Effects of Root Debridement With Hand Curettes and Er:YAG Laser on Chemical Properties and Ultrastructure of Periodontally-Diseased Root Surfaces Using Spectroscopy and Scanning Electron Microscopy
Journal of Lasers in Medical Sciences,
Vol. 8 No. 2 (2017),
8 April 2017
,
Page 66-71
Abstract
Introduction: The efficacy of erbium-doped yttrium aluminum garnet (Er:YAG) laser for root debridement in comparison with curettes has been the subject of many recent investigations. Considering the possibility of chemical and ultra-structural changes in root surfaces following laser irradiation, this study sought to assess the effects of scaling and root planing (SRP) with curettes and Er:YAG laser on chemical properties and ultrastructure of root surfaces using spectroscopy and scanning electron microscopy (SEM).
Methods: In this in vitro experimental study, extracted sound human single-rooted teeth (n = 50) were randomly scaled using manual curettes alone or in conjunction with Er:YAG laser at 100 and 150 mJ/pulse output energies. The weight percentages of carbon, oxygen, phosphorous and calcium remaining on the root surfaces were calculated using spectroscopy and the surface morphology of specimens was assessed under SEM. Data were analyzed using one-way analysis of variance (ANOVA).
Results: No significant differences (P > 0.05) were noted in the mean carbon, oxygen, phosphorous and calcium weight percentages on root surfaces following SRP using manual curettes with and without laser irradiation at both output energies. Laser irradiation after SRP with curettes yielded rougher surfaces compared to the use of curettes alone.
Conclusion: Although laser irradiation yielded rougher surfaces, root surfaces were not significantly different in terms of chemical composition following SRP using manual curettes with and without Er:YAG laser irradiation. Er:YAG laser can be safely used as an adjunct to curettes for SRP.
- Absorption spectroscopy
- Nonsurgical periodontal treatment
- Erbium YAG Laser
- Electron scanning microscopy
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References
Smart GJ, Wilson M, Davies EH, Kieser JB. The assessment of ultrasonic root surface debridement by determination of residual endotoxin levels. J Clin Periodontol. 1990;17(3):174-178.
Walmsley AD, Lea SC, Landini G, Moses AJ. Advances in power driven pocket/root instrumentation. J Clin Periodontol. 2008;35(8 suppl):22-28. doi:10.1111/j.1600- 051X.2008.01258.x.
Maruyama H, Aoki A, Sasaki KM, et al. The effect of chemical and/or mechanical conditioning on the Er:YAG laser-treated root cementum: analysis of surface morphology and periodontal ligament fibroblast attachment. Lasers Surg Med. 2008;40(3):211-222. doi:10.1002/lsm.20609.
de Mendonca AC, Maximo MB, Rodrigues JA, Arrais CA, de Freitas PM, Duarte PM. Er:YAG Laser, ultrasonic system, and curette produce different profiles on dentine root surfaces: an in vitro study. Photomed Laser Surg. 2008;26(2):91-97. doi:10.1089/pho.2007.2129.
Ting CC, Fukuda M, Watanabe T, Aoki T, Sanaoka A, Noguchi T. Effects of Er,Cr:YSGG laser irradiation on the root surface: morphologic analysis and efficiency of calculus removal. J Periodontol. 2007;78(11):2156-2164. doi:10.1902/jop.2007.070160.
Castro GL, Gallas M, Nunez IR, Borrajo JL, Varela LG. Histological evaluation of the use of diode laser as an adjunct to traditional periodontal treatment. Photomed Laser Surg. 2006;24(1):64-68. doi:10.1089/pho.2006.24.64.
Theodoro LH, Haypek P, Bachmann L, et al. Effect of ER:YAG and diode laser irradiation on the root surface: morphological and thermal analysis. J Periodontol. 2003;74(6):838-843. doi:10.1902/jop.2003.74.6.838.
Schwarz F, Aoki A, Sculean A, Georg T, Scherbaum W, Becker J. In vivo effects of an Er:YAG laser, an ultrasonic system and scaling and root planing on the biocompatibility of periodontally diseased root surfaces in cultures of human PDL fibroblasts. Lasers Surg Med. 2003;33(2):140- 147. doi:10.1002/lsm.10201.
Altundasar E, Ozcelik B, Cehreli ZC, Matsumoto K. Ultramorphological and histochemical changes after ER,CR:YSGG laser irradiation and two different irrigation regimes. J Endod. 2006;32(5):465-468. doi:10.1016/j. joen.2005.08.005.
Lin CP, Lee BS, Lin FH, Kok SH, Lan WH. Phase, compositional, and morphological changes of human dentin after Nd:YAG laser treatment. J Endod. 2001;27(6):389- 393. doi:10.1097/00004770-200106000-00004.
Hossain M, Kimura Y, Nakamura Y, Yamada Y, Kinoshita JI, Matsumoto K. A study on acquired acid resistance of enamel and dentin irradiated by Er,Cr:YSGG laser. J Clin Laser Med Surg. 2001;19(3):159-163. doi:10.1089/10445470152927991.
Hossain M, Nakamura Y, Kimura Y, Ito M, Yamada Y, Matsumoto K. Acquired acid resistance of dental hard tissues by CO2 laser irradiation. J Clin Laser Med Surg. 1999;17(5):223-226.
