (DEGs) which are produced by light damage in the rat retina by the pretreatment of PBM application was assessed via network analysis and gene ontology enrichment.
Results: The 78 produced DEGs by light-damage in the rat retina were protected via PBM pretreatment action. Among these determined DEGs, 53 individuals were included in the main connected component of the constructed protein-protein interaction (PPI) network. Ccl2, Icam1, Cxcl10, Timp1, and Fos were determined as hub nodes. Eight clusters including 26 regulated biochemical pathways by PBM pretreatment were identified. The critical DEGs based on the action maps were introduced.
Conclusion: The finding indicates that PBM treatment protects rat retina against light damage via the prevention of Fos, Ccl2, Icam1, Cxcl10, and Myc dysregulation.
- Photobiomodulation therapy
- Retina
- Gene expression
- Protein-protein interaction network
- Rat.
How to Cite
References
Hamblin MR. Mechanisms and applications of the anti-inflammatory effects of photobiomodulation. AIMS biophys. 2017;4(3):337-61. doi: 10.3934/biophy.2017.3.337.
de Freitas LF, Hamblin MR. Proposed mechanisms of photobiomodulation or low-level light therapy. IEEE J Sel Top Quantum Electron. 2016;22(3):7000417. doi: 10.1109/JSTQE.2016.2561201.
Geneva II. Photobiomodulation for the treatment of retinal diseases: a review. Int J Ophthalmol. 2016;9(1):145-52. doi: 10.18240/ijo.2016.01.24.
Eells JT. Photobiomodulation in animal models of retinal injury and disease. In: Hamblin MR, Huang YY, editors. Photobiomodulation in the Brain: Low-level laser (light) therapy in neurology and neuroscience. San Diego: Academic Press; 2019. P. 265-73. doi: 10.1016/B978-0-12-815305-5.00021-X.
Eells JT, Gopalakrishnan S, Valter K. Near-infrared photobiomodulation in retinal injury and disease. In: Bowes Rickman C, LaVail MM, Anderson RE, Grimm C, Hollyfield J, Ash J, editors. Retinal Degenerative Diseases: Mechanisms and Experimental Therapy. New York:
Springer; 2016. p. 437-41. doi: 10.1007/978-3-319-17121-0_58.
Eells JT, Henry MM, Summerfelt P, Wong-Riley MT, Buchmann EV, Kane M, et al. Therapeutic photobiomodulation for methanol-induced retinal toxicity. Proc Natl Acad Sci U S A. 2003;100(6):3439-44. doi: 10.1073/pnas.0534746100.
Eells JT, DeSmet KD, Kirk DK, Wong-Riley M, Whelan HT, Ver Hoeve J, et al. Photobiomodulation for the treatment of retinal injury and retinal degenerative diseases. In: Waynant R, Tata DB, editors. Proceedings of light-activated tissue regeneration and therapy conference. New York: Springer; 2008. doi: 10.1007/978-0-387-71809-5
Natoli R, Zhu Y, Valter K, Bisti S, Eells J, Stone J. Gene and noncoding RNA regulation underlying photoreceptor protection: microarray study of dietary antioxidant saffron and photobiomodulation in rat retina. Mol Vis. 2010;16:1801-22.
Razzaghi MR, Rostami-Nejad M, Rezaei-Tavirani M, Zamanian Azodi M, Okhovatian F, Mansouri V, et al. Muscle recovery is highlighted by IR laser therapy. J Lasers Med Sci. 2019;10(Suppl 1):S49-S53. doi: 10.15171/jlms.2019.S9.
Rostami-Nejad M, Rezaei-Tavirani M, Zadeh-Esmaeel MM, Rezaei Tavirani S, Akbari Z, Esmaeili S, et al. Assessment of cytokine-mediated signaling pathway dysregulation in arm skin after CO2 laser therapy. J Lasers Med Sci. 2019;10(4):257-63. doi: 10.15171/jlms.2019.42.
Mansouri V, Rezaei-Tavirani M, Zadeh-Esmaeel MM, Rezaei-Tavirani S, Razzaghi M, Okhovatian F, et al. Analysis of laser therapy effects on squamous cell carcinoma patients: A system biology study. J Lasers Med Sci. 2019;10(Suppl 1):S1-S6. doi: 10.15171/jlms.2019.S1.
Shahrokh S, Razzaghi Z, Mansouri V, Ahmadi N. Impact of proteomics investigation on the development and improvement of skin laser therapy: A review article. J Lasers Med Sci. 2019;10(Suppl 1):S90-S95. doi: 10.15171/jlms.2019.S16
Asadzadeh-Aghdaei H, Zadeh-Esmaeel MM, Esmaeili S, Rezaei-Tavirani M, Rezaei-Tavirani S, Mansouri V, et al. Effects of high fat medium condition on cellular gene expression profile: a network analysis approach. Gastroenterol Hepatol Bed Bench. 2019;12(Suppl 1):S130-S135.
Mlecnik B, Galon J, Bindea G. Automated exploration of gene ontology term and pathway networks with ClueGO-REST. Bioinformatics. 2019;35(19):3864-6. doi: 10.1093/bioinformatics/btz163.
Bindea G, Galon J, Mlecnik B. CluePedia Cytoscape plugin: pathway insights using integrated experimental and in silico data. Bioinformatics. 2013;29(5):661-3. doi: 10.1093/bioinformatics/btt019.
Wong-Riley MT, Liang HL, Eells JT, Chance B, Henry MM, Buchmann E, et al. Photobiomodulation directly benefits primary neurons functionally inactivated by toxins: role of cytochrome c oxidase. J Biol Chem. 2005;280(6):4761-71. doi: 10.1074/jbc.m409650200.
Hamblin MR, Nelson ST, Strahan JR. Photobiomodulation and cancer: what is the truth? Photomed Laser Surg. 2018;36(5):241-5. doi: 10.1089/pho.2017.4401.
Dalla-Favera R, Martinotti S, Gallo RC, Erikson J, Croce CM. Translocation and rearrangements of the c-myc oncogene locus in human undifferentiated B-cell lymphomas. Science. 1983;219(4587):963-7. doi: 10.1126/science.6401867.
Boxer LM, Dang CV. Translocations involving c-myc and c-myc function. Oncogene. 2001;20(40):5595-610. doi: 10.1038/sj.onc.1204595.
Becker A, Klapczynski A, Kuch N, Arpino F, Simon-Keller K, De La Torre C, et al. Gene expression profiling reveals aryl hydrocarbon receptor as a possible target for photobiomodulation when using blue light. Sci Rep. 2016;6:33847. doi: 10.1038/srep33847.
Pansani TN, Basso FG, Soares DG, Turrioni APDS, Hebling J, de Souza Costa CA. Photobiomodulation in the metabolism of lipopolysaccharides‐exposed epithelial cells and gingival fibroblasts. Photochem Photobiol. 2018;94(3):598-603. doi: 10.1111/php.12877.
- Abstract Viewed: 640 times
- PDF Downloaded: 483 times