Effects of Ginger on Advanced Glycation End-Products and Inflammation in the Ovarian Tissue of Streptozotocin-Induced Diabetic Rats
Archives of Medical Laboratory Sciences,
Vol. 8 (2022),
10 January 2022
,
Page 1-8 (e8)
https://doi.org/10.22037/amls.v8.38729
Abstract
Background and Aim: Advanced glycation end products (AGEs) and inflammation play a crucial role in the progression of diabetic complications, including ovarian disorders. The current research investigated the potential impact of hydroalcoholic extract of ginger (Zingiber Officinale) on hyperglycemia-induced AGEs and inflammatory markers.
Methods: A total of 96 female Wistar rats were randomized into four groups (n=24 in each group) as follows: 1) control, 2) diabetes, 3) diabetes + 200 mg/kg ginger, 4) diabetes + 400 mg/kg ginger. Streptozotocin (STZ) - induced diabetic rats, as our experimental model for diabetes, orally received 200 or 400 mg/kg/day ginger extract for eight weeks. At the end of the treatment period, body weight, ovarian weight, serum AGEs level, ovarian RAGE, IL-1β, and TNF-α mRNA levels were measured.
Results: At the end of the study, diabetic rats exhibited an obvious decrease in body weight (P < 0.01) and ovarian weight (P < 0.01) compared to normal rats. However, ginger supplementation (200 mg/kg) exhibited a significant increment in ovarian weight (P < 0.05) and body weight (P < 0.01). These changes were also more pronounced in the diabetic rats treated with 400 mg/kg ginger extract (P < 0.01). Serum AGEs (P < 0.001) and ovarian RAGE (P < 0.01), IL-1β (P < 0.01), and TNF-α (P < 0.01) mRNA levels were significantly elevated in diabetic rats compared with control group. Administration of the diabetic group with 200 mg/kg ginger extract significantly ameliorated the serum level of AGEs (P < 0.01) and the transcript levels of RAGE (P < 0.05), TNF-α (P < 0.01) and IL-1β (P < 0.05). The 400 mg/kg ginger extract dose remarkably alleviated AGEs (P < 0.001) in the serum and RAGE (P < 0.01), TNF-α (P < 0.01), and IL-1β (P < 0.01) in the ovary of diabetic rats.
Conclusion: The present study's findings revealed that daily administration of ginger extract reduces AGEs and the transcript levels of RAGE and inflammatory markers in the STZ- induced female rats.
*Corresponding Author: Tohid Moradi Gardeshi; Email: d.tohidmoradi@iau-garmsar.ac.ir; ORCID ID: 0000-0002-3366-0211
Please cite this article as: Hasani SK, Pourmahdi O, Fallah Raoufi M, Moradi Gardeshi T. Effects of Ginger on Advanced Glycation End Products and Inflammation in the Ovarian Tissue of Streptozotocin-Induced Diabetic Rats. Arch Med Lab Sci. 2022;8:1-7 (e8). https://doi.org/10.22037/amls.v8.38729
- Diabetes,
- Ovary
- Advanced Glycation End Products
- Streptozotocin
- Inflammation
How to Cite
References
Association AD. Introduction: standards of medical care in diabetes—2020. Am Diabetes Assoc; 2020. p. S1-S2.
Forouhi NG, Wareham NJ. Epidemiology of diabetes. Medicine. 2019;47(1):22-7.
Eckel RH, Bornfeldt KE, Goldberg IJ. Cardiovascular disease in diabetes, beyond glucose. Cell Metabolism. 2021;33(8):1519-45.
Hill-Briggs F, Adler NE, Berkowitz SA, Chin MH, Gary-Webb TL, Navas-Acien A, et al. Social determinants of health and diabetes: a scientific review. Diabetes Care. 2021;44(1):258-79.