Hara AT, Ando M, Cury JA, Serra MC, Gonzalez-Cabezas C, Zero DT. Influence of the organic matrix on root dentine erosion by citric acid. Caries Res. 2005;39(2):134-138. doi:10.1159/000083159.
de-Melo MA, Passos VF, Alves JJ, Barros EB, Santiago SL, Rodrigues LK. The effect of diode laser irradiation on dentin as a preventive measure against dental erosion: an in vitro study. Lasers Med Sci. 2011;26(5):615-621. doi:10.1007/s10103-010-0865-y.
Paula Sde S, Soares LE, do Espirito Santo AM, Martin AA, Cavalli V, Liporoni PC. FT-Raman and energy dispersive X-ray fluorescence spectrometric analyses of enamel submitted to 38% hydrogen peroxide bleaching, an acidic beverage, and simulated brushing. Photomed Laser Surg. 2010;28(3):391-396. doi:10.1089/pho.2008.2426.
Folwaczny M, George G, Thiele L, Mehl A, Hickel R. Root surface roughness following Er:YAG laser irradiation at different radiation energies and working tip angulations. J Clin Periodontol. 2002;29(7):598-603.
Kocher T, Rosin M, Langenbeck N, Bernhardt O. Subgingival polishing with a teflon-coated sonic scaler insert in comparison to conventional instruments as assessed on extracted teeth (II). Subgingival roughness. J Clin Periodontol. 2001;28(8):723-729.
Theodoro LH, Sampaio JE, Haypek P, Bachmann L, Zezell DM, Garcia VG. Effect of Er:YAG and Diode lasers on the adhesion of blood components and on the morphology of irradiated root surfaces. J Periodontal Res. 2006;41(5):381- 390. doi:10.1111/j.1600-0765.2005.00846.x.
de Oliveira GJ, Sampaio JE, Marcantonio RA. Effects of Er,Cr:YSGG laser irradiation on root surfaces for adhesion of blood components and morphology. Photomed Laser Surg. 2010;28(6):751-756. doi:10.1089/pho.2009.2681.
Foroutan T, Amid R, Karimi MR. Comparison of Manual Tools, Ultrasonic and Erbium-Doped Yttrium Aluminum Garnet (Er:YAG) Laser on the Debridement Effect of the Surface of the Root of Teeth Suffering from Periodontitis. J Lasers Med Sci. 2013;4(4):199-205.
Amid R, Kadkhodazadeh M, Fekrazad R, Hajizadeh F, Ghafoori A. Comparison of the effect of hand instruments, an ultrasonic scaler, and an erbium-doped yttrium aluminium garnet laser on root surface roughness of teeth with periodontitis: a profilometer study. J Periodontal Implant Sci. 2013;43(2):101-105. doi:10.5051/ jpis.2013.43.2.101.
Birang R, Poursamimi J, Gutknecht N, Lampert F, Mir M. Comparative evaluation of the effects of Nd:YAG and Er:YAG laser in dentin hypersensitivity treatment. Lasers Med Sci. 2007;22(1):21-24. doi:10.1007/s10103-006- 0412-z.
Schwarz F, Sculean A, Berakdar M, Szathmari L, Georg T, Becker J. In vivo and in vitro effects of an Er:YAG laser, a GaAlAs diode laser, and scaling and root planing on periodontally diseased root surfaces: a comparative histologic study. Lasers Surg Med. 2003;32(5):359-366. doi:10.1002/lsm.10179.
Frentzen M, Braun A, Aniol D. Er:YAG laser scaling of diseased root surfaces. J Periodontol. 2002;73(5):524-530. doi:10.1902/jop.2002.73.5.524.
Crespi R, Barone A, Covani U. Er:YAG laser scaling of diseased root surfaces: a histologic study. J Periodontol. 2006;77(2):218-222. doi:10.1902/jop.2006.050043.
Tsurumaki Jdo N, Souto BH, Oliveira GJ, Sampaio JE, Marcantonio Junior E, Marcantonio RA. Effect of instrumentation using curettes, piezoelectric ultrasonic scaler and Er,Cr:YSGG laser on the morphology and adhesion of blood components on root surfaces: a SEM study. Braz Dent J. 2011;22(3):185-192.
Vastardis S, Yukna RA, Rice DA, Mercante D. Root surface removal and resultant surface texture with diamond-coated ultrasonic inserts: an in vitro and SEM study. J Clin Periodontol. 2005;32(5):467-473. doi:10.1111/j.1600- 051X.2005.00705.x.
Blumenthal NM, Singiser RT. The enhancement of guided tissue regeneration by altering root surface topography. Int J Periodontics Restorative Dent. 1993;13(4):360-371.
Oberholzer R, Rateitschak KH. Root cleaning or root smoothing. An in vivo study. J Clin Periodontol. 1996;23(4):326-330.
Leknes KN, Lie T, Wikesjo UM, Boe OE, Selvig KA. Influence of tooth instrumentation roughness on gingival tissue reactions. J Periodontol. 1996;67(3):197-204. doi:10.1902/jop.1996.67.3.197.
Leknes KN, Lie T, Wikesjo UM, Bogle GC, Selvig KA. Influence of tooth instrumentation roughness on subgingival microbial colonization. J Periodontol. 1994;65(4):303-308. doi:10.1902/jop.1994.65.4.303.
Casarin RC, Ribeiro FV, Sallum AW, Sallum EA, Nociti- Jr FH, Casati MZ. Root surface defect produced by hand instruments and ultrasonic scaler with different power settings: an in vitro study. Braz Dent J. 2009;20(1):58-63.
Kocher T, Langenbeck N, Rosin M, Bernhardt O. Methodology of three-dimensional determination of root surface roughness. J Periodontal Res. 2002;37(2):125-131.
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