Photobiomodulation Therapy in the Proliferation and Differentiation of Human Umbilical Cord Mesenchymal Stem Cells: An In Vitro Study
Journal of Lasers in Medical Sciences,
Vol. 11 No. 4 (2020),
3 October 2020
,
Page 469-474
Abstract
Introduction: Since photobiomodulation therapy (PBMT) favors in vitro mesenchymal stem cell (MSC) preconditioning before MSC transplantation, increasing the proliferation of these cells without molecular injuries by conserving their characteristics, in the present in vitro study we analyzed the effect of PBMT on the proliferation and osteogenic differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs).
Methods: Irradiation with an InGaAIP Laser (660 nm, 10 mW, 2.5 J/cm2, 0.08 cm2 spot size, and 10 s) was carried out. The cells were divided into four groups: CONTROL [cells grown in Dulbecco’s Modified Eagle Medium (DMEM)], OSTEO (cells grown in an osteogenic medium); PBMT (cells grown in DMEM+PBMT), and OSTEO+PBMT (cells grown in an osteogenic medium-plus PBMT). The cell proliferation curve was obtained over periods of 24, 48 and 72 hours using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Osteogenic differentiation was analyzed by the formation of calcium nodules over periods of 7, 14 and 21 days. Morphometric analysis was performed to quantify the total area of nodular calcification.
Results: The highest cell proliferation and cell differentiation occurred in the OSTEO+PBMT group, followed by the PBMT, OSTEO, and CONTROL groups respectively, at the observed times (P < 0.05).
Conclusion: PBMT enhanced the osteogenic proliferation and the differentiation of hUCMSCs during the periods tested, without causing damage to the cells and preserving their specific characteristics, a fact that may represent an innovative pretreatment in the application of stem cells.
- Photobiomodulation
- Lasers
- Stem cells
- Cell differentiation
How to Cite
References
de Sá Silva F, Almeida PN, Rettore JV, Maranduba CP, de Souza CM, de Souza GT, Zanette Rde S, Miyagi SP, Santos Mde O, Marques MM, Maranduba CM. Toward personalized cell therapies by using stem cells: seven relevant topics for safety and success in stem cell therapy. J Biomed Biotechnol. 2012;2012:758102. doi.org/10.1155/2012/758102.
De Los Angeles A, Ferrari F, Xi R, Fujiwara Y, Benvenisty N, Deng H, Hochedlinger K, Jaenisch R, Lee S, Leitch HG, Lensch MW, Lujan E, Pei D, Rossant J, Wernig M, Park PJ, Daley GQ. Hallmarks of pluripotency. Nature. 2015;525:469-478. doi.org/10.1038/nature15515.
Otsu K, Kumakami-Sakano M, Fujiwara N, Kikuchi K, Keller L, Lesot H, Harada H. Stem cell sources for tooth regeneration: current status and future prospects. Front Physiol. 2014;5:36. 10.3389/fphys.2014.00036.
Ding DC, Chang YH, Shyu WC, Lin SZ. Human umbilical cord mesenchymal stem cells: a new era for stem cell therapy. Cell Transplant. 2015;24:339-347. doi.org/10.3727/096368915X686841.
Hassan G, Bahjat M, Kasem I, Soukkarieh C, Aljamali M. Platelet lysate induces chondrogenic differentiation of umbilical cord-derived mesenchymal stem cells. Cell Mol Biol Lett. 2018;23:11. doi.org/10.1186/s11658-018-0080-6.
Naderi H, Matin MM, Bahrami AR. Review paper: critical issues in tissue engineering: biomaterials, cell sources, angiogenesis, and drug delivery systems. J Biomater Appl. 2011;26:383-417. doi.org/10.1177/0885328211408946.
Fekrazad R, Asefi S, Allahdadi M, Kalhori KA. Effect of Photobiomodulation on Mesenchymal Stem Cells. Photomed Laser Surg. 2016;34:533-542. doi.org/10.1089/pho.2015.4029.
