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  3. Vol. 10 No. 3 (2019): Summer
  4. Original Article

Vol. 10 No. 3 (2019)

Tir 2019

Low-Power Red and Infrared Laser Effects on Cells Deficient in DNA Repair

  • Lucas Kiyoshi Fonseca Iwahara
  • Flavia Paoli
  • Adenilson Souza Fonseca

Journal of Lasers in Medical Sciences, Vol. 10 No. 3 (2019), 6 Tir 2019 , Page 157-162
Published: 2019-07-06

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Abstract

Introduction: Low-level lasers are successfully used to prevent and treat diseases in soft oral and bone tissues, particularly diseases in oral cavity caused by chemotherapy and radiotherapy in oncology. However, controversy exists as to whether these lasers induce molecular side effects, mainly on DNA. The aim of this work was to assess the effects of low-power lasers on mutant Escherichia coli cells in DNA repair.

Methods: Escherichia coli wild type cultures as well as those lacking recombination DNA repair (recA-) and la SOS responses (lexA-) irradiated with lasers at different energy densities, powers, and emission modes for cell viability and morphology assessment were used in this study.

Results: Laser irradiation: (i) did not affect cell viability of non-mutant and lexA- cells but decreased viability in recA- cultures; (ii) altered morphology of wild type and lexA, depending on the energy density, power, emission mode, and wavelength.

Conclusion: Results show that low-level lasers have lethal effects on both recombination DNA repair and SOS response bacterial cells but do not induce morphological modifications in these cells.


Keywords:
  • DNA repair
  • Escherichia coli
  • Laser therapy.
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How to Cite

Fonseca Iwahara, L. K., Paoli, F., & Fonseca, A. S. (2019). Low-Power Red and Infrared Laser Effects on Cells Deficient in DNA Repair. Journal of Lasers in Medical Sciences, 10(3), 157–162. Retrieved from https://journals.sbmu.ac.ir/jlms/article/view/16926
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References

Niemz MH. Laser-tissue interactions: Fundamentals and applications. Springer-Verlag: New York; 2007.

Peplow PV, Chung TY, Baxter GD. Laser Photobiomodulation of wound healing: a review of experimental studies in mouse and rat animal models. Photomed Laser Surg. 2010;28:291-325.

Maia ML, Bonjardim LR, Quintans Jde S, Ribeiro MA, Maia LG, Conti PC. Effect of low-level laser therapy on pain levels in patients with temporomandibular disorders: a systematic review. J Appl Oral Sci. 2012;20:594-602.

Gross AR, Dziengo S, Boers O, Goldsmith CH, Graham N, Lilge L, Burnie S, White R. Low Level Laser Therapy (LLLT) for Neck Pain: A Systematic Review and Meta-Regression. Open Orthop J. 2013;7:396-419.

Karu T. Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol, Biol. 1999;49:1-17.

Karu T, Pyatibrat L. Gene expression under laser and light-emitting diodes radiation for modulation of cell adhesion: possible applications for biotechnology. IUBMB Life. 2011;63:747-53.

Hawkins DH, Abrahamse H. The role of laser fluence in cell viability, proliferation, and membrane integrity of wounded human skin fibroblasts following helium-neon laser irradiation. Lasers Surg Med. 2006;38:74-83.

Kim YG. Laser mediated production of reactive oxygen and nitrogen species; implications for therapy. Free Radic Res. 2002;36:1243-50.

Godon C, Cordelières FP, Biard D, Giocanti N, Mégnin-Chanet F, Hall J, Favaudon V. PARP inhibition versus PARP-1 silencing: different outcomes in terms of single-strand break repair and radiation susceptibility. Nucleic Acids Res. 2008;36:4454-64.

Mbene AB, Houreld NN, Abrahamse HJ. DNA damage after phototherapy in wounded fibroblast cells irradiated with 16 J/cm(2). J Photochem Photobiol B: Biol. 2009; 94:131-7.

Kohli R, Gupta PK. Irradiance dependence of the He-Ne laser-induced protection against UVC radiation in E. coli strains. J Photochem Photobiol B: Biol. 2003;69:161-7.

Fonseca AS, Geller M, Filho MB, Valença SS, Paoli F. Low-level infrared laser effect on plasmid DNA. Lasers Med Sci. 2012;27:121-30.

Fonseca AS, Moreira TO, Paixão DL, Farias FM, Guimarães OR, Paoli S, Geller M, Paoli F. Effect of laser therapy on DNA damage. Lasers Surg Med. 2010;42:481-8.

Teixeira GR, Sergio LPS, Marciano RS,Polignano GAC, Guimarães OR, Geller M,Paoli F, Fonseca AS. Infrared laser effects at fluences used for treatment of dentin hypersensitivity on DNA repair in Escherichia coli and plasmids. Opt Laser Technol. 2014; 64:46-52. doi: 10.1016/j.optlastec.2014.04.023

Fonseca AS, Presta GA, Geller M, Paoli F. Low intensity infrared laser induces filamentation in Escherichia coli cells. Lasers Phys. 2011;21:1-9.

Marciano RS, Sergio LPS, Polignano GAC, Presta GA, Guimarães OR, Geller M, Paoli S, Paoli F, Fonseca AS. Laser for treatment of aphthous ulcers on bacteria cultures and DNA. Photochem Photobiol Sci. 2012;11:1476-83.

Cappuccino JG, Sherman N. Microbiology: a laboratory manual. Benjamin Cummings Science Publishing: California,1999.

Kacinski BM, Rupp WD, Ludlum DB. Repair of haloethylnitrosourea-induced DNA damage in mutant and adapted bacteria. Cancer Res. 1985;45(12 Pt 1):6471-4.

Koch WH, Woodgate R. The SOS response. In: Nickoloff JA, Hoekstra MF. (Eds). DNA Damage and Repair. Volume I: DNA Repair in Procaryotes and Lower Eukaryotes. New Jersey: Humana Press; 1998. 107-34.

Canuto KS, Sergio LPS, Marciano RS, Guimarães OR, Polignano GAC, Geller M, Paoli F, Fonseca AS. DNA repair in bacterial cultures and plasmid DNA exposed to infrared laser for treatment of pain. Laser Phys Lett. 2013;10:065606.

Ignatov YD, Vislobokov AI, Vlasov TD, Kolpakova ME, Mel'nikov KN, Petrishchev IN. Effects of helium-neon laser irradiation and local anesthetics on potassium channels in pond snail neurons. Neurosci Behav Physiol. 2005;35:871-5.

Giannelli M, Chellini F, Sassoli C, Francini F, Pini A, Squecco R, Nosi D, Bani D, Zecchi-Orlandini S, Formigli L. Photoactivation of bone marrow mesenchymal stromal cells with diode laser: effects and mechanisms of action. J Cell Physiol. 2013;228:172-81.

Huang YY, Nagata K, Tedford CE, Hamblin MR. Low-level laser therapy (810 nm) protects primary cortical neurons against excitotoxicity in vitro. J Biophotonics. 2014;7:656-64.

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