Photoelimination Potential of Chitosan Nanoparticles-Indocyanine Green Complex Against the Biological Activities of Acinetobacter baumannii Strains: A Preliminary In Vitro Study in Burn Wound Infections
Journal of Lasers in Medical Sciences,
Vol. 11 No. 2 (2020),
15 March 2020
,
Page 187-192
Abstract
Introduction: Acinetobacter baumannii strains are important agents causing serious nosocomial infections including soft-tissue and skin infections in patients with burn wounds which have become resistant to several classes of antibiotics. Antimicrobial photodynamic therapy (aPDT) as an alternative antimicrobial procedure is suggested for the treatment of these kinds of infections. The aim of the current study is to evaluate the antibacterial and anti-biofilm efficiency of aPDT by the utilization of an improved form of indocyanine green (ICG) which is encapsulated in chitosan nanoparticles (NCs@ICG).
Methods: NCs@ICG were synthesized and confirmed by the scanning electron microscope (SEM). aPDT was performed using NCs@ICG with an 810 nm wavelength of the diode laser at the fluency of 31.2 J/cm2 on 50 A. baumannii strains isolated from burn wounds. The antibacterial and anti-biofilm potential of NCs@ICG-aPDT was determined via the colony forming unit (CFU)/mL and crystal violet assays, respectively. In addition, microbial biofilm degradation was evaluated by the SEM.
Results: According to the results, NCs@ICG-aPDT showed a significant reduction of 93.2% on the CFU/mL of planktonic A. baumannii strains compared to the control group (untreated group; P < 0.05). In addition, the biofilm formation of A. baumannii strains was significantly reduced by 55.3% when the bacteria were exposed to NCs@ICG-aPDT (P < 0.05). In contrast, NCs@ICG, ICG, and the diode laser alone were not able to inhibit the CFU/mL and biofilm of A. baumannii strains (P > 0.05). Based on the results of SEM images, NCs@ICG-aPDT disrupted the biofilm structure of A. baumannii strains more than other groups.
Conclusion: NCs@ICG-aPDT demonstrates a promising treatment candidate for exploitation in wound infections against both planktonic and biofilm forms of A. baumannii strains.
- Antimicrobial photodynamic therapy
- Biofilms
- Chitosan nanoparticles
- Indocyanine green
- Acinetobacter baumannii.
How to Cite
References
Karaiskos I, Galani L, Baziaka F, Giamarellou H. Intraventricular and intrathecal colistin as the last
therapeutic resort for the treatment of multidrug-resistant and extensively drug-resistant Acinetobacter
baumannii ventriculitis and meningitis: a literature review. Int J Antimicrob Agents. 2013;41(6):499-508.
doi: 10.1016/j.ijantimicag.2013.02.006.
Cerqueira GM, Peleg AY. Insights into Acinetobacter baumannii pathogenicity. IUBMB Life.
;63(12):1055-1060. doi: 10.1002/iub.533.
Wisplinghoff H, Perbix W, Seifert H. Risk factors for nosocomial bloodstream infections due to
Acinetobacter baumannii: a case-control study of adult burn patients. Clin Infect Dis. 1999;28(1):59-66.
doi: 10.1086/515067.
Perez F, Hujer AM, Hujer KM, Decker BK, Rather PN, Bonomo RA. Global challenge of multidrug-
resistant Acinetobacter baumannii. Antimicrob Agents Chemother. 2007;51(10):3471-84. doi:
1128/AAC.01464-06
Avila-Novoa MG, Solís-Velázquez OA, Rangel-López DE, González-Gómez JP, Guerrero-Medina PJ,
Gutiérrez-Lomelí M. Biofilm Formation and Detection of Fluoroquinolone- and Carbapenem-Resistant
Genes in Multidrug-Resistant Acinetobacter baumannii. Can J Infect Dis Med Microbiol.
;2019:3454907. doi: 10.1155/2019/3454907.
Da Cunda P, Iribarnegaray V, Papa-Ezdra R, Bado I, González MJ, Zunino P, et al. Characterization of
the Different Stages of Biofilm Formation and Antibiotic Susceptibility in a Clinical Acinetobacter
baumannii Strain. Microb Drug Resist. 2019 [A head of print]. doi: 10.1089/mdr.2019.0145.
Cosgaya C, Ratia C, Marí-Almirall M, Rubio L, Higgins PG, Seifert H, et al. In vitro and in vivo
Virulence Potential of the Emergent Species of the Acinetobacter baumannii (Ab) Group. Front
Microbiol. 2019;10:2429. doi: 10.3389/fmicb.2019.02429.
Han XB, Li HX, Jiang YQ, Wang H, Li XS, Kou JY, et al. Upconversion nanoparticle-mediated
photodynamic therapy induces autophagy and cholesterol efflux of macrophage-derived foam cells via
ROS generation. Cell Death Dis. 2017;8(6):e2864. doi: 10.1038/cddis.2017.242.
Beltes C, Economides N, Sakkas H, Papadopoulou C, Lambrianidis T. Evaluation of Antimicrobial
Photodynamic Therapy Using Indocyanine Green and Near-Infrared Diode Laser Against Enterococcus
faecalis in Infected Human Root Canals. Photomed Laser Surg. 2017;35(5):264-269. doi:
1089/pho.2016.4100.
