Proteome evaluation of human cystic echinococcosis sera using two dimensional gel electrophoresis
Gastroenterology and Hepatology from Bed to Bench,
Vol. 11 No. 1 (2018),
6 February 2018
https://doi.org/10.22037/ghfbb.v0i0.1253
Abstract
Aim: Detection of protein expression changes in human cystic echinococcosis sera by 2D gel electrophoresis.
Background: Diagnosis and successful treatment of cystic echinococcosis (CE) is a major challenge, up to now. Identification of related expressed proteins using proteomics tools and bioinformatics analysis of patients’ sera have not been investigated, so far.
Methods: Sera from eight confirmed CE patients and three healthy controls were collected, tested by 2-DE for total protein separation of serum and analyzed using proteomics and bioinformatics methods. The gels were stained by Coomassie blue followed by scan imaging of the gels. The protein spots in each gel were analyzed using progenesis same spots software. Proteins names were obtained from TagIdent server.
Results: A total of 263 protein spots with different expression were detected in both normal and diseased samples. Comparison between diseased and normal gels showed the expression of 45 up-regulated protein spots with fold?2 in diseased gel of which 10 were new proteins with statistical difference by normal gel (p-value<0.05). On the other hand, the expression of 50 down-regulated protein spots were observed of which 11 proteins have been suppressed. Clustering of all detected sera proteins (263) using correlation analysis, divided the proteins into 2 clusters based on up-regulated and down-regulated expression of proteins. Clustering results were approved by principal component analysis (PCA).
Conclusion: Significant protein expression changes in human CE sera which is demonstrable by application of proteomics and bioinformatics analysis makes it an impressing tool for diagnosis of CE patients.
Keywords: Proteomics, cystic echinococcosis, Cluster, Hydatid cyst, 2-D electrophoresis (2?DE).
(Please cite as: Sadjjadi FS, Rezaie-Tavirani M, Ahmadi NA, Sadjjadi SM, Zali H. Proteome evaluation of human cystic echinococcosis sera using two dimensional gel electrophoresis. Gastroenterol Hepatol Bed Bench 2018;11(1):75-82).
- Proteomics
- cystic echinococcosis
- Cluster
- Hydatid cyst
- 2-D electrophoresis (2?DE)
How to Cite
References
Sadjjadi SM. Present situation of echinococcosis in the Middle East and Arabic North Africa. Parasitol Int 2006;55(SUPPL.):S197-S202.
Ahmadi N, Dalimi A. Characterization of Echinococcus granulosus isolates from human, sheep and camel in Iran. Infec Genet Evol 2006;6(2):85-90.
Ahmadi NA, Hamidi M. A retrospective analysis of human cystic echinococcosis in Hamedan province, an endemic region of Iran. Ann Trop Med Parasitol. 2008;102(7):603–609.
Barnes TS, Deplazes P, Gottstein B, Jenkins DJ, Mathis A, Siles-Lucas M, et al. Challenges for diagnosis and control of cystic hydatid disease. Acta Trop 2012;123(1):1-7.
Eckert J, Deplazes P. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev 2004;17(1):107-135.
Tamarozzi F, Sako Y, Ito A, Piccoli L, Grisolìa A, Itoh S, et al. Recombinant AgB8/1 ELISA test vs. commercially available IgG ELISA test in the diagnosis of cystic echinococcosis. Parasite Immunol 2013;35(12):433-440.
Ahmadi NA, Badi F. Diagnostic, surgical, and medicinal approaches of human cystic echinococcosis: A review of 203 cases in Tehran, Iran. Infect Dis Clin Pract 2012;20(6):390-393.
Benabid M, Galai Y, Nouira R, Bouchoucha S, Bouratbine A, Aoun K. Contribution of specific anti-hydatid IgG subclasses in the diagnosis of echinococcosis primary infection and relapses. Clin Lab 2013;59(3-4):293-298.
Mohammadzadeh T, Sako Y, Sadjjadi SM, Sarkari B, Ito A. Comparison of the usefulness of hydatid cyst fluid, native antigen B and recombinant antigen B8/1 for serological diagnosis of cystic echinococcosis. Trans R Soc Trop Med Hyg 2012;106(6):371-375.
Khalilpour A, Kilic T, Khalilpour S, Álvarez MM, Yazdi IK. Proteomic-based biomarker discovery for development of next generation diagnostics. Appl Microbiol Biotechnol 2017;101(2):475-491.
Celis JE, Gromov P. 2D protein electrophoresis: can it be perfected? Curr Opin Biotechnol. 1999;10(1):16-21.
Karp NA, Lilley KS. Design and analysis issues in quantitative proteomics studies. Proteomics 2007;7 Suppl 1:42-50.
Aziz A, Zhang W, Li J, Loukas A, McManus DP, Mulvenna J. Proteomic characterisation of Echinococcus granulosus hydatid cyst fluid from sheep, cattle and humans. J Proteomics 2011;74(9):1560-1572.
Hidalgo C, García MP, Stoore C, Ramírez JP, Monteiro KM, Hellman U, et al. Proteomics analysis of Echinococcus granulosus protoscolex stage. Vet Parasitol 2016;218:43-45.