Lin P-H, Chang C-C, Wu K-H, Shih C-K, Chiang W, Chen H-Y, et al. Dietary glycotoxins, advanced glycation end products, inhibit cell proliferation and progesterone secretion in ovarian granulosa cells and mimic PCOS-like symptoms. Biomolecules. 2019;9(8):327.
Zhu J-l, Cai Y-q, Long S-l, Chen Z, Mo Z-c. The role of advanced glycation end products in human infertility. Life Sciences. 2020;255:117830.
Fattah A, Morovati A, Niknam Z, Mashouri L, Asadi A, Rizi ST, et al. The Synergistic Combination of Cisplatin and Piperine Induces Apoptosis in MCF-7 Cell Line. Iranian Journal of Public Health. 2021;50(5):1037-47.
Perrone A, Giovino A, Benny J, Martinelli F. Advanced glycation end products (AGEs): biochemistry, signaling, analytical methods, and epigenetic effects. Oxidative medicine and cellular longevity. 2020;2020.
Nowotny K, Schröter D, Schreiner M, Grune T. Dietary advanced glycation end products and their relevance for human health. Aging research reviews. 2018;47:55-66.
Maleki N, Ravesh RK, Salehiyeh S, Faiz AF, Ebrahimi M, Sharbati A, et al. Comparative effects of estrogen and silibinin on cardiovascular risk biomarkers in ovariectomized rats. Gene. 2022;823:146365.
Zhang Q, Wang Y, Fu L. Dietary advanced glycation end‐products: Perspectives linking food processing with health implications. Comprehensive Reviews in Food Science and Food Safety. 2020;19(5):2559-87.
Le Bagge S, Fotheringham AK, Leung SS, Forbes JM. Targeting the receptor for advanced glycation end products (RAGE) in type 1 diabetes. Medicinal research reviews. 2020;40(4):1200-19.
Musavi H, Abazari O, Safaee MS, Variji A, Koohshekan B, Kalaki-Jouybari F, et al. Mechanisms of COVID-19 Entry into the Cell: Potential Therapeutic Approaches Based on Virus Entry Inhibition in COVID-19 Patients with Underlying Diseases. Iranian journal of allergy, asthma, and immunology. 2021;20(1):11-23.
Cepas V, Collino M, Mayo JC, Sainz RM. Redox signaling and advanced glycation endproducts (AGEs) in diet-related diseases. Antioxidants. 2020;9(2):142.
Parwani K, Mandal P. Role of advanced glycation end products and insulin resistance in diabetic nephropathy. Archives of physiology and biochemistry. 2020:1-13.
Sharifat N, Jafari-Hafshejani F, Dayati P, Lorestanpoor P, Paydar A, Babaahmadi Rezaei H. Inhibitory effect of Curcumin on phosphorylation NFκB-p65 induced by hydrogen peroxide in Bovine Endothelial Cells. Journal of Fasa University of Medical Sciences. 2017;7(2):283-90.
Artimani T, Karimi J, Mehdizadeh M, Yavangi M, Khanlarzadeh E, Ghorbani M, et al. Evaluation of pro-oxidant-antioxidant balance (PAB) and its association with inflammatory cytokines in polycystic ovary syndrome (PCOS). Gynecological endocrinology. 2018;34(2):148-52.
Panji M, Behmard V, Zare Z, Malekpour M, Nejadbiglari H, Yavari S, et al. Synergistic effects of green tea extract and paclitaxel in the induction of mitochondrial apoptosis in ovarian cancer cell lines. Gene. 2021;787:145638.
Jacob B, Narendhirakannan R. Role of medicinal plants in the management of diabetes mellitus: a review. 3 Biotech. 2019;9(1):1-17.
Zhu J, Chen H, Song Z, Wang X, Sun Z. Effects of ginger (Zingiber officinale Roscoe) on type 2 diabetes mellitus and components of the metabolic syndrome: A systematic review and meta-analysis of randomized controlled trials. Evidence-based complementary and alternative medicine. 2018;2018.