Deotale SP, Dubey SR, Gattani DR. Photoactivated disinfection using indocyanine green
photosensitizer as an adjunct to regenerative periodontal therapy. J Dent Lasers. 2017;11(1):24-28. doi:
4103/jdl.jdl_2_17
Krajczewski J, Rucińska K, Townley HE, Kudelski A. Role of various nanoparticles in photodynamic
therapy and detection methods of singlet oxygen. Photodiagnosis Photodyn Ther. 2019;26:162-178. doi:
1016/j.pdpdt.2019.03.016.
Konecsni K, Low NH, Nickerson MT. Chitosan–tripolyphosphate submicron particles as the carrier of
entrapped rutin. Food Chem. 2012;134(4):1775-1779. doi: 10.1016/j.foodchem.2012.03.070.
Oruba Z, Łabuz P, Macyk W, Chomyszyn-Gajewska M. Periopathogens differ in terms of the
susceptibility to toluidine blue O-mediated photodynamic inactivation. Photodiagnosis Photodyn Ther.
;20:28-34. doi: 10.1016/j.pdpdt.2017.08.008.
Gupta N, Gandham N, Jadhav S, Mishra RN. Isolation and identification of Acinetobacter species
with special reference to antibiotic resistance. J Nat Sci Biol Med. 2015;6(1):159-162. doi: 10.4103/0976-
149116.
Govindarajan C, Ramasubramaniam S, Gomathi T, Devi AN, Sudha PN. Sorption studies of Cr (VI)
from aqueous solution using nanochitosan-carboxymethyl cellulose blend. Arch Appl Sci Res.
;3(4):127-138.
Miles AA, Misra SS, Irwin JO. The estimation of the bactericidal power of the blood. J Hyg (Lond).
;38(6):732-49. doi: 10.1017/s002217240001158x.
Omar GS, Wilson M, Nair SP. Lethal photosensitization of wound-associated microbes using
indocyanine green and near-infrared light. BMC Microbiol. 2008;8:111. doi: 10.1186/1471-2180-8-111.
Pourhajibagher M, Chiniforush N, Ghorbanzadeh R, Bahador A. Photo-activated disinfection based
on indocyanine green against cell viability and biofilm formation of Porphyromonas gingivalis.
Photodiagnosis Photodyn Ther. 2017;17:61-64. doi: 10.1016/j.pdpdt.2016.10.003.
Pourhajibagher M, Ghorbanzadeh R, Bahador A. Investigation of arginine A-specific cysteine
proteinase gene expression profiling in clinical Porphyromonas gingivalis isolates against photokilling
action of the photo-activated disinfection. Lasers Med Sci. 2018;33(2):337-341. doi: 10.1007/s10103-
-2386-4.
Boehm TK, Ciancio SG. Diode laser activated indocyanine green selectively kills bacteria. J Int Acad
Periodontol. 2011;13(2):58-63
Fekrazad R, Khoei F, Hakimiha N, Bahador A. Photoelimination of Streptococcus mutans with two
methods of photodynamic and photothermal therapy. Photodiagnosis Photodyn Ther. 2013;10(4):626-
doi: 10.1016/j.pdpdt.2013.07.002.
Kranz S, Huebsch M, Guellmar A, Voelpel A, Tonndorf-Martini S, Sigusch BW. Antibacterial
photodynamic treatment of periodontopathogenic bacteria with indocyanine green and near-infrared laser
light enhanced by Trolox (TM). Lasers Surg Med. 2015;47(4):350-360. doi: 10.1002/lsm.22336.
Nagahara A, Mitani A, Fukuda M, Yamamoto H, Tahara K, Morita I, et al. Antimicrobial
photodynamic therapy using a diode laser with a potential new photosensitizer, indocyanine green-loaded
nanospheres, may be effective for the clearance of Porphyromonas gingivalis. J Periodontal Res.
;48(5):591-599. doi: 10.1111/jre.12042.
Topaloglu N, Gulsoy M, Yuksel S. Antimicrobial photodynamic therapy of resistant bacterial strains
by indocyanine green and 809-nm diode laser. Photomed Laser Surg. 2013;31(4):155-162. doi:
1089/pho.2012.3430.
Wong TW, Wu EC, Ko WC, Lee CC, Hor LI, Huang IH. Photodynamic inactivation of methicillin-
resistant Staphylococcus aureus by indocyanine green and near infrared light. Dermatologica sinica.
;36(1):8-15. doi.org/10.1016/j.dsi.2017.08.003.
Pourhajibagher M, Bahador A. Gene expression profiling of fimA gene encoding fimbriae among
clinical isolates of Porphyromonas gingivalis in response to photo-activated disinfection therapy.
Photodiagnosis Photodyn Ther. 2017;20:1-5. doi: 10.1016/j.pdpdt.2017.08.001.
Sasaki Y, Hayashi JI, Fujimura T, Iwamura Y, Yamamoto G, Nishida E, et al. New Irradiation
Method with Indocyanine Green-Loaded Nanospheres for Inactivating Periodontal Pathogens. Int J Mol
Sci. 2017;18(1). doi: 10.3390/ijms18010154.
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