Longuespée R, Casadonte R, Kriegsmann M, Wandernoth P, Lisenko K, Mazzucchelli G, et al. Proteomic investigation of human cystic echinococcosis in the liver. Mol Biochem Parasitol 2017;211:9-14.
Cui S-, Xu L-, Zhang T, Xu M, Yao J, Fang C-, et al. Proteomic characterization of larval and adult developmental stages in Echinococcus granulosus reveals novel insight into host-parasite interactions. J Proteomics 2013;84:158-175.
Beranova-Giorgianni S. Proteome analysis by two-dimensional gel electrophoresis and mass spectrometry: Strengths and limitations. TrAC Trends Anal Chem 2003;22(5):273-281.
Lancashire L, Schmid O, Shah H, Ball G. Classification of bacterial species from proteomic data using combinatorial approaches incorporating artificial neural networks, cluster analysis and principal components analysis. Bioinformatics 2005;21(10):2191-2199.
Zhang W, Wen H, Li J, Lin R, McManus DP. Immunology and immunodiagnosis of cystic echinococcosis: An update. Clin Dev Immunol 2012;2012.
Zheng H, Zhang W, Zhang L, Zhang Z, Li J, Lu G, et al. The genome of the hydatid tapeworm Echinococcus granulosus. Nat Genet 2013;45(10):1168-1175.
Çelik T, Akarsu GA, Güngör Ç, Çolak C, Örmeci N. Evaluation of antibodies against hydatid cyst fluid antigens in the post-treatment follow-up of cystic echinococcosis patients. Med Sci Monit 2009;15(4):CR170-CR176.
Sun Z-, Zhu Y, Wang F-, Chen R, Peng T, Fan Z-, et al. Serum proteomic-based analysis of pancreatic carcinoma for the identification of potential cancer biomarkers. Biochim Biophys Acta Proteins Proteomics 2007;1774(6):764-771.
Takahashi E, Unoki-Kubota H, Shimizu Y, Okamura T, Iwata W, Kajio H, et al. Proteomic analysis of serum biomarkers for prediabetes using the Long-Evans Agouti rat, a spontaneous animal model of type 2 diabetes mellitus. J Diabetes Invest 2017;8(5):661-671.
Ebert MPA, Krüger S, Fogeron M-, Lamer S, Chen J, Pross M, et al. Overexpression of cathepsin B in gastric cancer identified by proteome analysis. Proteomics 2005;5(6):1693-1704.
Virginio VG, Monteiro KM, Drumond F, De Carvalho MO, Vargas DM, Zaha A, et al. Excretory/secretory products from in vitro-cultured Echinococcus granulosus protoscoleces. Mol Biochem Parasitol 2012;183(1):15-22.
Srivastava R, Ray S, Vaibhav V, Gollapalli K, Jhaveri T, Taur S, et al. Serum profiling of leptospirosis patients to investigate proteomic alterations. J Proteomics 2012;76:56-68.
Saia-Cereda VM, Aquino A, Guest PC, Martins-de-Souza D. Two-dimensional gel electrophoresis: A reference protocol. Adv Exp Med Biol 2017;974:175-182.
Da'dara AA, Siddons G, Icaza M, Wang Q, Skelly PJ. How schistosomes alter the human serum proteome. Mol Biochem Parasitol 2017;215:40-46.
Gouthamchandra K, Kumar A, Shwetha S, Mukherjee A, Chandra M, Ravishankar B, et al. Serum proteomics of hepatitis C virus infection reveals retinol-binding protein 4 as a novel regulator. J Gen Virol 2014;95(PART 8):1654-1667.
Chemale G, Van Rossum AJ, Jefferies JR, Barrett J, Brophy PM, Ferreira HB, et al. Proteomic analysis of the larval stage of the parasite Echinococcus granulosus: Causative agent of cystic hydatid disease. Proteomics 2003;3(8):1633-1636.
Wang Y, Xiao D, Shen Y, Han X, Zhao F, Li X, et al. Proteomic analysis of the excretory/secretory products and antigenic proteins of Echinococcus granulosus adult worms from infected dogs. BMC Vet Res 2015;11(1).
Wilkins MR, Gasteiger E, Tonella L, Ou K, Tyler M, Sanchez J-, et al. Protein identification with N and C-terminal sequence tags in proteome projects. J Mol Biol 1998;278(3):599-608.
Orengo CA, Michie AD, Jones S, Jones DT, Swindells MB, Thornton JM. CATH - A hierarchic classification of protein domain structures. Structure 1997;5(8):1093-1108.
Hashemi M, Pooladi M, Abad SKR, Movafagh A, Entezari M. Proteomic cluster analysis of malignant gliomas in humans. Middle East J Cancer 2016;7(1):31-40.
Alreshidi MM, Dunstan RH, Macdonald MM, Smith ND, Gottries J, Roberts TK. Metabolomic and proteomic responses of Staphylococcus aureus to prolonged cold stress. J Proteomics 2015;121:44-55.
- Abstract Viewed: 366 times
- PDF Downloaded: 176 times