Shahidi M, Moradi A, Dayati P. Zingerone attenuates zearalenone-induced steroidogenesis impairment and apoptosis in TM3 Leydig cell line. Toxicon. 2022;211:50-60.
Lamuchi-Deli N, Aberomand M, Babaahmadi-Rezaei H, Mohammadzadeh G. Effects of the hydroalcoholic extract of Zingiber officinale on arginase i activity and expression in the retina of streptozotocin-induced diabetic rats. International Journal of Endocrinology and Metabolism. 2017;15(2).
Tzeng T-F, Liou S-S, Chang CJ, Liu I-M. Zerumbone, a tropical ginger sesquiterpene, ameliorates streptozotocin-induced diabetic nephropathy in rats by reducing the hyperglycemia-induced inflammatory response. Nutrition & metabolism. 2013;10(1):1-12.
Abdi T, Mahmoudabady M, Marzouni HZ, Niazmand S, Khazaei M. Ginger (Zingiber Officinale Roscoe) extract protects the heart against inflammation and fibrosis in diabetic rats. Canadian Journal of Diabetes. 2021;45(3):220-7.
Kalousova M, Skrha J, Zima T. Advanced glycation end-products and advanced oxidation protein products in patients with diabetes mellitus. Physiological research. 2002;51(6):597-604.
Kim C, Miller R, Braffett B, Pan Y, Arends V, Saenger A, et al. Ovarian markers and irregular menses among women with type 1 diabetes in the Epidemiology of Diabetes Interventions and Complications study. Clinical endocrinology. 2018;88(3):453-9.
Thong EP, Codner E, Laven JS, Teede H. Diabetes: a metabolic and reproductive disorder in women. The Lancet Diabetes & Endocrinology. 2020;8(2):134-49.
Panji M, Behmard V, Zare Z, Malekpour M, Nejadbiglari H, Yavari S, et al. Suppressing effects of Green tea extract and Epigallocatechin-3-gallate (EGCG) on TGF-β-induced Epithelial-to-mesenchymal transition via ROS/Smad signaling in human cervical cancer cells. Gene. 2021:145774.
Arablou T, Aryaeian N, Valizadeh M, Sharifi F, Hosseini A, Djalali M. The effect of ginger consumption on glycemic status, lipid profile and some inflammatory markers in patients with type 2 diabetes mellitus. International journal of food sciences and nutrition. 2014;65(4):515-20.
Shidfar F, Rajab A, Rahideh T, Khandouzi N, Hosseini S, Shidfar S. The effect of ginger (Zingiber officinale) on glycemic markers in patients with type 2 diabetes. Journal of Complementary and Integrative Medicine. 2015;12(2):165-70.
Wang-Fischer Y, Garyantes T. Improving the reliability and utility of streptozotocin-induced rat diabetic model. Journal of diabetes research. 2018;2018.
Wu J, Yan L-J. Streptozotocin-induced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic β cell glucotoxicity. Diabetes, metabolic syndrome and obesity: targets and therapy. 2015;8:181.
Pourgholi M, Abazari O, Pourgholi L, Ghasemi-Kasman M, Boroumand M. Association between rs3088440 (G> A) polymorphism at 9p21. 3 locus with the occurrence and severity of coronary artery disease in an Iranian population. Molecular Biology Reports. 2021;48(8):5905-12.
Zhang Y, Mei H, Shan W, Shi L, Chang X, Zhu Y, et al. Lentinan protects pancreatic β cells from STZ‐induced damage. Journal of cellular and molecular medicine. 2016;20(10):1803-12.
Subash-Babu P, Alshatwi AA, Ignacimuthu S. Beneficial antioxidative and antiperoxidative effect of cinnamaldehyde protect streptozotocin-induced pancreatic β-cells damage in wistar rats. Biomolecules & therapeutics. 2014;22(1):47.