Çakmak AS, Çakmak S, Vatansever HS, Gümüşderelioğlu M. Photostimulation of osteogenic differentiation on silk scaffolds by plasma arc light source. Lasers Med Sci. 2018;33:785-794. doi.org/10.1007/s10103-017-2414-4.
Soares DM, Ginani F, Henriques ÁG, Barboza CA. Effects of laser therapy on the proliferation of human periodontal ligament stem cells. Lasers Med Sci. 2015;30:1171-1174. doi.org/10.1007/s10103-013-1436-9.
Yin K, Zhu R, Wang S, Zhao RC. Low-Level Laser Effect on Proliferation, Migration, and Antiapoptosis of Mesenchymal Stem Cells. Stem Cells Dev. 2017;26:762-775. doi.org/10.1089/scd.2016.0332.
Engel KW, Khan I, Arany PR. Cell lineage responses to photobiomodulation therapy. J Biophotonics. 2016;9:1148-1156. doi.org/10.1002/jbio.201600025.
de Freitas LF, Hamblin MR. Proposed Mechanisms of Photobiomodulation or Low-Level Light Therapy. IEEE J Sel Top Quantum Electron. 2016;22:7000417. doi.org/10.1109/JSTQE.2016.2561201.
Mvula B, Abrahamse H. Differentiation Potential of Adipose-Derived Stem Cells When Cocultured with Smooth Muscle Cells, and the Role of Low-Intensity Laser Irradiation. Photomed Laser Surg. 2016;34:509-515. doi.org/10.1089/pho.2015.3978.
Li Y, He L, Pan S, Zhang L, Zhang W, Yi H, Niu Y. Three-dimensional simulated microgravity culture improves the proliferation and odontogenic differentiation of dental pulp stem cell in PLGA scaffolds implanted in mice. Mol Med Rep. 2017;15:873-878. doi.org/10.3892/mmr.2016.6042.
Zaccara IM, Ginani F, Mota-Filho HG, Henriques ÁC, Barboza CA. Effect of low-level laser irradiation on proliferation and viability of human dental pulp stem cells. Lasers Med Sci. 2015;30:2259-2264. doi.org/10.1007/s10103-015-1803-9.
Humbert P, Brennan MÁ, Davison N, Rosset P, Trichet V, Blanchard F, Layrolle P. Immune Modulation by Transplanted Calcium Phosphate Biomaterials and Human Mesenchymal Stromal Cells in Bone Regeneration. Front Immunol 2019;10:663. doi.org/10.3389/fimmu.2019.00663.
Wang L, Wu F, Liu C, Song Y, Guo J, Yang Y, Qiu Y. Low-level laser irradiation modulates the proliferation and the osteogenic differentiation of bone marrow mesenchymal stem cells under healthy and inflammatory condition. Lasers Med Sci. 2019;34:169-178. doi.org/10.1007/s10103-018-2673-8.
Park IS, Mondal A, Chung PS, Ahn JC. Vascular regeneration effect of adipose-derived stem cells with light-emitting diode phototherapy in ischemic tissue. Lasers Med Sci. 2015;30:533-541. doi.org/10.1007/s10103-014-1699-9.
Blatt A, Elbaz-Greener GA, Tuby H, Maltz L, Siman-Tov Y, Ben-Aharon G, Copel L, Eisenberg I, Efrati S, Jonas M, Vered Z, Tal S, Goitein O, Oron U. Low-Level Laser Therapy to the Bone Marrow Reduces Scarring and Improves Heart Function Post-Acute Myocardial Infarction in the Pig. Photomed Laser Surg. 2016;34:516-524. doi.org/10.1089/pho.2015.3988.
Bayat M, Virdi A, Rezaei F, Chien S. Comparison of the in vitro effects of low-level laser therapy and low-intensity pulsed ultrasound therapy on bony cells and stem cells. Prog Biophys Mol Biol. 2018;133:36-48. doi.org/10.1016/j.pbiomolbio.2017.11.001.
