Serum Klotho Level and its Related Factors Among Male Opioids Addicts With Normal Renal Function Compared to Healthy Male Non-smokers and Smokers in Tabriz, Iran
International Journal of Medical Toxicology and Forensic Medicine,
Vol. 13 No. 3 (2023),
17 October 2023
https://doi.org/10.32598/ijmtfm.v13i3.42587
Abstract
Background: Klotho is an aging-suppressor gene that encodes a single-pass transmembrane protein and acts as a hormone. In this study, we aim to investigate the serum α-Klotho level in male opioids addicts with normal kidney function compared to healthy male non-smokers and smokers in Tabriz, Iran.
Methods: Participnts were 87 men with normal kidney function referred to Sina Educational Research and Treatment Center in Tabriz, Iran (29 opioids addicts, 29 healthy non-smokers, and 29 healthy smokers). Blood samples were collected to measure the soluble a-Klotho level using an ELISA kit. Furthermore, blood creatinine (Cr) and hemoglobin (Hb) levels was measured. Body mass index (BMI) was also calculated for all participants.
Results: In addicts, BMI, Hb, and Cr levels were significantly lower than in healthy non-smokers and smokers, but their Klotho level was higher (P>0.05). The Klotho level in healthy smokers was significantly lower than in healthy non-smokers and addicts. The Klotho level of healthy smokers decreased as the pack year increased, but the duration of opioid addiction had no significant association with the Klotho level. There was no significant difference in the Klotho level between control groups (non-smokers and smokers) and men with addiction to different types of opioids.
Conclusion: The Klotho level in male opioid addicts is significantly higher than in smokers. There is a significant negative correlation between BMI and Klotho levels among men with normal BMI and overweight. Further studies are recommended in these fields.
- Klotho
- Opioid
- Addicts
- Smokers
- Renal function
- 09/30/2023 (2)
- 09/25/2023 (1)
How to Cite
References
Kuro-o M. Klotho. Pflügers Archiv-European Journal of Physiology. 2010; 459(2):333-43. [DOI:10.1007/s00424-009-0722-7] [PMID]
Dalton GD, Xie J, An SW, Huang CL. New insights into the mechanism of action of soluble klotho. Frontiers in Endocrinology. 2017; 8:323. [DOI:10.3389/fendo.2017.00323] [PMID] [PMCID]
Patel MS, Donaldson AV, Lewis A, Natanek SA, Lee JY, Andersson YM, et al. Klotho and smoking-An interplay influencing the skeletal muscle function deficits that occur in COPD. Respiratory Medicine. 2016; 113:50-6. [DOI:10.1016/j.rmed.2016.02.004] [PMID]
Lim K, Groen A, Molostvov G, Lu T, Lilley KS, Snead D, et al. α-Klotho expression in human tissues. The Journal of Clinical Endocrinology & Metabolism. 2015; 100(10):E1308-E18. [DOI:10.1210/jc.2015-1800] [PMID] [PMCID]
Buchanan S, Combet E, Stenvinkel P, Shiels PG. Klotho, aging, and the failing kidney. Frontiers in Endocrinology. 2020; 11:560. [DOI:10.3389/fendo.2020.00560] [PMID] [PMCID]
Yang K, Yang J, Bi X, Yu Z, Xiao T, Huang Y, et al. Serum Klotho, cardiovascular events, and mortality in nondiabetic chronic kidney disease. Cardiorenal Medicine. 2020; 10(3):175-87. [DOI:10.1159/000506380] [PMID]
Hu MC, Kuro-o M, Moe OW. The emerging role of Klotho in clinical nephrology. Nephrology Dialysis Transplantation. 2012; 27(7):2650-7. [DOI:10.1093/ndt/gfs160] [PMID] [PMCID]
Nakanishi K, Nishida M, Harada M, Ohama T, Kawada N, Murakami M, et al. Klotho-related molecules upregulated by smoking habit in apparently healthy men: A cross-sectional study. Scientific Reports. 2015; 5:14230. [DOI:10.1038/srep14230] [PMID] [PMCID]