Hall R, Keeble L, Sünram-Lea S-I, To M. A review of risk factors associated with insulin omission for weight loss in type 1 diabetes. Clinical Child Psychology and Psychiatry. 2021;26(3):606-16.
Dekel Y, Glucksam Y, Elron-Gross I, Margalit R. Insights into modeling streptozotocin-induced diabetes in ICR mice. Lab animal. 2009;38(2):55-60.
Al Hroob AM, Abukhalil MH, Alghonmeen RD, Mahmoud AM. Ginger alleviates hyperglycemia-induced oxidative stress, inflammation and apoptosis and protects rats against diabetic nephropathy. Biomedicine & Pharmacotherapy. 2018;106:381-9.
Bi X, Lim J, Henry CJ. Spices in the management of diabetes mellitus. Food chemistry. 2017;217:281-93.
Adeniyi P, Sanusi R, Obatolu V. Dietary Ginger extracts enhanced glucose uptake by muscle and adipose of normal and diabetic rats via mimicry of insulin action. American Journal of Biomedical Research. 2017;5(3):46-56.
Zare Z, Dizaj TN, Lohrasbi A, Sheikhalishahi ZS, Asadi A, Zakeri M, et al. Silibinin inhibits TGF-β-induced MMP-2 and MMP-9 through Smad Signaling pathway in colorectal cancer HT-29 cells. Basic and Clinical Cancer Research. 2020;12(2):81-90.
Majd NE, Azizian H, Tabandeh MR, Shahriari A. Effect of Abelmoschus esculentus powder on ovarian histology, expression of apoptotic genes and oxidative stress in diabetic rats fed with high fat diet. Iranian journal of pharmaceutical research: IJPR. 2019;18(1):369.
Maleki N, Yavari N, Ebrahimi M, Faiz AF, Ravesh RK, Sharbati A, et al. Silibinin exerts anti-cancer activity on human ovarian cancer cells by increasing apoptosis and inhibiting epithelial-mesenchymal transition (EMT). Gene. 2022;823:146275.
Mehrabianfar P, Dehghani F, Karbalaei N, Mesbah F. The effects of metformin on stereological and ultrastructural features of the ovary in streptozotocin-induced diabetes adult rats: An experimental study. International Journal of Reproductive Biomedicine. 2020;18(8):651.
Diamanti-Kandarakis E, Piperi C, Patsouris E, Korkolopoulou P, Panidis D, Pawelczyk L, et al. Immunohistochemical localization of advanced glycation end-products (AGEs) and their receptor (RAGE) in polycystic and normal ovaries. Histochemistry and cell biology. 2007;127(6):581-9.
Garg D, Merhi Z. Relationship between advanced glycation end products and steroidogenesis in PCOS. Reproductive Biology and Endocrinology. 2016;14(1):1-13.
Zare Z, Dizaj TN, Lohrasbi A, Sheikhalishahi ZS, Panji M, Hosseinabadi F, et al. The Effect of Piperine on MMP-9, VEGF, and E-cadherin Expression in Breast Cancer MCF-7 Cell Line. Basic & Clinical Cancer Research. 2020;12(3).
Garg D, Merhi Z. Advanced glycation end products: link between diet and ovulatory dysfunction in PCOS? Nutrients. 2015;7(12):10129-44.
Spagnuolo L, Della Posta S, Fanali C, Dugo L, De Gara L. Antioxidant and antiglycation effects of polyphenol compounds extracted from hazelnut skin on advanced glycation end-products (ages) formation. Antioxidants. 2021;10(3):424.
Nonaka K, Bando M, Sakamoto E, Inagaki Y, Naruishi K, Yumoto H, et al. 6-Shogaol inhibits advanced glycation end-products-induced IL-6 and ICAM-1 expression by regulating oxidative responses in human gingival fibroblasts. Molecules. 2019;24(20):3705.
- Abstract Viewed: 222 times
- PDF Downloaded: 101 times