Peplow PV, Chung TY, Ryan B, Baxter GD. Laser photobiomodulation of gene expression and release of growth factors and cytokines from cells in culture: a review of human and animal studies. Photomed Laser Surg. 2011;29:285-304. doi.org/10.1089/pho.2010.2846.
Zajdel A, Kałucka M, Kokoszka-Mikołaj E, Wilczok A. Osteogenic differentiation of human mesenchymal stem cells from adipose tissue and Wharton's jelly of the umbilical cord. Acta Biochim Pol. 2017;64:365-369. doi.org/10.18388/abp.2016_1488.
Hamblin MR. Mechanisms and Mitochondrial Redox Signaling in Photobiomodulation. Photochem Photobiol. 2018;94:199-212. doi.org/10.1111/php.12864.
Pinheiro AL, Gerbi ME. Photoengineering of bone repair processes. Photomed Laser Surg. 2006;24:169-178. doi.org/10.1089/pho.2006.24.169.
Ozkan S, Isildar B, Oncul M, Baslar Z, Kaleli S, Koyuturk M. Ultrastructural analysis of human umbilical cord derived MSCs at undifferentiated stage and during osteogenic and adipogenic differentiation. Ultrastruct Pathol. 2018;42:199-210. doi.org/10.1080/01913123.2018.1453905.
Huang YY, Nagata K, Tedford CE, McCarthy T, Hamblin MR. Low-level laser therapy (LLLT) reduces oxidative stress in primary cortical neurons in vitro. J Biophotonics. 2013;6:829-838. doi.org/10.1002/jbio.201200157.
Anders JJ, Lanzafame RJ, Arany PR. Low-level light/laser therapy versus photobiomodulation therapy. Photomed Laser Surg. 2015;33:183-184. doi.org/10.1089/pho.2015.9848.
Bouvet-Gerbettaz S, Merigo E, Rocca JP, Carle GF, Rochet N. Effects of low-level laser therapy on proliferation and differentiation of murine bone marrow cells into osteoblasts and osteoclasts. Lasers Surg Med. 2009;41:291-297. doi.org/10.1002/lsm.20759.
Chen H, Wang H, Li Y, Liu W, Wang C, Chen Z. Biological effects of low-level laser irradiation on umbilical cord mesenchymal stem cells. Aip Adv. 2016;6:045018. doi.org/10.1063/1.4948442.
Karu T. Photobiology of low-power laser effects. Health Phys. 1989;56:691-704.
Min KH, Byun JH, Heo CY, Kim EH, Choi HY, Pak CS. Effect of Low-Level Laser Therapy on Human Adipose-Derived Stem Cells: In Vitro and In Vivo Studies. Aesthetic Plast Surg. 2015;39:778-782. doi.org/10.1007/s00266-015-0524-6.
de Andrade ALM, Luna GF, Brassolatti P, Leite MN, Parisi JR, de Oliveira Leal ÂM, Frade MAC, de Freitas Anibal F, Parizotto NA. Photobiomodulation effect on the proliferation of adipose tissue mesenchymal stem cells. Lasers Med Sci. 2019;34:677-683. doi.org/10.1007/s10103-018-2642-2.
Yang D, Yi W, Wang E, Wang M. Effects of light-emitting diode irradiation on the osteogenesis of human umbilical cord mesenchymal stem cells in vitro. Sci Rep. 2016;6:37370. doi.org/10.1038/srep37370.
Shimizu N, Mayahara K, Kiyosaki T, Yamaguchi A, Ozawa Y, Abiko Y. Low-intensity laser irradiation stimulates bone nodule formation via insulin-like growth factor-I expression in rat calvarial cells. Lasers Surg Med. 2007;39:551-559. doi.org/10.1002/lsm.20521.
Chen H, Wu H, Yin H, Wang J, Dong H, Chen Q, Li Y. Effect of photobiomodulation on neural differentiation of human umbilical cord mesenchymal stem cells. Lasers Med Sci. 2019;34:667-675. doi.org/10.1007/s10103-018-2638-y.
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