Kamizono Y, Shiga Y, Suematsu Y, Imaizumi S, Tsukahara H, Noda K, et al. Impact of cigarette smoking cessation on plasma α-klotho levels. Medicine. 2018; 97(35):e11947. [DOI:10.1097/MD.0000000000011947] [PMID] [PMCID]
Verde Z, González-Moro JMR, Chicharro LM, Reinoso-Barbero L, Bandrés F, Gómez-Gallego F, et al. A Paradox: α-Klotho Levels and Smoking Intensity. Lung. 2017; 195(1):53-7. [DOI:10.1007/s00408-016-9944-6] [PMID]
Pace TW, Mletzko TC, Alagbe O, Musselman DL, Nemeroff CB, Miller AH, et al. Increased stress-induced inflammatory responses in male patients with major depression and increased early life stress. American Journal of Psychiatry. 2006; 163(9):1630-3. [DOI:10.1176/ajp.2006.163.9.1630] [PMID]
Nakanishi K, Nishida M, Yamamoto R, Koseki M, Moriyama T, Yamauchi-Takihara K. An implication of Klotho-related molecules in different smoking-related health outcomes between men and women. Clinica Chimica Acta. 2018; 476:44-8. [DOI:10.1016/j.cca.2017.11.007] [PMID]
Lam-Rachlin J, Romero R, Korzeniewski SJ, Schwartz AG, Chaemsaithong P, Hernandez-Andrade E, et al. Infection and smoking are associated with decreased plasma concentration of the anti-aging protein, α-klothoa. Journal of Perinatal Medicine. 2013; 41(5):581-94. [DOI:10.1515/jpm-2013-0084] [PMID] [PMCID]
Layne K, Wood DM. Opioid poisoning. Medicine. 2020; 48(3):211-3. [DOI:10.1016/j.mpmed.2019.12.018]
Alinejad S, Zamani N, Abdollahi M, Mehrpour O. A narrative review of acute adult poisoning in Iran. Iranian Journal of Medical Sciences. 2017; 42(4):327-46. [PMID]
Tremblay A, Bandi V. Impact of body mass index on outcomes following critical care. Chest. 2003; 123(4):1202-7. [DOI:10.1378/chest.123.4.1202] [PMID]
Foy CG, Bell RA, Farmer DF, Goff DC Jr, Wagenknecht LE. Smoking and incidence of diabetes among US adults: Findings from the Insulin Resistance Atherosclerosis Study. Diabetes Care. 2005; 28(10):2501-7. [DOI:10.2337/diacare.28.10.2501] [PMID]
Wang Q, Su W, Shen Z, Wang R. Correlation between soluble α-Klotho and renal function in patients with chronic kidney disease: A review and meta-analysis. BioMed Research International. 2018; 2018:9481475. [DOI:10.1155/2018/9481475] [PMID] [PMCID]
Landry T, Li P, Shookster D, Jiang Z, Li H, Laing BT, et al. Centrally circulating α-klotho inversely correlates with human obesity and modulates arcuate cell populations in mice. Molecular Metabolism. 2021; 44:101136. [DOI:10.1016/j.molmet.2020.101136] [PMID] [PMCID]
Lee J, Kim D, Lee HJ, Choi JY, Min JY, Min KB. Association between serum klotho levels and cardiovascular disease risk factors in older adults. BMC Cardiovascular Disorders. 2022; 22(1):442. [DOI:10.1186/s12872-022-02885-2] [PMID] [PMCID]
Alkalbani M, Prabhu G, Lagbo J, Qayyum R. Serum Klotho and pulse pressure; insight from NHANES. International Journal of Cardiology. 2022; 355:54-8. [DOI:10.1016/j.ijcard.2022.02.021] [PMID]
Yao Y, Long Y, Du FW, Zhao Y, Luo XB. Association between serum cotinine and α-Klotho levels among adults: Findings from the National Health and Nutrition Examination Survey 2007-2016. Tobacco Induced Diseases. 2022; 20:57.[DOI:10.18332/tid/144622] [PMID] [PMCID]
Yazici AB, Guzel D, Kurt EM, Turkmen B, Yazici E. Klotho, BDNF, NGF, GDNF levels and related factors in withdrawal period in chronic cannabinoid users. Indian Journal of Clinical Biochemistry. 2022; 37(2):139-48. [DOI:10.1007/s12291-021-00959-0] [PMID] [PMCID]
- Abstract Viewed: 138 times
- pdf Downloaded: 112 times